{"id":"https://openalex.org/W2139188291","doi":"https://doi.org/10.1093/nar/gkv734","title":"Transcription yield of fully 2\u2032-modified RNA can be increased by the addition of thermostabilizing mutations to T7 RNA polymerase mutants","display_name":"Transcription yield of fully 2\u2032-modified RNA can be increased by the addition of thermostabilizing mutations to T7 RNA polymerase mutants","publication_year":2015,"publication_date":"2015-07-24","ids":{"openalex":"https://openalex.org/W2139188291","doi":"https://doi.org/10.1093/nar/gkv734","mag":"2139188291","pmid":"https://pubmed.ncbi.nlm.nih.gov/26209133","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/4551944"},"language":"en","primary_location":{"id":"doi:10.1093/nar/gkv734","doi":"https://doi.org/10.1093/nar/gkv734","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/gkv734","pdf_url":"https://academic.oup.com/nar/article-pdf/43/15/7480/17434623/gkv734.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://academic.oup.com/nar/article-pdf/43/15/7480/17434623/gkv734.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5085684770","display_name":"Adam J. Meyer","orcid":"https://orcid.org/0000-0001-5455-3418","observed_orcids":["https://orcid.org/0000-0001-5455-3418"]},"institutions":[{"id":"https://openalex.org/I86519309","display_name":"The University of Texas at Austin","ror":"https://ror.org/00hj54h04","country_code":"US","type":"education","lineage":["https://openalex.org/I86519309"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Adam J. Meyer","raw_affiliation_strings":["Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA andy.ellington@mail.utexas.edu","Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA andy.ellington@mail.utexas.edu","institution_ids":["https://openalex.org/I86519309"]},{"raw_affiliation_string":"Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA","institution_ids":["https://openalex.org/I86519309"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5036817888","display_name":"Daniel J. Garry","orcid":"https://orcid.org/0000-0002-8970-7365","observed_orcids":["https://orcid.org/0000-0002-8970-7365"]},"institutions":[{"id":"https://openalex.org/I86519309","display_name":"The University of Texas at Austin","ror":"https://ror.org/00hj54h04","country_code":"US","type":"education","lineage":["https://openalex.org/I86519309"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Daniel J. Garry","raw_affiliation_strings":["Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA","institution_ids":["https://openalex.org/I86519309"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5087027110","display_name":"Bradley Hall","orcid":"https://orcid.org/0009-0001-1312-0832","observed_orcids":["https://orcid.org/0009-0001-1312-0832","https://orcid.org/0009-0008-4274-9796"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Bradley Hall","raw_affiliation_strings":["Altermune Technologies, LLC, Irvine, CA 92606, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Altermune Technologies, LLC, Irvine, CA 92606, USA","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5070440074","display_name":"Michelle Byrom","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I86519309","display_name":"The University of Texas at Austin","ror":"https://ror.org/00hj54h04","country_code":"US","type":"education","lineage":["https://openalex.org/I86519309"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michelle M. Byrom","raw_affiliation_strings":["Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA","institution_ids":["https://openalex.org/I86519309"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5011098073","display_name":"Hannah G. McDonald","orcid":"https://orcid.org/0000-0002-4523-907X","observed_orcids":["https://orcid.org/0000-0002-4523-907X"]},"institutions":[{"id":"https://openalex.org/I86519309","display_name":"The University of Texas at Austin","ror":"https://ror.org/00hj54h04","country_code":"US","type":"education","lineage":["https://openalex.org/I86519309"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Hannah G. McDonald","raw_affiliation_strings":["Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA","institution_ids":["https://openalex.org/I86519309"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5045709526","display_name":"Yang Xu","orcid":"https://orcid.org/0000-0003-1670-6131","observed_orcids":["https://orcid.org/0000-0003-1670-6131"]},"institutions":[{"id":"https://openalex.org/I55302922","display_name":"The University of Texas Medical Branch at Galveston","ror":"https://ror.org/016tfm930","country_code":"US","type":"education","lineage":["https://openalex.org/I55302922"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Xu Yang","raw_affiliation_strings":["Department of Pharmacology and Toxicology, Sealy Center for Structural Biology, University of Texas Medical Branch, Galveston, TX 77555, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmacology and Toxicology, Sealy Center for Structural Biology, University of Texas Medical Branch, Galveston, TX 77555, USA","institution_ids":["https://openalex.org/I55302922"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5091323236","display_name":"Yuhui Whitney Yin","orcid":"https://orcid.org/0000-0001-7063-9385","observed_orcids":["https://orcid.org/0000-0001-7063-9385"]},"institutions":[{"id":"https://openalex.org/I55302922","display_name":"The University of Texas Medical Branch at Galveston","ror":"https://ror.org/016tfm930","country_code":"US","type":"education","lineage":["https://openalex.org/I55302922"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Y. Whitney Yin","raw_affiliation_strings":["Department of Pharmacology and Toxicology, Sealy Center for Structural Biology, University of Texas Medical Branch, Galveston, TX 77555, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Pharmacology and Toxicology, Sealy Center for Structural Biology, University of Texas Medical Branch, Galveston, TX 77555, USA","institution_ids":["https://openalex.org/I55302922"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5089627980","display_name":"Andrew D. Ellington","orcid":"https://orcid.org/0000-0001-6246-5338","observed_orcids":["https://orcid.org/0000-0001-6246-5338"]},"institutions":[{"id":"https://openalex.org/I86519309","display_name":"The University of Texas at Austin","ror":"https://ror.org/00hj54h04","country_code":"US","type":"education","lineage":["https://openalex.org/I86519309"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Andrew D. Ellington","raw_affiliation_strings":["Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, TX 78712, USA Center for Systems & Synthetic Biology, University of Texas at Austin, Austin, TX 78712, USA","Center for Systems & Synthetic Biology, University of Texas at Austin, Austin, TX 78712, USA","Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, TX 78712, USA","Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, TX 78712, USA Center for Systems & Synthetic Biology, University of Texas at Austin, Austin, TX 78712, USA","institution_ids":["https://openalex.org/I86519309"]},{"raw_affiliation_string":"Center for Systems & Synthetic Biology, University of Texas at Austin, Austin, TX 78712, USA","institution_ids":["https://openalex.org/I86519309"]},{"raw_affiliation_string":"Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, TX 78712, USA","institution_ids":["https://openalex.org/I86519309"]},{"raw_affiliation_string":"Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA","institution_ids":["https://openalex.org/I86519309"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5085684770"],"corresponding_institution_ids":["https://openalex.org/I86519309"],"apc_list":{"value":3992,"currency":"USD","value_usd":3992},"apc_paid":{"value":3992,"currency":"USD","value_usd":3992},"fwci":2.8916,"has_fulltext":true,"cited_by_count":88,"citation_normalized_percentile":{"value":0.91746514,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":99},"biblio":{"volume":"43","issue":"15","first_page":"7480","last_page":"7488"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.9761000275611877,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.9761000275611877,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.009399999864399433,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.005400000140070915,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/t7-rna-polymerase","display_name":"T7 RNA polymerase","score":0.9929999709129333},{"id":"https://openalex.org/keywords/2-o-methyl-rna","display_name":"2'-O-methyl RNA","score":0.8510000109672546},{"id":"https://openalex.org/keywords/protein-stability","display_name":"protein stability","score":0.47200000286102295},{"id":"https://openalex.org/keywords/thermal-tolerance","display_name":"heat tolerance","score":0.5339999794960022},{"id":"https://openalex.org/keywords/modified-nucleotides","display_name":"modified nucleotides","score":0.4749999940395355}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8352711796760559},{"id":"https://openalex.org/C2780048736","wikidata":"https://www.wikidata.org/wiki/Q418713","display_name":"T7 RNA polymerase","level":5,"score":0.7496855854988098},{"id":"https://openalex.org/C82381507","wikidata":"https://www.wikidata.org/wiki/Q416878","display_name":"Polymerase","level":3,"score":0.7089449167251587},{"id":"https://openalex.org/C2776449523","wikidata":"https://www.wikidata.org/wiki/Q272631","display_name":"RNA polymerase","level":4,"score":0.6454710960388184},{"id":"https://openalex.org/C179926584","wikidata":"https://www.wikidata.org/wiki/Q207714","display_name":"Transcription (linguistics)","level":2,"score":0.629487931728363},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.5926985740661621},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.554682731628418},{"id":"https://openalex.org/C41258723","wikidata":"https://www.wikidata.org/wiki/Q2919111","display_name":"RNA-dependent RNA polymerase","level":4,"score":0.5522982478141785},{"id":"https://openalex.org/C25281209","wikidata":"https://www.wikidata.org/wiki/Q3502188","display_name":"RNA polymerase I","level":5,"score":0.46858590841293335},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4627869427204132},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.3717277944087982},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3612685799598694},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.287651926279068},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.18798381090164185},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.07844927906990051},{"id":"https://openalex.org/C2776441376","wikidata":"https://www.wikidata.org/wiki/Q165028","display_name":"Bacteriophage","level":4,"score":0.05224168300628662},{"id":"https://openalex.org/C138885662","wikidata":"https://www.wikidata.org/wiki/Q5891","display_name":"Philosophy","level":0,"score":0.0},{"id":"https://openalex.org/C41895202","wikidata":"https://www.wikidata.org/wiki/Q8162","display_name":"Linguistics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D004795","descriptor_name":"Enzyme Stability","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012313","descriptor_name":"RNA","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D012313","descriptor_name":"RNA","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D012321","descriptor_name":"DNA-Directed RNA Polymerases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D012321","descriptor_name":"DNA-Directed RNA Polymerases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012321","descriptor_name":"DNA-Directed RNA Polymerases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D013379","descriptor_name":"Substrate Specificity","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013696","descriptor_name":"Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014158","descriptor_name":"Transcription, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D014764","descriptor_name":"Viral Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D014764","descriptor_name":"Viral Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014764","descriptor_name":"Viral Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false}],"locations_count":5,"locations":[{"id":"doi:10.1093/nar/gkv734","doi":"https://doi.org/10.1093/nar/gkv734","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/gkv734","pdf_url":"https://academic.oup.com/nar/article-pdf/43/15/7480/17434623/gkv734.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:3524842","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4551944","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:4551944","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4551944","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Res","raw_type":"Text"},{"id":"pmid:26209133","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/26209133","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic acids research","raw_type":"Journal Article"},{"id":"pmh:oai:osti.gov:1234731","doi":null,"is_oa":true,"landing_page_url":"https://www.osti.gov/biblio/1234731","pdf_url":"https://www.osti.gov/servlets/purl/1234731","source":{"id":"https://openalex.org/S4306402487","display_name":"OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I139351228","host_organization_name":"Office of Scientific and Technical Information","host_organization_lineage":["https://openalex.org/I139351228"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"doi:10.1093/nar/gkv734","doi":"https://doi.org/10.1093/nar/gkv734","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/gkv734","pdf_url":"https://academic.oup.com/nar/article-pdf/43/15/7480/17434623/gkv734.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1409003578","display_name":null,"funder_award_id":"GM 083703","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G1616813121","display_name":"Center for Innovation in Point of Care Technologies for the Future of Cancer Care","funder_award_id":"U54EB015403","funder_id":"https://openalex.org/F4320337363","funder_display_name":"National Institute of Biomedical Imaging and Bioengineering"},{"id":"https://openalex.org/G1841188276","display_name":"Dissecting dual function of Pol gamma in mtDNA replication and oxidative damage r","funder_award_id":"R01GM110591","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G6200983748","display_name":"Studies of  toxicities of HIV RT inhibitors to human mitochondrial DNA polymerase","funder_award_id":"R01GM083703","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"},{"id":"https://openalex.org/F4320337363","display_name":"National Institute of Biomedical Imaging and Bioengineering","ror":"https://ror.org/00372qc85"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2139188291.pdf","grobid_xml":"https://content.openalex.org/works/W2139188291.grobid-xml"},"referenced_works_count":42,"referenced_works":["https://openalex.org/W1488701566","https://openalex.org/W1504746908","https://openalex.org/W1754156922","https://openalex.org/W1892050356","https://openalex.org/W1912230311","https://openalex.org/W1964529071","https://openalex.org/W1965277435","https://openalex.org/W1967798978","https://openalex.org/W1971377724","https://openalex.org/W1977723400","https://openalex.org/W1988625445","https://openalex.org/W2003890287","https://openalex.org/W2005381620","https://openalex.org/W2008819279","https://openalex.org/W2021928977","https://openalex.org/W2023373190","https://openalex.org/W2036332544","https://openalex.org/W2045895711","https://openalex.org/W2048599158","https://openalex.org/W2053762237","https://openalex.org/W2055424388","https://openalex.org/W2057974881","https://openalex.org/W2058102458","https://openalex.org/W2059346730","https://openalex.org/W2060329684","https://openalex.org/W2064036940","https://openalex.org/W2067167857","https://openalex.org/W2068202842","https://openalex.org/W2071938896","https://openalex.org/W2076769977","https://openalex.org/W2084120903","https://openalex.org/W2104525153","https://openalex.org/W2111066814","https://openalex.org/W2118265845","https://openalex.org/W2120145250","https://openalex.org/W2134432657","https://openalex.org/W2140012758","https://openalex.org/W2142972231","https://openalex.org/W2143313350","https://openalex.org/W2149649955","https://openalex.org/W2160082846","https://openalex.org/W2164517655"],"related_works":["https://openalex.org/W2036332544","https://openalex.org/W4410478548","https://openalex.org/W2102541127","https://openalex.org/W7198857904","https://openalex.org/W7169808625","https://openalex.org/W7148192534","https://openalex.org/W7051512602","https://openalex.org/W2766240151","https://openalex.org/W2794209700","https://openalex.org/W7134819124"],"abstract_inverted_index":{"On":[0],"average,":[1],"mutations":[2,70],"are":[3],"deleterious":[4],"to":[5,11,41,62,73,99],"proteins.":[6],"Mutations":[7],"conferring":[8],"new":[9],"function":[10],"a":[12,30],"protein":[13,20],"often":[14],"come":[15],"at":[16],"the":[17,28,58,75,84],"expense":[18],"of":[19,32,78,86],"folding":[21],"or":[22,39],"stability,":[23],"reducing":[24],"overall":[25],"activity.":[26],"Over":[27],"years,":[29],"panel":[31],"T7":[33,79],"RNA":[34,80,103],"polymerases":[35],"have":[36,51],"been":[37],"designed":[38],"evolved":[40],"accept":[42],"nucleotides":[43],"with":[44,88,104],"modified":[45,49,102],"ribose":[46],"moieties.":[47],"These":[48],"RNAs":[50],"proven":[52],"useful,":[53],"especially":[54],"in":[55],"vivo,":[56],"but":[57],"transcriptional":[59],"yields":[60,105],"tend":[61],"be":[63,97],"quite":[64],"low.":[65],"Here":[66],"we":[67],"show":[68],"that":[69],"previously":[71],"shown":[72],"increase":[74,83],"thermal":[76],"tolerance":[77],"polymerase":[81,94],"can":[82,96],"activity":[85],"mutants":[87,95],"expanded":[89],"substrate":[90],"range.":[91],"The":[92],"resulting":[93],"used":[98],"generate":[100],"2'-O-methyl":[101],"much":[106],"higher":[107],"than":[108],"enzymes":[109],"currently":[110],"employed.":[111]},"counts_by_year":[{"year":2026,"cited_by_count":9},{"year":2025,"cited_by_count":9},{"year":2024,"cited_by_count":12},{"year":2023,"cited_by_count":4},{"year":2022,"cited_by_count":7},{"year":2021,"cited_by_count":8},{"year":2020,"cited_by_count":10},{"year":2019,"cited_by_count":9},{"year":2018,"cited_by_count":8},{"year":2017,"cited_by_count":7},{"year":2016,"cited_by_count":4},{"year":2015,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}