{"id":"https://openalex.org/W2136303506","doi":"https://doi.org/10.1016/s0021-9258(18)61424-3","title":"Crystallization and preliminary x-ray investigation of thymidine phosphorylase from Escherichia coli.","display_name":"Crystallization and preliminary x-ray investigation of thymidine phosphorylase from Escherichia coli.","publication_year":1987,"publication_date":"1987-03-01","ids":{"openalex":"https://openalex.org/W2136303506","doi":"https://doi.org/10.1016/s0021-9258(18)61424-3","mag":"2136303506","pmid":"https://pubmed.ncbi.nlm.nih.gov/3546318"},"language":"en","primary_location":{"id":"doi:10.1016/s0021-9258(18)61424-3","doi":"https://doi.org/10.1016/s0021-9258(18)61424-3","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(18)61424-3","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1016/s0021-9258(18)61424-3","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5113870921","display_name":"William J. Cook","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"W.J. Cook","raw_affiliation_strings":["Pharmacology, \u00a7Center for Macromolecular Crystallography, and llcomprehensive Cancer Center, University of A h m","Wellcome Research Laboratories, Research Triangle Park, North Carolina 27709","at Birmingham, University Station, Birmingham, Alabama 35294 and","From the Departments of $Pathology and"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pharmacology, \u00a7Center for Macromolecular Crystallography, and llcomprehensive Cancer Center, University of A h m","institution_ids":[]},{"raw_affiliation_string":"Wellcome Research Laboratories, Research Triangle Park, North Carolina 27709","institution_ids":[]},{"raw_affiliation_string":"at Birmingham, University Station, Birmingham, Alabama 35294 and","institution_ids":[]},{"raw_affiliation_string":"From the Departments of $Pathology and","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5073798859","display_name":"George W. Koszalka","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"G.W. Koszalka","raw_affiliation_strings":["Pharmacology, \u00a7Center for Macromolecular Crystallography, and llcomprehensive Cancer Center, University of A h m","Wellcome Research Laboratories, Research Triangle Park, North Carolina 27709","at Birmingham, University Station, Birmingham, Alabama 35294 and","From the Departments of $Pathology and"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pharmacology, \u00a7Center for Macromolecular Crystallography, and llcomprehensive Cancer Center, University of A h m","institution_ids":[]},{"raw_affiliation_string":"Wellcome Research Laboratories, Research Triangle Park, North Carolina 27709","institution_ids":[]},{"raw_affiliation_string":"at Birmingham, University Station, Birmingham, Alabama 35294 and","institution_ids":[]},{"raw_affiliation_string":"From the Departments of $Pathology and","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5054035902","display_name":"Willard W. Hall","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"W.W. Hall","raw_affiliation_strings":["Pharmacology, \u00a7Center for Macromolecular Crystallography, and llcomprehensive Cancer Center, University of A h m","Wellcome Research Laboratories, Research Triangle Park, North Carolina 27709","at Birmingham, University Station, Birmingham, Alabama 35294 and","From the Departments of $Pathology and"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pharmacology, \u00a7Center for Macromolecular Crystallography, and llcomprehensive Cancer Center, University of A h m","institution_ids":[]},{"raw_affiliation_string":"Wellcome Research Laboratories, Research Triangle Park, North Carolina 27709","institution_ids":[]},{"raw_affiliation_string":"at Birmingham, University Station, Birmingham, Alabama 35294 and","institution_ids":[]},{"raw_affiliation_string":"From the Departments of $Pathology and","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5112444756","display_name":"Charlene L. Burns","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"C.L. Burns","raw_affiliation_strings":["Pharmacology, \u00a7Center for Macromolecular Crystallography, and llcomprehensive Cancer Center, University of A h m","Wellcome Research Laboratories, Research Triangle Park, North Carolina 27709","at Birmingham, University Station, Birmingham, Alabama 35294 and","From the Departments of $Pathology and"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pharmacology, \u00a7Center for Macromolecular Crystallography, and llcomprehensive Cancer Center, University of A h m","institution_ids":[]},{"raw_affiliation_string":"Wellcome Research Laboratories, Research Triangle Park, North Carolina 27709","institution_ids":[]},{"raw_affiliation_string":"at Birmingham, University Station, Birmingham, Alabama 35294 and","institution_ids":[]},{"raw_affiliation_string":"From the Departments of $Pathology and","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5019164857","display_name":"S.E. Ealick","orcid":"https://orcid.org/0000-0001-8548-112X","observed_orcids":["https://orcid.org/0000-0001-8548-112X"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"S.E. Ealick","raw_affiliation_strings":["Pharmacology, \u00a7Center for Macromolecular Crystallography, and llcomprehensive Cancer Center, University of A h m","Wellcome Research Laboratories, Research Triangle Park, North Carolina 27709","at Birmingham, University Station, Birmingham, Alabama 35294 and","From the Departments of $Pathology and"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pharmacology, \u00a7Center for Macromolecular Crystallography, and llcomprehensive Cancer Center, University of A h m","institution_ids":[]},{"raw_affiliation_string":"Wellcome Research Laboratories, Research Triangle Park, North Carolina 27709","institution_ids":[]},{"raw_affiliation_string":"at Birmingham, University Station, Birmingham, Alabama 35294 and","institution_ids":[]},{"raw_affiliation_string":"From the Departments of $Pathology and","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":1.8649,"has_fulltext":false,"cited_by_count":12,"citation_normalized_percentile":{"value":0.85815154,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"262","issue":"8","first_page":"3788","last_page":"3789"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.9955999851226807,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.9955999851226807,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12827","display_name":"Biochemical and Molecular Research","score":0.003800000064074993,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10464","display_name":"Crystal structures of chemical compounds","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1604","display_name":"Inorganic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/thymidine-phosphorylase","display_name":"thymidine phosphorylase","score":1.0},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.9980000257492065},{"id":"https://openalex.org/keywords/protein-crystallization","display_name":"protein crystallization","score":0.7509999871253967},{"id":"https://openalex.org/keywords/crystal-structure","display_name":"crystal structure","score":0.7879999876022339},{"id":"https://openalex.org/keywords/tetragonal-crystal-form","display_name":"tetragonal crystal form","score":0.6269999742507935},{"id":"https://openalex.org/keywords/dimeric-protein","display_name":"dimeric protein","score":0.6349999904632568},{"id":"https://openalex.org/keywords/2-fold-symmetry","display_name":"2-fold symmetry","score":0.746999979019165},{"id":"https://openalex.org/keywords/ammonium-sulfate-solution","display_name":"ammonium sulfate solution","score":0.6890000104904175}],"concepts":[{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.8336973190307617},{"id":"https://openalex.org/C2776735402","wikidata":"https://www.wikidata.org/wiki/Q22038268","display_name":"Thymidine phosphorylase","level":3,"score":0.746302604675293},{"id":"https://openalex.org/C203036418","wikidata":"https://www.wikidata.org/wiki/Q284256","display_name":"Crystallization","level":2,"score":0.7397764921188354},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.499070405960083},{"id":"https://openalex.org/C2777108182","wikidata":"https://www.wikidata.org/wiki/Q422464","display_name":"Thymidine","level":3,"score":0.4562872052192688},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4530215263366699},{"id":"https://openalex.org/C204779464","wikidata":"https://www.wikidata.org/wiki/Q412061","display_name":"Glycogen phosphorylase","level":3,"score":0.4355669319629669},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.18008297681808472},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.17849969863891602},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.060291677713394165},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.056295424699783325}],"mesh":[{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D010430","descriptor_name":"Pentosyltransferases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013939","descriptor_name":"Thymidine Phosphorylase","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D014961","descriptor_name":"X-Ray Diffraction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1016/s0021-9258(18)61424-3","doi":"https://doi.org/10.1016/s0021-9258(18)61424-3","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(18)61424-3","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:3546318","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/3546318","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1016/s0021-9258(18)61424-3","doi":"https://doi.org/10.1016/s0021-9258(18)61424-3","is_oa":true,"landing_page_url":"https://doi.org/10.1016/s0021-9258(18)61424-3","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G142011812","display_name":null,"funder_award_id":"DE02670","funder_id":"https://openalex.org/F4320337365","funder_display_name":"National Institute of Dental and Craniofacial Research"},{"id":"https://openalex.org/G718442997","display_name":null,"funder_award_id":"CA-13 148","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"},{"id":"https://openalex.org/F4320337365","display_name":"National Institute of Dental and Craniofacial Research","ror":"https://ror.org/004a2wv92"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":12,"referenced_works":["https://openalex.org/W153702717","https://openalex.org/W1530672812","https://openalex.org/W1563631819","https://openalex.org/W1582709654","https://openalex.org/W1603423304","https://openalex.org/W1970775245","https://openalex.org/W1974384202","https://openalex.org/W1997774587","https://openalex.org/W2014694459","https://openalex.org/W2064276205","https://openalex.org/W2069048253","https://openalex.org/W2137302959"],"related_works":["https://openalex.org/W1582709654","https://openalex.org/W1517958332","https://openalex.org/W4238026009","https://openalex.org/W2073147919","https://openalex.org/W2057463030","https://openalex.org/W2066231300","https://openalex.org/W1987872475","https://openalex.org/W2203091258","https://openalex.org/W2054714271","https://openalex.org/W2010712548"],"abstract_inverted_index":{"Crystals":[0],"of":[1,12,59],"thymidine":[2],"phosphorylase":[3],"from":[4,10],"Escherichia":[5],"coli":[6],"have":[7],"been":[8],"grown":[9],"solutions":[11],"ammonium":[13],"sulfate.":[14],"The":[15,37,49],"crystals":[16,38],"are":[17,26,39],"tetragonal,":[18],"space":[19,61],"group":[20],"P4(1)2(1)2":[21],"or":[22],"P4(3)2(1)2;":[23],"the":[24,56,60],"axes":[25],"a":[27,52],"=":[28,33],"132.0":[29],"(1)":[30,35],"and":[31,44,54],"c":[32],"67.2":[34],"A.":[36],"quite":[40],"stable":[41],"to":[42],"x-rays":[43],"diffract":[45],"beyond":[46],"2.6-A":[47],"resolution.":[48],"molecule":[50],"is":[51],"dimer":[53],"utilizes":[55],"2-fold":[57],"symmetry":[58],"group,":[62],"resulting":[63],"in":[64],"one":[65],"subunit":[66],"per":[67],"asymmetric":[68],"unit.":[69]},"counts_by_year":[{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}