{"id":"https://openalex.org/W2136173365","doi":"https://doi.org/10.1128/jb.164.3.983-993.1985","title":"Cloning and DNA sequence of a plasmid-determined citrate utilization system in Escherichia coli","display_name":"Cloning and DNA sequence of a plasmid-determined citrate utilization system in Escherichia coli","publication_year":1985,"publication_date":"1985-12-01","ids":{"openalex":"https://openalex.org/W2136173365","doi":"https://doi.org/10.1128/jb.164.3.983-993.1985","mag":"2136173365","pmid":"https://pubmed.ncbi.nlm.nih.gov/2999088","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/219288"},"language":"en","primary_location":{"id":"doi:10.1128/jb.164.3.983-993.1985","doi":"https://doi.org/10.1128/jb.164.3.983-993.1985","is_oa":true,"landing_page_url":"https://doi.org/10.1128/jb.164.3.983-993.1985","pdf_url":"https://jb.asm.org/content/jb/164/3/983.full.pdf","source":{"id":"https://openalex.org/S133274750","display_name":"Journal of Bacteriology","issn_l":"0021-9193","issn":["0021-9193","1067-8832","1098-5530"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Bacteriology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://jb.asm.org/content/jb/164/3/983.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5066581922","display_name":"M Sasatsu","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"M Sasatsu","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5076063575","display_name":"Tapan K. Misra","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"T K Misra","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5011086961","display_name":"L Chu","orcid":"https://orcid.org/0009-0008-3079-4282","observed_orcids":["https://orcid.org/0009-0008-3079-4282"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"L Chu","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5058544654","display_name":"Richard A. Laddaga","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"R Laddaga","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5013549095","display_name":"Simon D. Silver","orcid":"https://orcid.org/0000-0002-5692-3125","observed_orcids":["https://orcid.org/0000-0002-5692-3125"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"S Silver","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":1.4584,"has_fulltext":false,"cited_by_count":53,"citation_normalized_percentile":{"value":0.82395537,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"164","issue":"3","first_page":"983","last_page":"993"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9945999979972839,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9945999979972839,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10943","display_name":"Escherichia coli research studies","score":0.0034000000450760126,"subfield":{"id":"https://openalex.org/subfields/2404","display_name":"Microbiology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10147","display_name":"Antibiotic Resistance in Bacteria","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/2726","display_name":"Microbiology (medical)"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/citrate-utilization","display_name":"citrate utilization","score":0.9900000095367432},{"id":"https://openalex.org/keywords/citrate-transport","display_name":"citrate transport","score":0.6470000147819519},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.8820000290870667},{"id":"https://openalex.org/keywords/open-reading-frames","display_name":"open reading frames","score":0.8159999847412109},{"id":"https://openalex.org/keywords/hydrophobic-polypeptides","display_name":"hydrophobic polypeptides","score":0.7770000100135803},{"id":"https://openalex.org/keywords/tetracycline-resistance-plasmid","display_name":"tetracycline resistance plasmid","score":0.8420000076293945},{"id":"https://openalex.org/keywords/plasmid-cloning","display_name":"plasmid cloning","score":0.5879999995231628},{"id":"https://openalex.org/keywords/dna-sequencing","display_name":"DNA sequencing","score":0.5090000033378601}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8372331857681274},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.6831152439117432},{"id":"https://openalex.org/C47289529","wikidata":"https://www.wikidata.org/wiki/Q908550","display_name":"Open reading frame","level":4,"score":0.651391863822937},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5947344899177551},{"id":"https://openalex.org/C130123407","wikidata":"https://www.wikidata.org/wiki/Q417754","display_name":"EcoRI","level":4,"score":0.5226776003837585},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.5080811977386475},{"id":"https://openalex.org/C2779758144","wikidata":"https://www.wikidata.org/wiki/Q4900470","display_name":"BglII","level":5,"score":0.4811490774154663},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.4575561285018921},{"id":"https://openalex.org/C170673058","wikidata":"https://www.wikidata.org/wiki/Q780686","display_name":"Restriction map","level":4,"score":0.45365726947784424},{"id":"https://openalex.org/C84148353","wikidata":"https://www.wikidata.org/wiki/Q863908","display_name":"Nucleic acid sequence","level":3,"score":0.4179626703262329},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3839568495750427},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.28230804204940796},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.09415847063064575}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001426","descriptor_name":"Bacterial Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001693","descriptor_name":"Biological Transport, Active","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002951","descriptor_name":"Citrates","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D004251","descriptor_name":"DNA Transposable Elements","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004262","descriptor_name":"DNA Restriction Enzymes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004269","descriptor_name":"DNA, Bacterial","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D015245","descriptor_name":"Deoxyribonuclease BamHI","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015252","descriptor_name":"Deoxyribonucleases, Type II Site-Specific","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D019343","descriptor_name":"Citric Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":5,"locations":[{"id":"doi:10.1128/jb.164.3.983-993.1985","doi":"https://doi.org/10.1128/jb.164.3.983-993.1985","is_oa":true,"landing_page_url":"https://doi.org/10.1128/jb.164.3.983-993.1985","pdf_url":"https://jb.asm.org/content/jb/164/3/983.full.pdf","source":{"id":"https://openalex.org/S133274750","display_name":"Journal of Bacteriology","issn_l":"0021-9193","issn":["0021-9193","1067-8832","1098-5530"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Bacteriology","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:1296314","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/219288","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:219288","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/219288","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Bacteriol","raw_type":"Text"},{"id":"pmid:2999088","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2999088","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of bacteriology","raw_type":"Journal Article"},{"id":"mag:2607109340","doi":null,"is_oa":false,"landing_page_url":"https://jb.asm.org/content/164/3/983.full.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"doi:10.1128/jb.164.3.983-993.1985","doi":"https://doi.org/10.1128/jb.164.3.983-993.1985","is_oa":true,"landing_page_url":"https://doi.org/10.1128/jb.164.3.983-993.1985","pdf_url":"https://jb.asm.org/content/jb/164/3/983.full.pdf","source":{"id":"https://openalex.org/S133274750","display_name":"Journal of Bacteriology","issn_l":"0021-9193","issn":["0021-9193","1067-8832","1098-5530"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Bacteriology","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G8342252475","display_name":"BIOMEDICAL RESEARCH SUPPORT","funder_award_id":"S07RR007054","funder_id":"https://openalex.org/F4320337375","funder_display_name":"National Center for Research Resources"}],"funders":[{"id":"https://openalex.org/F4320337375","display_name":"National Center for Research Resources","ror":"https://ror.org/04pw6fb54"}],"has_content":{"pdf":true,"grobid_xml":false},"content_urls":{"pdf":"https://content.openalex.org/works/W2136173365.pdf"},"referenced_works_count":37,"referenced_works":["https://openalex.org/W131551016","https://openalex.org/W1490909198","https://openalex.org/W1541805222","https://openalex.org/W1581132822","https://openalex.org/W1586243725","https://openalex.org/W1588342277","https://openalex.org/W1756500500","https://openalex.org/W1860166607","https://openalex.org/W1860624772","https://openalex.org/W1860681161","https://openalex.org/W1901316615","https://openalex.org/W1907450141","https://openalex.org/W1963872959","https://openalex.org/W1975304761","https://openalex.org/W1981014117","https://openalex.org/W1981371363","https://openalex.org/W1992042701","https://openalex.org/W2000595829","https://openalex.org/W2001769819","https://openalex.org/W2007006108","https://openalex.org/W2010017318","https://openalex.org/W2017205882","https://openalex.org/W2028701325","https://openalex.org/W2039622711","https://openalex.org/W2064686824","https://openalex.org/W2071424950","https://openalex.org/W2094563497","https://openalex.org/W2100137369","https://openalex.org/W2132278758","https://openalex.org/W2138270253","https://openalex.org/W2143246960","https://openalex.org/W2144233663","https://openalex.org/W2144634347","https://openalex.org/W2157300209","https://openalex.org/W2162217088","https://openalex.org/W2171506251","https://openalex.org/W2196869921"],"related_works":["https://openalex.org/W1588342277","https://openalex.org/W1901316615","https://openalex.org/W1992747285","https://openalex.org/W1558252570","https://openalex.org/W2265627682","https://openalex.org/W4245202375","https://openalex.org/W2105945609","https://openalex.org/W2327249938","https://openalex.org/W1560285367","https://openalex.org/W7097048330"],"abstract_inverted_index":{"The":[0,58,91],"citrate":[1,44,182,198,217],"utilization":[2,45,178],"determinant":[3],"from":[4,98,180,209],"a":[5,47,80,86,118,147,151,181,225],"large":[6],"200-kilobase":[7],"(kb)":[8],"naturally":[9],"occurring":[10],"plasmid":[11,20,28,37],"was":[12,61,70,79,201],"previously":[13],"cloned":[14,62],"into":[15,63],"the":[16,24,40,67,73,114,122,129,142,157,161,166,170,174,187,190,197,219],"PstI":[17,54],"site":[18],"of":[19,36,116,128,169,227],"vector":[21],"pBR325":[22],"creating":[23],"Cit+":[25,220],"tetracycline":[26],"resistance":[27],"pWR61":[29,38],"(15":[30],"kb).":[31],"Tn5":[32],"insertion":[33],"mutagenesis":[34],"analysis":[35],"limited":[39],"segment":[41],"responsible":[42],"for":[43,146,160,173],"to":[46,150,216,229],"4.8-kb":[48,59,130,191],"region":[49,159],"bordered":[50],"by":[51,72,186,203],"EcoRI":[52],"and":[53,66,139],"restriction":[55,136],"nuclease":[56],"sites.":[57],"fragment":[60,131,154,192],"phage":[64],"M13,":[65],"DNA":[68],"sequence":[69,78],"determined":[71],"dideoxyribonucleotide":[74],"method.":[75],"Within":[76],"this":[77,99],"1,296-base-pair":[81],"open":[82,100,110,124],"reading":[83,101,111,125,171],"frame":[84,102,112,172],"with":[85,113,135,194],"preceding":[87],"ribosomal":[88],"binding":[89],"site.":[90],"431-amino-acid":[92,162],"polypeptide":[93,120],"that":[94],"could":[95],"be":[96,104],"translated":[97],"would":[103],"highly":[105],"hydrophobic.":[106],"A":[107],"second":[108],"long":[109],"potential":[115],"encoding":[117],"379-amino-acid":[119,175],"preceded":[121],"larger":[123,195],"frame.":[126],"Portions":[127],"were":[132],"further":[133],"subcloned":[134],"endonucleases":[137],"BglII":[138],"BamHI,":[140],"reducing":[141],"minimum":[143],"size":[144],"needed":[145],"citrate-positive":[148],"phenotype":[149],"1.9-kb":[152],"BamHI-BglII":[153],"(which":[155],"includes":[156],"coding":[158],"polypeptide,":[163],"but":[164],"only":[165],"distal":[167],"2/3":[168],"polypeptide).":[176],"Citrate":[177],"results":[179],"transport":[183,199],"activity":[184,200],"encoded":[185],"plasmid.":[188],"With":[189],"(as":[193],"fragments)":[196],"inducible":[202],"growth":[204],"on":[205],"citrate.":[206],"On":[207],"transfer":[208],"glucose,":[210],"succinate,":[211],"malate,":[212],"or":[213],"glycerol":[214],"medium":[215],"medium,":[218],"Escherichia":[221],"coli":[222],"strains":[223],"showed":[224],"delay":[226],"36":[228],"48":[230],"h":[231],"before":[232],"growth.":[233]},"counts_by_year":[{"year":2014,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}
