{"id":"https://openalex.org/W2134076458","doi":"https://doi.org/10.1002/9780470123188.ch5","title":"Mechanistic Issues in Asparagine Synthetase Catalysis","display_name":"Mechanistic Issues in Asparagine Synthetase Catalysis","publication_year":1998,"publication_date":"1998-01-01","ids":{"openalex":"https://openalex.org/W2134076458","doi":"https://doi.org/10.1002/9780470123188.ch5","mag":"2134076458","pmid":"https://pubmed.ncbi.nlm.nih.gov/9559053"},"language":"en","primary_location":{"id":"doi:10.1002/9780470123188.ch5","doi":"https://doi.org/10.1002/9780470123188.ch5","is_oa":false,"landing_page_url":"https://doi.org/10.1002/9780470123188.ch5","pdf_url":null,"source":{"id":"https://openalex.org/S2280564","display_name":"Advances in enzymology and related areas of molecular biology/Advances in enzymology and related subjects","issn_l":"0065-258X","issn":["0065-258X","1934-4694"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","norway-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Advances in Enzymology - and Related Areas of Molecular Biology","raw_type":"other"},"volume":{"id":"https://openalex.org/W4230073920","display_name":"Advances in Enzymology and Related Areas of Molecular Biology"},"type":"other","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5033047262","display_name":"Nigel G. J. Richards","orcid":"https://orcid.org/0000-0002-0375-0881","observed_orcids":["https://orcid.org/0000-0002-0375-0881"]},"institutions":[{"id":"https://openalex.org/I33213144","display_name":"University of Florida","ror":"https://ror.org/02y3ad647","country_code":"US","type":"education","lineage":["https://openalex.org/I33213144"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Nigel G. J. Richards","raw_affiliation_strings":["Department of Chemistry, University of Florida, Gainesville 32611, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of Florida, Gainesville 32611, USA","institution_ids":["https://openalex.org/I33213144"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111549684","display_name":"Sheldon M. Schuster","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I33213144","display_name":"University of Florida","ror":"https://ror.org/02y3ad647","country_code":"US","type":"education","lineage":["https://openalex.org/I33213144"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Sheldon M. Schuster","raw_affiliation_strings":["Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL 32610, and Interdisciplinary Center for Biotechnology Research, University of Florida, Gainesville, FL 32611"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL 32610, and Interdisciplinary Center for Biotechnology Research, University of Florida, Gainesville, FL 32611","institution_ids":["https://openalex.org/I33213144"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I33213144"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":103,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":"72","issue":null,"first_page":"145","last_page":"198"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12770","display_name":"Amino Acid Enzymes and Metabolism","score":0.9761000275611877,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12770","display_name":"Amino Acid Enzymes and Metabolism","score":0.9761000275611877,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12827","display_name":"Biochemical and Molecular Research","score":0.014999999664723873,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12191","display_name":"Chemical Reaction Mechanisms","score":0.004800000227987766,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/asparagine-synthetase","display_name":"asparagine synthetase","score":0.9990000128746033},{"id":"https://openalex.org/keywords/enzyme-mechanism","display_name":"enzyme mechanism","score":0.9010000228881836},{"id":"https://openalex.org/keywords/amidotransferase","display_name":"amidotransferase","score":0.8510000109672546},{"id":"https://openalex.org/keywords/glutamine-utilization","display_name":"glutamine utilization","score":0.7149999737739563},{"id":"https://openalex.org/keywords/nitrogen-transfer","display_name":"nitrogen transfer","score":0.675000011920929},{"id":"https://openalex.org/keywords/amide-synthesis","display_name":"amide synthesis","score":0.9490000009536743},{"id":"https://openalex.org/keywords/active-site-structure","display_name":"active site structure","score":0.7799999713897705},{"id":"https://openalex.org/keywords/asparagine-biosynthesis","display_name":"asparagine biosynthesis","score":0.671999990940094}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7390345335006714},{"id":"https://openalex.org/C2775895851","wikidata":"https://www.wikidata.org/wiki/Q185906","display_name":"Asparagine","level":3,"score":0.6784973740577698},{"id":"https://openalex.org/C2780240386","wikidata":"https://www.wikidata.org/wiki/Q21131459","display_name":"Asparagine synthetase","level":4,"score":0.5997490882873535},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5606036186218262},{"id":"https://openalex.org/C161790260","wikidata":"https://www.wikidata.org/wiki/Q82264","display_name":"Catalysis","level":2,"score":0.4387401044368744},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.2924366593360901}],"mesh":[{"descriptor_ui":"D000255","descriptor_name":"Adenosine Triphosphate","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000641","descriptor_name":"Ammonia","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D001217","descriptor_name":"Aspartate-Ammonia Ligase","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":false},{"descriptor_ui":"D001217","descriptor_name":"Aspartate-Ammonia Ligase","qualifier_ui":"Q000145","qualifier_name":"classification","is_major_topic":false},{"descriptor_ui":"D001217","descriptor_name":"Aspartate-Ammonia Ligase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D001224","descriptor_name":"Aspartic Acid","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005057","descriptor_name":"Eukaryotic Cells","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D005973","descriptor_name":"Glutamine","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011387","descriptor_name":"Prokaryotic Cells","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D017386","descriptor_name":"Sequence Homology, Amino Acid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1002/9780470123188.ch5","doi":"https://doi.org/10.1002/9780470123188.ch5","is_oa":false,"landing_page_url":"https://doi.org/10.1002/9780470123188.ch5","pdf_url":null,"source":{"id":"https://openalex.org/S2280564","display_name":"Advances in enzymology and related areas of molecular biology/Advances in enzymology and related subjects","issn_l":"0065-258X","issn":["0065-258X","1934-4694"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","norway-1"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"book series"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Advances in Enzymology - and Related Areas of Molecular Biology","raw_type":"other"},{"id":"pmid:9559053","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/9559053","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Advances in enzymology and related areas of molecular biology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G634854420","display_name":null,"funder_award_id":"CA 28725","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"},{"id":"https://openalex.org/G6542064354","display_name":"ASPARAGINE BIOSYNTHESIS IN NORMAL AND TUMOR CELLS","funder_award_id":"7r01ca028725-17","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":167,"referenced_works":["https://openalex.org/W87543932","https://openalex.org/W91939836","https://openalex.org/W124504601","https://openalex.org/W204889168","https://openalex.org/W953150473","https://openalex.org/W991054534","https://openalex.org/W1250969645","https://openalex.org/W1480430356","https://openalex.org/W1481034093","https://openalex.org/W1485335314","https://openalex.org/W1485390666","https://openalex.org/W1488019265","https://openalex.org/W1501315042","https://openalex.org/W1501964283","https://openalex.org/W1513574545","https://openalex.org/W1516942594","https://openalex.org/W1517798915","https://openalex.org/W1519974564","https://openalex.org/W1526681768","https://openalex.org/W1527324425","https://openalex.org/W1535761892","https://openalex.org/W1545434090","https://openalex.org/W1547455977","https://openalex.org/W1552086546","https://openalex.org/W1555552538","https://openalex.org/W1558633123","https://openalex.org/W1567415408","https://openalex.org/W1568123347","https://openalex.org/W1584692552","https://openalex.org/W1590325990","https://openalex.org/W1598071384","https://openalex.org/W1604647115","https://openalex.org/W1620404326","https://openalex.org/W1652691445","https://openalex.org/W1772749380","https://openalex.org/W1837322864","https://openalex.org/W1845792010","https://openalex.org/W1848163973","https://openalex.org/W1850662735","https://openalex.org/W1863336654","https://openalex.org/W1871750915","https://openalex.org/W1942359435","https://openalex.org/W1945724510","https://openalex.org/W1964155223","https://openalex.org/W1966347760","https://openalex.org/W1967352806","https://openalex.org/W1967676770","https://openalex.org/W1968854847","https://openalex.org/W1969153299","https://openalex.org/W1970260534","https://openalex.org/W1975460230","https://openalex.org/W1976062391","https://openalex.org/W1976881268","https://openalex.org/W1976952882","https://openalex.org/W1979013471","https://openalex.org/W1980199589","https://openalex.org/W1981723997","https://openalex.org/W1982079867","https://openalex.org/W1982935511","https://openalex.org/W1983751826","https://openalex.org/W1986579209","https://openalex.org/W1987170844","https://openalex.org/W1989158891","https://openalex.org/W1989637555","https://openalex.org/W1990539942","https://openalex.org/W1995137974","https://openalex.org/W1995494888","https://openalex.org/W1999119754","https://openalex.org/W2000388754","https://openalex.org/W2003886194","https://openalex.org/W2006386700","https://openalex.org/W2008909147","https://openalex.org/W2009310436","https://openalex.org/W2013851046","https://openalex.org/W2016462809","https://openalex.org/W2017770922","https://openalex.org/W2018055593","https://openalex.org/W2021894945","https://openalex.org/W2022556344","https://openalex.org/W2023722477","https://openalex.org/W2025621500","https://openalex.org/W2028066599","https://openalex.org/W2029657967","https://openalex.org/W2034358495","https://openalex.org/W2035705250","https://openalex.org/W2039516505","https://openalex.org/W2041603274","https://openalex.org/W2041683509","https://openalex.org/W2044430464","https://openalex.org/W2045752794","https://openalex.org/W2047353155","https://openalex.org/W2048854640","https://openalex.org/W2049364854","https://openalex.org/W2051440400","https://openalex.org/W2052448379","https://openalex.org/W2052560048","https://openalex.org/W2053623997","https://openalex.org/W2054114498","https://openalex.org/W2054329140","https://openalex.org/W2055171649","https://openalex.org/W2055563809","https://openalex.org/W2056157112","https://openalex.org/W2057093907","https://openalex.org/W2057508801","https://openalex.org/W2057589234","https://openalex.org/W2057831386","https://openalex.org/W2058565981","https://openalex.org/W2062762024","https://openalex.org/W2062938604","https://openalex.org/W2065267648","https://openalex.org/W2065862200","https://openalex.org/W2067887310","https://openalex.org/W2068018119","https://openalex.org/W2068372163","https://openalex.org/W2068493918","https://openalex.org/W2069526310","https://openalex.org/W2073867256","https://openalex.org/W2073959398","https://openalex.org/W2075316114","https://openalex.org/W2075323803","https://openalex.org/W2077379637","https://openalex.org/W2077821797","https://openalex.org/W2077973224","https://openalex.org/W2079257271","https://openalex.org/W2079633544","https://openalex.org/W2080525555","https://openalex.org/W2080977102","https://openalex.org/W2086243648","https://openalex.org/W2088749908","https://openalex.org/W2089153412","https://openalex.org/W2090736922","https://openalex.org/W2091313109","https://openalex.org/W2092642545","https://openalex.org/W2093679528","https://openalex.org/W2094424998","https://openalex.org/W2094647907","https://openalex.org/W2094755816","https://openalex.org/W2101971141","https://openalex.org/W2108475341","https://openalex.org/W2108733359","https://openalex.org/W2110274263","https://openalex.org/W2112636022","https://openalex.org/W2119280527","https://openalex.org/W2120931628","https://openalex.org/W2131124255","https://openalex.org/W2136371489","https://openalex.org/W2138619808","https://openalex.org/W2150377117","https://openalex.org/W2152708819","https://openalex.org/W2152873608","https://openalex.org/W2160756311","https://openalex.org/W2160922424","https://openalex.org/W2162537143","https://openalex.org/W2214925661","https://openalex.org/W2227208022","https://openalex.org/W2253221655","https://openalex.org/W2318340157","https://openalex.org/W2318433867","https://openalex.org/W2344317983","https://openalex.org/W2408255017","https://openalex.org/W2437285653","https://openalex.org/W2489736657","https://openalex.org/W2951685061","https://openalex.org/W4211007828","https://openalex.org/W4211023727","https://openalex.org/W4239833500","https://openalex.org/W4251672032"],"related_works":["https://openalex.org/W1997966635","https://openalex.org/W1995129726","https://openalex.org/W2056157112","https://openalex.org/W2025202389","https://openalex.org/W2052560048","https://openalex.org/W2021262190","https://openalex.org/W2051440400","https://openalex.org/W1964155223","https://openalex.org/W2076423011","https://openalex.org/W2040553779"],"abstract_inverted_index":{"The":[0],"enzymatic":[1],"synthesis":[2],"of":[3,24,67,81],"asparagine":[4,82],"is":[5,30,93,108],"an":[6],"ATP-dependent":[7],"process":[8],"that":[9],"utilizes":[10],"the":[11,61,64,68,79,88,97,102],"nitrogen":[12,107],"atom":[13],"derived":[14],"from":[15],"either":[16],"glutamine":[17,73],"or":[18],"ammonia.":[19],"Despite":[20],"a":[21],"long":[22],"history":[23],"kinetic":[25],"and":[26,75,100],"mechanistic":[27],"investigation,":[28],"there":[29],"no":[31],"universally":[32],"accepted":[33],"catalytic":[34],"mechanism":[35,104],"for":[36],"this":[37],"seemingly":[38],"straightforward":[39],"carboxyl":[40],"group":[41],"activating":[42],"enzyme,":[43],"especially":[44],"as":[45],"regards":[46],"those":[47],"steps":[48],"immediately":[49],"preceding":[50],"amide":[51],"bond":[52],"formation.":[53],"This":[54],"chapter":[55],"considers":[56],"four":[57],"issues":[58],"dealing":[59],"with":[60],"mechanism:":[62],"(a)":[63],"structural":[65],"organization":[66],"active":[69],"site(s)":[70],"partaking":[71],"in":[72,90],"utilization":[74],"aspartate":[76],"activation;":[77],"(b)":[78],"relationship":[80],"synthetase":[83],"to":[84,95],"other":[85],"amidotransferases;":[86],"(c)":[87],"way":[89],"which":[91,106],"ATP":[92],"used":[94],"activate":[96],"beta-carboxyl":[98],"group;":[99],"(d)":[101],"detailed":[103],"by":[105],"transferred.":[109]},"counts_by_year":[{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":5},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":6},{"year":2021,"cited_by_count":5},{"year":2020,"cited_by_count":5},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":4},{"year":2017,"cited_by_count":4},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":2},{"year":2014,"cited_by_count":4},{"year":2013,"cited_by_count":6},{"year":2012,"cited_by_count":3}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}
