{"id":"https://openalex.org/W2137265502","doi":"https://doi.org/10.1128/jvi.23.1.53-60.1977","title":"Further Studies on Bacteriophage T4 DNA Synthesis in Sucrose-Plasmolyzed Cells","display_name":"Further Studies on Bacteriophage T4 DNA Synthesis in Sucrose-Plasmolyzed Cells","publication_year":1977,"publication_date":"1977-07-01","ids":{"openalex":"https://openalex.org/W2137265502","doi":"https://doi.org/10.1128/jvi.23.1.53-60.1977","mag":"2137265502","pmid":"https://pubmed.ncbi.nlm.nih.gov/328926","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/515799"},"language":"en","primary_location":{"id":"doi:10.1128/jvi.23.1.53-60.1977","doi":"https://doi.org/10.1128/jvi.23.1.53-60.1977","is_oa":false,"landing_page_url":"https://doi.org/10.1128/jvi.23.1.53-60.1977","pdf_url":null,"source":{"id":"https://openalex.org/S96615428","display_name":"Journal of Virology","issn_l":"0022-538X","issn":["0022-538X","1070-6321","1098-5514"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Virology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/515799","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5049558841","display_name":"Mary E. Stafford","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I138006243","display_name":"University of Arizona","ror":"https://ror.org/03m2x1q45","country_code":"US","type":"education","lineage":["https://openalex.org/I138006243"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Mary E. Stafford","raw_affiliation_strings":["Department of Biochemistry, College of Medicine, University of Arizona, Tucson, Arizona 85724"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, College of Medicine, University of Arizona, Tucson, Arizona 85724","institution_ids":["https://openalex.org/I138006243"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111273779","display_name":"G. Prem Veer Reddy","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I138006243","display_name":"University of Arizona","ror":"https://ror.org/03m2x1q45","country_code":"US","type":"education","lineage":["https://openalex.org/I138006243"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"G. Prem Veer Reddy","raw_affiliation_strings":["Department of Biochemistry, College of Medicine, University of Arizona, Tucson, Arizona 85724"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, College of Medicine, University of Arizona, Tucson, Arizona 85724","institution_ids":["https://openalex.org/I138006243"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5113535189","display_name":"Christopher K. Mathews","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I138006243","display_name":"University of Arizona","ror":"https://ror.org/03m2x1q45","country_code":"US","type":"education","lineage":["https://openalex.org/I138006243"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Christopher K. Mathews","raw_affiliation_strings":["Department of Biochemistry, College of Medicine, University of Arizona, Tucson, Arizona 85724"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, College of Medicine, University of Arizona, Tucson, Arizona 85724","institution_ids":["https://openalex.org/I138006243"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I138006243"],"apc_list":null,"apc_paid":null,"fwci":4.5992,"has_fulltext":false,"cited_by_count":15,"citation_normalized_percentile":{"value":0.95287774,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"23","issue":"1","first_page":"53","last_page":"60"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11048","display_name":"Bacteriophages and microbial interactions","score":0.9717000126838684,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11048","display_name":"Bacteriophages and microbial interactions","score":0.9717000126838684,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.016200000420212746,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10432","display_name":"DNA and Nucleic Acid Chemistry","score":0.0027000000700354576,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/bacteriophage-t4","display_name":"bacteriophage T4","score":0.996999979019165},{"id":"https://openalex.org/keywords/dna-synthesis","display_name":"DNA synthesis","score":0.8209999799728394},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.8309999704360962},{"id":"https://openalex.org/keywords/gene-1","display_name":"gene 1","score":0.7839999794960022},{"id":"https://openalex.org/keywords/nucleotide-incorporation","display_name":"nucleotide incorporation","score":0.7440000176429749},{"id":"https://openalex.org/keywords/deoxynucleotide-triphosphates","display_name":"deoxynucleotide triphosphates","score":0.8859999775886536}],"concepts":[{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.7691571116447449},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7591906785964966},{"id":"https://openalex.org/C64241487","wikidata":"https://www.wikidata.org/wiki/Q5205774","display_name":"DNA synthesis","level":3,"score":0.7010101079940796},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.6997561454772949},{"id":"https://openalex.org/C2776441376","wikidata":"https://www.wikidata.org/wiki/Q165028","display_name":"Bacteriophage","level":4,"score":0.616386890411377},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.5223958492279053},{"id":"https://openalex.org/C512185932","wikidata":"https://www.wikidata.org/wiki/Q28745","display_name":"Nucleotide","level":3,"score":0.5107345581054688},{"id":"https://openalex.org/C501734568","wikidata":"https://www.wikidata.org/wiki/Q42918","display_name":"Mutation","level":3,"score":0.4366985261440277},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.40715476870536804},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.40642523765563965},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.376482754945755},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.35283738374710083}],"mesh":[{"descriptor_ui":"D003090","descriptor_name":"Coliphages","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D003593","descriptor_name":"Cytoplasm","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003854","descriptor_name":"Deoxyribonucleotides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004261","descriptor_name":"DNA Replication","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004279","descriptor_name":"DNA, Viral","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005796","descriptor_name":"Genes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D009995","descriptor_name":"Osmosis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010641","descriptor_name":"Phenotype","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013395","descriptor_name":"Sucrose","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1128/jvi.23.1.53-60.1977","doi":"https://doi.org/10.1128/jvi.23.1.53-60.1977","is_oa":false,"landing_page_url":"https://doi.org/10.1128/jvi.23.1.53-60.1977","pdf_url":null,"source":{"id":"https://openalex.org/S96615428","display_name":"Journal of Virology","issn_l":"0022-538X","issn":["0022-538X","1070-6321","1098-5514"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Virology","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:515799","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/515799","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Virol","raw_type":"Text"},{"id":"pmid:328926","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/328926","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of virology","raw_type":"Journal Article"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:515799","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/515799","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Virol","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":22,"referenced_works":["https://openalex.org/W1480786735","https://openalex.org/W1487382673","https://openalex.org/W1495453928","https://openalex.org/W1500846337","https://openalex.org/W1502409094","https://openalex.org/W1530431083","https://openalex.org/W1547297235","https://openalex.org/W1585378712","https://openalex.org/W1802167367","https://openalex.org/W1834910841","https://openalex.org/W1895772557","https://openalex.org/W1946108117","https://openalex.org/W1965865857","https://openalex.org/W1966027411","https://openalex.org/W1976306991","https://openalex.org/W1977733455","https://openalex.org/W1989092114","https://openalex.org/W1992800978","https://openalex.org/W2067311394","https://openalex.org/W2069143571","https://openalex.org/W2072542500","https://openalex.org/W2131507346"],"related_works":["https://openalex.org/W1895772557","https://openalex.org/W1500846337","https://openalex.org/W1495453928","https://openalex.org/W2056139831","https://openalex.org/W1578058801","https://openalex.org/W1946108117","https://openalex.org/W1834910841","https://openalex.org/W1604031210","https://openalex.org/W2131507346","https://openalex.org/W2058000726"],"abstract_inverted_index":{"This":[0,211],"paper":[1],"describes":[2],"several":[3],"technical":[4],"improvements":[5],"in":[6,12,18,71,116,151],"the":[7,65,92,127,138,174,186,208,215],"sucrose-plasmolyzed":[8],"cell":[9,176,197],"system":[10,198],"used":[11],"earlier":[13],"experiments":[14],"on":[15,48],"DNA":[16,105,114,121,164,192,203,218],"synthesis":[17,115,165],"situ":[19],"with":[20,82,130,214],"Escherichia":[21],"coli":[22,148],"infected":[23,108],"by":[24],"DNA-defective":[25],"mutants":[26,68,97],"of":[27,50,67,95,104,140,160,230],"bacteriophage":[28],"T4":[29,96,156],"(W.":[30],"L.":[31],"Collinsworth":[32],"and":[33,75,77],"C.":[34],"K.":[35],"Mathews,":[36],"J.":[37],"Virol.":[38],"13:908-915,":[39],"1974).":[40],"Using":[41],"this":[42],"system,":[43,177],"which":[44],"is":[45,98,181,212],"based":[46],"primarily":[47],"that":[49,86,185,217],"M.":[51],"G.":[52],"Wovcha":[53],"et":[54,168],"al.":[55],"(Proc.":[56],"Natl.":[57],"Acad.":[58],"Sci.":[59],"U.S.A.":[60],"70:2196-2200,":[61],"1973),":[62],"we":[63,78,144],"reinvestigated":[64],"properties":[66],"bearing":[69],"lesions":[70,128],"genes":[72],"1,":[73],"41,":[74],"62,":[76],"resolved":[79],"some":[80],"disagreements":[81],"data":[83],"reported":[84],"from":[85,107],"laboratory.":[87],"We":[88],"also":[89,184],"asked":[90],"whether":[91],"DNA-delay":[93],"phenotype":[94],"related":[99],"to":[100,134,154,224,227],"possible":[101],"early":[102],"leakage":[103],"precursors":[106,122],"cells.":[109],"Such":[110],"cells":[111],"display":[112],"defective":[113,150],"situ,":[117],"even":[118],"when":[119],"ample":[120],"are":[123,221],"made":[124],"available.":[125],"Thus,":[126],"associated":[129],"these":[131],"mutations":[132],"seem":[133],"manifest":[135],"themselves":[136],"at":[137],"level":[139],"macromolecular":[141],"metabolism.":[142],"Similarly,":[143],"examined":[145],"an":[146],"E.":[147],"mutant":[149],"its":[152],"ability":[153],"support":[155],"production,":[157],"apparently":[158],"because":[159],"a":[161],"lesion":[162],"affecting":[163],"(L.":[166],"Simon":[167],"al.,":[169],"Nature":[170],"[London]":[171],"252:451-455).":[172],"In":[173],"plasmolyzed":[175,196],"reduced":[178],"nucleotide":[179],"incorporation":[180],"seen,":[182],"indicating":[183],"genetic":[187],"defect":[188],"does":[189],"not":[190],"involve":[191],"precursor":[193],"synthesis.":[194],"The":[195],"incorporates":[199],"deoxynucleotide":[200],"5'-monophosphates":[201],"into":[202],"severalfold":[204],"more":[205],"rapidly":[206],"than":[207],"corresponding":[209],"5'-triphosphates.":[210],"consistent":[213],"idea":[216],"precursor-synthesizing":[219],"enzymes":[220],"functionally":[222],"organized":[223],"shuttle":[225],"substrates":[226],"their":[228],"sites":[229],"utilization.":[231]},"counts_by_year":[{"year":2020,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}