{"id":"https://openalex.org/W2135066355","doi":"https://doi.org/10.1128/jb.177.12.3451-3454.1995","title":"DNA restriction-modification systems mediate plasmid maintenance","display_name":"DNA restriction-modification systems mediate plasmid maintenance","publication_year":1995,"publication_date":"1995-06-01","ids":{"openalex":"https://openalex.org/W2135066355","doi":"https://doi.org/10.1128/jb.177.12.3451-3454.1995","mag":"2135066355","pmid":"https://pubmed.ncbi.nlm.nih.gov/7768854","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/177048"},"language":"en","primary_location":{"id":"doi:10.1128/jb.177.12.3451-3454.1995","doi":"https://doi.org/10.1128/jb.177.12.3451-3454.1995","is_oa":false,"landing_page_url":"https://doi.org/10.1128/jb.177.12.3451-3454.1995","pdf_url":null,"source":{"id":"https://openalex.org/S133274750","display_name":"Journal of Bacteriology","issn_l":"0021-9193","issn":["0021-9193","1067-8832","1098-5530"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Bacteriology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://europepmc.org/pmc/articles/PMC177048","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5082811089","display_name":"Saulius Kulakauskas","orcid":"https://orcid.org/0000-0002-3466-8266","observed_orcids":["https://orcid.org/0000-0002-3466-8266"]},"institutions":[{"id":"https://openalex.org/I4210088668","display_name":"Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement","ror":"https://ror.org/003vg9w96","country_code":"FR","type":"government","lineage":["https://openalex.org/I4210088668"]},{"id":"https://openalex.org/I4387154544","display_name":"Institut National de la Recherche Agronomique","ror":"https://ror.org/01x3gbx83","country_code":"FR","type":"funder","lineage":["https://openalex.org/I4210088668","https://openalex.org/I4387154544"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"S Kulakauskas","raw_affiliation_strings":["Institut National de la Recherche Agronomique, Jouy-en-Josas, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institut National de la Recherche Agronomique, Jouy-en-Josas, France","institution_ids":["https://openalex.org/I4210088668","https://openalex.org/I4387154544"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5073734787","display_name":"Arvydas Lubys","orcid":"https://orcid.org/0000-0001-5050-503X","observed_orcids":["https://orcid.org/0000-0001-5050-503X"]},"institutions":[{"id":"https://openalex.org/I4210088668","display_name":"Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement","ror":"https://ror.org/003vg9w96","country_code":"FR","type":"government","lineage":["https://openalex.org/I4210088668"]},{"id":"https://openalex.org/I4387154544","display_name":"Institut National de la Recherche Agronomique","ror":"https://ror.org/01x3gbx83","country_code":"FR","type":"funder","lineage":["https://openalex.org/I4210088668","https://openalex.org/I4387154544"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"A Lubys","raw_affiliation_strings":["Institut National de la Recherche Agronomique, Jouy-en-Josas, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institut National de la Recherche Agronomique, Jouy-en-Josas, France","institution_ids":["https://openalex.org/I4210088668","https://openalex.org/I4387154544"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5108096651","display_name":"Stanislav Dusko Ehrlich","orcid":"https://orcid.org/0000-0002-7563-4046","observed_orcids":["https://orcid.org/0000-0002-7563-4046"]},"institutions":[{"id":"https://openalex.org/I4210088668","display_name":"Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement","ror":"https://ror.org/003vg9w96","country_code":"FR","type":"government","lineage":["https://openalex.org/I4210088668"]},{"id":"https://openalex.org/I4387154544","display_name":"Institut National de la Recherche Agronomique","ror":"https://ror.org/01x3gbx83","country_code":"FR","type":"funder","lineage":["https://openalex.org/I4210088668","https://openalex.org/I4387154544"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"S D Ehrlich","raw_affiliation_strings":["Institut National de la Recherche Agronomique, Jouy-en-Josas, France"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institut National de la Recherche Agronomique, Jouy-en-Josas, France","institution_ids":["https://openalex.org/I4210088668","https://openalex.org/I4387154544"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":3.295,"has_fulltext":false,"cited_by_count":100,"citation_normalized_percentile":{"value":0.9286441,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":87,"max":98},"biblio":{"volume":"177","issue":"12","first_page":"3451","last_page":"3454"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.9984999895095825,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11048","display_name":"Bacteriophages and microbial interactions","score":0.00039999998989515007,"subfield":{"id":"https://openalex.org/subfields/2406","display_name":"Virology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/plasmid-maintenance","display_name":"plasmid maintenance","score":0.9660000205039978},{"id":"https://openalex.org/keywords/ecori","display_name":"EcoRI","score":0.7739999890327454},{"id":"https://openalex.org/keywords/methyltransferase","display_name":"methyltransferase","score":0.781000018119812},{"id":"https://openalex.org/keywords/restriction-enzymes","display_name":"restriction enzymes","score":0.9810000061988831},{"id":"https://openalex.org/keywords/plasmid-loss","display_name":"plasmid loss","score":0.8870000243186951},{"id":"https://openalex.org/keywords/dna-double-strand-breaks","display_name":"DNA double-strand breaks","score":0.8379999995231628},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.8569999933242798},{"id":"https://openalex.org/keywords/bacillus-subtilis","display_name":"Bacillus subtilis","score":0.8899999856948853}],"concepts":[{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.9160599112510681},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8751398324966431},{"id":"https://openalex.org/C2777028655","wikidata":"https://www.wikidata.org/wiki/Q415601","display_name":"Endonuclease","level":3,"score":0.7709117531776428},{"id":"https://openalex.org/C130123407","wikidata":"https://www.wikidata.org/wiki/Q417754","display_name":"EcoRI","level":4,"score":0.7253772020339966},{"id":"https://openalex.org/C128319531","wikidata":"https://www.wikidata.org/wiki/Q219715","display_name":"Restriction enzyme","level":3,"score":0.6639289855957031},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.6393284797668457},{"id":"https://openalex.org/C2777272437","wikidata":"https://www.wikidata.org/wiki/Q131238","display_name":"Bacillus subtilis","level":3,"score":0.6206029653549194},{"id":"https://openalex.org/C109932267","wikidata":"https://www.wikidata.org/wiki/Q455079","display_name":"Plasmid preparation","level":5,"score":0.6035330295562744},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.5498313307762146},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.4737685024738312},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.36343130469322205},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.1563890278339386},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1267889440059662},{"id":"https://openalex.org/C205225487","wikidata":"https://www.wikidata.org/wiki/Q287604","display_name":"PBR322","level":4,"score":0.06687033176422119}],"mesh":[{"descriptor_ui":"D001412","descriptor_name":"Bacillus subtilis","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D001419","descriptor_name":"Bacteria","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D001419","descriptor_name":"Bacteria","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004262","descriptor_name":"DNA Restriction Enzymes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D015254","descriptor_name":"DNA Modification Methylases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D015280","descriptor_name":"DNA Restriction-Modification Enzymes","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true}],"locations_count":6,"locations":[{"id":"doi:10.1128/jb.177.12.3451-3454.1995","doi":"https://doi.org/10.1128/jb.177.12.3451-3454.1995","is_oa":false,"landing_page_url":"https://doi.org/10.1128/jb.177.12.3451-3454.1995","pdf_url":null,"source":{"id":"https://openalex.org/S133274750","display_name":"Journal of Bacteriology","issn_l":"0021-9193","issn":["0021-9193","1067-8832","1098-5530"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Bacteriology","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:150211","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC177048","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:177048","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/177048","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Bacteriol","raw_type":"Text"},{"id":"pmid:7768854","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7768854","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of bacteriology","raw_type":"Journal Article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.624.2382","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.624.2382","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://jb.asm.org/content/177/12/3451.full.pdf","raw_type":"text"},{"id":"pmh:oai:HAL:hal-02702831v1","doi":null,"is_oa":false,"landing_page_url":"https://hal.inrae.fr/hal-02702831","pdf_url":null,"source":{"id":"https://openalex.org/S4306402512","display_name":"HAL","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4405252976","host_organization_name":"Centre pour la Communication Scientifique Directe","host_organization_lineage":["https://openalex.org/I4405252976"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Journal of Bacteriology, 1995, 177 (12), pp.3451-3454","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":{"id":"pmh:oai:europepmc.org:150211","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC177048","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320338335","display_name":"H2020 European Research Council","ror":"https://ror.org/0472cxd90"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":38,"referenced_works":["https://openalex.org/W96976423","https://openalex.org/W319100838","https://openalex.org/W348060877","https://openalex.org/W609805730","https://openalex.org/W1546783991","https://openalex.org/W1773025083","https://openalex.org/W1876217832","https://openalex.org/W1890597695","https://openalex.org/W1966923113","https://openalex.org/W1977845046","https://openalex.org/W1982011873","https://openalex.org/W1983948427","https://openalex.org/W1985776863","https://openalex.org/W1988180185","https://openalex.org/W1988873625","https://openalex.org/W1991747510","https://openalex.org/W1995304122","https://openalex.org/W2010698950","https://openalex.org/W2022127268","https://openalex.org/W2024331073","https://openalex.org/W2024468809","https://openalex.org/W2035709881","https://openalex.org/W2043268620","https://openalex.org/W2045652176","https://openalex.org/W2062622230","https://openalex.org/W2066227684","https://openalex.org/W2069348889","https://openalex.org/W2077890483","https://openalex.org/W2079726617","https://openalex.org/W2123420786","https://openalex.org/W2128764801","https://openalex.org/W2144634347","https://openalex.org/W2159284935","https://openalex.org/W2165390912","https://openalex.org/W2395723962","https://openalex.org/W2916371305","https://openalex.org/W4211243493","https://openalex.org/W4244625433"],"related_works":["https://openalex.org/W4400457716","https://openalex.org/W2323299906","https://openalex.org/W2025104601","https://openalex.org/W2056173954","https://openalex.org/W2062965982","https://openalex.org/W1975294323","https://openalex.org/W2152447567","https://openalex.org/W1598076755","https://openalex.org/W2085877407","https://openalex.org/W2170718546"],"abstract_inverted_index":{"Two":[0],"plasmid-carried":[1],"restriction-modification":[2],"(R-M)":[3],"systems,":[4],"EcoRI":[5],"(from":[6,13],"pMB1":[7],"of":[8,15,32,38,47,63,86,94,113,127],"Escherichia":[9],"coli)":[10],"and":[11,27,71,92],"Bsp6I":[12],"pXH13":[14],"Bacillus":[16,28],"sp.":[17],"strain":[18],"RFL6),":[19],"enhance":[20],"plasmid":[21,57,119],"segregational":[22,58],"stability":[23,59,120],"in":[24,41,97],"E.":[25],"coli":[26],"subtilis,":[29],"respectively.":[30],"Inactivation":[31],"the":[33,36,39,44,48,77,87,98,107,111,114,128],"endonuclease":[34],"or":[35],"presence":[37],"methylase":[40,88],"trans":[42],"abolish":[43],"stabilizing":[45],"activity":[46],"R-M":[49,54,129],"systems.":[50,130],"We":[51],"propose":[52],"that":[53],"systems":[55],"mediate":[56],"by":[60,76,106],"postsegregational":[61],"killing":[62],"plasmid-free":[64,115],"cells.":[65,116],"Plasmid-encoded":[66],"methyltransferase":[67],"modifies":[68],"host":[69],"DNA":[70],"thus":[72],"prevents":[73],"its":[74],"digestion":[75],"restriction":[78],"endonuclease.":[79],"Plasmid":[80],"loss":[81],"entails":[82],"degradation":[83],"and/or":[84],"dilution":[85],"during":[89],"cell":[90],"growth":[91],"appearance":[93],"unmethylated":[95],"sites":[96,105],"chromosome.":[99],"Double-strand":[100],"breaks,":[101],"introduced":[102],"at":[103],"these":[104],"endonuclease,":[108],"eventually":[109],"cause":[110],"death":[112],"Contribution":[117],"to":[118],"is":[121],"a":[122],"previously":[123],"unrecognized":[124],"biological":[125],"role":[126]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":2},{"year":2022,"cited_by_count":6},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":7},{"year":2019,"cited_by_count":3},{"year":2017,"cited_by_count":4},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":3},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":3}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}