{"id":"https://openalex.org/W2131114862","doi":"https://doi.org/10.1099/13500872-142-5-1097","title":"Application of a suite of 16S rRNA-specific oligonucleotide probes designed to investigate bacteria of the phylum cytophaga-flavobacter-bacteroides in the natural environment","display_name":"Application of a suite of 16S rRNA-specific oligonucleotide probes designed to investigate bacteria of the phylum cytophaga-flavobacter-bacteroides in the natural environment","publication_year":1996,"publication_date":"1996-05-01","ids":{"openalex":"https://openalex.org/W2131114862","doi":"https://doi.org/10.1099/13500872-142-5-1097","mag":"2131114862","pmid":"https://pubmed.ncbi.nlm.nih.gov/8704951"},"language":"en","primary_location":{"id":"doi:10.1099/13500872-142-5-1097","doi":"https://doi.org/10.1099/13500872-142-5-1097","is_oa":false,"landing_page_url":"https://doi.org/10.1099/13500872-142-5-1097","pdf_url":null,"source":{"id":"https://openalex.org/S196799759","display_name":"Microbiology","issn_l":"1350-0872","issn":["1350-0872","1465-2080"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320497","host_organization_name":"Microbiology Society","host_organization_lineage":["https://openalex.org/P4310320497"],"host_organization_lineage_names":["Microbiology Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microbiology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5090207525","display_name":"Werner Manz","orcid":"https://orcid.org/0000-0002-4320-4236","observed_orcids":["https://orcid.org/0000-0002-4320-4236"]},"institutions":[{"id":"https://openalex.org/I62916508","display_name":"Technical University of Munich","ror":"https://ror.org/02kkvpp62","country_code":"DE","type":"education","lineage":["https://openalex.org/I62916508"]}],"countries":["DE"],"is_corresponding":true,"raw_author_name":"Werner Manz","raw_affiliation_strings":["Lehrstuhl f\u00fcr Mikrobiologie, Technische Universit\u00e4t M\u00fcnchen, D-80290 M\u00fcnchen, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lehrstuhl f\u00fcr Mikrobiologie, Technische Universit\u00e4t M\u00fcnchen, D-80290 M\u00fcnchen, Germany","institution_ids":["https://openalex.org/I62916508"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5012288079","display_name":"Rudolf Amann","orcid":"https://orcid.org/0000-0002-0846-7372","observed_orcids":["https://orcid.org/0000-0002-0846-7372"]},"institutions":[{"id":"https://openalex.org/I62916508","display_name":"Technical University of Munich","ror":"https://ror.org/02kkvpp62","country_code":"DE","type":"education","lineage":["https://openalex.org/I62916508"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Rudolf Amann","raw_affiliation_strings":["Lehrstuhl f\u00fcr Mikrobiologie, Technische Universit\u00e4t M\u00fcnchen, D-80290 M\u00fcnchen, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lehrstuhl f\u00fcr Mikrobiologie, Technische Universit\u00e4t M\u00fcnchen, D-80290 M\u00fcnchen, Germany","institution_ids":["https://openalex.org/I62916508"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5027803715","display_name":"Wolfgang Ludwig","orcid":"https://orcid.org/0000-0002-3154-9114","observed_orcids":["https://orcid.org/0000-0002-3154-9114","https://orcid.org/0009-0004-5487-2645"]},"institutions":[{"id":"https://openalex.org/I62916508","display_name":"Technical University of Munich","ror":"https://ror.org/02kkvpp62","country_code":"DE","type":"education","lineage":["https://openalex.org/I62916508"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Wolfgang Ludwig","raw_affiliation_strings":["Lehrstuhl f\u00fcr Mikrobiologie, Technische Universit\u00e4t M\u00fcnchen, D-80290 M\u00fcnchen, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lehrstuhl f\u00fcr Mikrobiologie, Technische Universit\u00e4t M\u00fcnchen, D-80290 M\u00fcnchen, Germany","institution_ids":["https://openalex.org/I62916508"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5028851979","display_name":"Marc Vancanneyt","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I32597200","display_name":"Ghent University","ror":"https://ror.org/00cv9y106","country_code":"BE","type":"education","lineage":["https://openalex.org/I32597200"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"Marc Vancanneyt","raw_affiliation_strings":["Laboratorium voor Microbiologie, Universiteit Gent, Belgium"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratorium voor Microbiologie, Universiteit Gent, Belgium","institution_ids":["https://openalex.org/I32597200"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5009380156","display_name":"K. H. SCHLEIFER","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I62916508","display_name":"Technical University of Munich","ror":"https://ror.org/02kkvpp62","country_code":"DE","type":"education","lineage":["https://openalex.org/I62916508"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Karl-Heinz Schleifer","raw_affiliation_strings":["Lehrstuhl f\u00fcr Mikrobiologie, Technische Universit\u00e4t M\u00fcnchen, D-80290 M\u00fcnchen, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Lehrstuhl f\u00fcr Mikrobiologie, Technische Universit\u00e4t M\u00fcnchen, D-80290 M\u00fcnchen, Germany","institution_ids":["https://openalex.org/I62916508"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5090207525"],"corresponding_institution_ids":["https://openalex.org/I62916508"],"apc_list":null,"apc_paid":null,"fwci":24.9656,"has_fulltext":false,"cited_by_count":1178,"citation_normalized_percentile":{"value":0.99876419,"is_in_top_1_percent":true,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":97,"max":100},"biblio":{"volume":"142","issue":"5","first_page":"1097","last_page":"1106"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11791","display_name":"Microbial Community Ecology and Physiology","score":0.9204000234603882,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11791","display_name":"Microbial Community Ecology and Physiology","score":0.9204000234603882,"subfield":{"id":"https://openalex.org/subfields/2303","display_name":"Ecology"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10015","display_name":"Genomics and Phylogenetic Studies","score":0.032600000500679016,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10564","display_name":"Wastewater Treatment and Nitrogen Removal","score":0.02759999968111515,"subfield":{"id":"https://openalex.org/subfields/2310","display_name":"Pollution"},"field":{"id":"https://openalex.org/fields/23","display_name":"Environmental Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/porphyromonas","display_name":"Porphyromonas","score":0.8569999933242798},{"id":"https://openalex.org/keywords/prevotella","display_name":"Prevotella","score":0.824999988079071},{"id":"https://openalex.org/keywords/bacteroides","display_name":"Bacteroides","score":0.9990000128746033},{"id":"https://openalex.org/keywords/activated-sludge","display_name":"activated sludge","score":0.8090000152587891},{"id":"https://openalex.org/keywords/filamentous-bacteria","display_name":"filamentous bacteria","score":0.9599999785423279},{"id":"https://openalex.org/keywords/bacterial-community-composition","display_name":"bacterial community composition","score":0.7409999966621399},{"id":"https://openalex.org/keywords/in-situ-hybridization","display_name":"in situ hybridization","score":0.8240000009536743},{"id":"https://openalex.org/keywords/fluorescent-probes","display_name":"fluorescent probes","score":0.46700000762939453}],"concepts":[{"id":"https://openalex.org/C91066513","wikidata":"https://www.wikidata.org/wiki/Q38348","display_name":"Phylum","level":3,"score":0.7506632804870605},{"id":"https://openalex.org/C2778098358","wikidata":"https://www.wikidata.org/wiki/Q3009545","display_name":"Cytophaga","level":5,"score":0.7093320488929749},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.6172510385513306},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.596220076084137},{"id":"https://openalex.org/C2778168646","wikidata":"https://www.wikidata.org/wiki/Q133186","display_name":"Bacteroides","level":3,"score":0.5940302014350891},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.577782928943634},{"id":"https://openalex.org/C2780246119","wikidata":"https://www.wikidata.org/wiki/Q221817","display_name":"Bacteroides fragilis","level":3,"score":0.5191234946250916},{"id":"https://openalex.org/C42062724","wikidata":"https://www.wikidata.org/wiki/Q1209205","display_name":"16S ribosomal RNA","level":3,"score":0.5060545802116394},{"id":"https://openalex.org/C501593827","wikidata":"https://www.wikidata.org/wiki/Q12187","display_name":"Antibiotics","level":2,"score":0.24163514375686646},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.07291242480278015},{"id":"https://openalex.org/C2778106697","wikidata":"https://www.wikidata.org/wiki/Q140572","display_name":"Flavobacterium","level":4,"score":0.0},{"id":"https://openalex.org/C2781463711","wikidata":"https://www.wikidata.org/wiki/Q131143","display_name":"Pseudomonas","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D001439","descriptor_name":"Bacteroides","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D001439","descriptor_name":"Bacteroides","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003589","descriptor_name":"Cytophaga","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D003589","descriptor_name":"Cytophaga","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D004783","descriptor_name":"Environmental Microbiology","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D005243","descriptor_name":"Feces","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":false},{"descriptor_ui":"D005417","descriptor_name":"Flavobacterium","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D005417","descriptor_name":"Flavobacterium","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D006090","descriptor_name":"Gram-Negative Bacteria","qualifier_ui":"Q000145","qualifier_name":"classification","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010802","descriptor_name":"Phylogeny","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012329","descriptor_name":"RNA, Bacterial","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D012336","descriptor_name":"RNA, Ribosomal, 16S","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D015345","descriptor_name":"Oligonucleotide Probes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1099/13500872-142-5-1097","doi":"https://doi.org/10.1099/13500872-142-5-1097","is_oa":false,"landing_page_url":"https://doi.org/10.1099/13500872-142-5-1097","pdf_url":null,"source":{"id":"https://openalex.org/S196799759","display_name":"Microbiology","issn_l":"1350-0872","issn":["1350-0872","1465-2080"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320497","host_organization_name":"Microbiology Society","host_organization_lineage":["https://openalex.org/P4310320497"],"host_organization_lineage_names":["Microbiology Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microbiology","raw_type":"journal-article"},{"id":"pmid:8704951","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8704951","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microbiology (Reading, England)","raw_type":"Comparative Study"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.864.610","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.864.610","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://www.microbiologyresearch.org/docserver/fulltext/micro/142/5/mic-142-5-1097.pdf?accname%3Dsgid026644%26checksum%3D7E986CC1B977A0BB4636FB0991425020%26expires%3D1462788797%26id%3Did","raw_type":"text"},{"id":"pmh:oai:archive.ugent.be:188191","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/1854/LU-188191","pdf_url":null,"source":{"id":"https://openalex.org/S4306400478","display_name":"Ghent University Academic Bibliography (Ghent University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I32597200","host_organization_name":"Ghent University","host_organization_lineage":["https://openalex.org/I32597200"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"ISSN: 1350-0872","raw_type":"info:eu-repo/semantics/article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":48,"referenced_works":["https://openalex.org/W995695737","https://openalex.org/W1501270673","https://openalex.org/W1501872728","https://openalex.org/W1509305848","https://openalex.org/W1536048645","https://openalex.org/W1543348742","https://openalex.org/W1547852780","https://openalex.org/W1576056687","https://openalex.org/W1701869895","https://openalex.org/W1770972723","https://openalex.org/W1777269504","https://openalex.org/W1785423892","https://openalex.org/W1848498826","https://openalex.org/W1937000379","https://openalex.org/W1971017825","https://openalex.org/W1973241521","https://openalex.org/W1978209538","https://openalex.org/W1986061526","https://openalex.org/W1987344200","https://openalex.org/W1990416781","https://openalex.org/W2002525584","https://openalex.org/W2006825775","https://openalex.org/W2011079885","https://openalex.org/W2011349350","https://openalex.org/W2013722028","https://openalex.org/W2021544133","https://openalex.org/W2043842862","https://openalex.org/W2044294290","https://openalex.org/W2047396516","https://openalex.org/W2065975356","https://openalex.org/W2066109362","https://openalex.org/W2069897169","https://openalex.org/W2090496334","https://openalex.org/W2150565043","https://openalex.org/W2154693275","https://openalex.org/W2154989857","https://openalex.org/W2171205176","https://openalex.org/W2172521887","https://openalex.org/W2212856635","https://openalex.org/W2307521299","https://openalex.org/W2343892901","https://openalex.org/W3140104194","https://openalex.org/W3142525956","https://openalex.org/W4205634428","https://openalex.org/W4211022623","https://openalex.org/W4298161061","https://openalex.org/W7024896502","https://openalex.org/W7028693473"],"related_works":["https://openalex.org/W1965457385","https://openalex.org/W7099078834","https://openalex.org/W2167730457","https://openalex.org/W2089929496","https://openalex.org/W2088284295","https://openalex.org/W33527881","https://openalex.org/W2135256281","https://openalex.org/W2531529354","https://openalex.org/W2803940010","https://openalex.org/W1973516153"],"abstract_inverted_index":{"We":[0],"designed":[1,66],"a":[2,38],"panel":[3],"of":[4,13,26,96,104,132,145,151,156,161,177],"four":[5],"16S":[6,77],"rRNA-targeted":[7],"oligonucleotide":[8],"probes":[9,64,109,116],"specific":[10],"for":[11,42,57],"bacteria":[12],"the":[14,27,31,43,49,97,119,133,162,178],"phylum":[15,99],"cytophaga-flavobacter-bacteroides":[16],"(CFB).":[17],"Probes":[18],"CF319a":[19],"and":[20,30,46,71,78,89,100,121,148,165],"CF319b":[21],"are":[22],"targeted":[23],"to":[24,73,117],"members":[25,131,160],"flavobacteria-cytophaga":[28,134],"group":[29,135],"genus":[32],"Porphyromonas,":[33],"whereas":[34],"probe":[35,53,158],"BAC303":[36],"has":[37],"target":[39],"region":[40],"characteristic":[41,149],"genera":[44,163],"Prevotella":[45,166],"Bacteroides":[47,164],"within":[48],"bacteroides":[50],"group.":[51],"The":[52,82,106],"FFE8b":[54],"was":[55],"developed":[56,108],"species-specific":[58],"hybridizations":[59],"with":[60,113],"Flavobacterium":[61],"ferrugineum.":[62],"All":[63],"were":[65,84,110,136],"by":[67,87,138],"computer-assisted":[68],"sequence":[69],"analysis":[70],"compared":[72],"all":[74],"currently":[75],"accessible":[76],"23S":[79],"rRNA":[80],"sequences.":[81],"oligonucleotides":[83],"further":[85],"evaluated":[86],"whole-cell":[88],"non-radioactive":[90],"dot-blot":[91],"hybridization":[92,141],"against":[93],"reference":[94],"strains":[95],"CFB":[98],"other":[101,114],"major":[102],"lineages":[103],"Bacteria.":[105],"newly":[107],"used":[111],"together":[112],"higher-order":[115],"analyse":[118],"structure":[120],"community":[122],"composition":[123],"in":[124,139],"complex":[125],"environments.":[126],"In":[127],"activated":[128],"sludge":[129,146],"samples,":[130],"revealed":[137],"situ":[140],"as":[142,173],"important":[143,175],"constituents":[144],"flocs":[147],"colonizers":[150],"filamentous":[152],"bacteria.":[153],"By":[154],"application":[155],"fluorescent":[157],"BAC303,":[159],"could":[167],"be":[168],"visualized":[169],"without":[170],"prior":[171],"cultivation":[172],"an":[174],"part":[176],"human":[179],"faecal":[180],"microflora.":[181]},"counts_by_year":[{"year":2026,"cited_by_count":5},{"year":2025,"cited_by_count":5},{"year":2024,"cited_by_count":20},{"year":2023,"cited_by_count":19},{"year":2022,"cited_by_count":21},{"year":2021,"cited_by_count":27},{"year":2020,"cited_by_count":21},{"year":2019,"cited_by_count":28},{"year":2018,"cited_by_count":23},{"year":2017,"cited_by_count":29},{"year":2016,"cited_by_count":38},{"year":2015,"cited_by_count":42},{"year":2014,"cited_by_count":43},{"year":2013,"cited_by_count":54},{"year":2012,"cited_by_count":60}],"updated_date":"2026-11-13T13:42:25.865203","created_date":"2016-06-24T00:00:00"}