{"id":"https://openalex.org/W2129138696","doi":"https://doi.org/10.1074/jbc.m401928200","title":"Characterization of Human UDP-glucose Dehydrogenase","display_name":"Characterization of Human UDP-glucose Dehydrogenase","publication_year":2004,"publication_date":"2004-05-01","ids":{"openalex":"https://openalex.org/W2129138696","doi":"https://doi.org/10.1074/jbc.m401928200","mag":"2129138696","pmid":"https://pubmed.ncbi.nlm.nih.gov/15044486"},"language":"en","primary_location":{"id":"doi:10.1074/jbc.m401928200","doi":"https://doi.org/10.1074/jbc.m401928200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m401928200","pdf_url":"http://www.jbc.org/article/S0021925820667345/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"http://www.jbc.org/article/S0021925820667345/pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5017159118","display_name":"Brandi J. Sommer","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I114395901","display_name":"University of Nebraska\u2013Lincoln","ror":"https://ror.org/043mer456","country_code":"US","type":"education","lineage":["https://openalex.org/I114395901"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Brandi J. Sommer","raw_affiliation_strings":["Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588-0664, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588-0664, USA","institution_ids":["https://openalex.org/I114395901"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5046465352","display_name":"Joseph J. Barycki","orcid":"https://orcid.org/0000-0002-2363-0617","observed_orcids":["https://orcid.org/0000-0002-2363-0617"]},"institutions":[{"id":"https://openalex.org/I114395901","display_name":"University of Nebraska\u2013Lincoln","ror":"https://ror.org/043mer456","country_code":"US","type":"education","lineage":["https://openalex.org/I114395901"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Joseph J. Barycki","raw_affiliation_strings":["Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588-0664"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588-0664","institution_ids":["https://openalex.org/I114395901"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5102936566","display_name":"Melanie A. Simpson","orcid":"https://orcid.org/0000-0003-2481-2129","observed_orcids":["https://orcid.org/0000-0003-2481-2129"]},"institutions":[{"id":"https://openalex.org/I114395901","display_name":"University of Nebraska\u2013Lincoln","ror":"https://ror.org/043mer456","country_code":"US","type":"education","lineage":["https://openalex.org/I114395901"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Melanie A. Simpson","raw_affiliation_strings":["Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588-0664"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, University of Nebraska, Lincoln, Nebraska 68588-0664","institution_ids":["https://openalex.org/I114395901"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5102936566"],"corresponding_institution_ids":["https://openalex.org/I114395901"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":0.887,"has_fulltext":true,"cited_by_count":61,"citation_normalized_percentile":{"value":0.70325363,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"279","issue":"22","first_page":"23590","last_page":"23596"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.4027999937534332,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.4027999937534332,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12827","display_name":"Biochemical and Molecular Research","score":0.18930000066757202,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12191","display_name":"Physical Organic Chemistry and Reaction Kinetics","score":0.0771000012755394,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/udp-glucose-dehydrogenase","display_name":"UDP-glucose dehydrogenase","score":0.9990000128746033},{"id":"https://openalex.org/keywords/udp-glucuronic-acid","display_name":"UDP-glucuronic acid","score":0.9520000219345093},{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.5870000123977661},{"id":"https://openalex.org/keywords/catalytic-residues","display_name":"catalytic residues","score":0.7160000205039978},{"id":"https://openalex.org/keywords/point-mutants","display_name":"point mutants","score":0.6359999775886536},{"id":"https://openalex.org/keywords/enzyme-inhibition","display_name":"enzyme inhibition","score":0.42800000309944153},{"id":"https://openalex.org/keywords/nad-cofactor","display_name":"NAD+ cofactor","score":0.6629999876022339},{"id":"https://openalex.org/keywords/active-site-model","display_name":"active site model","score":0.6669999957084656},{"id":"https://openalex.org/keywords/streptococcus-pyogenes","display_name":"Streptococcus pyogenes","score":0.9150000214576721}],"concepts":[{"id":"https://openalex.org/C41183919","wikidata":"https://www.wikidata.org/wiki/Q423026","display_name":"Active site","level":3,"score":0.6746760010719299},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.6633827686309814},{"id":"https://openalex.org/C2776317432","wikidata":"https://www.wikidata.org/wiki/Q412547","display_name":"Dehydrogenase","level":3,"score":0.6124500036239624},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6038640737533569},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.547836184501648},{"id":"https://openalex.org/C75520062","wikidata":"https://www.wikidata.org/wiki/Q15321392","display_name":"NAD+ kinase","level":3,"score":0.46936777234077454},{"id":"https://openalex.org/C197957613","wikidata":"https://www.wikidata.org/wiki/Q407280","display_name":"Cofactor","level":3,"score":0.4680231511592865},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.4555879235267639},{"id":"https://openalex.org/C2776414213","wikidata":"https://www.wikidata.org/wiki/Q183290","display_name":"Serine","level":3,"score":0.44750651717185974}],"mesh":[{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003545","descriptor_name":"Cysteine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008239","descriptor_name":"Lysine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010084","descriptor_name":"Oxidation-Reduction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011487","descriptor_name":"Protein Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013297","descriptor_name":"Streptococcus pyogenes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013329","descriptor_name":"Structure-Activity Relationship","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014533","descriptor_name":"Uridine Diphosphate Glucose Dehydrogenase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D014533","descriptor_name":"Uridine Diphosphate Glucose Dehydrogenase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014533","descriptor_name":"Uridine Diphosphate Glucose Dehydrogenase","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D020134","descriptor_name":"Catalytic Domain","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1074/jbc.m401928200","doi":"https://doi.org/10.1074/jbc.m401928200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m401928200","pdf_url":"http://www.jbc.org/article/S0021925820667345/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:15044486","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/15044486","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"},{"id":"pmh:oai:digitalcommons.unl.edu:biochemfacpub-1012","doi":null,"is_oa":true,"landing_page_url":"https://digitalcommons.unl.edu/biochemfacpub/12","pdf_url":"https://digitalcommons.unl.edu/biochemfacpub/12","source":{"id":"https://openalex.org/S4306400577","display_name":"Lincoln (University of Nebraska)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I114395901","host_organization_name":"University of Nebraska\u2013Lincoln","host_organization_lineage":["https://openalex.org/I114395901"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Department of Biochemistry: Faculty Publications","raw_type":"text"}],"best_oa_location":{"id":"doi:10.1074/jbc.m401928200","doi":"https://doi.org/10.1074/jbc.m401928200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m401928200","pdf_url":"http://www.jbc.org/article/S0021925820667345/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.4000000059604645,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G6948705967","display_name":"Nebraska Center for Cellular Signaling","funder_award_id":"P20RR018759","funder_id":"https://openalex.org/F4320337375","funder_display_name":"National Center for Research Resources"}],"funders":[{"id":"https://openalex.org/F4320309137","display_name":"University of Nebraska Medical Center","ror":"https://ror.org/00thqtb16"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337375","display_name":"National Center for Research Resources","ror":"https://ror.org/04pw6fb54"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2129138696.pdf","grobid_xml":"https://content.openalex.org/works/W2129138696.grobid-xml"},"referenced_works_count":27,"referenced_works":["https://openalex.org/W1479759526","https://openalex.org/W1590379492","https://openalex.org/W1969670626","https://openalex.org/W1974629826","https://openalex.org/W1994158354","https://openalex.org/W1996104964","https://openalex.org/W1997124026","https://openalex.org/W2003199291","https://openalex.org/W2017319729","https://openalex.org/W2019204518","https://openalex.org/W2030605936","https://openalex.org/W2051393857","https://openalex.org/W2069685752","https://openalex.org/W2072906796","https://openalex.org/W2074140716","https://openalex.org/W2077007440","https://openalex.org/W2084466582","https://openalex.org/W2092687582","https://openalex.org/W2108504660","https://openalex.org/W2113780941","https://openalex.org/W2128466784","https://openalex.org/W2139966914","https://openalex.org/W2144234813","https://openalex.org/W2150222955","https://openalex.org/W3138259897","https://openalex.org/W4245537525","https://openalex.org/W4255328866"],"related_works":["https://openalex.org/W1994051122","https://openalex.org/W2291453607","https://openalex.org/W1572457727","https://openalex.org/W7096884238","https://openalex.org/W2336713500","https://openalex.org/W2085347056","https://openalex.org/W18314362","https://openalex.org/W1982819338","https://openalex.org/W2032493183","https://openalex.org/W3161227852"],"abstract_inverted_index":{"UDP-glucose":[0,7,108],"dehydrogenase":[1],"(UGDH)":[2],"catalyzes":[3],"two":[4],"oxidations":[5],"of":[6,14,24,36,42,114,142,183,202],"to":[8,21,110,135,165,223],"yield":[9],"UDP-glucuronic":[10,25],"acid.":[11],"Pathological":[12],"overproduction":[13],"extracellular":[15],"matrix":[16],"components":[17],"may":[18],"be":[19],"linked":[20],"the":[22,60,115,191,199,213,217,235],"availability":[23],"acid;":[26],"therefore":[27],"UGDH":[28,38,101],"is":[29,73,188,196,221],"an":[30],"intriguing":[31],"therapeutic":[32],"target.":[33],"Specific":[34],"inhibition":[35],"human":[37,61,71,218],"requires":[39],"detailed":[40],"knowledge":[41],"its":[43,65,210],"catalytic":[44,130,206],"mechanism,":[45],"which":[46],"has":[47],"not":[48,149],"been":[49],"characterized.":[50],"In":[51],"this":[52,121],"report,":[53],"we":[54,123],"have":[55,224],"cloned,":[56],"expressed,":[57],"and":[58,63,82,107,126,132,137,144,208,232],"affinity-purified":[59],"enzyme":[62,72,116],"determined":[64],"steady":[66],"state":[67],"kinetic":[68],"parameters.":[69],"The":[70],"active":[74,117,229],"as":[75,128,204],"a":[76,91,112,180,205,225],"hexamer":[77],"with":[78,87,104,198],"values":[79],"for":[80,90,98,190,216],"Km":[81],"Vmax":[83],"that":[84,162,186],"agree":[85],"well":[86],"those":[88],"reported":[89],"bovine":[92],"homolog.":[93],"We":[94],"used":[95],"crystal":[96],"coordinates":[97],"Streptococcus":[99],"pyogenes":[100],"in":[102,212,227,233],"complex":[103],"NAD+":[105],"cofactor":[106],"substrate":[109],"generate":[111],"model":[113],"site.":[118],"Based":[119],"on":[120],"model,":[122],"selected":[124],"Cys-276":[125,203],"Lys-279":[127,220],"likely":[129,222],"residues":[131,231],"converted":[133],"them":[134],"serine":[136],"alanine,":[138],"respectively.":[139],"Enzymatic":[140],"activity":[141],"C276S":[143],"K279A":[145,163],"point":[146],"mutants":[147],"was":[148],"measurable":[150],"under":[151],"normal":[152],"assay":[153],"conditions.":[154],"Rate":[155],"constants":[156],"measured":[157],"over":[158],"several":[159],"hours":[160],"demonstrated":[161],"continued":[164],"turn":[166],"over,":[167],"although":[168],"250-fold":[169],"more":[170],"slowly":[171],"than":[172],"wild":[173],"type":[174],"enzyme.":[175,219],"C276S,":[176],"however,":[177],"performed":[178],"only":[179],"single":[181],"round":[182],"oxidation,":[184],"indicating":[185],"it":[187],"essential":[189],"second":[192],"oxidation.":[193],"This":[194],"result":[195],"consistent":[197],"postulated":[200],"role":[201,226],"residue":[207],"supports":[209],"position":[211],"reaction":[214],"mechanism":[215],"positioning":[228],"site":[230],"maintaining":[234],"hexameric":[236],"quaternary":[237],"structure.":[238]},"counts_by_year":[{"year":2026,"cited_by_count":3},{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":5},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":1},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":5},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":2},{"year":2012,"cited_by_count":4}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2016-06-24T00:00:00"}