{"id":"https://openalex.org/W2139131274","doi":"https://doi.org/10.1111/j.1550-7408.2004.tb00606.x","title":"Identification of Single Nucleotide Mutations That Prevent Developmentally Programmed DNA Elimination in Paramecium tetraurelia","display_name":"Identification of Single Nucleotide Mutations That Prevent Developmentally Programmed DNA Elimination in Paramecium tetraurelia","publication_year":2004,"publication_date":"2004-11-01","ids":{"openalex":"https://openalex.org/W2139131274","doi":"https://doi.org/10.1111/j.1550-7408.2004.tb00606.x","mag":"2139131274","pmid":"https://pubmed.ncbi.nlm.nih.gov/15666724"},"language":"en","primary_location":{"id":"doi:10.1111/j.1550-7408.2004.tb00606.x","doi":"https://doi.org/10.1111/j.1550-7408.2004.tb00606.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1550-7408.2004.tb00606.x","pdf_url":null,"source":{"id":"https://openalex.org/S73111969","display_name":"Journal of Eukaryotic Microbiology","issn_l":"1066-5234","issn":["1066-5234","1550-7408"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Eukaryotic Microbiology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5051819621","display_name":"Atsushi Matsuda","orcid":"https://orcid.org/0000-0003-0510-213X","observed_orcids":["https://orcid.org/0000-0003-0510-213X"]},"institutions":[{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"ATSUSHI MATSUDA","raw_affiliation_strings":["Department of Biochemistry, Purdue University, 175 South University Street, West Lafayette, Indiana 47907-2063, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Purdue University, 175 South University Street, West Lafayette, Indiana 47907-2063, USA","institution_ids":["https://openalex.org/I219193219"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109062429","display_name":"Kimberly M. Mayer","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I200870766","display_name":"Brookhaven National Laboratory","ror":"https://ror.org/02ex6cf31","country_code":"US","type":"facility","lineage":["https://openalex.org/I1330989302","https://openalex.org/I200870766","https://openalex.org/I39565521","https://openalex.org/I4210142672"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"KIMBERLY M. MAYER","raw_affiliation_strings":["Biology Department, Brookhaven National Laboratory, Upton, New York 11973"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biology Department, Brookhaven National Laboratory, Upton, New York 11973","institution_ids":["https://openalex.org/I200870766"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5082657792","display_name":"JAMES D. FORNEY","orcid":"https://orcid.org/0000-0002-6144-732X","observed_orcids":["https://orcid.org/0000-0002-6144-732X"]},"institutions":[{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"JAMES D. FORNEY","raw_affiliation_strings":["Department of Biochemistry, Purdue University, 175 South University Street, West Lafayette, Indiana 47907-2063"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Purdue University, 175 South University Street, West Lafayette, Indiana 47907-2063","institution_ids":["https://openalex.org/I219193219"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5082657792"],"corresponding_institution_ids":["https://openalex.org/I219193219"],"apc_list":{"value":3090,"currency":"USD","value_usd":3090},"apc_paid":null,"fwci":0.3908,"has_fulltext":false,"cited_by_count":8,"citation_normalized_percentile":{"value":0.73702347,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"51","issue":"6","first_page":"664","last_page":"669"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10434","display_name":"Chromosomal and Genetic Variations","score":0.73580002784729,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10434","display_name":"Chromosomal and Genetic Variations","score":0.73580002784729,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11879","display_name":"Protist diversity and phylogeny","score":0.18050000071525574,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.010700000450015068,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/paramecium-tetraurelia","display_name":"Paramecium tetraurelia","score":0.9010000228881836},{"id":"https://openalex.org/keywords/internal-eliminated-sequences","display_name":"internal eliminated sequences","score":0.9010000228881836},{"id":"https://openalex.org/keywords/somatic-differentiation","display_name":"somatic differentiation","score":0.5989999771118164},{"id":"https://openalex.org/keywords/point-mutations","display_name":"point mutations","score":0.7680000066757202},{"id":"https://openalex.org/keywords/inverted-terminal-repeats","display_name":"inverted terminal repeats","score":0.8289999961853027},{"id":"https://openalex.org/keywords/nucleotide-bias","display_name":"nucleotide bias","score":0.5889999866485596}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.9023697376251221},{"id":"https://openalex.org/C2780536333","wikidata":"https://www.wikidata.org/wiki/Q199456","display_name":"Paramecium","level":2,"score":0.8630593419075012},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.6677581071853638},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.6390178799629211},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.5216246843338013},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.4947340190410614},{"id":"https://openalex.org/C512185932","wikidata":"https://www.wikidata.org/wiki/Q28745","display_name":"Nucleotide","level":3,"score":0.47835439443588257},{"id":"https://openalex.org/C4918238","wikidata":"https://www.wikidata.org/wiki/Q121438","display_name":"Transposable element","level":4,"score":0.4701024889945984},{"id":"https://openalex.org/C84148353","wikidata":"https://www.wikidata.org/wiki/Q863908","display_name":"Nucleic acid sequence","level":3,"score":0.4549640119075775},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.4225609600543976},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3451992869377136},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.2092570662498474}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000953","descriptor_name":"Antigens, Protozoan","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004251","descriptor_name":"DNA Transposable Elements","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011995","descriptor_name":"Recombination, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012091","descriptor_name":"Repetitive Sequences, Nucleic Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016054","descriptor_name":"DNA, Protozoan","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D016805","descriptor_name":"Paramecium tetraurelia","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D016805","descriptor_name":"Paramecium tetraurelia","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":false},{"descriptor_ui":"D017354","descriptor_name":"Point Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1111/j.1550-7408.2004.tb00606.x","doi":"https://doi.org/10.1111/j.1550-7408.2004.tb00606.x","is_oa":false,"landing_page_url":"https://doi.org/10.1111/j.1550-7408.2004.tb00606.x","pdf_url":null,"source":{"id":"https://openalex.org/S73111969","display_name":"Journal of Eukaryotic Microbiology","issn_l":"1066-5234","issn":["1066-5234","1550-7408"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Eukaryotic Microbiology","raw_type":"journal-article"},{"id":"pmid:15666724","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/15666724","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of eukaryotic microbiology","raw_type":"Journal Article"},{"id":"pmh:10.1111/j.1550-7408.2004.tb00606.x","doi":null,"is_oa":false,"landing_page_url":"http://www.bioone.org/doi/10.1111/j.1550-7408.2004.tb00606.x","pdf_url":null,"source":{"id":"https://openalex.org/S4306402358","display_name":"BioOne Complete (BioOne)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210111627","host_organization_name":"BioOne","host_organization_lineage":["https://openalex.org/I4210111627"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"text"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":33,"referenced_works":["https://openalex.org/W994306213","https://openalex.org/W1549605620","https://openalex.org/W1602905276","https://openalex.org/W1850031504","https://openalex.org/W1912524364","https://openalex.org/W1946483892","https://openalex.org/W1965124661","https://openalex.org/W1974194748","https://openalex.org/W1979965913","https://openalex.org/W1984145694","https://openalex.org/W1992657064","https://openalex.org/W2002135879","https://openalex.org/W2003668302","https://openalex.org/W2033722092","https://openalex.org/W2041714273","https://openalex.org/W2052693103","https://openalex.org/W2052738936","https://openalex.org/W2071751082","https://openalex.org/W2073007371","https://openalex.org/W2073403450","https://openalex.org/W2096369882","https://openalex.org/W2104772148","https://openalex.org/W2109298048","https://openalex.org/W2123636562","https://openalex.org/W2128125656","https://openalex.org/W2128846574","https://openalex.org/W2133749577","https://openalex.org/W2139664841","https://openalex.org/W2144634347","https://openalex.org/W2144993001","https://openalex.org/W2167174680","https://openalex.org/W2804007336","https://openalex.org/W3139415968"],"related_works":["https://openalex.org/W2128846574","https://openalex.org/W1523310221","https://openalex.org/W1946483892","https://openalex.org/W2132629319","https://openalex.org/W2888950941","https://openalex.org/W2125980154","https://openalex.org/W2164512917","https://openalex.org/W2104772148","https://openalex.org/W2144993001","https://openalex.org/W2052584063"],"abstract_inverted_index":{"The":[0,101],"excision":[1,68,114,147],"of":[2,11,31,39,52,69,89,108,151,172,187],"internal":[3],"eliminated":[4],"sequences":[5],"(IESs)":[6],"occurs":[7],"during":[8],"the":[9,29,50,73,85,105,109,122,168],"differentiation":[10],"a":[12,82,94,158],"new":[13],"somatic":[14],"macronuclear":[15],"genome":[16],"in":[17,67,72,84,148],"ciliated":[18],"protozoa.":[19],"In":[20],"Paramecium":[21,135],"tetraurelia,":[22],"IESs":[23,71,153],"show":[24],"few":[25,142],"conserved":[26],"features":[27,186],"with":[28,47,133,184],"exception":[30],"an":[32,40],"invariant":[33],"5'-TA-3'":[34,87,110],"dinucleotide":[35,88],"that":[36,64,98,104,121,139,155],"is":[37,111,128,157],"part":[38],"8-bp":[41],"inverted":[42],"terminal":[43],"repeat":[44],"consensus":[45],"sequence":[46,97],"similarity":[48],"to":[49,178],"ends":[51],"mariner/Tc1":[53],"transposons.":[54],"We":[55],"have":[56,119],"isolated":[57],"and":[58,91],"analyzed":[59],"two":[60],"mutant":[61],"cell":[62,79],"lines":[63],"are":[65,141],"defective":[66],"individual":[70],"A-51":[74],"surface":[75],"antigen":[76],"gene.":[77],"Each":[78],"line":[80],"contains":[81],"mutation":[83],"flanking":[86,96],"IES6435":[90],"IES1835":[92],"creating":[93],"5'-CA-3'":[95],"prevents":[99],"excision.":[100,188],"results":[102,132],"demonstrate":[103],"first":[106],"position":[107,124],"required":[112],"IES":[113,136,146,169,180],"just":[115],"as":[116],"previous":[117],"mutants":[118,137],"shown":[120],"second":[123],"(the":[125],"A":[126],"residue)":[127],"required.":[129],"Combining":[130],"these":[131,173],"other":[134],"suggests":[138],"there":[140,156],"positions":[143,166,176],"essential":[144],"for":[145],"Paramecium.":[149],"Analysis":[150],"many":[152],"reveals":[154],"strong":[159],"bias":[160],"against":[161],"particular":[162],"nucleotides":[163],"at":[164],"some":[165],"near":[167],"termini.":[170],"Some":[171],"strongly":[174],"biased":[175],"correspond":[177],"known":[179],"mutations,":[181],"others":[182],"correlate":[183],"unusual":[185]},"counts_by_year":[{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}