{"id":"https://openalex.org/W2132875813","doi":"https://doi.org/10.1016/j.jbiotec.2010.02.015","title":"Maximizing the ex vivo expansion of human mesenchymal stem cells using a microcarrier-based stirred culture system","display_name":"Maximizing the ex vivo expansion of human mesenchymal stem cells using a microcarrier-based stirred culture system","publication_year":2010,"publication_date":"2010-02-26","ids":{"openalex":"https://openalex.org/W2132875813","doi":"https://doi.org/10.1016/j.jbiotec.2010.02.015","mag":"2132875813","pmid":"https://pubmed.ncbi.nlm.nih.gov/20188771"},"language":"en","primary_location":{"id":"doi:10.1016/j.jbiotec.2010.02.015","doi":"https://doi.org/10.1016/j.jbiotec.2010.02.015","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.jbiotec.2010.02.015","pdf_url":null,"source":{"id":"https://openalex.org/S182099265","display_name":"Journal of Biotechnology","issn_l":"0168-1656","issn":["0168-1656","1873-4863"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biotechnology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://doi.org/10.1016/j.jbiotec.2010.02.015.","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5085232897","display_name":"Gemma Eibes","orcid":"https://orcid.org/0000-0003-2432-6323","observed_orcids":["https://orcid.org/0000-0003-2432-6323"]},"institutions":[{"id":"https://openalex.org/I4210167387","display_name":"Institute for Biotechnology and Bioengineering","ror":"https://ror.org/05ws8g470","country_code":"PT","type":"facility","lineage":["https://openalex.org/I4210167387"]},{"id":"https://openalex.org/I4387152517","display_name":"Instituto Superior T\u00e9cnico","ror":"https://ror.org/03db2by73","country_code":"PT","type":"education","lineage":["https://openalex.org/I141596103","https://openalex.org/I4387152517"]}],"countries":["PT"],"is_corresponding":false,"raw_author_name":"Gemma Eibes","raw_affiliation_strings":["IBB - Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior T\u00e9cnico, Av. Rovisco Pais, 1049-001 Lisboa, Portugal"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"IBB - Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior T\u00e9cnico, Av. Rovisco Pais, 1049-001 Lisboa, Portugal","institution_ids":["https://openalex.org/I4210167387","https://openalex.org/I4387152517"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101829624","display_name":"Francisco dos Santos","orcid":"https://orcid.org/0000-0002-2046-2742","observed_orcids":["https://orcid.org/0000-0002-2046-2742"]},"institutions":[{"id":"https://openalex.org/I4210167387","display_name":"Institute for Biotechnology and Bioengineering","ror":"https://ror.org/05ws8g470","country_code":"PT","type":"facility","lineage":["https://openalex.org/I4210167387"]},{"id":"https://openalex.org/I4387152517","display_name":"Instituto Superior T\u00e9cnico","ror":"https://ror.org/03db2by73","country_code":"PT","type":"education","lineage":["https://openalex.org/I141596103","https://openalex.org/I4387152517"]}],"countries":["PT"],"is_corresponding":false,"raw_author_name":"Francisco dos Santos","raw_affiliation_strings":["IBB \u2013 Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior T\u00e9cnico, Av. Rovisco Pais, 1049-001 Lisboa, Portugal"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"IBB \u2013 Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior T\u00e9cnico, Av. Rovisco Pais, 1049-001 Lisboa, Portugal","institution_ids":["https://openalex.org/I4210167387","https://openalex.org/I4387152517"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100890528","display_name":"Pedro Z. Andrade","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210167387","display_name":"Institute for Biotechnology and Bioengineering","ror":"https://ror.org/05ws8g470","country_code":"PT","type":"facility","lineage":["https://openalex.org/I4210167387"]},{"id":"https://openalex.org/I4387152517","display_name":"Instituto Superior T\u00e9cnico","ror":"https://ror.org/03db2by73","country_code":"PT","type":"education","lineage":["https://openalex.org/I141596103","https://openalex.org/I4387152517"]}],"countries":["PT"],"is_corresponding":false,"raw_author_name":"Pedro Z. Andrade","raw_affiliation_strings":["IBB \u2013 Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior T\u00e9cnico, Av. Rovisco Pais, 1049-001 Lisboa, Portugal"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"IBB \u2013 Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior T\u00e9cnico, Av. Rovisco Pais, 1049-001 Lisboa, Portugal","institution_ids":["https://openalex.org/I4210167387","https://openalex.org/I4387152517"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5056108053","display_name":"Joana S. Boura","orcid":"https://orcid.org/0000-0002-6026-197X","observed_orcids":["https://orcid.org/0000-0002-6026-197X"]},"institutions":[{"id":"https://openalex.org/I4210167387","display_name":"Institute for Biotechnology and Bioengineering","ror":"https://ror.org/05ws8g470","country_code":"PT","type":"facility","lineage":["https://openalex.org/I4210167387"]},{"id":"https://openalex.org/I4387152517","display_name":"Instituto Superior T\u00e9cnico","ror":"https://ror.org/03db2by73","country_code":"PT","type":"education","lineage":["https://openalex.org/I141596103","https://openalex.org/I4387152517"]}],"countries":["PT"],"is_corresponding":false,"raw_author_name":"Joana S. Boura","raw_affiliation_strings":["IBB \u2013 Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior T\u00e9cnico, Av. Rovisco Pais, 1049-001 Lisboa, Portugal"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"IBB \u2013 Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior T\u00e9cnico, Av. Rovisco Pais, 1049-001 Lisboa, Portugal","institution_ids":["https://openalex.org/I4210167387","https://openalex.org/I4387152517"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108519372","display_name":"Manuel M. Abecasis","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210103180","display_name":"Instituto Portugu\u00eas de Oncologia Francisco Gentil","ror":"https://ror.org/00r7b5b77","country_code":"PT","type":"healthcare","lineage":["https://openalex.org/I4210103180"]}],"countries":["PT"],"is_corresponding":false,"raw_author_name":"Manuel M.A. Abecasis","raw_affiliation_strings":["Instituto Portugu\u00eas de Oncologia de Lisboa Francisco Gentil, E.P.E., R. Prof. Lima Basto, 1099-023 Lisboa, Portugal"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Instituto Portugu\u00eas de Oncologia de Lisboa Francisco Gentil, E.P.E., R. Prof. Lima Basto, 1099-023 Lisboa, Portugal","institution_ids":["https://openalex.org/I4210103180"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5060339975","display_name":"Claudia Lobato da Silva","orcid":"https://orcid.org/0000-0002-1091-7651","observed_orcids":["https://orcid.org/0000-0002-1091-7651"]},"institutions":[{"id":"https://openalex.org/I4210167387","display_name":"Institute for Biotechnology and Bioengineering","ror":"https://ror.org/05ws8g470","country_code":"PT","type":"facility","lineage":["https://openalex.org/I4210167387"]},{"id":"https://openalex.org/I4387152517","display_name":"Instituto Superior T\u00e9cnico","ror":"https://ror.org/03db2by73","country_code":"PT","type":"education","lineage":["https://openalex.org/I141596103","https://openalex.org/I4387152517"]}],"countries":["PT"],"is_corresponding":false,"raw_author_name":"Cl\u00e1udia Lobato da Silva","raw_affiliation_strings":["IBB \u2013 Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior T\u00e9cnico, Av. Rovisco Pais, 1049-001 Lisboa, Portugal"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"IBB \u2013 Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior T\u00e9cnico, Av. Rovisco Pais, 1049-001 Lisboa, Portugal","institution_ids":["https://openalex.org/I4210167387","https://openalex.org/I4387152517"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5007145023","display_name":"Joaquim M. S. Cabral","orcid":"https://orcid.org/0000-0002-2405-5845","observed_orcids":["https://orcid.org/0000-0002-2405-5845"]},"institutions":[{"id":"https://openalex.org/I4210167387","display_name":"Institute for Biotechnology and Bioengineering","ror":"https://ror.org/05ws8g470","country_code":"PT","type":"facility","lineage":["https://openalex.org/I4210167387"]},{"id":"https://openalex.org/I4387152517","display_name":"Instituto Superior T\u00e9cnico","ror":"https://ror.org/03db2by73","country_code":"PT","type":"education","lineage":["https://openalex.org/I141596103","https://openalex.org/I4387152517"]}],"countries":["PT"],"is_corresponding":true,"raw_author_name":"Joaquim M.S. Cabral","raw_affiliation_strings":["IBB \u2013 Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior T\u00e9cnico, Av. Rovisco Pais, 1049-001 Lisboa, Portugal"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"IBB \u2013 Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior T\u00e9cnico, Av. Rovisco Pais, 1049-001 Lisboa, Portugal","institution_ids":["https://openalex.org/I4210167387","https://openalex.org/I4387152517"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5007145023"],"corresponding_institution_ids":["https://openalex.org/I4210167387","https://openalex.org/I4387152517"],"apc_list":{"value":4360,"currency":"USD","value_usd":4360},"apc_paid":null,"fwci":4.8539,"has_fulltext":false,"cited_by_count":184,"citation_normalized_percentile":{"value":0.96327961,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":91,"max":100},"biblio":{"volume":"146","issue":"4","first_page":"194","last_page":"197"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10176","display_name":"Mesenchymal stem cell research","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10176","display_name":"Mesenchymal stem cell research","score":0.9980000257492065,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10864","display_name":"Fluid Dynamics and Mixing","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/1507","display_name":"Fluid Flow and Transfer Processes"},"field":{"id":"https://openalex.org/fields/15","display_name":"Chemical Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10345","display_name":"Hematopoietic Stem Cell Transplantation","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/2720","display_name":"Hematology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/mesenchymal-stem-cells","display_name":"mesenchymal stem cells","score":0.9900000095367432},{"id":"https://openalex.org/keywords/ex-vivo-expansion","display_name":"ex vivo expansion","score":0.6949999928474426},{"id":"https://openalex.org/keywords/microcarrier-culture","display_name":"microcarrier culture","score":0.7760000228881836},{"id":"https://openalex.org/keywords/gelatin-microcarriers","display_name":"gelatin microcarriers","score":0.8529999852180481},{"id":"https://openalex.org/keywords/spinner-flasks","display_name":"spinner flasks","score":0.7099999785423279},{"id":"https://openalex.org/keywords/bone-marrow","display_name":"bone marrow","score":0.6510000228881836},{"id":"https://openalex.org/keywords/cell-seeding-efficiency","display_name":"cell seeding efficiency","score":0.7549999952316284},{"id":"https://openalex.org/keywords/adipogenic-differentiation","display_name":"adipogenesis","score":0.8410000205039978},{"id":"https://openalex.org/keywords/osteogenic-differentiation","display_name":"osteogenic differentiation","score":1.0},{"id":"https://openalex.org/keywords/clonogenicity","display_name":"clonogenicity","score":0.8949999809265137},{"id":"https://openalex.org/keywords/cd73","display_name":"CD73","score":0.5230000019073486},{"id":"https://openalex.org/keywords/cd90","display_name":"CD90","score":0.996999979019165},{"id":"https://openalex.org/keywords/endoglin","display_name":"CD105","score":0.9750000238418579},{"id":"https://openalex.org/keywords/regenerative-medicine","display_name":"regenerative medicine","score":0.8100000023841858}],"concepts":[{"id":"https://openalex.org/C176999471","wikidata":"https://www.wikidata.org/wiki/Q6839314","display_name":"Microcarrier","level":3,"score":0.9574193954467773},{"id":"https://openalex.org/C26291073","wikidata":"https://www.wikidata.org/wiki/Q310484","display_name":"Ex vivo","level":3,"score":0.848655104637146},{"id":"https://openalex.org/C198826908","wikidata":"https://www.wikidata.org/wiki/Q1922379","display_name":"Mesenchymal stem cell","level":2,"score":0.7040176391601562},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4703674018383026},{"id":"https://openalex.org/C28328180","wikidata":"https://www.wikidata.org/wiki/Q48196","display_name":"Stem cell","level":2,"score":0.4610089659690857},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.35956332087516785},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.3456355035305023},{"id":"https://openalex.org/C207001950","wikidata":"https://www.wikidata.org/wiki/Q141124","display_name":"In vivo","level":2,"score":0.3159663677215576},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.2609509527683258},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.20530754327774048},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.05883491039276123}],"mesh":[{"descriptor_ui":"D002452","descriptor_name":"Cell Count","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003470","descriptor_name":"Culture Media","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D018929","descriptor_name":"Cell Culture Techniques","qualifier_ui":"Q000295","qualifier_name":"instrumentation","is_major_topic":false},{"descriptor_ui":"D018929","descriptor_name":"Cell Culture Techniques","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D019149","descriptor_name":"Bioreactors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D055442","descriptor_name":"Metabolome","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D059630","descriptor_name":"Mesenchymal Stem Cells","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D059630","descriptor_name":"Mesenchymal Stem Cells","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1016/j.jbiotec.2010.02.015","doi":"https://doi.org/10.1016/j.jbiotec.2010.02.015","is_oa":false,"landing_page_url":"https://doi.org/10.1016/j.jbiotec.2010.02.015","pdf_url":null,"source":{"id":"https://openalex.org/S182099265","display_name":"Journal of Biotechnology","issn_l":"0168-1656","issn":["0168-1656","1873-4863"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-c","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biotechnology","raw_type":"journal-article"},{"id":"pmid:20188771","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/20188771","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of biotechnology","raw_type":"Journal Article"},{"id":"pmh:oai:hispana.mcu.es:58720285","doi":null,"is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.jbiotec.2010.02.015.","pdf_url":null,"source":{"id":"https://openalex.org/S4377196108","display_name":"Hispana","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"journal article"}],"best_oa_location":{"id":"pmh:oai:hispana.mcu.es:58720285","doi":null,"is_oa":true,"landing_page_url":"https://doi.org/10.1016/j.jbiotec.2010.02.015.","pdf_url":null,"source":{"id":"https://openalex.org/S4377196108","display_name":"Hispana","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"journal article"},"sustainable_development_goals":[{"score":0.4000000059604645,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":24,"referenced_works":["https://openalex.org/W1977689261","https://openalex.org/W1984180661","https://openalex.org/W1984652179","https://openalex.org/W1991867714","https://openalex.org/W2000721992","https://openalex.org/W2001895901","https://openalex.org/W2007383919","https://openalex.org/W2012814713","https://openalex.org/W2045456080","https://openalex.org/W2054814854","https://openalex.org/W2058387142","https://openalex.org/W2071458966","https://openalex.org/W2072047772","https://openalex.org/W2078266417","https://openalex.org/W2079246198","https://openalex.org/W2080762372","https://openalex.org/W2082781093","https://openalex.org/W2087578260","https://openalex.org/W2091895305","https://openalex.org/W2094421015","https://openalex.org/W2125066452","https://openalex.org/W2160542010","https://openalex.org/W2348145836","https://openalex.org/W4238425946"],"related_works":["https://openalex.org/W2034528239","https://openalex.org/W66030875","https://openalex.org/W1979187256","https://openalex.org/W1621262969","https://openalex.org/W1519416134","https://openalex.org/W2121494459","https://openalex.org/W2403109874","https://openalex.org/W2413550182","https://openalex.org/W7097661799","https://openalex.org/W2110278625"],"abstract_inverted_index":null,"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":8},{"year":2022,"cited_by_count":8},{"year":2021,"cited_by_count":7},{"year":2020,"cited_by_count":12},{"year":2019,"cited_by_count":9},{"year":2018,"cited_by_count":17},{"year":2017,"cited_by_count":15},{"year":2016,"cited_by_count":22},{"year":2015,"cited_by_count":9},{"year":2014,"cited_by_count":18},{"year":2013,"cited_by_count":18},{"year":2012,"cited_by_count":19}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}