{"id":"https://openalex.org/W2136648032","doi":"https://doi.org/10.1128/jb.170.1.365-371.1988","title":"Growth-rate-dependent expression and cloning of gnd alleles from natural isolates of Escherichia coli","display_name":"Growth-rate-dependent expression and cloning of gnd alleles from natural isolates of Escherichia coli","publication_year":1988,"publication_date":"1988-01-01","ids":{"openalex":"https://openalex.org/W2136648032","doi":"https://doi.org/10.1128/jb.170.1.365-371.1988","mag":"2136648032","pmid":"https://pubmed.ncbi.nlm.nih.gov/2826398","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/210651"},"language":"en","primary_location":{"id":"doi:10.1128/jb.170.1.365-371.1988","doi":"https://doi.org/10.1128/jb.170.1.365-371.1988","is_oa":false,"landing_page_url":"https://doi.org/10.1128/jb.170.1.365-371.1988","pdf_url":null,"source":{"id":"https://openalex.org/S133274750","display_name":"Journal of Bacteriology","issn_l":"0021-9193","issn":["0021-9193","1067-8832","1098-5530"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Bacteriology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/210651","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5071656846","display_name":"Gerard J. Barcak","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I79272384","display_name":"University of Maryland, Baltimore County","ror":"https://ror.org/02qskvh78","country_code":"US","type":"education","lineage":["https://openalex.org/I79272384"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"G J Barcak","raw_affiliation_strings":["Department of Biological Sciences, University of Maryland, Baltimore County 21228"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Sciences, University of Maryland, Baltimore County 21228","institution_ids":["https://openalex.org/I79272384"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111544659","display_name":"Richard E. Wolf","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I79272384","display_name":"University of Maryland, Baltimore County","ror":"https://ror.org/02qskvh78","country_code":"US","type":"education","lineage":["https://openalex.org/I79272384"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"R E Wolf","raw_affiliation_strings":["Department of Biological Sciences, University of Maryland, Baltimore County 21228"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biological Sciences, University of Maryland, Baltimore County 21228","institution_ids":["https://openalex.org/I79272384"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I79272384"],"apc_list":null,"apc_paid":null,"fwci":2.4404,"has_fulltext":false,"cited_by_count":24,"citation_normalized_percentile":{"value":0.89633462,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":null,"biblio":{"volume":"170","issue":"1","first_page":"365","last_page":"371"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9961000084877014,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9961000084877014,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10943","display_name":"Escherichia coli research studies","score":0.0007999999797903001,"subfield":{"id":"https://openalex.org/subfields/1310","display_name":"Endocrinology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/6-phosphogluconate-dehydrogenase","display_name":"6-phosphogluconate dehydrogenase","score":0.9829999804496765},{"id":"https://openalex.org/keywords/gnd-gene","display_name":"gnd gene","score":0.9779999852180481},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.9330000281333923},{"id":"https://openalex.org/keywords/salmonella-typhimurium","display_name":"Salmonella typhimurium","score":0.7889999747276306},{"id":"https://openalex.org/keywords/genetic-polymorphism","display_name":"genetic polymorphism","score":0.6539999842643738},{"id":"https://openalex.org/keywords/growth-rate-regulation","display_name":"growth rate control","score":0.5979999899864197},{"id":"https://openalex.org/keywords/isogenic-strains","display_name":"isogenic strains","score":0.6850000023841858},{"id":"https://openalex.org/keywords/restriction-mapping","display_name":"restriction mapping","score":0.5609999895095825},{"id":"https://openalex.org/keywords/restriction-site-polymorphism","display_name":"restriction site polymorphism","score":0.7770000100135803},{"id":"https://openalex.org/keywords/gene-divergence","display_name":"gene divergence","score":0.7400000095367432},{"id":"https://openalex.org/keywords/enzyme-activity","display_name":"enzyme activity","score":0.35199999809265137},{"id":"https://openalex.org/keywords/wild-strains","display_name":"wild isolate","score":0.4819999933242798}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.9411548972129822},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.8359798789024353},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.6849585771560669},{"id":"https://openalex.org/C128319531","wikidata":"https://www.wikidata.org/wiki/Q219715","display_name":"Restriction enzyme","level":3,"score":0.641005277633667},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.6388306617736816},{"id":"https://openalex.org/C180754005","wikidata":"https://www.wikidata.org/wiki/Q80726","display_name":"Allele","level":3,"score":0.6182349920272827},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.48385417461395264},{"id":"https://openalex.org/C121050878","wikidata":"https://www.wikidata.org/wiki/Q5135020","display_name":"Cloning (programming)","level":2,"score":0.44183364510536194},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.0},{"id":"https://openalex.org/C199360897","wikidata":"https://www.wikidata.org/wiki/Q9143","display_name":"Programming language","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000483","descriptor_name":"Alleles","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004262","descriptor_name":"DNA Restriction Enzymes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":false},{"descriptor_ui":"D005786","descriptor_name":"Gene Expression Regulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005798","descriptor_name":"Genes, Bacterial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010734","descriptor_name":"Phosphogluconate Dehydrogenase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D010734","descriptor_name":"Phosphogluconate Dehydrogenase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014161","descriptor_name":"Transduction, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1128/jb.170.1.365-371.1988","doi":"https://doi.org/10.1128/jb.170.1.365-371.1988","is_oa":false,"landing_page_url":"https://doi.org/10.1128/jb.170.1.365-371.1988","pdf_url":null,"source":{"id":"https://openalex.org/S133274750","display_name":"Journal of Bacteriology","issn_l":"0021-9193","issn":["0021-9193","1067-8832","1098-5530"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Bacteriology","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:210651","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/210651","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Bacteriol","raw_type":"Text"},{"id":"pmid:2826398","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2826398","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of bacteriology","raw_type":"Journal Article"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:210651","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/210651","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Bacteriol","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G187195762","display_name":null,"funder_award_id":"GM27113","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":35,"referenced_works":["https://openalex.org/W588812189","https://openalex.org/W1500869091","https://openalex.org/W1513792118","https://openalex.org/W1533456983","https://openalex.org/W1546783991","https://openalex.org/W1571619811","https://openalex.org/W1583488392","https://openalex.org/W1601687245","https://openalex.org/W1665551484","https://openalex.org/W1708604775","https://openalex.org/W1820644981","https://openalex.org/W1841558112","https://openalex.org/W1907149689","https://openalex.org/W1922660831","https://openalex.org/W1938165871","https://openalex.org/W1968496641","https://openalex.org/W1985754610","https://openalex.org/W2019410656","https://openalex.org/W2019691453","https://openalex.org/W2025822065","https://openalex.org/W2028622989","https://openalex.org/W2035702416","https://openalex.org/W2041661548","https://openalex.org/W2044952215","https://openalex.org/W2050499271","https://openalex.org/W2053337696","https://openalex.org/W2068321200","https://openalex.org/W2070385328","https://openalex.org/W2088330494","https://openalex.org/W2102105800","https://openalex.org/W2117277433","https://openalex.org/W2128635872","https://openalex.org/W2146532529","https://openalex.org/W2149527312","https://openalex.org/W2916217134"],"related_works":["https://openalex.org/W2070385328","https://openalex.org/W2019691453","https://openalex.org/W1500869091","https://openalex.org/W2097736786","https://openalex.org/W1665551484","https://openalex.org/W1533456983","https://openalex.org/W1520934266","https://openalex.org/W1922660831","https://openalex.org/W7101107343","https://openalex.org/W1583488392"],"abstract_inverted_index":{"6-Phosphogluconate":[0],"dehydrogenase":[1],"(6PGD),":[2],"encoded":[3],"by":[4,168,181,192],"gnd,":[5],"is":[6,101],"highly":[7],"polymorphic":[8],"among":[9],"isolates":[10],"of":[11,20,25,28,50,56,76,89,95,135,148,172],"Escherichia":[12],"coli":[13,36,43,111,159,175,201],"form":[14],"natural":[15],"populations.":[16],"As":[17],"a":[18],"means":[19],"characterizing":[21],"the":[22,26,34,51,54,80,87,129,136,145,149,164,173,190,196],"growth-rate-dependent":[23],"regulation":[24],"level":[27,55,75,100],"6PGD,":[29],"five":[30,109],"gnd":[31,104,123,160,197],"alleles,":[32],"including":[33],"E.":[35,42,110,158,174,200],"B/r":[37],"allele,":[38],"were":[39,138],"crossed":[40],"into":[41],"K-12":[44,202],"with":[45,119],"bacteriophage":[46],"P1.":[47],"In":[48],"each":[49],"isogenic":[52,90],"strains,":[53],"6PGD":[57],"was":[58,106,124,151],"two-":[59],"to":[60],"threefold":[61],"higher":[62],"in":[63,69,79,98],"cells":[64,70,82],"grown":[65,71],"on":[66,72,128],"glucose":[67],"than":[68,183],"acetate.":[73],"The":[74,92,103,132,157],"enzyme":[77,99],"activity":[78],"acetate-grown":[81],"varied":[83],"about":[84,169,193],"sixfold":[85],"within":[86],"set":[88],"strains.":[91],"physiological":[93],"importance":[94],"these":[96],"differences":[97],"discussed.":[102],"gene":[105,167,198],"cloned":[107],"from":[108,153,163,178,189,199],"strains":[112],"and":[113,117,126,144],"Salmonella":[114],"typhimurium":[115,166],"LT-2":[116],"mapped":[118],"twelve":[120],"restriction":[121,133,142,154],"endonucleases.":[122],"located":[125,207],"oriented":[127],"chromosomal":[130],"DNAs.":[131],"maps":[134],"genes":[137,150,161,176],"aligned":[139],"at":[140],"conserved":[141],"sites,":[143],"relative":[146],"divergence":[147],"estimated":[152],"site":[155],"polymorphisms.":[156],"differed":[162,177,188],"S.":[165],"11%.":[170],"Most":[171],"one":[179,186],"another":[180],"less":[182],"5%,":[184],"but":[185],"allele":[187],"others":[191],"10%.":[194],"Only":[195],"had":[203],"an":[204],"IS5":[205],"element":[206],"nearby.":[208]},"counts_by_year":[],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}