{"id":"https://openalex.org/W2139132507","doi":"https://doi.org/10.1155/2013/140469","title":"Combination ofERG9Repression and Enzyme Fusion Technology for Improved Production of Amorphadiene inSaccharomyces cerevisiae","display_name":"Combination ofERG9Repression and Enzyme Fusion Technology for Improved Production of Amorphadiene inSaccharomyces cerevisiae","publication_year":2013,"publication_date":"2013-01-01","ids":{"openalex":"https://openalex.org/W2139132507","doi":"https://doi.org/10.1155/2013/140469","mag":"2139132507","pmid":"https://pubmed.ncbi.nlm.nih.gov/24282652","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/3826331"},"language":"en","primary_location":{"id":"doi:10.1155/2013/140469","doi":"https://doi.org/10.1155/2013/140469","is_oa":true,"landing_page_url":"https://doi.org/10.1155/2013/140469","pdf_url":"https://downloads.hindawi.com/journals/jamc/2013/140469.pdf","source":{"id":"https://openalex.org/S4210240219","display_name":"Journal of Analytical Methods in Chemistry","issn_l":"2090-8865","issn":["2090-8865","2090-8873"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4310319869","host_organization_name":"Hindawi Publishing Corporation","host_organization_lineage":["https://openalex.org/P4310319869","https://openalex.org/P4310320595"],"host_organization_lineage_names":["Hindawi Publishing Corporation","Wiley"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Analytical Methods in Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://downloads.hindawi.com/journals/jamc/2013/140469.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5049476844","display_name":"Rama Raju Baadhe","orcid":"https://orcid.org/0000-0002-2958-0753","observed_orcids":["https://orcid.org/0000-0002-2958-0753"]},"institutions":[{"id":"https://openalex.org/I121750182","display_name":"National Institute of Technology Warangal","ror":"https://ror.org/017ebfz38","country_code":"IN","type":"education","lineage":["https://openalex.org/I121750182"]}],"countries":["IN"],"is_corresponding":true,"raw_author_name":"Rama Raju Baadhe","raw_affiliation_strings":["Department of Biotechnology, National Institute of Technology, Warangal 506004, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biotechnology, National Institute of Technology, Warangal 506004, India","institution_ids":["https://openalex.org/I121750182"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5089904528","display_name":"Naveen Kumar Mekala","orcid":"https://orcid.org/0000-0002-5343-8167","observed_orcids":["https://orcid.org/0000-0002-5343-8167"]},"institutions":[{"id":"https://openalex.org/I121750182","display_name":"National Institute of Technology Warangal","ror":"https://ror.org/017ebfz38","country_code":"IN","type":"education","lineage":["https://openalex.org/I121750182"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Naveen Kumar Mekala","raw_affiliation_strings":["Department of Biotechnology, National Institute of Technology, Warangal 506004, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biotechnology, National Institute of Technology, Warangal 506004, India","institution_ids":["https://openalex.org/I121750182"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5001653077","display_name":"Sreenivasa Rao Parcha","orcid":"https://orcid.org/0000-0003-1932-7292","observed_orcids":["https://orcid.org/0000-0003-1932-7292"]},"institutions":[{"id":"https://openalex.org/I121750182","display_name":"National Institute of Technology Warangal","ror":"https://ror.org/017ebfz38","country_code":"IN","type":"education","lineage":["https://openalex.org/I121750182"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Sreenivasa Rao Parcha","raw_affiliation_strings":["Department of Biotechnology, National Institute of Technology, Warangal 506004, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biotechnology, National Institute of Technology, Warangal 506004, India","institution_ids":["https://openalex.org/I121750182"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5108821592","display_name":"Yalavarthy Prameela Devi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I28210344","display_name":"Kakatiya University","ror":"https://ror.org/012zpbg81","country_code":"IN","type":"education","lineage":["https://openalex.org/I28210344"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Yalavarthy Prameela Devi","raw_affiliation_strings":["Department of Zoology, Kakatiya University, Warangal, Andhra Pradesh 506009, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Zoology, Kakatiya University, Warangal, Andhra Pradesh 506009, India","institution_ids":["https://openalex.org/I28210344"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5049476844"],"corresponding_institution_ids":["https://openalex.org/I121750182"],"apc_list":{"value":2160,"currency":"USD","value_usd":2160},"apc_paid":{"value":2160,"currency":"USD","value_usd":2160},"fwci":0.597,"has_fulltext":true,"cited_by_count":20,"citation_normalized_percentile":{"value":0.68511703,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":98},"biblio":{"volume":"2013","issue":null,"first_page":"1","last_page":"8"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11882","display_name":"Plant biochemistry and biosynthesis","score":0.9872999787330627,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11882","display_name":"Plant biochemistry and biosynthesis","score":0.9872999787330627,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.010099999606609344,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T14150","display_name":"Biochemical and biochemical processes","score":0.0006000000284984708,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/saccharomyces-cerevisiae","display_name":"Saccharomyces cerevisiae","score":0.8799999952316284},{"id":"https://openalex.org/keywords/farnesyl-pyrophosphate-synthase","display_name":"farnesyl diphosphate synthase","score":0.925000011920929},{"id":"https://openalex.org/keywords/artemisia-annua","display_name":"Artemisia annua","score":0.7509999871253967},{"id":"https://openalex.org/keywords/mevalonate-pathway","display_name":"mevalonate pathway","score":0.8809999823570251},{"id":"https://openalex.org/keywords/heterologous-biosynthesis","display_name":"heterologous biosynthesis","score":0.7059999704360962},{"id":"https://openalex.org/keywords/farnesyl-pyrophosphate","display_name":"farnesyl pyrophosphate","score":0.8389999866485596}],"concepts":[{"id":"https://openalex.org/C2777576037","wikidata":"https://www.wikidata.org/wiki/Q719725","display_name":"Saccharomyces cerevisiae","level":3,"score":0.8001580238342285},{"id":"https://openalex.org/C2779222958","wikidata":"https://www.wikidata.org/wiki/Q45422","display_name":"Yeast","level":2,"score":0.7876124382019043},{"id":"https://openalex.org/C2779982574","wikidata":"https://www.wikidata.org/wiki/Q14913010","display_name":"Mevalonate pathway","level":4,"score":0.7528412938117981},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.7059463858604431},{"id":"https://openalex.org/C2779691961","wikidata":"https://www.wikidata.org/wiki/Q1308044","display_name":"Artemisia annua","level":5,"score":0.6964710354804993},{"id":"https://openalex.org/C112243037","wikidata":"https://www.wikidata.org/wiki/Q22327117","display_name":"ATP synthase","level":3,"score":0.6653948426246643},{"id":"https://openalex.org/C2775933503","wikidata":"https://www.wikidata.org/wiki/Q25113781","display_name":"Farnesyl diphosphate synthase","level":4,"score":0.63846355676651},{"id":"https://openalex.org/C553450214","wikidata":"https://www.wikidata.org/wiki/Q851162","display_name":"Biosynthesis","level":3,"score":0.46139809489250183},{"id":"https://openalex.org/C108305142","wikidata":"https://www.wikidata.org/wiki/Q3062286","display_name":"Heterologous expression","level":4,"score":0.447261780500412},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4323327839374542},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.40920329093933105},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.3887918293476105},{"id":"https://openalex.org/C2776120307","wikidata":"https://www.wikidata.org/wiki/Q426921","display_name":"Artemisinin","level":4,"score":0.19888263940811157},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.14842566847801208},{"id":"https://openalex.org/C2778371730","wikidata":"https://www.wikidata.org/wiki/Q311383","display_name":"Plasmodium falciparum","level":3,"score":0.10964635014533997},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.09807810187339783},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.0},{"id":"https://openalex.org/C2778048844","wikidata":"https://www.wikidata.org/wiki/Q12156","display_name":"Malaria","level":2,"score":0.0}],"mesh":[],"locations_count":5,"locations":[{"id":"doi:10.1155/2013/140469","doi":"https://doi.org/10.1155/2013/140469","is_oa":true,"landing_page_url":"https://doi.org/10.1155/2013/140469","pdf_url":"https://downloads.hindawi.com/journals/jamc/2013/140469.pdf","source":{"id":"https://openalex.org/S4210240219","display_name":"Journal of Analytical Methods in Chemistry","issn_l":"2090-8865","issn":["2090-8865","2090-8873"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4310319869","host_organization_name":"Hindawi Publishing Corporation","host_organization_lineage":["https://openalex.org/P4310319869","https://openalex.org/P4310320595"],"host_organization_lineage_names":["Hindawi Publishing Corporation","Wiley"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Analytical Methods in Chemistry","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:2823532","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3826331","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:3826331","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3826331","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Anal Methods Chem","raw_type":"Text"},{"id":"pmid:24282652","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/24282652","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of analytical methods in chemistry","raw_type":"Journal Article"},{"id":"pmh:oai:doaj.org/article:1efa2728bc944b5a8bb355565dd9f033","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/1efa2728bc944b5a8bb355565dd9f033","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Journal of Analytical Methods in Chemistry, Vol 2013 (2013)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.1155/2013/140469","doi":"https://doi.org/10.1155/2013/140469","is_oa":true,"landing_page_url":"https://doi.org/10.1155/2013/140469","pdf_url":"https://downloads.hindawi.com/journals/jamc/2013/140469.pdf","source":{"id":"https://openalex.org/S4210240219","display_name":"Journal of Analytical Methods in Chemistry","issn_l":"2090-8865","issn":["2090-8865","2090-8873"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","norway-1","poland-40","russia-white-list-3"],"host_organization":"https://openalex.org/P4310319869","host_organization_name":"Hindawi Publishing Corporation","host_organization_lineage":["https://openalex.org/P4310319869","https://openalex.org/P4310320595"],"host_organization_lineage_names":["Hindawi Publishing Corporation","Wiley"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Analytical Methods in Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2139132507.pdf","grobid_xml":"https://content.openalex.org/works/W2139132507.grobid-xml"},"referenced_works_count":36,"referenced_works":["https://openalex.org/W247156514","https://openalex.org/W1227699656","https://openalex.org/W1963173460","https://openalex.org/W1969251758","https://openalex.org/W1979420899","https://openalex.org/W1999560790","https://openalex.org/W2008258387","https://openalex.org/W2012321934","https://openalex.org/W2014177423","https://openalex.org/W2015987669","https://openalex.org/W2017324564","https://openalex.org/W2019281554","https://openalex.org/W2021933218","https://openalex.org/W2030168212","https://openalex.org/W2039438529","https://openalex.org/W2060782384","https://openalex.org/W2061700911","https://openalex.org/W2065439398","https://openalex.org/W2072369409","https://openalex.org/W2072911080","https://openalex.org/W2077837797","https://openalex.org/W2092226009","https://openalex.org/W2093573937","https://openalex.org/W2098545768","https://openalex.org/W2102865022","https://openalex.org/W2113501061","https://openalex.org/W2113661979","https://openalex.org/W2128897939","https://openalex.org/W2134923619","https://openalex.org/W2146402972","https://openalex.org/W2149202606","https://openalex.org/W2154797402","https://openalex.org/W2160657983","https://openalex.org/W2165034669","https://openalex.org/W2337763812","https://openalex.org/W4360753951"],"related_works":["https://openalex.org/W7095791273","https://openalex.org/W2071838425","https://openalex.org/W2066361534","https://openalex.org/W3087573915","https://openalex.org/W2416515335","https://openalex.org/W2332641124","https://openalex.org/W2128897939","https://openalex.org/W3093747932","https://openalex.org/W4412512672","https://openalex.org/W2392299547"],"abstract_inverted_index":{"The":[0],"yeast":[1,61,97,135,163],"strain":[2,171],"(Saccharomyces":[3],"cerevisiae)":[4],"MTCC":[5],"3157":[6],"was":[7,19,98,128,152],"selected":[8],"for":[9],"combinatorial":[10],"biosynthesis":[11],"of":[12,30,51,54,60,67,96,116,134,143],"plant":[13,37,144],"sesquiterpene":[14],"amorpha-4,11-diene.":[15],"Our":[16],"main":[17],"objective":[18],"to":[20,64,80,112],"overproduce":[21],"amorpha":[22],"4-11-diene,":[23],"which":[24],"is":[25,46],"a":[26,47,52],"key":[27],"precursor":[28],"molecule":[29],"artemisinin":[31],"(antimalarial":[32],"drug)":[33],"produced":[34],"naturally":[35],"in":[36,56,89,123,162],"Artemisia":[38],"annua":[39,147],"through":[40,120],"mevalonate":[41,58],"pathway.":[42],"Farnesyl":[43],"diphosphate":[44],"(FPP)":[45],"common":[48],"intermediate":[49,118],"metabolite":[50],"variety":[53],"compounds":[55],"the":[57,65,114,117],"pathway":[59],"and":[62,70,72,130,158,166],"leads":[63],"production":[66,151],"ergosterols,":[68],"dolichol":[69],"ubiquinone,":[71],"so":[73],"forth.":[74],"In":[75],"our":[76],"studies,":[77],"FPP":[78,119],"converted":[79],"amorphadiene":[81,86,140,150],"(AD)":[82],"by":[83,105,154],"expressing":[84],"heterologous":[85],"synthase":[87,132,141],"(ADS)":[88,142],"yeast.":[90],"First,":[91],"ERG9":[92],"(squalane":[93],"synthase)":[94],"promoter":[95,104],"replaced":[99],"with":[100,139,169],"repressible":[101],"methionine":[102],"(MET3)":[103],"using":[106],"bipartite":[107],"gene":[108],"fusion":[109,125],"method.":[110],"Further":[111],"overcome":[113],"loss":[115],"competitive":[121],"pathways":[122],"yeast,":[124],"protein":[126],"technology":[127],"adopted":[129],"farnesyldiphosphate":[131],"(FPPS)":[133],"has":[136],"been":[137],"coupled":[138],"origin":[145],"(Artemisia":[146],"L.)":[148],"where":[149],"improved":[153],"2-fold":[155],"(11.2":[156],"mg/L)":[157,161],"4-fold":[159],"(25.02":[160],"strains":[164],"YCF-002":[165],"YCF-005":[167],"compared":[168],"control":[170],"YCF-AD":[172],"(5.5":[173],"mg/L),":[174],"respectively.":[175]},"counts_by_year":[{"year":2022,"cited_by_count":4},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":4},{"year":2017,"cited_by_count":3},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":1}],"updated_date":"2026-10-30T12:39:25.321706","created_date":"2016-06-24T00:00:00"}