{"id":"https://openalex.org/W2136445320","doi":"https://doi.org/10.1016/j.jim.2004.04.008","title":"In-vitro protein evolution by ribosome display and mRNA display","display_name":"In-vitro protein evolution by ribosome display and mRNA display","publication_year":2004,"publication_date":"2004-06-02","ids":{"openalex":"https://openalex.org/W2136445320","doi":"https://doi.org/10.1016/j.jim.2004.04.008","mag":"2136445320","pmid":"https://pubmed.ncbi.nlm.nih.gov/15261571"},"language":"en","primary_location":{"id":"doi:10.1016/j.jim.2004.04.008","doi":"https://doi.org/10.1016/j.jim.2004.04.008","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.jim.2004.04.008","pdf_url":null,"source":{"id":"https://openalex.org/S201669150","display_name":"Journal of Immunological Methods","issn_l":"0022-1759","issn":["0022-1759","1872-7905"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Immunological Methods","raw_type":"journal-article"},"volume":null,"type":"review","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5002775376","display_name":"Da\u0161a Lipov\u0161ek","orcid":"https://orcid.org/0000-0001-9922-0019","observed_orcids":["https://orcid.org/0000-0001-9922-0019"]},"institutions":[{"id":"https://openalex.org/I63966007","display_name":"Massachusetts Institute of Technology","ror":"https://ror.org/042nb2s44","country_code":"US","type":"education","lineage":["https://openalex.org/I63966007"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Dasa Lipovsek","raw_affiliation_strings":["Biological Engineering Division, Massachusetts Institute of Technology, Cambridge 02139, USA. dlipovsek@ml1.net","Biological Engineering Division, Massachusetts Institute of Technology, Cambridge MA 02139, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Biological Engineering Division, Massachusetts Institute of Technology, Cambridge 02139, USA. dlipovsek@ml1.net","institution_ids":["https://openalex.org/I63966007"]},{"raw_affiliation_string":"Biological Engineering Division, Massachusetts Institute of Technology, Cambridge MA 02139, USA","institution_ids":["https://openalex.org/I63966007"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5083658123","display_name":"Andreas Pl\u00fcckthun","orcid":"https://orcid.org/0000-0003-4191-5306","observed_orcids":["https://orcid.org/0000-0003-4191-5306"]},"institutions":[{"id":"https://openalex.org/I202697423","display_name":"University of Zurich","ror":"https://ror.org/02crff812","country_code":"CH","type":"education","lineage":["https://openalex.org/I202697423"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Andreas Pl\u00fcckthun","raw_affiliation_strings":["Department of Biochemistry, University of Z\u00fcrich, Winterthurerstr. 190, CH-8057 Z\u00fcrich, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, University of Z\u00fcrich, Winterthurerstr. 190, CH-8057 Z\u00fcrich, Switzerland","institution_ids":["https://openalex.org/I202697423"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5002775376"],"corresponding_institution_ids":["https://openalex.org/I63966007"],"apc_list":{"value":2970,"currency":"USD","value_usd":2970},"apc_paid":null,"fwci":2.0977,"has_fulltext":false,"cited_by_count":364,"citation_normalized_percentile":{"value":0.89799197,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":98,"max":100},"biblio":{"volume":"290","issue":"1-2","first_page":"51","last_page":"67"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.3865000009536743,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.3865000009536743,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.3118000030517578,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":0.24410000443458557,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/in-vitro-protein-evolution","display_name":"in vitro protein evolution","score":0.8960000276565552},{"id":"https://openalex.org/keywords/ribosome-display","display_name":"ribosome display","score":0.9980000257492065},{"id":"https://openalex.org/keywords/mrna-display","display_name":"mRNA display","score":1.0},{"id":"https://openalex.org/keywords/ligand-selection","display_name":"ligand selection","score":0.6159999966621399},{"id":"https://openalex.org/keywords/affinity-maturation","display_name":"affinity maturation","score":0.5659999847412109},{"id":"https://openalex.org/keywords/puromycin","display_name":"puromycin","score":0.531000018119812},{"id":"https://openalex.org/keywords/peptide-display","display_name":"peptide display","score":0.42899999022483826},{"id":"https://openalex.org/keywords/antibody-display","display_name":"antibody display","score":0.7379999756813049},{"id":"https://openalex.org/keywords/fibronectin-type-iii-domain","display_name":"fibronectin type III domain","score":0.8330000042915344},{"id":"https://openalex.org/keywords/darwinian-evolution","display_name":"Darwinian evolution","score":0.4729999899864197}],"concepts":[{"id":"https://openalex.org/C88478588","wikidata":"https://www.wikidata.org/wiki/Q42244","display_name":"Ribosome","level":4,"score":0.6610580086708069},{"id":"https://openalex.org/C186268636","wikidata":"https://www.wikidata.org/wiki/Q896217","display_name":"Phage display","level":3,"score":0.6180204153060913},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.6037876605987549},{"id":"https://openalex.org/C2781377902","wikidata":"https://www.wikidata.org/wiki/Q21418769","display_name":"Affinity maturation","level":3,"score":0.5825188755989075},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.5200762152671814},{"id":"https://openalex.org/C2908542902","wikidata":"https://www.wikidata.org/wiki/Q440681","display_name":"Directed Molecular Evolution","level":5,"score":0.51534503698349},{"id":"https://openalex.org/C147816474","wikidata":"https://www.wikidata.org/wiki/Q169525","display_name":"Protein engineering","level":3,"score":0.5077189207077026},{"id":"https://openalex.org/C9418097","wikidata":"https://www.wikidata.org/wiki/Q440681","display_name":"Directed evolution","level":4,"score":0.49655681848526},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4959898889064789},{"id":"https://openalex.org/C149364088","wikidata":"https://www.wikidata.org/wiki/Q185917","display_name":"Translation (biology)","level":4,"score":0.47257721424102783},{"id":"https://openalex.org/C3675279","wikidata":"https://www.wikidata.org/wiki/Q211935","display_name":"Protein biosynthesis","level":2,"score":0.4587578773498535},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.43556058406829834},{"id":"https://openalex.org/C2779200571","wikidata":"https://www.wikidata.org/wiki/Q424696","display_name":"Puromycin","level":3,"score":0.42990684509277344},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3545498251914978},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3311936855316162},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.28956806659698486},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2578362226486206},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.18689343333244324},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.1716105043888092},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.16108182072639465},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.1562296748161316},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.15029305219650269}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012270","descriptor_name":"Ribosomes","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D012270","descriptor_name":"Ribosomes","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D012333","descriptor_name":"RNA, Messenger","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D015723","descriptor_name":"Gene Library","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019020","descriptor_name":"Directed Molecular Evolution","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D019151","descriptor_name":"Peptide Library","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/j.jim.2004.04.008","doi":"https://doi.org/10.1016/j.jim.2004.04.008","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.jim.2004.04.008","pdf_url":null,"source":{"id":"https://openalex.org/S201669150","display_name":"Journal of Immunological Methods","issn_l":"0022-1759","issn":["0022-1759","1872-7905"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Immunological Methods","raw_type":"journal-article"},{"id":"pmid:15261571","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/15261571","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of immunological methods","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":71,"referenced_works":["https://openalex.org/W11949986","https://openalex.org/W109945104","https://openalex.org/W1507805168","https://openalex.org/W1533325200","https://openalex.org/W1551404386","https://openalex.org/W1965671087","https://openalex.org/W1973974738","https://openalex.org/W1986038602","https://openalex.org/W1987768768","https://openalex.org/W1988037036","https://openalex.org/W1990423057","https://openalex.org/W1990557050","https://openalex.org/W1994318120","https://openalex.org/W1995946170","https://openalex.org/W1996427305","https://openalex.org/W1999960246","https://openalex.org/W2000455214","https://openalex.org/W2001302607","https://openalex.org/W2003071947","https://openalex.org/W2005551819","https://openalex.org/W2007109740","https://openalex.org/W2009179035","https://openalex.org/W2010235993","https://openalex.org/W2011513747","https://openalex.org/W2014812250","https://openalex.org/W2018627617","https://openalex.org/W2022228265","https://openalex.org/W2023167553","https://openalex.org/W2024298562","https://openalex.org/W2025551193","https://openalex.org/W2028370433","https://openalex.org/W2028493269","https://openalex.org/W2030189518","https://openalex.org/W2037651825","https://openalex.org/W2039486618","https://openalex.org/W2041375044","https://openalex.org/W2044682488","https://openalex.org/W2048558331","https://openalex.org/W2055760720","https://openalex.org/W2056374924","https://openalex.org/W2059998232","https://openalex.org/W2065344810","https://openalex.org/W2065625685","https://openalex.org/W2066574980","https://openalex.org/W2070136617","https://openalex.org/W2073517578","https://openalex.org/W2074805223","https://openalex.org/W2078923745","https://openalex.org/W2081132798","https://openalex.org/W2081835258","https://openalex.org/W2084938664","https://openalex.org/W2090062598","https://openalex.org/W2092803887","https://openalex.org/W2100419851","https://openalex.org/W2102212065","https://openalex.org/W2105888864","https://openalex.org/W2108049244","https://openalex.org/W2118743345","https://openalex.org/W2121543947","https://openalex.org/W2122689364","https://openalex.org/W2128692282","https://openalex.org/W2129812718","https://openalex.org/W2135827613","https://openalex.org/W2144886557","https://openalex.org/W2145354475","https://openalex.org/W2146939730","https://openalex.org/W2147862471","https://openalex.org/W2156037313","https://openalex.org/W2165447216","https://openalex.org/W2170551524","https://openalex.org/W4238858501"],"related_works":["https://openalex.org/W2018380171","https://openalex.org/W4252459729","https://openalex.org/W2024734204","https://openalex.org/W2023683208","https://openalex.org/W2998731295","https://openalex.org/W2354074252","https://openalex.org/W34502556","https://openalex.org/W3081287230","https://openalex.org/W2108364209","https://openalex.org/W2062384499"],"abstract_inverted_index":null,"counts_by_year":[{"year":2026,"cited_by_count":12},{"year":2025,"cited_by_count":9},{"year":2024,"cited_by_count":10},{"year":2023,"cited_by_count":11},{"year":2022,"cited_by_count":7},{"year":2021,"cited_by_count":8},{"year":2020,"cited_by_count":14},{"year":2019,"cited_by_count":10},{"year":2018,"cited_by_count":9},{"year":2017,"cited_by_count":14},{"year":2016,"cited_by_count":9},{"year":2015,"cited_by_count":12},{"year":2014,"cited_by_count":11},{"year":2013,"cited_by_count":18},{"year":2012,"cited_by_count":23}],"updated_date":"2026-11-13T13:42:25.865203","created_date":"2016-06-24T00:00:00"}