{"id":"https://openalex.org/W2138806571","doi":"https://doi.org/10.1186/1471-2105-15-284","title":"MT-Toolbox: improved amplicon sequencing using molecule tags","display_name":"MT-Toolbox: improved amplicon sequencing using molecule tags","publication_year":2014,"publication_date":"2014-08-22","ids":{"openalex":"https://openalex.org/W2138806571","doi":"https://doi.org/10.1186/1471-2105-15-284","mag":"2138806571","pmid":"https://pubmed.ncbi.nlm.nih.gov/25149069","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/4153912"},"language":"en","primary_location":{"id":"doi:10.1186/1471-2105-15-284","doi":"https://doi.org/10.1186/1471-2105-15-284","is_oa":true,"landing_page_url":"https://doi.org/10.1186/1471-2105-15-284","pdf_url":"https://bmcbioinformatics.biomedcentral.com/counter/pdf/10.1186/1471-2105-15-284","source":{"id":"https://openalex.org/S19032547","display_name":"BMC Bioinformatics","issn_l":"1471-2105","issn":["1471-2105"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","ccf-c","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-2","oarl","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Bioinformatics","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","datacite","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://bmcbioinformatics.biomedcentral.com/counter/pdf/10.1186/1471-2105-15-284","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5028275784","display_name":"Scott Yourstone","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I114027177","display_name":"University of North Carolina at Chapel Hill","ror":"https://ror.org/0130frc33","country_code":"US","type":"education","lineage":["https://openalex.org/I114027177"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Scott M Yourstone","raw_affiliation_strings":["Curriculum in Bioinformatics and Computational Biology, University of North Carolina, Chapel Hill 27599, USA. scott.yourstone81@gmail.com","Curriculum in Bioinformatics and Computational Biology, University of North Carolina, Chapel Hill, 27599, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Curriculum in Bioinformatics and Computational Biology, University of North Carolina, Chapel Hill 27599, USA. scott.yourstone81@gmail.com","institution_ids":["https://openalex.org/I114027177"]},{"raw_affiliation_string":"Curriculum in Bioinformatics and Computational Biology, University of North Carolina, Chapel Hill, 27599, USA","institution_ids":["https://openalex.org/I114027177"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068206920","display_name":"Derek S. Lundberg","orcid":"https://orcid.org/0000-0001-7970-1595","observed_orcids":["https://orcid.org/0000-0001-7970-1595"]},"institutions":[{"id":"https://openalex.org/I114027177","display_name":"University of North Carolina at Chapel Hill","ror":"https://ror.org/0130frc33","country_code":"US","type":"education","lineage":["https://openalex.org/I114027177"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Derek S Lundberg","raw_affiliation_strings":["Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, 27599, USA","Department of Biology, University of North Carolina, Chapel Hill, 27599, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, 27599, USA","institution_ids":["https://openalex.org/I114027177"]},{"raw_affiliation_string":"Department of Biology, University of North Carolina, Chapel Hill, 27599, USA","institution_ids":["https://openalex.org/I114027177"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5082498150","display_name":"Jeffery L. Dangl","orcid":"https://orcid.org/0000-0003-3199-8654","observed_orcids":["https://orcid.org/0000-0003-3199-8654"]},"institutions":[{"id":"https://openalex.org/I114027177","display_name":"University of North Carolina at Chapel Hill","ror":"https://ror.org/0130frc33","country_code":"US","type":"education","lineage":["https://openalex.org/I114027177"]},{"id":"https://openalex.org/I1344073410","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90","country_code":"US","type":"facility","lineage":["https://openalex.org/I1344073410"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jeffery L Dangl","raw_affiliation_strings":["Carolina Center for Genomic Sciences, University of North Carolina, Chapel Hill, 27599, USA","Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, 27599, USA","Department of Biology, University of North Carolina, Chapel Hill, 27599, USA","Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, 27599, USA","Howard Hughes Medical Institute, University of North Carolina, 400 Jones Bridge Road, Chevy Chase, MD, 20815-6789, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Carolina Center for Genomic Sciences, University of North Carolina, Chapel Hill, 27599, USA","institution_ids":["https://openalex.org/I114027177"]},{"raw_affiliation_string":"Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, 27599, USA","institution_ids":["https://openalex.org/I114027177"]},{"raw_affiliation_string":"Department of Biology, University of North Carolina, Chapel Hill, 27599, USA","institution_ids":["https://openalex.org/I114027177"]},{"raw_affiliation_string":"Department of Microbiology and Immunology, University of North Carolina, Chapel Hill, 27599, USA","institution_ids":["https://openalex.org/I114027177"]},{"raw_affiliation_string":"Howard Hughes Medical Institute, University of North Carolina, 400 Jones Bridge Road, Chevy Chase, MD, 20815-6789, USA","institution_ids":["https://openalex.org/I1344073410"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5091112024","display_name":"Corbin D. Jones","orcid":"https://orcid.org/0000-0001-7281-7130","observed_orcids":["https://orcid.org/0000-0001-7281-7130"]},"institutions":[{"id":"https://openalex.org/I114027177","display_name":"University of North Carolina at Chapel Hill","ror":"https://ror.org/0130frc33","country_code":"US","type":"education","lineage":["https://openalex.org/I114027177"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Corbin D Jones","raw_affiliation_strings":["Carolina Center for Genomic Sciences, University of North Carolina, Chapel Hill, 27599, USA","Curriculum in Bioinformatics and Computational Biology, University of North Carolina, Chapel Hill, 27599, USA","Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, 27599, USA","Department of Biology, University of North Carolina, Chapel Hill, 27599, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Carolina Center for Genomic Sciences, University of North Carolina, Chapel Hill, 27599, USA","institution_ids":["https://openalex.org/I114027177"]},{"raw_affiliation_string":"Curriculum in Bioinformatics and Computational Biology, University of North Carolina, Chapel Hill, 27599, USA","institution_ids":["https://openalex.org/I114027177"]},{"raw_affiliation_string":"Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, 27599, USA","institution_ids":["https://openalex.org/I114027177"]},{"raw_affiliation_string":"Department of Biology, University of North Carolina, Chapel Hill, 27599, USA","institution_ids":["https://openalex.org/I114027177"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5028275784"],"corresponding_institution_ids":["https://openalex.org/I114027177"],"apc_list":{"value":2990,"currency":"USD","value_usd":2990},"apc_paid":{"value":2990,"currency":"USD","value_usd":2990},"fwci":0.7894,"has_fulltext":true,"cited_by_count":25,"citation_normalized_percentile":{"value":0.68742816,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":97},"biblio":{"volume":"15","issue":"1","first_page":"284","last_page":"284"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.9761000275611877,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.9761000275611877,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12640","display_name":"Environmental DNA in Biodiversity Studies","score":0.02019999921321869,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11791","display_name":"Microbial Community Ecology and Physiology","score":0.0012000000569969416,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/consensus-building","display_name":"consensus building","score":0.8119999766349792},{"id":"https://openalex.org/keywords/16s-rrna","display_name":"16S rRNA","score":0.675000011920929},{"id":"https://openalex.org/keywords/metagenomics","display_name":"metagenomics","score":0.7990000247955322},{"id":"https://openalex.org/keywords/operational-taxonomic-units","display_name":"operational taxonomic units","score":0.9509999752044678},{"id":"https://openalex.org/keywords/pollutant-removal","display_name":"pollutant removal","score":0.8320000171661377},{"id":"https://openalex.org/keywords/taxonomic-assignment","display_name":"taxonomic assignment","score":0.7110000252723694},{"id":"https://openalex.org/keywords/barcoding","display_name":"barcoding","score":0.4180000126361847}],"concepts":[{"id":"https://openalex.org/C8185291","wikidata":"https://www.wikidata.org/wiki/Q2844457","display_name":"Amplicon","level":4,"score":0.7795289158821106},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.6657590866088867},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5986768007278442},{"id":"https://openalex.org/C129312508","wikidata":"https://www.wikidata.org/wiki/Q133686","display_name":"Oligonucleotide","level":3,"score":0.5648346543312073},{"id":"https://openalex.org/C2777655017","wikidata":"https://www.wikidata.org/wiki/Q1501161","display_name":"Toolbox","level":2,"score":0.4899079203605652},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2871398329734802},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.2294434905052185},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.2210889458656311},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.19604533910751343},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.16694766283035278},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D003196","descriptor_name":"Computer Graphics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004247","descriptor_name":"DNA","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D009841","descriptor_name":"Oligonucleotides","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012984","descriptor_name":"Software","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D013997","descriptor_name":"Time Factors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014584","descriptor_name":"User-Computer Interface","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D056186","descriptor_name":"Metagenomics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":7,"locations":[{"id":"doi:10.1186/1471-2105-15-284","doi":"https://doi.org/10.1186/1471-2105-15-284","is_oa":true,"landing_page_url":"https://doi.org/10.1186/1471-2105-15-284","pdf_url":"https://bmcbioinformatics.biomedcentral.com/counter/pdf/10.1186/1471-2105-15-284","source":{"id":"https://openalex.org/S19032547","display_name":"BMC Bioinformatics","issn_l":"1471-2105","issn":["1471-2105"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","ccf-c","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-2","oarl","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Bioinformatics","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:3124382","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4153912","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:4153912","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4153912","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Bioinformatics","raw_type":"Text"},{"id":"pmid:25149069","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/25149069","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC bioinformatics","raw_type":"Journal Article"},{"id":"pmh:oai:cdr.lib.unc.edu:uuid:f7a99180-eab0-4f41-b71d-bd3ce9288cf4","doi":null,"is_oa":true,"landing_page_url":"https://cdr.lib.unc.edu/record/uuid:f7a99180-eab0-4f41-b71d-bd3ce9288cf4","pdf_url":null,"source":{"id":"https://openalex.org/S4306401075","display_name":"Carolina Digital Repository (University of North Carolina at Chapel Hill)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I114027177","host_organization_name":"University of North Carolina at Chapel Hill","host_organization_lineage":["https://openalex.org/I114027177"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Text"},{"id":"pmh:cdr.lib.unc.edu:zk51vk360","doi":null,"is_oa":false,"landing_page_url":"https://doi.org/10.17615/00td-b183","pdf_url":null,"source":{"id":"https://openalex.org/S4306401075","display_name":"Carolina Digital Repository (University of North Carolina at Chapel Hill)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I114027177","host_organization_name":"University of North Carolina at Chapel Hill","host_organization_lineage":["https://openalex.org/I114027177"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"BMC Bioinformatics, 15(1)","raw_type":"Article"},{"id":"doi:10.17615/00td-b183","doi":"https://doi.org/10.17615/00td-b183","is_oa":true,"landing_page_url":"https://doi.org/10.17615/00td-b183","pdf_url":null,"source":{"id":"https://openalex.org/S7407051488","display_name":"UNC Libraries","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"article-journal"}],"best_oa_location":{"id":"doi:10.1186/1471-2105-15-284","doi":"https://doi.org/10.1186/1471-2105-15-284","is_oa":true,"landing_page_url":"https://doi.org/10.1186/1471-2105-15-284","pdf_url":"https://bmcbioinformatics.biomedcentral.com/counter/pdf/10.1186/1471-2105-15-284","source":{"id":"https://openalex.org/S19032547","display_name":"BMC Bioinformatics","issn_l":"1471-2105","issn":["1471-2105"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-class-a","anvur-scientific","ccf-c","cwts-core","doaj","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-2","oarl","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320256","host_organization_name":"BioMed Central","host_organization_lineage":["https://openalex.org/P4310320256","https://openalex.org/P4310319965"],"host_organization_lineage_names":["BioMed Central","Springer Nature"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BMC Bioinformatics","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G1395621007","display_name":"Collaborative Research:  MSB: Defining Plant-Associated Metagenomics","funder_award_id":"0958245","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"},{"id":"https://openalex.org/G3333285807","display_name":null,"funder_award_id":"2013-MRG-1110","funder_id":"https://openalex.org/F4320308571","funder_display_name":"North Carolina Biotechnology Center"},{"id":"https://openalex.org/G4163747806","display_name":"Predoctoral Training Program in Bioinformatics and Computational Biology","funder_award_id":"T32GM067553","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"},{"id":"https://openalex.org/F4320306082","display_name":"Howard Hughes Medical Institute","ror":"https://ror.org/006w34k90"},{"id":"https://openalex.org/F4320306202","display_name":"Gordon and Betty Moore Foundation","ror":"https://ror.org/006wxqw41"},{"id":"https://openalex.org/F4320308571","display_name":"North Carolina Biotechnology Center","ror":"https://ror.org/03hj4jr80"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2138806571.pdf","grobid_xml":"https://content.openalex.org/works/W2138806571.grobid-xml"},"referenced_works_count":18,"referenced_works":["https://openalex.org/W987420839","https://openalex.org/W1912338927","https://openalex.org/W1967178396","https://openalex.org/W2031550784","https://openalex.org/W2041677964","https://openalex.org/W2072970694","https://openalex.org/W2119278037","https://openalex.org/W2124351063","https://openalex.org/W2132926880","https://openalex.org/W2133385595","https://openalex.org/W2137015675","https://openalex.org/W2137195441","https://openalex.org/W2142678478","https://openalex.org/W2152241467","https://openalex.org/W2152885278","https://openalex.org/W2153284232","https://openalex.org/W2157107905","https://openalex.org/W2163576465"],"related_works":["https://openalex.org/W2346268682","https://openalex.org/W2031550784","https://openalex.org/W25894764","https://openalex.org/W2148460201","https://openalex.org/W2521580904","https://openalex.org/W2150228362","https://openalex.org/W3120817351","https://openalex.org/W2398851688","https://openalex.org/W3121089444","https://openalex.org/W4407047676"],"abstract_inverted_index":{"BACKGROUND:":[0],"Short":[1],"oligonucleotides":[2,25],"can":[3,74,192],"be":[4,75,193],"used":[5],"as":[6,225],"markers":[7],"to":[8,26,51,66,78],"tag":[9,93],"and":[10,106,151,184,188,234,254,263],"track":[11],"DNA":[12,32,53],"sequences.":[13],"For":[14],"example,":[15],"barcoding":[16],"techniques":[17],"(i.e.":[18],"Multiplex":[19],"Identifiers":[20],"or":[21,136,202],"Indexing)":[22],"use":[23],"short":[24],"distinguish":[27],"between":[28],"reads":[29,131,249],"from":[30,88,128],"different":[31],"samples":[33],"pooled":[34],"for":[35,118,124,140,186,218,244,265],"high-throughput":[36],"sequencing.":[37],"A":[38],"similar":[39,116],"technique":[40],"called":[41],"molecule":[42,58,119,129,161,247],"tagging":[43],"uses":[44],"the":[45,91,153],"same":[46,92],"principles":[47],"but":[48],"is":[49,59,132,137],"applied":[50],"individual":[52],"template":[54,57,98,155],"molecules.":[55],"Each":[56],"tagged":[60,130,162,248],"with":[61],"a":[62,141,158,177,198,206],"unique":[63],"oligonucleotide":[64,84,147],"prior":[65],"polymerase":[67,107,255],"chain":[68,108,256],"reaction.":[69],"The":[70],"resulting":[71],"amplicon":[72,270],"sequences":[73,89,127,167],"traced":[76],"back":[77],"their":[79,83],"original":[80,97],"templates":[81],"by":[82],"tag.":[85],"Consensus":[86],"building":[87,228],"sharing":[90],"enables":[94],"inference":[95],"of":[96,103,160,246],"molecules":[99],"thereby":[100,250],"reducing":[101,251],"effects":[102],"sequencing":[104,271],"error":[105],"reaction":[109,257],"bias.":[110,258],"Several":[111],"independent":[112],"groups":[113],"have":[114],"developed":[115],"protocols":[117],"tagging;":[120],"however,":[121],"user-friendly":[122,242],"software":[123],"build":[125],"consensus":[126],"not":[133],"readily":[134],"available":[135],"highly":[138],"specific":[139],"particular":[142],"protocol.":[143],"RESULTS:":[144],"MT-Toolbox":[145,165,175,238],"recognizes":[146],"tags":[148],"in":[149,195,203,268],"amplicons":[150],"infers":[152],"correct":[154],"sequence.":[156],"On":[157],"set":[159],"test":[163],"reads,":[164],"generates":[166],"having":[168],"on":[169,197,205],"average":[170],"0.00047":[171],"errors":[172,253],"per":[173],"base.":[174],"includes":[176],"graphical":[178],"user":[179],"interface,":[180,183],"command":[181],"line":[182],"options":[185],"speed":[187],"accuracy":[189],"maximization.":[190],"It":[191],"run":[194],"serial":[196],"standard":[199],"personal":[200],"computer":[201],"parallel":[204],"Load":[207],"Sharing":[208],"Facility":[209],"based":[210],"cluster":[211],"system.":[212],"An":[213],"optional":[214],"plugin":[215],"provides":[216,239],"features":[217],"common":[219],"16S":[220],"metagenome":[221],"profiling":[222],"analysis":[223,245],"such":[224],"chimera":[226],"filtering,":[227],"operational":[229],"taxonomic":[230],"units,":[231],"contaminant":[232],"removal,":[233],"taxonomy":[235],"assignments.":[236],"CONCLUSIONS:":[237],"an":[240],"accessible,":[241],"environment":[243],"technical":[252],"These":[259],"improvements":[260],"reduce":[261],"noise":[262],"allow":[264],"greater":[266],"precision":[267],"single":[269],"experiments.":[272]},"counts_by_year":[{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":3},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":4},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":3},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":1}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2016-06-24T00:00:00"}