{"id":"https://openalex.org/W2139072399","doi":"https://doi.org/10.1046/j.1365-313x.2000.00672.x","title":"Cloning and functional expression of the gene encoding the key enzyme for chlorophyll b biosynthesis (CAO) from Arabidopsis thaliana","display_name":"Cloning and functional expression of the gene encoding the key enzyme for chlorophyll b biosynthesis (CAO) from Arabidopsis thaliana","publication_year":2000,"publication_date":"2000-02-01","ids":{"openalex":"https://openalex.org/W2139072399","doi":"https://doi.org/10.1046/j.1365-313x.2000.00672.x","mag":"2139072399","pmid":"https://pubmed.ncbi.nlm.nih.gov/10758481"},"language":"en","primary_location":{"id":"doi:10.1046/j.1365-313x.2000.00672.x","doi":"https://doi.org/10.1046/j.1365-313x.2000.00672.x","is_oa":true,"landing_page_url":"https://doi.org/10.1046/j.1365-313x.2000.00672.x","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1046/j.1365-313x.2000.00672.x","source":{"id":"https://openalex.org/S67862205","display_name":"The Plant Journal","issn_l":"0960-7412","issn":["0960-7412","1365-313X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Plant Journal","raw_type":"journal-article"},"parent_works":[],"lineage":["https://openalex.org/W2139072399"],"child_works_count":0,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1046/j.1365-313x.2000.00672.x","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5021659865","display_name":"Ulrike Oster","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I205349734","display_name":"Hokkaido University","ror":"https://ror.org/02e16g702","country_code":"JP","type":"education","lineage":["https://openalex.org/I205349734"]},{"id":"https://openalex.org/I4405287530","display_name":"Institute of Low Temperature Science, Hokkaido University","ror":"https://ror.org/05edd0703","country_code":"JP","type":"facility","lineage":["https://openalex.org/I205349734","https://openalex.org/I4405287530"]},{"id":"https://openalex.org/I8204097","display_name":"Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen","ror":"https://ror.org/05591te55","country_code":"DE","type":"education","lineage":["https://openalex.org/I8204097"]}],"countries":["DE","JP"],"is_corresponding":false,"raw_author_name":"Ulrike Oster","raw_affiliation_strings":["Institute of Botany, University of Munich, Menzinger Str. 67, D-80638 Munich, Germany, andInstitute of Low Temperature Science, Hokkaido University, N19W8 Sapporo, Japan","Institute of Botany, University of Munich, Menzinger Str. 67, D\u201080638 Munich, Germany, and"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Botany, University of Munich, Menzinger Str. 67, D-80638 Munich, Germany, andInstitute of Low Temperature Science, Hokkaido University, N19W8 Sapporo, Japan","institution_ids":["https://openalex.org/I205349734","https://openalex.org/I4405287530","https://openalex.org/I8204097"]},{"raw_affiliation_string":"Institute of Botany, University of Munich, Menzinger Str. 67, D\u201080638 Munich, Germany, and","institution_ids":["https://openalex.org/I8204097"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5013151990","display_name":"Ryouichi Tanaka","orcid":"https://orcid.org/0000-0002-5858-5326","observed_orcids":["https://orcid.org/0000-0002-5858-5326"]},"institutions":[{"id":"https://openalex.org/I205349734","display_name":"Hokkaido University","ror":"https://ror.org/02e16g702","country_code":"JP","type":"education","lineage":["https://openalex.org/I205349734"]},{"id":"https://openalex.org/I4405287530","display_name":"Institute of Low Temperature Science, Hokkaido University","ror":"https://ror.org/05edd0703","country_code":"JP","type":"facility","lineage":["https://openalex.org/I205349734","https://openalex.org/I4405287530"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Ryouichi Tanaka","raw_affiliation_strings":["Institute of Low Temperature Science, Hokkaido University, N19W8 Sapporo, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Low Temperature Science, Hokkaido University, N19W8 Sapporo, Japan","institution_ids":["https://openalex.org/I205349734","https://openalex.org/I4405287530"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5103786719","display_name":"Ayumi Tanaka","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I205349734","display_name":"Hokkaido University","ror":"https://ror.org/02e16g702","country_code":"JP","type":"education","lineage":["https://openalex.org/I205349734"]},{"id":"https://openalex.org/I4405287530","display_name":"Institute of Low Temperature Science, Hokkaido University","ror":"https://ror.org/05edd0703","country_code":"JP","type":"facility","lineage":["https://openalex.org/I205349734","https://openalex.org/I4405287530"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Ayumi Tanaka","raw_affiliation_strings":["Institute of Low Temperature Science, Hokkaido University, N19W8 Sapporo, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Low Temperature Science, Hokkaido University, N19W8 Sapporo, Japan","institution_ids":["https://openalex.org/I205349734","https://openalex.org/I4405287530"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5014384203","display_name":"Wolfhart R\u00fcdiger","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I8204097","display_name":"Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen","ror":"https://ror.org/05591te55","country_code":"DE","type":"education","lineage":["https://openalex.org/I8204097"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"Wolfhart R\u00fcdiger","raw_affiliation_strings":["Institute of Botany, University of Munich, Menzinger Str. 67, D-80638 Munich, Germany, and"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Botany, University of Munich, Menzinger Str. 67, D-80638 Munich, Germany, and","institution_ids":["https://openalex.org/I8204097"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5014384203"],"corresponding_institution_ids":["https://openalex.org/I8204097"],"apc_list":{"value":5450,"currency":"USD","value_usd":5450},"apc_paid":null,"fwci":2.4883,"has_fulltext":true,"cited_by_count":274,"citation_normalized_percentile":{"value":0.8991158,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":97,"max":100},"biblio":{"volume":"21","issue":"3","first_page":"305","last_page":"310"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10303","display_name":"Photosynthetic Processes and Mechanisms","score":0.49880000948905945,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10303","display_name":"Photosynthetic Processes and Mechanisms","score":0.49880000948905945,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11882","display_name":"Plant biochemistry and biosynthesis","score":0.3955000042915344,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12441","display_name":"Porphyrin Metabolism and Disorders","score":0.03269999846816063,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/chlorophyllide-a-oxygenase","display_name":"chlorophyllide a oxygenase","score":0.9940000176429749},{"id":"https://openalex.org/keywords/arabidopsis-thaliana","display_name":"Arabidopsis thaliana","score":0.9679999947547913},{"id":"https://openalex.org/keywords/recombinant-enzyme-production","display_name":"recombinant enzyme expression","score":0.8550000190734863},{"id":"https://openalex.org/keywords/light-harvesting-complex","display_name":"light-harvesting complexes","score":0.8169999718666077},{"id":"https://openalex.org/keywords/chlorophyll-degradation","display_name":"chlorophyll degradation","score":0.734000027179718}],"concepts":[{"id":"https://openalex.org/C2775988993","wikidata":"https://www.wikidata.org/wiki/Q158695","display_name":"Arabidopsis thaliana","level":4,"score":0.744855523109436},{"id":"https://openalex.org/C553450214","wikidata":"https://www.wikidata.org/wiki/Q851162","display_name":"Biosynthesis","level":3,"score":0.6710315942764282},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.5988733768463135},{"id":"https://openalex.org/C165474305","wikidata":"https://www.wikidata.org/wiki/Q419615","display_name":"Oxygenase","level":3,"score":0.5942792892456055},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.57924884557724},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.5668216347694397},{"id":"https://openalex.org/C2776373379","wikidata":"https://www.wikidata.org/wiki/Q43177","display_name":"Chlorophyll","level":2,"score":0.5205181837081909},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4860287308692932},{"id":"https://openalex.org/C121050878","wikidata":"https://www.wikidata.org/wiki/Q5135020","display_name":"Cloning (programming)","level":2,"score":0.47990238666534424},{"id":"https://openalex.org/C2778100927","wikidata":"https://www.wikidata.org/wiki/Q1943387","display_name":"Chlorophyll b","level":3,"score":0.4474482834339142},{"id":"https://openalex.org/C183688256","wikidata":"https://www.wikidata.org/wiki/Q11982","display_name":"Photosynthesis","level":2,"score":0.42947423458099365},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.4049934148788452},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3427693843841553},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.2739778757095337},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.2507357597351074},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.0},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D002734","descriptor_name":"Chlorophyll","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010105","descriptor_name":"Oxygenases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D010105","descriptor_name":"Oxygenases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D017360","descriptor_name":"Arabidopsis","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D017360","descriptor_name":"Arabidopsis","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1046/j.1365-313x.2000.00672.x","doi":"https://doi.org/10.1046/j.1365-313x.2000.00672.x","is_oa":true,"landing_page_url":"https://doi.org/10.1046/j.1365-313x.2000.00672.x","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1046/j.1365-313x.2000.00672.x","source":{"id":"https://openalex.org/S67862205","display_name":"The Plant Journal","issn_l":"0960-7412","issn":["0960-7412","1365-313X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Plant Journal","raw_type":"journal-article"},{"id":"pmid:10758481","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10758481","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Plant journal : for cell and molecular biology","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1046/j.1365-313x.2000.00672.x","doi":"https://doi.org/10.1046/j.1365-313x.2000.00672.x","is_oa":true,"landing_page_url":"https://doi.org/10.1046/j.1365-313x.2000.00672.x","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1046/j.1365-313x.2000.00672.x","source":{"id":"https://openalex.org/S67862205","display_name":"The Plant Journal","issn_l":"0960-7412","issn":["0960-7412","1365-313X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-a","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Plant Journal","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320320879","display_name":"Deutsche Forschungsgemeinschaft","ror":"https://ror.org/018mejw64"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2139072399.pdf","grobid_xml":"https://content.openalex.org/works/W2139072399.grobid-xml"},"referenced_works_count":24,"referenced_works":["https://openalex.org/W1516369138","https://openalex.org/W1522645863","https://openalex.org/W1595595467","https://openalex.org/W1639022828","https://openalex.org/W1947530394","https://openalex.org/W1970912353","https://openalex.org/W1973586221","https://openalex.org/W1976083655","https://openalex.org/W1988335372","https://openalex.org/W1990478672","https://openalex.org/W2008742882","https://openalex.org/W2014151429","https://openalex.org/W2016109833","https://openalex.org/W2033247319","https://openalex.org/W2038202034","https://openalex.org/W2052606001","https://openalex.org/W2069993933","https://openalex.org/W2093688162","https://openalex.org/W2118472374","https://openalex.org/W2139093556","https://openalex.org/W2149545078","https://openalex.org/W2172173039","https://openalex.org/W2276757361","https://openalex.org/W2951898453"],"related_works":["https://openalex.org/W2093688162","https://openalex.org/W196531186","https://openalex.org/W7095772102","https://openalex.org/W7133610365","https://openalex.org/W2078784731","https://openalex.org/W2120793765","https://openalex.org/W7166034635","https://openalex.org/W4389283547"],"abstract_inverted_index":{"Chlorophyll":[0],"(Chl)":[1],"biosynthesis":[2,43],"and":[3,63],"degradation":[4],"are":[5],"the":[6,25,30,42,49,61,66,78,100,104],"only":[7],"biochemical":[8],"processes":[9],"on":[10],"Earth":[11],"that":[12,98],"can":[13],"be":[14],"directly":[15],"observed":[16],"from":[17,72],"satellites":[18],"or":[19],"other":[20],"planets.":[21],"The":[22,85],"bulk":[23],"of":[24,34,44,65,107],"Chls":[26],"is":[27,39,48,76,99],"found":[28],"in":[29,81,89,103],"light-harvesting":[31],"antenna":[32],"complexes":[33],"photosynthetic":[35],"organisms.":[36],"Surprisingly":[37],"little":[38],"known":[40],"about":[41],"Chl":[45,53,56,82],"b,":[46],"which":[47,75],"second":[50],"most":[51],"abundant":[52],"pigment":[54],"after":[55],"a.":[57],"We":[58],"describe":[59],"here":[60],"expression":[62],"properties":[64],"chlorophyllide":[67],"a":[68],"oxygenase":[69,96],"gene":[70],"(CAO)":[71],"Arabidopsis":[73],"thaliana,":[74],"apparently":[77],"key":[79],"enzyme":[80,87],"b":[83],"biosynthesis.":[84],"recombinant":[86],"produced":[88],"Escherichia":[90],"coli":[91],"catalyses":[92],"an":[93],"unusual":[94],"two-step":[95],"reaction":[97],"'missing":[101],"link'":[102],"chlorophyll":[105],"cycle":[106],"higher":[108],"plants.":[109]},"counts_by_year":[{"year":2026,"cited_by_count":6},{"year":2025,"cited_by_count":8},{"year":2024,"cited_by_count":9},{"year":2023,"cited_by_count":12},{"year":2022,"cited_by_count":15},{"year":2021,"cited_by_count":14},{"year":2020,"cited_by_count":19},{"year":2019,"cited_by_count":12},{"year":2018,"cited_by_count":7},{"year":2017,"cited_by_count":8},{"year":2016,"cited_by_count":15},{"year":2015,"cited_by_count":5},{"year":2014,"cited_by_count":12},{"year":2013,"cited_by_count":15},{"year":2012,"cited_by_count":12}],"updated_date":"2026-11-14T11:04:58.165337","created_date":"2016-06-24T00:00:00"}