{"id":"https://openalex.org/W2133608465","doi":"https://doi.org/10.1016/j.jchromb.2009.02.038","title":"Countercurrent chromatographic separation and purification of various ribonucleases using a small-scale cross-axis coil planet centrifuge with aqueous\u2013aqueous polymer phase systems","display_name":"Countercurrent chromatographic separation and purification of various ribonucleases using a small-scale cross-axis coil planet centrifuge with aqueous\u2013aqueous polymer phase systems","publication_year":2009,"publication_date":"2009-02-25","ids":{"openalex":"https://openalex.org/W2133608465","doi":"https://doi.org/10.1016/j.jchromb.2009.02.038","mag":"2133608465","pmid":"https://pubmed.ncbi.nlm.nih.gov/19272844","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2745059"},"language":"en","primary_location":{"id":"doi:10.1016/j.jchromb.2009.02.038","doi":"https://doi.org/10.1016/j.jchromb.2009.02.038","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.jchromb.2009.02.038","pdf_url":null,"source":{"id":"https://openalex.org/S103248676","display_name":"Journal of Chromatography B","issn_l":"1570-0232","issn":["1570-0232","1873-376X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Chromatography B","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2745059","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5063156265","display_name":"Kazufusa Shinomiya","orcid":"https://orcid.org/0000-0001-7897-6546","observed_orcids":["https://orcid.org/0000-0001-7897-6546"]},"institutions":[{"id":"https://openalex.org/I104946051","display_name":"Nihon University","ror":"https://ror.org/05jk51a88","country_code":"JP","type":"education","lineage":["https://openalex.org/I104946051"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Kazufusa Shinomiya","raw_affiliation_strings":["College of Pharmacy, Nihon University, 7-7-1, Narashinodai, Funabashi-shi, Chiba 274-8555, Japan. shinomiya.kazufusa@nihon-u.ac.jp","College of Pharmacy, Nihon University, 7-7-1 Narashinodai, Funabashi-shi, Chiba, 274-8555, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Pharmacy, Nihon University, 7-7-1, Narashinodai, Funabashi-shi, Chiba 274-8555, Japan. shinomiya.kazufusa@nihon-u.ac.jp","institution_ids":["https://openalex.org/I104946051"]},{"raw_affiliation_string":"College of Pharmacy, Nihon University, 7-7-1 Narashinodai, Funabashi-shi, Chiba, 274-8555, Japan","institution_ids":["https://openalex.org/I104946051"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5001665110","display_name":"Hiroko Kobayashi","orcid":"https://orcid.org/0000-0003-3348-733X","observed_orcids":["https://orcid.org/0000-0003-3348-733X"]},"institutions":[{"id":"https://openalex.org/I104946051","display_name":"Nihon University","ror":"https://ror.org/05jk51a88","country_code":"JP","type":"education","lineage":["https://openalex.org/I104946051"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Hiroko Kobayashi","raw_affiliation_strings":["College of Pharmacy, Nihon University, 7-7-1, Narashinodai, Funabashi-shi, Chiba 274-8555, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Pharmacy, Nihon University, 7-7-1, Narashinodai, Funabashi-shi, Chiba 274-8555, Japan","institution_ids":["https://openalex.org/I104946051"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5072860574","display_name":"Naomi Motoyoshi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I104946051","display_name":"Nihon University","ror":"https://ror.org/05jk51a88","country_code":"JP","type":"education","lineage":["https://openalex.org/I104946051"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Naomi Motoyoshi","raw_affiliation_strings":["College of Pharmacy, Nihon University, 7-7-1, Narashinodai, Funabashi-shi, Chiba 274-8555, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Pharmacy, Nihon University, 7-7-1, Narashinodai, Funabashi-shi, Chiba 274-8555, Japan","institution_ids":["https://openalex.org/I104946051"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5090651380","display_name":"Norio Inokuchi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I104946051","display_name":"Nihon University","ror":"https://ror.org/05jk51a88","country_code":"JP","type":"education","lineage":["https://openalex.org/I104946051"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Norio Inokuchi","raw_affiliation_strings":["College of Pharmacy, Nihon University, 7-7-1, Narashinodai, Funabashi-shi, Chiba 274-8555, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Pharmacy, Nihon University, 7-7-1, Narashinodai, Funabashi-shi, Chiba 274-8555, Japan","institution_ids":["https://openalex.org/I104946051"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108467779","display_name":"Kazuya Nakagomi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I87531838","display_name":"Teikyo University","ror":"https://ror.org/01gaw2478","country_code":"JP","type":"education","lineage":["https://openalex.org/I87531838"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Kazuya Nakagomi","raw_affiliation_strings":["Faculty of Pharmaceutical Sciences, Teikyo University, 1091-1, Suwarashi, Sagamiko, Sagamihara-shi, Kanagawa 199-0195, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Faculty of Pharmaceutical Sciences, Teikyo University, 1091-1, Suwarashi, Sagamiko, Sagamihara-shi, Kanagawa 199-0195, Japan","institution_ids":["https://openalex.org/I87531838"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5029128268","display_name":"Yoichiro Ito","orcid":"https://orcid.org/0000-0001-9018-295X","observed_orcids":["https://orcid.org/0000-0001-9018-295X"]},"institutions":[{"id":"https://openalex.org/I1299303238","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88","country_code":"US","type":"government","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238"]},{"id":"https://openalex.org/I4210106489","display_name":"National Heart, Lung, and Blood Institute","ror":"https://ror.org/012pb6c26","country_code":"US","type":"facility","lineage":["https://openalex.org/I1299022934","https://openalex.org/I1299303238","https://openalex.org/I4210106489"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yoichiro Ito","raw_affiliation_strings":["Bioseparation Technology Laboratory, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, 10 Center Drive, Building 10, Room 8N230, Bethesda, MD 20892-1762, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Bioseparation Technology Laboratory, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, 10 Center Drive, Building 10, Room 8N230, Bethesda, MD 20892-1762, USA","institution_ids":["https://openalex.org/I1299303238","https://openalex.org/I4210106489"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":4,"corresponding_author_ids":["https://openalex.org/A5063156265"],"corresponding_institution_ids":["https://openalex.org/I104946051"],"apc_list":{"value":3640,"currency":"USD","value_usd":3640},"apc_paid":null,"fwci":1.2941,"has_fulltext":false,"cited_by_count":9,"citation_normalized_percentile":{"value":0.79814355,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"877","issue":"10","first_page":"955","last_page":"960"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T13128","display_name":"Chromatography in Natural Products","score":0.9545999765396118,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T13128","display_name":"Chromatography in Natural Products","score":0.9545999765396118,"subfield":{"id":"https://openalex.org/subfields/1602","display_name":"Analytical Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10908","display_name":"Analytical Chemistry and Chromatography","score":0.020600000396370888,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11936","display_name":"Chemical and Physical Properties in Aqueous Solutions","score":0.010099999606609344,"subfield":{"id":"https://openalex.org/subfields/1506","display_name":"Filtration and Separation"},"field":{"id":"https://openalex.org/fields/15","display_name":"Chemical Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/ribonuclease","display_name":"ribonuclease","score":0.8399999737739563},{"id":"https://openalex.org/keywords/countercurrent-chromatography","display_name":"countercurrent chromatography","score":0.9369999766349792},{"id":"https://openalex.org/keywords/polyethylene-glycol","display_name":"polyethylene glycol","score":0.8920000195503235},{"id":"https://openalex.org/keywords/rnase-a","display_name":"ribonuclease A","score":0.8029999732971191},{"id":"https://openalex.org/keywords/rnase-b","display_name":"RNase B","score":0.9990000128746033},{"id":"https://openalex.org/keywords/enzyme-purification","display_name":"enzyme purification","score":0.8009999990463257},{"id":"https://openalex.org/keywords/preparative-scale-separation","display_name":"preparative-scale separation","score":0.7429999709129333},{"id":"https://openalex.org/keywords/enzyme-activity-retention","display_name":"enzyme activity retention","score":0.6240000128746033}],"concepts":[{"id":"https://openalex.org/C2781289165","wikidata":"https://www.wikidata.org/wiki/Q3381491","display_name":"Potassium phosphate","level":2,"score":0.8568044900894165},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.7745181322097778},{"id":"https://openalex.org/C184651966","wikidata":"https://www.wikidata.org/wiki/Q906356","display_name":"Aqueous solution","level":2,"score":0.7225541472434998},{"id":"https://openalex.org/C134337899","wikidata":"https://www.wikidata.org/wiki/Q11158","display_name":"Monobasic acid","level":2,"score":0.6270765066146851},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6038569808006287},{"id":"https://openalex.org/C2776964284","wikidata":"https://www.wikidata.org/wiki/Q410083","display_name":"Polyethylene glycol","level":2,"score":0.5401315689086914},{"id":"https://openalex.org/C122191521","wikidata":"https://www.wikidata.org/wiki/Q11158","display_name":"Dibasic acid","level":2,"score":0.48400041460990906},{"id":"https://openalex.org/C44280652","wikidata":"https://www.wikidata.org/wiki/Q104837","display_name":"Phase (matter)","level":2,"score":0.4819304943084717},{"id":"https://openalex.org/C521977710","wikidata":"https://www.wikidata.org/wiki/Q81163","display_name":"Polymer","level":2,"score":0.4530309736728668},{"id":"https://openalex.org/C54400483","wikidata":"https://www.wikidata.org/wiki/Q1793202","display_name":"PEG ratio","level":2,"score":0.4458846151828766},{"id":"https://openalex.org/C2780657338","wikidata":"https://www.wikidata.org/wiki/Q191134","display_name":"Centrifuge","level":2,"score":0.42505744099617004},{"id":"https://openalex.org/C149556010","wikidata":"https://www.wikidata.org/wiki/Q4782870","display_name":"Aqueous two-phase system","level":3,"score":0.4243772625923157},{"id":"https://openalex.org/C188027245","wikidata":"https://www.wikidata.org/wiki/Q750446","display_name":"Polymer chemistry","level":1,"score":0.15136104822158813},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.11672469973564148},{"id":"https://openalex.org/C185544564","wikidata":"https://www.wikidata.org/wiki/Q81197","display_name":"Nuclear physics","level":1,"score":0.0},{"id":"https://openalex.org/C162324750","wikidata":"https://www.wikidata.org/wiki/Q8134","display_name":"Economics","level":0,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C10138342","wikidata":"https://www.wikidata.org/wiki/Q43015","display_name":"Finance","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D002498","descriptor_name":"Centrifugation","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D003377","descriptor_name":"Countercurrent Distribution","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010710","descriptor_name":"Phosphates","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011092","descriptor_name":"Polyethylene Glycols","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D012260","descriptor_name":"Ribonucleases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D012260","descriptor_name":"Ribonucleases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D012260","descriptor_name":"Ribonucleases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012260","descriptor_name":"Ribonucleases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D017680","descriptor_name":"Potassium Compounds","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D020076","descriptor_name":"Pleurotus","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":false},{"descriptor_ui":"D020539","descriptor_name":"Sequence Analysis, Protein","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1016/j.jchromb.2009.02.038","doi":"https://doi.org/10.1016/j.jchromb.2009.02.038","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.jchromb.2009.02.038","pdf_url":null,"source":{"id":"https://openalex.org/S103248676","display_name":"Journal of Chromatography B","issn_l":"1570-0232","issn":["1570-0232","1873-376X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Chromatography B","raw_type":"journal-article"},{"id":"pmid:19272844","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/19272844","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of chromatography. B, Analytical technologies in the biomedical and life sciences","raw_type":"Evaluation Study"},{"id":"pmh:oai:pubmedcentral.nih.gov:2745059","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2745059","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"J Chromatogr B Analyt Technol Biomed Life Sci","raw_type":"Text"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:2745059","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2745059","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"J Chromatogr B Analyt Technol Biomed Life Sci","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G4705346009","display_name":null,"funder_award_id":"Z99 HL999999","funder_id":"https://openalex.org/F4320332161","funder_display_name":"National Institutes of Health"}],"funders":[{"id":"https://openalex.org/F4320320912","display_name":"Ministry of Education, Culture, Sports, Science and Technology","ror":"https://ror.org/048rj2z13"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":20,"referenced_works":["https://openalex.org/W70746119","https://openalex.org/W123779693","https://openalex.org/W193825228","https://openalex.org/W230386500","https://openalex.org/W1484821298","https://openalex.org/W1686232265","https://openalex.org/W1972176589","https://openalex.org/W1987454130","https://openalex.org/W2004108676","https://openalex.org/W2018289835","https://openalex.org/W2043962451","https://openalex.org/W2061087032","https://openalex.org/W2063968553","https://openalex.org/W2074339402","https://openalex.org/W2087046941","https://openalex.org/W2091049953","https://openalex.org/W2100837269","https://openalex.org/W2397982552","https://openalex.org/W2895340958","https://openalex.org/W4232534952"],"related_works":["https://openalex.org/W2067512308","https://openalex.org/W2034105240","https://openalex.org/W1775009445","https://openalex.org/W2000019500","https://openalex.org/W2025008226","https://openalex.org/W2015438241","https://openalex.org/W1996398267","https://openalex.org/W123779693","https://openalex.org/W2053352111","https://openalex.org/W2324345795"],"abstract_inverted_index":null,"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}