{"id":"https://openalex.org/W2138573199","doi":"https://doi.org/10.1099/00221287-143-10-3295","title":"Characterization of the rate-limiting step of the secretion of Bacillus subtilis \u03b1-amylase overproduced during the exponential phase of growth","display_name":"Characterization of the rate-limiting step of the secretion of Bacillus subtilis \u03b1-amylase overproduced during the exponential phase of growth","publication_year":1997,"publication_date":"1997-10-01","ids":{"openalex":"https://openalex.org/W2138573199","doi":"https://doi.org/10.1099/00221287-143-10-3295","mag":"2138573199","pmid":"https://pubmed.ncbi.nlm.nih.gov/9353930"},"language":"en","primary_location":{"id":"doi:10.1099/00221287-143-10-3295","doi":"https://doi.org/10.1099/00221287-143-10-3295","is_oa":false,"landing_page_url":"https://doi.org/10.1099/00221287-143-10-3295","pdf_url":null,"source":{"id":"https://openalex.org/S196799759","display_name":"Microbiology","issn_l":"1350-0872","issn":["1350-0872","1465-2080"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320497","host_organization_name":"Microbiology Society","host_organization_lineage":["https://openalex.org/P4310320497"],"host_organization_lineage_names":["Microbiology Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microbiology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5024695687","display_name":"Laurence Leloup","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I4210113761","display_name":"Institut Jacques Monod","ror":"https://ror.org/02c5gc203","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I154526488","https://openalex.org/I204730241","https://openalex.org/I4210096427","https://openalex.org/I4210113761"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Laurence Leloup","raw_affiliation_strings":["Institut Jacques Monod, CNRS, Universit\u00e9 Paris 7 Denis Diderot, Laboratoire G\u00e9n\u00e9tique et Membranes, Tour 43-2, place Jussieu, 75251 Paris Cedex 05, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institut Jacques Monod, CNRS, Universit\u00e9 Paris 7 Denis Diderot, Laboratoire G\u00e9n\u00e9tique et Membranes, Tour 43-2, place Jussieu, 75251 Paris Cedex 05, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I4210113761"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5057023295","display_name":"Elarbi Haddaoui","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I4210113761","display_name":"Institut Jacques Monod","ror":"https://ror.org/02c5gc203","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I154526488","https://openalex.org/I204730241","https://openalex.org/I4210096427","https://openalex.org/I4210113761"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"El Arbi Haddaoui","raw_affiliation_strings":["Institut Jacques Monod, CNRS, Universit\u00e9 Paris 7 Denis Diderot, Laboratoire G\u00e9n\u00e9tique et Membranes, Tour 43-2, place Jussieu, 75251 Paris Cedex 05, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institut Jacques Monod, CNRS, Universit\u00e9 Paris 7 Denis Diderot, Laboratoire G\u00e9n\u00e9tique et Membranes, Tour 43-2, place Jussieu, 75251 Paris Cedex 05, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I4210113761"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5039114163","display_name":"R\u00e9gis Chambert","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]},{"id":"https://openalex.org/I4210113761","display_name":"Institut Jacques Monod","ror":"https://ror.org/02c5gc203","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I154526488","https://openalex.org/I204730241","https://openalex.org/I4210096427","https://openalex.org/I4210113761"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"R\u00e9gis Chambert","raw_affiliation_strings":["Institut Jacques Monod, CNRS, Universit\u00e9 Paris 7 Denis Diderot, Laboratoire G\u00e9n\u00e9tique et Membranes, Tour 43-2, place Jussieu, 75251 Paris Cedex 05, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institut Jacques Monod, CNRS, Universit\u00e9 Paris 7 Denis Diderot, Laboratoire G\u00e9n\u00e9tique et Membranes, Tour 43-2, place Jussieu, 75251 Paris Cedex 05, France","institution_ids":["https://openalex.org/I1294671590","https://openalex.org/I4210113761"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5110148135","display_name":"Marie\u2010Fran\u00e7oise Petit\u2010Glatron","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1294671590","display_name":"Centre National de la Recherche Scientifique","ror":"https://ror.org/02feahw73","country_code":"FR","type":"government","lineage":["https://openalex.org/I1294671590"]}],"countries":["FR"],"is_corresponding":true,"raw_author_name":"Marie-Fran\u00c7oise Petit-Glatron","raw_affiliation_strings":["Denis Diderot, Laboratoire GBnetique et Membranes,","CNRS, Universite Paris"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Denis Diderot, Laboratoire GBnetique et Membranes,","institution_ids":[]},{"raw_affiliation_string":"CNRS, Universite Paris","institution_ids":["https://openalex.org/I1294671590"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5110148135"],"corresponding_institution_ids":["https://openalex.org/I1294671590"],"apc_list":null,"apc_paid":null,"fwci":2.0803,"has_fulltext":false,"cited_by_count":56,"citation_normalized_percentile":{"value":0.86910025,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":95},"biblio":{"volume":"143","issue":"10","first_page":"3295","last_page":"3303"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9028000235557556,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9028000235557556,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.06599999964237213,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.021400000900030136,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/bacillus-subtilis-alpha-amylase","display_name":"Bacillus subtilis alpha-amylase","score":0.9359999895095825},{"id":"https://openalex.org/keywords/protein-secretion","display_name":"protein secretion","score":0.6940000057220459},{"id":"https://openalex.org/keywords/rate-limiting-step","display_name":"rate-determining step","score":0.8889999985694885},{"id":"https://openalex.org/keywords/signal-peptide-cleavage","display_name":"signal peptide cleavage","score":0.8669999837875366},{"id":"https://openalex.org/keywords/cell-wall","display_name":"cell wall","score":0.40700000524520874},{"id":"https://openalex.org/keywords/protein-folding","display_name":"protein folding","score":0.7570000290870667},{"id":"https://openalex.org/keywords/calcium-requirement","display_name":"calcium requirement","score":0.699999988079071},{"id":"https://openalex.org/keywords/edta","display_name":"EDTA","score":0.7630000114440918},{"id":"https://openalex.org/keywords/protoplasts","display_name":"protoplasts","score":0.859000027179718},{"id":"https://openalex.org/keywords/levansucrase","display_name":"levansucrase","score":0.8389999866485596},{"id":"https://openalex.org/keywords/sucrose-induction","display_name":"sucrose induction","score":0.753000020980835},{"id":"https://openalex.org/keywords/pulse-chase","display_name":"pulse-chase","score":0.6549999713897705},{"id":"https://openalex.org/keywords/in-vitro-folding","display_name":"in vitro folding","score":0.4320000112056732}],"concepts":[{"id":"https://openalex.org/C2777272437","wikidata":"https://www.wikidata.org/wiki/Q131238","display_name":"Bacillus subtilis","level":3,"score":0.788651704788208},{"id":"https://openalex.org/C10858879","wikidata":"https://www.wikidata.org/wiki/Q899790","display_name":"Signal peptide","level":4,"score":0.5836926102638245},{"id":"https://openalex.org/C170835558","wikidata":"https://www.wikidata.org/wiki/Q17153","display_name":"Amylase","level":3,"score":0.5198079347610474},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5155463218688965},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.5023090839385986},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4815797805786133},{"id":"https://openalex.org/C49039625","wikidata":"https://www.wikidata.org/wiki/Q84230","display_name":"Secretion","level":2,"score":0.48154905438423157},{"id":"https://openalex.org/C2780349071","wikidata":"https://www.wikidata.org/wiki/Q30836","display_name":"Levansucrase","level":4,"score":0.472700834274292},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3793667256832123},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3306056261062622},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.29464706778526306},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.1879425048828125},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.14828020334243774},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.11301305890083313}],"mesh":[{"descriptor_ui":"D000516","descriptor_name":"alpha-Amylases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000516","descriptor_name":"alpha-Amylases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D000516","descriptor_name":"alpha-Amylases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001412","descriptor_name":"Bacillus subtilis","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D001412","descriptor_name":"Bacillus subtilis","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001412","descriptor_name":"Bacillus subtilis","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":false},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002473","descriptor_name":"Cell Wall","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D002473","descriptor_name":"Cell Wall","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D002614","descriptor_name":"Chelating Agents","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D004492","descriptor_name":"Edetic Acid","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D005798","descriptor_name":"Genes, Bacterial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011523","descriptor_name":"Protoplasts","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D013395","descriptor_name":"Sucrose","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D015964","descriptor_name":"Gene Expression Regulation, Bacterial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017510","descriptor_name":"Protein Folding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017931","descriptor_name":"DNA Primers","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D019677","descriptor_name":"Artificial Gene Fusion","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D021382","descriptor_name":"Protein Sorting Signals","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1099/00221287-143-10-3295","doi":"https://doi.org/10.1099/00221287-143-10-3295","is_oa":false,"landing_page_url":"https://doi.org/10.1099/00221287-143-10-3295","pdf_url":null,"source":{"id":"https://openalex.org/S196799759","display_name":"Microbiology","issn_l":"1350-0872","issn":["1350-0872","1465-2080"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320497","host_organization_name":"Microbiology Society","host_organization_lineage":["https://openalex.org/P4310320497"],"host_organization_lineage_names":["Microbiology Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microbiology","raw_type":"journal-article"},{"id":"pmid:9353930","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9353930","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microbiology (Reading, England)","raw_type":"Journal Article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.318.2108","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.318.2108","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://mic.sgmjournals.org/content/143/10/3295.full.pdf","raw_type":"text"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":29,"referenced_works":["https://openalex.org/W157779417","https://openalex.org/W1495560120","https://openalex.org/W1536594497","https://openalex.org/W1539852154","https://openalex.org/W1552311473","https://openalex.org/W1617456996","https://openalex.org/W1671375346","https://openalex.org/W1872409191","https://openalex.org/W1907206755","https://openalex.org/W1910958380","https://openalex.org/W1923943875","https://openalex.org/W1924556801","https://openalex.org/W1969301105","https://openalex.org/W1978117360","https://openalex.org/W1999199911","https://openalex.org/W2021130823","https://openalex.org/W2029118537","https://openalex.org/W2034588339","https://openalex.org/W2059718816","https://openalex.org/W2063232972","https://openalex.org/W2065288048","https://openalex.org/W2075354188","https://openalex.org/W2114153386","https://openalex.org/W2121923416","https://openalex.org/W2124885409","https://openalex.org/W2151944333","https://openalex.org/W2159353958","https://openalex.org/W2170422080","https://openalex.org/W4234154879"],"related_works":["https://openalex.org/W2087630987","https://openalex.org/W7095651710","https://openalex.org/W2738158054","https://openalex.org/W429982799","https://openalex.org/W2010551277","https://openalex.org/W1989283983","https://openalex.org/W2100939492","https://openalex.org/W104874150","https://openalex.org/W7097242779","https://openalex.org/W7017514403"],"abstract_inverted_index":{"The":[0,18,105,131,158,200],"Bacillus":[1],"subtilis":[2],"alpha-amylase":[3,51,144,204],"gene,":[4],"amyE,":[5],"was":[6,32,151,167],"expressed":[7],"under":[8],"the":[9,14,22,28,35,43,55,67,80,88,92,116,125,140,147,163,170,180,194,197,203,222,225,245],"regulated":[10],"control":[11],"of":[12,37,42,63,70,87,94,107,111,118,143,182,196,202,210,244],"sacR,":[13],"levansucrase":[15],"leader":[16],"region.":[17],"gene":[19],"fusion":[20],"including":[21],"complete":[23],"amyE":[24],"coding":[25],"sequence":[26,31],"with":[27,139],"signal":[29,100],"peptide":[30,101],"integrated":[33],"into":[34],"chromosome":[36],"a":[38,59,95,119],"degU32(Hy)":[39],"strain":[40],"deleted":[41],"sacB":[44],"DNA":[45],"fragment.":[46],"In":[47],"this":[48,108,134],"genetic":[49],"contex,":[50],"is":[52,91,136,207],"produced":[53],"in":[54,115,154,191],"culture":[56],"supernatant":[57],"at":[58],"high":[60],"level":[61],"(2%":[62],"total":[64],"protein)":[65],"during":[66],"exponential":[68],"phase":[69],"growth":[71],"upon":[72],"induction":[73],"by":[74,153,162,193],"sucrose.":[75],"Pulse-chase":[76],"experiments":[77],"showed":[78],"that":[79,133,209,221],"rate-limiting":[81],"step":[82,110,135,206],"(t1/2":[83,174],"=":[84,175],"120":[85],"s)":[86,177],"secretion":[89,112],"process":[90,142],"release":[93,205],"cell-associated":[96],"precursor":[97],"form":[98],"whose":[99],"has":[102],"been":[103],"cleaved.":[104],"efficiency":[106],"ultimate":[109],"decreased":[113],"dramatically":[114],"presence":[117,181],"metal":[120,185,234],"chelator":[121],"(EDTA)":[122],"or":[123],"when":[124],"cells":[126],"were":[127],"converted":[128],"to":[129,165],"protoplasts.":[130],"hypothesis":[132],"tightly":[137],"coupled":[138],"folding":[141,156,214,250],"occurring":[145],"within":[146,169],"cell":[148,198,226],"wall":[149,227],"environment":[150],"substantiated":[152],"vitro":[155],"studies.":[157],"unfolding-folding":[159],"transition,":[160],"monitored":[161],"resistance":[164],"proteolysis,":[166],"achieved":[168],"same":[171],"time":[172],"range":[173],"60":[176],"and":[178,237,239,249],"required":[179],"calcium.":[183],"This":[184,218],"requirement":[186],"could":[187],"possibly":[188],"be":[189],"satisfied":[190],"vivo":[192],"integrity":[195],"wall.":[199],"t1/2":[201],"double":[208],"levansucrase,":[211],"although":[212],"their":[213],"rates":[215],"are":[216],"similar.":[217],"perhaps":[219],"indicates":[220],"passage":[223],"through":[224],"may":[228],"depend":[229],"on":[230,240],"parietal":[231],"properties":[232,243],"(e.g.":[233],"ion":[235],"binding":[236],"porosity)":[238],"certain":[241],"intrinsic":[242],"protein":[246],"(molecular":[247],"mass":[248],"properties).":[251]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":2},{"year":2021,"cited_by_count":1},{"year":2018,"cited_by_count":1},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}