{"id":"https://openalex.org/W2137102712","doi":"https://doi.org/10.1046/j.1365-2958.2000.01984.x","title":"Novel effects of a transposon insertion in the Vibrio fischeri glnD gene: defects in iron uptake and symbiotic persistence in addition to nitrogen utilization","display_name":"Novel effects of a transposon insertion in the Vibrio fischeri glnD gene: defects in iron uptake and symbiotic persistence in addition to nitrogen utilization","publication_year":2000,"publication_date":"2000-07-01","ids":{"openalex":"https://openalex.org/W2137102712","doi":"https://doi.org/10.1046/j.1365-2958.2000.01984.x","mag":"2137102712","pmid":"https://pubmed.ncbi.nlm.nih.gov/10931314"},"language":"en","primary_location":{"id":"doi:10.1046/j.1365-2958.2000.01984.x","doi":"https://doi.org/10.1046/j.1365-2958.2000.01984.x","is_oa":true,"landing_page_url":"https://doi.org/10.1046/j.1365-2958.2000.01984.x","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1046/j.1365-2958.2000.01984.x","source":{"id":"https://openalex.org/S102549058","display_name":"Molecular Microbiology","issn_l":"0950-382X","issn":["0950-382X","1365-2958"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular Microbiology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1046/j.1365-2958.2000.01984.x","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5025242251","display_name":"Joerg Graf","orcid":"https://orcid.org/0000-0001-5320-2712","observed_orcids":["https://orcid.org/0000-0001-5320-2712"]},"institutions":[{"id":"https://openalex.org/I1174212","display_name":"University of Southern California","ror":"https://ror.org/03taz7m60","country_code":"US","type":"education","lineage":["https://openalex.org/I1174212"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"J. Graf","raw_affiliation_strings":["Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089-0371, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089-0371, USA","institution_ids":["https://openalex.org/I1174212"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5062019784","display_name":"Edward G. Ruby","orcid":"https://orcid.org/0000-0002-4112-4830","observed_orcids":["https://orcid.org/0000-0002-4112-4830","https://orcid.org/0000-0002-5540-9028"]},"institutions":[{"id":"https://openalex.org/I1174212","display_name":"University of Southern California","ror":"https://ror.org/03taz7m60","country_code":"US","type":"education","lineage":["https://openalex.org/I1174212"]},{"id":"https://openalex.org/I117965899","display_name":"University of Hawai\u02bbi at M\u0101noa","ror":"https://ror.org/01wspgy28","country_code":"US","type":"education","lineage":["https://openalex.org/I117965899"]},{"id":"https://openalex.org/I1331384533","display_name":"University of Hawaii System","ror":"https://ror.org/03tzaeb71","country_code":"US","type":"education","lineage":["https://openalex.org/I1331384533"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"E. G. Ruby","raw_affiliation_strings":["Department of Biological Sciences , University of Southern California , Los Angeles , CA 90089-0371 , USA","Pacific Biomedical Research Center, Kewalo Marine Laboratory, University of Hawaii, 41 Ahui Street, Honolulu, Hawaii 96813, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Sciences , University of Southern California , Los Angeles , CA 90089-0371 , USA","institution_ids":["https://openalex.org/I1174212"]},{"raw_affiliation_string":"Pacific Biomedical Research Center, Kewalo Marine Laboratory, University of Hawaii, 41 Ahui Street, Honolulu, Hawaii 96813, USA","institution_ids":["https://openalex.org/I117965899","https://openalex.org/I1331384533"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5062019784"],"corresponding_institution_ids":["https://openalex.org/I1174212","https://openalex.org/I117965899","https://openalex.org/I1331384533"],"apc_list":{"value":4430,"currency":"USD","value_usd":4430},"apc_paid":null,"fwci":2.722,"has_fulltext":true,"cited_by_count":65,"citation_normalized_percentile":{"value":0.89870593,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"37","issue":"1","first_page":"168","last_page":"179"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.38089999556541443,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.38089999556541443,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12561","display_name":"bioluminescence and chemiluminescence research","score":0.23190000653266907,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11684","display_name":"Vibrio bacteria research studies","score":0.10930000245571136,"subfield":{"id":"https://openalex.org/subfields/1310","display_name":"Endocrinology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/vibrio-fischeri","display_name":"Vibrio fischeri","score":1.0},{"id":"https://openalex.org/keywords/nitrogen-utilization","display_name":"nitrogen utilization","score":0.5960000157356262},{"id":"https://openalex.org/keywords/siderophore-biosynthesis","display_name":"siderophore biosynthesis","score":0.8330000042915344},{"id":"https://openalex.org/keywords/iron-absorption","display_name":"iron absorption","score":0.7940000295639038},{"id":"https://openalex.org/keywords/iron-limitation","display_name":"iron limitation","score":0.9100000262260437},{"id":"https://openalex.org/keywords/euprymna-scolopes","display_name":"Euprymna scolopes","score":0.8970000147819519},{"id":"https://openalex.org/keywords/bacterial-colonization","display_name":"bacterial colonization","score":0.39800000190734863},{"id":"https://openalex.org/keywords/nitrogen-sensing","display_name":"nitrogen sensing","score":0.6029999852180481},{"id":"https://openalex.org/keywords/bioluminescence","display_name":"bioluminescence","score":0.628000020980835}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.9248298406600952},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.8255367279052734},{"id":"https://openalex.org/C2776154503","wikidata":"https://www.wikidata.org/wiki/Q753490","display_name":"Vibrio","level":3,"score":0.7397638559341431},{"id":"https://openalex.org/C108319773","wikidata":"https://www.wikidata.org/wiki/Q424747","display_name":"Siderophore","level":3,"score":0.7161990404129028},{"id":"https://openalex.org/C4918238","wikidata":"https://www.wikidata.org/wiki/Q121438","display_name":"Transposable element","level":4,"score":0.43545156717300415},{"id":"https://openalex.org/C127716648","wikidata":"https://www.wikidata.org/wiki/Q104053","display_name":"Phenotype","level":3,"score":0.43295755982398987},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.4273456037044525},{"id":"https://openalex.org/C5176359","wikidata":"https://www.wikidata.org/wiki/Q179924","display_name":"Bioluminescence","level":2,"score":0.4257308840751648},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.42532363533973694},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.4189285933971405},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.35453563928604126},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.21540987491607666}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002244","descriptor_name":"Carbon","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004251","descriptor_name":"DNA Transposable Elements","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D005816","descriptor_name":"Genetic Complementation Test","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007501","descriptor_name":"Iron","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D009584","descriptor_name":"Nitrogen","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D009713","descriptor_name":"Nucleotidyltransferases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D009713","descriptor_name":"Nucleotidyltransferases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010641","descriptor_name":"Phenotype","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013559","descriptor_name":"Symbiosis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D014733","descriptor_name":"Vibrio","qualifier_ui":"Q000145","qualifier_name":"classification","is_major_topic":false},{"descriptor_ui":"D014733","descriptor_name":"Vibrio","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D014733","descriptor_name":"Vibrio","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D014733","descriptor_name":"Vibrio","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":false},{"descriptor_ui":"D017262","descriptor_name":"Siderophores","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D049832","descriptor_name":"Decapodiformes","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":false},{"descriptor_ui":"D051922","descriptor_name":"PII Nitrogen Regulatory Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1046/j.1365-2958.2000.01984.x","doi":"https://doi.org/10.1046/j.1365-2958.2000.01984.x","is_oa":true,"landing_page_url":"https://doi.org/10.1046/j.1365-2958.2000.01984.x","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1046/j.1365-2958.2000.01984.x","source":{"id":"https://openalex.org/S102549058","display_name":"Molecular Microbiology","issn_l":"0950-382X","issn":["0950-382X","1365-2958"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular Microbiology","raw_type":"journal-article"},{"id":"pmid:10931314","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10931314","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular microbiology","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1046/j.1365-2958.2000.01984.x","doi":"https://doi.org/10.1046/j.1365-2958.2000.01984.x","is_oa":true,"landing_page_url":"https://doi.org/10.1046/j.1365-2958.2000.01984.x","pdf_url":"https://onlinelibrary.wiley.com/doi/pdfdirect/10.1046/j.1365-2958.2000.01984.x","source":{"id":"https://openalex.org/S102549058","display_name":"Molecular Microbiology","issn_l":"0950-382X","issn":["0950-382X","1365-2958"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Molecular Microbiology","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2365137226","display_name":"Vibrio Virulence Determinants in a Benign Colonization","funder_award_id":"R01RR012294","funder_id":"https://openalex.org/F4320337375","funder_display_name":"National Center for Research Resources"},{"id":"https://openalex.org/G342361386","display_name":null,"funder_award_id":"RR-12294","funder_id":"https://openalex.org/F4320337375","funder_display_name":"National Center for Research Resources"},{"id":"https://openalex.org/G4760511416","display_name":"Factors Mediating Bacteria-induced, Postembryonic Development","funder_award_id":"9904601","funder_id":"https://openalex.org/F4320306076","funder_display_name":"National Science Foundation"},{"id":"https://openalex.org/G8876996369","display_name":null,"funder_award_id":"N00014","funder_id":"https://openalex.org/F4320337345","funder_display_name":"Office of Naval Research"}],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"},{"id":"https://openalex.org/F4320308668","display_name":"University of Southern California","ror":"https://ror.org/03taz7m60"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337345","display_name":"Office of Naval Research","ror":"https://ror.org/00rk2pe57"},{"id":"https://openalex.org/F4320337375","display_name":"National Center for Research Resources","ror":"https://ror.org/04pw6fb54"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2137102712.pdf","grobid_xml":"https://content.openalex.org/works/W2137102712.grobid-xml"},"referenced_works_count":63,"referenced_works":["https://openalex.org/W182616941","https://openalex.org/W193267154","https://openalex.org/W276882634","https://openalex.org/W328595349","https://openalex.org/W332753678","https://openalex.org/W1414594946","https://openalex.org/W1488115944","https://openalex.org/W1493392084","https://openalex.org/W1500384048","https://openalex.org/W1505758906","https://openalex.org/W1512810258","https://openalex.org/W1532713345","https://openalex.org/W1567229177","https://openalex.org/W1594760003","https://openalex.org/W1661644842","https://openalex.org/W1879569910","https://openalex.org/W1910471001","https://openalex.org/W1941268961","https://openalex.org/W1943555456","https://openalex.org/W1967532932","https://openalex.org/W1976053068","https://openalex.org/W1992276931","https://openalex.org/W2004170283","https://openalex.org/W2018278033","https://openalex.org/W2023902482","https://openalex.org/W2026655267","https://openalex.org/W2048592989","https://openalex.org/W2054571400","https://openalex.org/W2058608544","https://openalex.org/W2073344480","https://openalex.org/W2080745257","https://openalex.org/W2081572587","https://openalex.org/W2085964029","https://openalex.org/W2100458748","https://openalex.org/W2107572522","https://openalex.org/W2108280568","https://openalex.org/W2110603871","https://openalex.org/W2113606247","https://openalex.org/W2117650481","https://openalex.org/W2119820314","https://openalex.org/W2121893220","https://openalex.org/W2126572569","https://openalex.org/W2134349239","https://openalex.org/W2136680449","https://openalex.org/W2139697588","https://openalex.org/W2142106844","https://openalex.org/W2143690144","https://openalex.org/W2148572341","https://openalex.org/W2150314694","https://openalex.org/W2152604863","https://openalex.org/W2161425804","https://openalex.org/W2164423936","https://openalex.org/W2166945327","https://openalex.org/W2169540382","https://openalex.org/W2177709753","https://openalex.org/W2205481277","https://openalex.org/W2220846580","https://openalex.org/W2233479487","https://openalex.org/W2301348645","https://openalex.org/W2314225431","https://openalex.org/W2337959802","https://openalex.org/W4255764366","https://openalex.org/W4402525860"],"related_works":["https://openalex.org/W7095364092","https://openalex.org/W2603577360","https://openalex.org/W2129970010","https://openalex.org/W1910471001","https://openalex.org/W2187388993","https://openalex.org/W2036610884","https://openalex.org/W2063101358","https://openalex.org/W4386032715","https://openalex.org/W7095306183","https://openalex.org/W2151936805"],"abstract_inverted_index":{"Vibrio":[0],"fischeri":[1,224],"is":[2,57],"the":[3,7,11,18,28,37,42,61,82,86,99,110,119,130,134,144,158,167,180,184,201,227],"sole":[4],"species":[5],"colonizing":[6],"light-emitting":[8],"organ":[9,21,132],"of":[10,22,39,44,53,63,76,88,160,192],"Hawaiian":[12],"squid,":[13,27],"Euprymna":[14],"scolopes.":[15],"Upon":[16],"entering":[17],"nascent":[19],"light":[20,131],"a":[23,51,58,73,209],"newly":[24],"hatched":[25],"juvenile":[26,115],"bacteria":[29],"undergo":[30],"morphological":[31],"and":[32,41,47,71,104,197,219],"physiological":[33],"changes":[34],"that":[35,56,166,225],"include":[36],"loss":[38],"flagellation":[40],"induction":[43],"bioluminescence.":[45],"These":[46,163],"other":[48],"events":[49],"reveal":[50],"pattern":[52],"genetic":[54,210],"regulation":[55],"response":[59],"to":[60,81,101,127,133,169,171,200,207],"colonization":[62,198],"host":[64],"tissue.":[65],"In":[66,79],"this":[67,91],"study,":[68],"we":[69],"isolated":[70],"characterized":[72],"glnD:mTn5Cm":[74,202],"mutant":[75,93,112,145],"V.":[77,223],"fischeri.":[78],"addition":[80],"predicted":[83],"defects":[84],"in":[85,98,129,179,187,222],"efficiency":[87,191],"nitrogen":[89,193,215],"utilization,":[90],"glnD":[92,111,185,228],"had":[94],"an":[95,176],"unexpected":[96],"reduction":[97],"ability":[100,168],"produce":[102],"siderophore":[103,217],"grow":[105],"under":[106,148],"iron-limiting":[107,149],"conditions.":[108],"Although":[109],"could":[113,154],"colonize":[114],"squid":[116],"normally":[117],"over":[118],"first":[120],"24":[121],"h,":[122],"it":[123],"was":[124,140,146],"subsequently":[125],"unable":[126],"persist":[128],"usual":[135],"extent.":[136],"This":[137],"persistence":[138],"phenotype":[139],"more":[141],"severe":[142],"if":[143],"pregrown":[147],"conditions":[150],"before":[151],"inoculation,":[152],"but":[153],"be":[155,175,208],"ameliorated":[156],"by":[157],"presence":[159],"excess":[161],"iron.":[162],"results":[164],"indicate":[165],"respond":[170],"iron":[172,195],"limitation":[173],"may":[174],"important":[177],"requirement":[178],"developing":[181],"symbiosis.":[182],"Supplying":[183],"gene":[186],"trans":[188],"restored":[189],"normal":[190],"use,":[194],"sequestration":[196],"phenotypes":[199],"mutant;":[203],"thus,":[204],"there":[205],"appears":[206],"and/or":[211],"metabolic":[212],"linkage":[213],"between":[214],"sensing,":[216],"synthesis":[218],"symbiosis":[220],"competence":[221],"involves":[226],"gene.":[229]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":2},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":4},{"year":2012,"cited_by_count":3}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}