{"id":"https://openalex.org/W2129658913","doi":"https://doi.org/10.1002/bit.20326","title":"Engineering Chinese hamster ovary cells to maximize effector function of produced antibodies using FUT8 siRNA","display_name":"Engineering Chinese hamster ovary cells to maximize effector function of produced antibodies using FUT8 siRNA","publication_year":2004,"publication_date":"2004-10-28","ids":{"openalex":"https://openalex.org/W2129658913","doi":"https://doi.org/10.1002/bit.20326","mag":"2129658913","pmid":"https://pubmed.ncbi.nlm.nih.gov/15515168"},"language":"en","primary_location":{"id":"doi:10.1002/bit.20326","doi":"https://doi.org/10.1002/bit.20326","is_oa":false,"landing_page_url":"https://doi.org/10.1002/bit.20326","pdf_url":null,"source":{"id":"https://openalex.org/S151564723","display_name":"Biotechnology and Bioengineering","issn_l":"0006-3592","issn":["0006-3592","1097-0290"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Bioengineering","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5111804296","display_name":"Katsuhiro Mori","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I205823427","display_name":"Kyowa Kirin (Japan)","ror":"https://ror.org/000wej815","country_code":"JP","type":"company","lineage":["https://openalex.org/I205823427"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Katsuhiro Mori","raw_affiliation_strings":["Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan","fax: + 81-42-726-8330","telephone: + 81-42-725-2555"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan","institution_ids":["https://openalex.org/I205823427"]},{"raw_affiliation_string":"fax: + 81-42-726-8330","institution_ids":[]},{"raw_affiliation_string":"telephone: + 81-42-725-2555","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5047833798","display_name":"Reiko Kuni\u2010Kamochi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I205823427","display_name":"Kyowa Kirin (Japan)","ror":"https://ror.org/000wej815","country_code":"JP","type":"company","lineage":["https://openalex.org/I205823427"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Reiko Kuni\u2010Kamochi","raw_affiliation_strings":["Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan; telephone: + 81-42-725-2555; fax: + 81-42-726-8330","Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan","fax: + 81-42-726-8330","telephone: + 81-42-725-2555"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan; telephone: + 81-42-725-2555; fax: + 81-42-726-8330","institution_ids":[]},{"raw_affiliation_string":"Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan","institution_ids":["https://openalex.org/I205823427"]},{"raw_affiliation_string":"fax: + 81-42-726-8330","institution_ids":[]},{"raw_affiliation_string":"telephone: + 81-42-725-2555","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5052867587","display_name":"Naoko Yamane\u2010Ohnuki","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I205823427","display_name":"Kyowa Kirin (Japan)","ror":"https://ror.org/000wej815","country_code":"JP","type":"company","lineage":["https://openalex.org/I205823427"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Naoko Yamane\u2010Ohnuki","raw_affiliation_strings":["Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan; telephone: + 81-42-725-2555; fax: + 81-42-726-8330","Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan","fax: + 81-42-726-8330","telephone: + 81-42-725-2555"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan; telephone: + 81-42-725-2555; fax: + 81-42-726-8330","institution_ids":[]},{"raw_affiliation_string":"Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan","institution_ids":["https://openalex.org/I205823427"]},{"raw_affiliation_string":"fax: + 81-42-726-8330","institution_ids":[]},{"raw_affiliation_string":"telephone: + 81-42-725-2555","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5070323911","display_name":"Masako Wakitani","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I205823427","display_name":"Kyowa Kirin (Japan)","ror":"https://ror.org/000wej815","country_code":"JP","type":"company","lineage":["https://openalex.org/I205823427"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Masako Wakitani","raw_affiliation_strings":["Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan; telephone: + 81-42-725-2555; fax: + 81-42-726-8330","Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan","fax: + 81-42-726-8330","telephone: + 81-42-725-2555"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan; telephone: + 81-42-725-2555; fax: + 81-42-726-8330","institution_ids":[]},{"raw_affiliation_string":"Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan","institution_ids":["https://openalex.org/I205823427"]},{"raw_affiliation_string":"fax: + 81-42-726-8330","institution_ids":[]},{"raw_affiliation_string":"telephone: + 81-42-725-2555","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5078624659","display_name":"Kazuya Yamano","orcid":"https://orcid.org/0000-0003-0693-486X","observed_orcids":["https://orcid.org/0000-0003-0693-486X"]},"institutions":[{"id":"https://openalex.org/I205823427","display_name":"Kyowa Kirin (Japan)","ror":"https://ror.org/000wej815","country_code":"JP","type":"company","lineage":["https://openalex.org/I205823427"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Kazuya Yamano","raw_affiliation_strings":["Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan; telephone: + 81-42-725-2555; fax: + 81-42-726-8330","Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan","fax: + 81-42-726-8330","telephone: + 81-42-725-2555"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan; telephone: + 81-42-725-2555; fax: + 81-42-726-8330","institution_ids":[]},{"raw_affiliation_string":"Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan","institution_ids":["https://openalex.org/I205823427"]},{"raw_affiliation_string":"fax: + 81-42-726-8330","institution_ids":[]},{"raw_affiliation_string":"telephone: + 81-42-725-2555","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5085006609","display_name":"Harue Imai","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I205823427","display_name":"Kyowa Kirin (Japan)","ror":"https://ror.org/000wej815","country_code":"JP","type":"company","lineage":["https://openalex.org/I205823427"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Harue Imai","raw_affiliation_strings":["Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan; telephone: + 81-42-725-2555; fax: + 81-42-726-8330","Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan","fax: + 81-42-726-8330","telephone: + 81-42-725-2555"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan; telephone: + 81-42-725-2555; fax: + 81-42-726-8330","institution_ids":[]},{"raw_affiliation_string":"Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan","institution_ids":["https://openalex.org/I205823427"]},{"raw_affiliation_string":"fax: + 81-42-726-8330","institution_ids":[]},{"raw_affiliation_string":"telephone: + 81-42-725-2555","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5057240756","display_name":"Yutaka Kanda","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I205823427","display_name":"Kyowa Kirin (Japan)","ror":"https://ror.org/000wej815","country_code":"JP","type":"company","lineage":["https://openalex.org/I205823427"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Yutaka Kanda","raw_affiliation_strings":["Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan; telephone: + 81-42-725-2555; fax: + 81-42-726-8330","Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan","fax: + 81-42-726-8330","telephone: + 81-42-725-2555"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan; telephone: + 81-42-725-2555; fax: + 81-42-726-8330","institution_ids":[]},{"raw_affiliation_string":"Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan","institution_ids":["https://openalex.org/I205823427"]},{"raw_affiliation_string":"fax: + 81-42-726-8330","institution_ids":[]},{"raw_affiliation_string":"telephone: + 81-42-725-2555","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5044584457","display_name":"Rinpei Niwa","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I205823427","display_name":"Kyowa Kirin (Japan)","ror":"https://ror.org/000wej815","country_code":"JP","type":"company","lineage":["https://openalex.org/I205823427"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Rinpei Niwa","raw_affiliation_strings":["Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan; telephone: + 81-42-725-2555; fax: + 81-42-726-8330","Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan","fax: + 81-42-726-8330","telephone: + 81-42-725-2555"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan; telephone: + 81-42-725-2555; fax: + 81-42-726-8330","institution_ids":[]},{"raw_affiliation_string":"Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan","institution_ids":["https://openalex.org/I205823427"]},{"raw_affiliation_string":"fax: + 81-42-726-8330","institution_ids":[]},{"raw_affiliation_string":"telephone: + 81-42-725-2555","institution_ids":[]}]},{"author_position":"middle","author":{"id":null,"display_name":"Shigeru Iida","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I205823427","display_name":"Kyowa Kirin (Japan)","ror":"https://ror.org/000wej815","country_code":"JP","type":"company","lineage":["https://openalex.org/I205823427"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Shigeru Iida","raw_affiliation_strings":["Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan; telephone: + 81-42-725-2555; fax: + 81-42-726-8330","Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan","fax: + 81-42-726-8330","telephone: + 81-42-725-2555"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan; telephone: + 81-42-725-2555; fax: + 81-42-726-8330","institution_ids":[]},{"raw_affiliation_string":"Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan","institution_ids":["https://openalex.org/I205823427"]},{"raw_affiliation_string":"fax: + 81-42-726-8330","institution_ids":[]},{"raw_affiliation_string":"telephone: + 81-42-725-2555","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5022317932","display_name":"Kazuhisa Uchida","orcid":"https://orcid.org/0000-0001-7360-4356","observed_orcids":["https://orcid.org/0000-0001-7360-4356"]},"institutions":[{"id":"https://openalex.org/I205823427","display_name":"Kyowa Kirin (Japan)","ror":"https://ror.org/000wej815","country_code":"JP","type":"company","lineage":["https://openalex.org/I205823427"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Kazuhisa Uchida","raw_affiliation_strings":["Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan; telephone: + 81-42-725-2555; fax: + 81-42-726-8330","Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan","fax: + 81-42-726-8330","telephone: + 81-42-725-2555"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan; telephone: + 81-42-725-2555; fax: + 81-42-726-8330","institution_ids":[]},{"raw_affiliation_string":"Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan","institution_ids":["https://openalex.org/I205823427"]},{"raw_affiliation_string":"fax: + 81-42-726-8330","institution_ids":[]},{"raw_affiliation_string":"telephone: + 81-42-725-2555","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109957616","display_name":"Kenya Shitara","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I205823427","display_name":"Kyowa Kirin (Japan)","ror":"https://ror.org/000wej815","country_code":"JP","type":"company","lineage":["https://openalex.org/I205823427"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Kenya Shitara","raw_affiliation_strings":["Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan; telephone: + 81-42-725-2555; fax: + 81-42-726-8330","Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan","fax: + 81-42-726-8330","telephone: + 81-42-725-2555"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan; telephone: + 81-42-725-2555; fax: + 81-42-726-8330","institution_ids":[]},{"raw_affiliation_string":"Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan","institution_ids":["https://openalex.org/I205823427"]},{"raw_affiliation_string":"fax: + 81-42-726-8330","institution_ids":[]},{"raw_affiliation_string":"telephone: + 81-42-725-2555","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5085088469","display_name":"Mitsuo Satoh","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I205823427","display_name":"Kyowa Kirin (Japan)","ror":"https://ror.org/000wej815","country_code":"JP","type":"company","lineage":["https://openalex.org/I205823427"]}],"countries":["JP"],"is_corresponding":true,"raw_author_name":"Mitsuo Satoh","raw_affiliation_strings":["Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan; telephone: + 81-42-725-2555; fax: + 81-42-726-8330","Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan","fax: + 81-42-726-8330","telephone: + 81-42-725-2555"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan; telephone: + 81-42-725-2555; fax: + 81-42-726-8330","institution_ids":[]},{"raw_affiliation_string":"Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan","institution_ids":["https://openalex.org/I205823427"]},{"raw_affiliation_string":"fax: + 81-42-726-8330","institution_ids":[]},{"raw_affiliation_string":"telephone: + 81-42-725-2555","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5085088469"],"corresponding_institution_ids":["https://openalex.org/I205823427"],"apc_list":{"value":5060,"currency":"USD","value_usd":5060},"apc_paid":null,"fwci":3.0255,"has_fulltext":false,"cited_by_count":174,"citation_normalized_percentile":{"value":0.92629071,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":89,"max":100},"biblio":{"volume":"88","issue":"7","first_page":"901","last_page":"908"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.9922000169754028,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11663","display_name":"Viral Infectious Diseases and Gene Expression in Insects","score":0.9922000169754028,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11016","display_name":"Monoclonal and Polyclonal Antibodies Research","score":0.004800000227987766,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10602","display_name":"Glycosylation and Glycoproteins Research","score":0.0008999999845400453,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/alpha1-6-fucosyltransferase","display_name":"alpha-1,6-fucosyltransferase","score":0.9890000224113464},{"id":"https://openalex.org/keywords/antibody-dependent-cellular-cytotoxicity","display_name":"antibody-dependent cellular cytotoxicity","score":0.9409999847412109},{"id":"https://openalex.org/keywords/cho-dg44-cells","display_name":"CHO DG44 cells","score":0.7919999957084656},{"id":"https://openalex.org/keywords/oligosaccharide-structure","display_name":"oligosaccharide structure","score":0.7609999775886536},{"id":"https://openalex.org/keywords/sirna-knockdown","display_name":"siRNA knockdown","score":0.5519999861717224}],"concepts":[{"id":"https://openalex.org/C175656101","wikidata":"https://www.wikidata.org/wiki/Q56646","display_name":"Chinese hamster ovary cell","level":3,"score":0.9545459747314453},{"id":"https://openalex.org/C40677261","wikidata":"https://www.wikidata.org/wiki/Q21418091","display_name":"Antibody-dependent cell-mediated cytotoxicity","level":4,"score":0.7719218730926514},{"id":"https://openalex.org/C159654299","wikidata":"https://www.wikidata.org/wiki/Q79460","display_name":"Antibody","level":2,"score":0.7436279058456421},{"id":"https://openalex.org/C22615655","wikidata":"https://www.wikidata.org/wiki/Q203221","display_name":"Small interfering RNA","level":4,"score":0.6239281892776489},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.6149487495422363},{"id":"https://openalex.org/C2777762822","wikidata":"https://www.wikidata.org/wiki/Q5507035","display_name":"Fucosyltransferase","level":3,"score":0.5655030608177185},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.5570173859596252},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.48543912172317505},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4789203405380249},{"id":"https://openalex.org/C207545495","wikidata":"https://www.wikidata.org/wiki/Q2573513","display_name":"Agglutinin","level":3,"score":0.4452967941761017},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.4397217631340027},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4012705087661743},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2354421317577362},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.2014646828174591},{"id":"https://openalex.org/C542903549","wikidata":"https://www.wikidata.org/wiki/Q422248","display_name":"Monoclonal antibody","level":3,"score":0.1407451331615448},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.1048828661441803},{"id":"https://openalex.org/C2780758401","wikidata":"https://www.wikidata.org/wiki/Q408693","display_name":"Lectin","level":2,"score":0.0853656530380249},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000911","descriptor_name":"Antibodies, Monoclonal","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D000911","descriptor_name":"Antibodies, Monoclonal","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D003412","descriptor_name":"Cricetulus","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005647","descriptor_name":"Fucosyltransferases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D005647","descriptor_name":"Fucosyltransferases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006224","descriptor_name":"Cricetinae","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015202","descriptor_name":"Protein Engineering","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D016466","descriptor_name":"CHO Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020868","descriptor_name":"Gene Silencing","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D024861","descriptor_name":"Genetic Enhancement","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D034741","descriptor_name":"RNA, Small Interfering","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1002/bit.20326","doi":"https://doi.org/10.1002/bit.20326","is_oa":false,"landing_page_url":"https://doi.org/10.1002/bit.20326","pdf_url":null,"source":{"id":"https://openalex.org/S151564723","display_name":"Biotechnology and Bioengineering","issn_l":"0006-3592","issn":["0006-3592","1097-0290"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Bioengineering","raw_type":"journal-article"},{"id":"pmid:15515168","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/15515168","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and bioengineering","raw_type":"Comparative Study"}],"best_oa_location":null,"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.8371999859809875,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":36,"referenced_works":["https://openalex.org/W97178176","https://openalex.org/W1493303756","https://openalex.org/W1518819083","https://openalex.org/W1528342365","https://openalex.org/W1611419331","https://openalex.org/W1819720416","https://openalex.org/W1997422287","https://openalex.org/W1997913594","https://openalex.org/W2001451148","https://openalex.org/W2002615250","https://openalex.org/W2012188597","https://openalex.org/W2017642141","https://openalex.org/W2022727563","https://openalex.org/W2038783205","https://openalex.org/W2039127825","https://openalex.org/W2044360617","https://openalex.org/W2051645590","https://openalex.org/W2055503849","https://openalex.org/W2059347528","https://openalex.org/W2063840206","https://openalex.org/W2064559284","https://openalex.org/W2076445790","https://openalex.org/W2081867678","https://openalex.org/W2082117763","https://openalex.org/W2084136419","https://openalex.org/W2087881430","https://openalex.org/W2098100188","https://openalex.org/W2101287056","https://openalex.org/W2113657260","https://openalex.org/W2115571948","https://openalex.org/W2128402413","https://openalex.org/W2133626640","https://openalex.org/W2134074192","https://openalex.org/W2137354517","https://openalex.org/W2151922790","https://openalex.org/W4294605288"],"related_works":["https://openalex.org/W2036928962","https://openalex.org/W2115571948","https://openalex.org/W2136722681","https://openalex.org/W2591436500","https://openalex.org/W4389433823","https://openalex.org/W2168072557","https://openalex.org/W4229056060","https://openalex.org/W2123847878","https://openalex.org/W2528678937","https://openalex.org/W3047044926"],"abstract_inverted_index":{"We":[0,34],"explored":[1],"the":[2,64,87,94,117],"possibility":[3],"of":[4,25,63,120],"converting":[5],"established":[6],"antibody-producing":[7],"cells":[8,10,89,126],"to":[9,44,83,127],"producing":[11,71,99],"high":[12],"antibody-dependent":[13],"cellular":[14],"cytotoxicity":[15],"(ADCC)":[16],"antibodies.":[17],"The":[18],"conversion":[19],"was":[20],"made":[21],"by":[22,86,124],"constitutive":[23,111],"expression":[24,43,67,114],"small":[26],"interfering":[27],"RNA":[28],"(siRNA)":[29],"against":[30],"alpha1,6":[31],"fucosyltransferase":[32],"(FUT8).":[33],"found":[35],"two":[36],"effective":[37],"siRNAs,":[38],"which":[39],"reduce":[40],"FUT8":[41,65,112],"mRNA":[42],"20%":[45],"when":[46],"introduced":[47],"into":[48],"Chinese":[49],"hamster":[50],"ovary":[51],"(CHO)/DG44":[52],"cells.":[53],"Selection":[54],"for":[55],"Lens":[56],"culinaris":[57],"agglutinin":[58],"(LCA)-resistant":[59],"clones":[60,70,96],"after":[61],"introduction":[62],"siRNA":[66,113],"plasmids":[68],"yields":[69],"highly":[72],"defucosylated":[73,100],"(approximately":[74,90],"60%)":[75],"antibody":[76,84,101,122],"with":[77,130],"over":[78],"100-fold":[79],"higher":[80],"ADCC":[81],"compared":[82],"produced":[85,123],"parental":[88],"10%":[91],"defucosylated).":[92],"Moreover,":[93],"selected":[95],"remain":[97],"stable,":[98],"even":[102],"in":[103],"serum-free":[104],"fed-batch":[105],"culture.":[106],"Our":[107],"results":[108],"demonstrate":[109],"that":[110],"can":[115],"control":[116],"oligosaccharide":[118],"structure":[119],"recombinant":[121],"CHO":[125],"yield":[128],"antibodies":[129],"dramatically":[131],"enhanced":[132],"ADCC.":[133]},"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":4},{"year":2022,"cited_by_count":6},{"year":2021,"cited_by_count":5},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":7},{"year":2018,"cited_by_count":15},{"year":2017,"cited_by_count":12},{"year":2016,"cited_by_count":10},{"year":2015,"cited_by_count":12},{"year":2014,"cited_by_count":13},{"year":2013,"cited_by_count":6},{"year":2012,"cited_by_count":7}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}