{"id":"https://openalex.org/W2129129082","doi":"https://doi.org/10.1128/jcm.43.7.3324-3333.2005","title":"Multilocus DNA Sequence Comparisons Rapidly Identify Pathogenic Molds","display_name":"Multilocus DNA Sequence Comparisons Rapidly Identify Pathogenic Molds","publication_year":2005,"publication_date":"2005-07-01","ids":{"openalex":"https://openalex.org/W2129129082","doi":"https://doi.org/10.1128/jcm.43.7.3324-3333.2005","mag":"2129129082","pmid":"https://pubmed.ncbi.nlm.nih.gov/16000456","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/1169180"},"language":"en","primary_location":{"id":"doi:10.1128/jcm.43.7.3324-3333.2005","doi":"https://doi.org/10.1128/jcm.43.7.3324-3333.2005","is_oa":false,"landing_page_url":"https://doi.org/10.1128/jcm.43.7.3324-3333.2005","pdf_url":null,"source":{"id":"https://openalex.org/S53087977","display_name":"Journal of Clinical Microbiology","issn_l":"0095-1137","issn":["0095-1137","1070-633X","1098-660X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Clinical Microbiology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1169180","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5040751195","display_name":"Jennifer L. Rakeman","orcid":"https://orcid.org/0000-0002-9464-7723","observed_orcids":["https://orcid.org/0000-0002-9464-7723"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Jennifer L. Rakeman","raw_affiliation_strings":["Departments of Laboratory Medicine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Laboratory Medicine","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5086972730","display_name":"Uyen Thi Tu Bui","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Uyen Bui","raw_affiliation_strings":["Departments of Laboratory Medicine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Laboratory Medicine","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5037965705","display_name":"Karen LaFe","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Karen LaFe","raw_affiliation_strings":["Departments of Laboratory Medicine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Laboratory Medicine","institution_ids":[]}]},{"author_position":"middle","author":{"id":null,"display_name":"Yi-Ching Chen","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Yi-Ching Chen","raw_affiliation_strings":["Departments of Laboratory Medicine"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Laboratory Medicine","institution_ids":[]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5026801488","display_name":"Rhonda J. Honeycutt","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Rhonda J. Honeycutt","raw_affiliation_strings":["Clarity BioSciences, Inc., Carlsbad, California"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Clarity BioSciences, Inc., Carlsbad, California","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5041519842","display_name":"Brad T. Cookson","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I201448701","display_name":"University of Washington","ror":"https://ror.org/00cvxb145","country_code":"US","type":"education","lineage":["https://openalex.org/I201448701"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Brad T. Cookson","raw_affiliation_strings":["Departments of Laboratory Medicine","Microbiology,University of Washington, Seattle, Washington"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Laboratory Medicine","institution_ids":[]},{"raw_affiliation_string":"Microbiology,University of Washington, Seattle, Washington","institution_ids":["https://openalex.org/I201448701"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5041519842"],"corresponding_institution_ids":["https://openalex.org/I201448701"],"apc_list":null,"apc_paid":null,"fwci":8.7663,"has_fulltext":false,"cited_by_count":128,"citation_normalized_percentile":{"value":0.97752548,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":99},"biblio":{"volume":"43","issue":"7","first_page":"3324","last_page":"3333"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10825","display_name":"Plant Pathogens and Fungal Diseases","score":0.6029999852180481,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10825","display_name":"Plant Pathogens and Fungal Diseases","score":0.6029999852180481,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10150","display_name":"Antifungal resistance and susceptibility","score":0.2029000073671341,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11037","display_name":"Fungal Infections and Studies","score":0.04390000179409981,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/fungal-identification","display_name":"fungal identification","score":0.7419999837875366},{"id":"https://openalex.org/keywords/its-sequences","display_name":"ITS sequences","score":0.6380000114440918},{"id":"https://openalex.org/keywords/dna-barcoding","display_name":"DNA barcoding","score":0.6150000095367432},{"id":"https://openalex.org/keywords/opportunistic-fungal-infection","display_name":"opportunistic fungal infection","score":0.8799999952316284},{"id":"https://openalex.org/keywords/clinical-isolates","display_name":"clinical isolates","score":0.6119999885559082},{"id":"https://openalex.org/keywords/reference-strains","display_name":"reference strains","score":0.5170000195503235},{"id":"https://openalex.org/keywords/fungal-phylogeny","display_name":"fungal phylogeny","score":0.7350000143051147},{"id":"https://openalex.org/keywords/sequevars","display_name":"sequevars","score":0.6380000114440918},{"id":"https://openalex.org/keywords/new-species-identification","display_name":"new species identification","score":0.3869999945163727}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6107895374298096},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.5370503664016724},{"id":"https://openalex.org/C51679486","wikidata":"https://www.wikidata.org/wiki/Q380546","display_name":"DNA sequencing","level":3,"score":0.5159045457839966},{"id":"https://openalex.org/C77434933","wikidata":"https://www.wikidata.org/wiki/Q4043440","display_name":"Multilocus sequence typing","level":4,"score":0.5042616128921509},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.5008530616760254},{"id":"https://openalex.org/C2778112365","wikidata":"https://www.wikidata.org/wiki/Q3511065","display_name":"Sequence (biology)","level":2,"score":0.49457189440727234},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.45575374364852905},{"id":"https://openalex.org/C135763542","wikidata":"https://www.wikidata.org/wiki/Q106016","display_name":"Genotype","level":3,"score":0.21881768107414246},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.17709386348724365}],"mesh":[{"descriptor_ui":"D004271","descriptor_name":"DNA, Fungal","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D004271","descriptor_name":"DNA, Fungal","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D005658","descriptor_name":"Fungi","qualifier_ui":"Q000145","qualifier_name":"classification","is_major_topic":true},{"descriptor_ui":"D005658","descriptor_name":"Fungi","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D005658","descriptor_name":"Fungi","qualifier_ui":"Q000472","qualifier_name":"pathogenicity","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009181","descriptor_name":"Mycoses","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":true},{"descriptor_ui":"D010641","descriptor_name":"Phenotype","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010802","descriptor_name":"Phylogeny","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011110","descriptor_name":"Polymorphism, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D012339","descriptor_name":"RNA, Ribosomal, 28S","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D013997","descriptor_name":"Time Factors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016533","descriptor_name":"Mycological Typing Techniques","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D017422","descriptor_name":"Sequence Analysis, DNA","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D021903","descriptor_name":"DNA, Ribosomal Spacer","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1128/jcm.43.7.3324-3333.2005","doi":"https://doi.org/10.1128/jcm.43.7.3324-3333.2005","is_oa":false,"landing_page_url":"https://doi.org/10.1128/jcm.43.7.3324-3333.2005","pdf_url":null,"source":{"id":"https://openalex.org/S53087977","display_name":"Journal of Clinical Microbiology","issn_l":"0095-1137","issn":["0095-1137","1070-633X","1098-660X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Clinical Microbiology","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:1801714","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1169180","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmid:16000456","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/16000456","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of clinical microbiology","raw_type":"Comparative Study"},{"id":"pmh:oai:pubmedcentral.nih.gov:1169180","doi":null,"is_oa":true,"landing_page_url":"https://pmc.ncbi.nlm.nih.gov/articles/PMC1169180/","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Clin Microbiol","raw_type":"Text"}],"best_oa_location":{"id":"pmh:oai:europepmc.org:1801714","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1169180","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.4043000042438507,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1637171650","display_name":"STRATEGIES TO IDENTIFY AND DISTINGUISH FUNGAL PATHOGENS","funder_award_id":"R44GM057669","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G5867594902","display_name":null,"funder_award_id":"7R44GM57669-04","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320310094","display_name":"University of Washington","ror":"https://ror.org/00cvxb145"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":56,"referenced_works":["https://openalex.org/W4154944","https://openalex.org/W369694008","https://openalex.org/W574432219","https://openalex.org/W593080422","https://openalex.org/W1457665616","https://openalex.org/W1577160752","https://openalex.org/W1595052334","https://openalex.org/W1923958806","https://openalex.org/W1987429715","https://openalex.org/W1990617776","https://openalex.org/W1996044602","https://openalex.org/W1997746489","https://openalex.org/W2003468457","https://openalex.org/W2006193342","https://openalex.org/W2010458649","https://openalex.org/W2014188009","https://openalex.org/W2014427579","https://openalex.org/W2019423755","https://openalex.org/W2045138757","https://openalex.org/W2062447170","https://openalex.org/W2066833884","https://openalex.org/W2069273541","https://openalex.org/W2092912913","https://openalex.org/W2096720044","https://openalex.org/W2097382368","https://openalex.org/W2097720062","https://openalex.org/W2098175638","https://openalex.org/W2099881697","https://openalex.org/W2110182011","https://openalex.org/W2110702182","https://openalex.org/W2111628103","https://openalex.org/W2121347108","https://openalex.org/W2123412399","https://openalex.org/W2131628610","https://openalex.org/W2135053160","https://openalex.org/W2136771159","https://openalex.org/W2137266954","https://openalex.org/W2143639041","https://openalex.org/W2146237316","https://openalex.org/W2148003631","https://openalex.org/W2150297520","https://openalex.org/W2150635951","https://openalex.org/W2153561015","https://openalex.org/W2153948746","https://openalex.org/W2157077695","https://openalex.org/W2162747969","https://openalex.org/W2165835017","https://openalex.org/W2166785990","https://openalex.org/W2166787752","https://openalex.org/W2168633740","https://openalex.org/W2171111838","https://openalex.org/W2979018886","https://openalex.org/W2989345970","https://openalex.org/W3021230948","https://openalex.org/W3183559403","https://openalex.org/W4285719527"],"related_works":["https://openalex.org/W2061568194","https://openalex.org/W2937632628","https://openalex.org/W2269093315","https://openalex.org/W2639406310","https://openalex.org/W3088371252","https://openalex.org/W176166660","https://openalex.org/W2099892012","https://openalex.org/W2143639041","https://openalex.org/W2106923956","https://openalex.org/W1923958806"],"abstract_inverted_index":{"The":[0],"increasing":[1],"incidence":[2],"of":[3,12,35,55,71,76,91,126,143,146,156,162,233,243],"opportunistic":[4],"fungal":[5],"infections":[6],"necessitates":[7],"rapid":[8],"and":[9,33,81,89,103,106,113,136,149,174,189,219],"accurate":[10],"identification":[11,23,39,155],"the":[13,31,69,72,77,82,92],"associated":[14],"fungi":[15],"to":[16,207,223],"facilitate":[17],"optimal":[18],"patient":[19],"treatment.":[20],"Traditional":[21],"phenotype-based":[22],"methods":[24,192],"utilized":[25,222],"in":[26,179,205],"clinical":[27,101],"laboratories":[28],"rely":[29],"on":[30,199],"production":[32],"recognition":[34],"reproductive":[36],"structures,":[37],"making":[38],"difficult":[40],"or":[41],"impossible":[42],"when":[43],"these":[44],"structures":[45],"are":[46,203],"not":[47],"observed.":[48],"We":[49,120],"hypothesized":[50],"that":[51,212],"DNA":[52,130,151],"sequence":[53,152,170,201],"analysis":[54,70,153,202],"multiple":[56],"loci":[57],"is":[58],"useful":[59,238],"for":[60,239],"rapidly":[61],"identifying":[62,240],"medically":[63],"important":[64,226],"molds.":[65,227,245],"Our":[66],"study":[67],"included":[68],"D1/D2":[73],"hypervariable":[74],"region":[75],"28S":[78],"ribosomal":[79],"gene":[80],"internal":[83],"transcribed":[84],"spacer":[85],"(ITS)":[86],"regions":[87],"1":[88],"2":[90],"rRNA":[93],"operon.":[94],"Two":[95],"hundred":[96],"one":[97,114],"strains,":[98,108],"including":[99],"143":[100],"isolates":[102],"58":[104],"reference":[105],"type":[107],"representing":[109],"43":[110],"recognized":[111],"species":[112,145,158,164,242],"possible":[115],"new":[116,241],"species,":[117,185],"were":[118,177,193],"examined.":[119],"generated":[121],"a":[122],"phenotypically":[123,230],"validated":[124,231],"database":[125,232],"118":[127],"diagnostic":[128],"alleles.":[129],"length":[131],"polymorphisms":[132],"detected":[133,178],"among":[134],"ITS1":[135,173],"ITS2":[137],"PCR":[138],"products":[139],"can":[140,220],"differentiate":[141],"20":[142],"33":[144],"molds":[147],"tested,":[148,165],"ITS":[150,200,213,234],"permits":[154],"all":[157,183],"tested.":[159],"For":[160,182],"42":[161],"44":[163,184],"conspecific":[166],"strains":[167],"displayed":[168],">99%":[169],"identity":[171],"at":[172],"ITS2;":[175],"sequevars":[176],"two":[180],"species.":[181],"identifications":[186],"by":[187],"genotypic":[188],"traditional":[190],"phenotypic":[191],"100%":[194],"concordant.":[195],"Because":[196],"dendrograms":[197],"based":[198],"similar":[204],"topology":[206],"28S-based":[208],"trees,":[209],"we":[210],"conclude":[211],"sequences":[214,235],"provide":[215],"phylogenetically":[216],"valid":[217],"information":[218],"be":[221,237],"identify":[224],"clinically":[225],"Additionally,":[228],"this":[229],"will":[236],"pathogenic":[244]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":4},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":3},{"year":2022,"cited_by_count":6},{"year":2021,"cited_by_count":7},{"year":2020,"cited_by_count":6},{"year":2019,"cited_by_count":5},{"year":2018,"cited_by_count":5},{"year":2017,"cited_by_count":6},{"year":2016,"cited_by_count":6},{"year":2015,"cited_by_count":4},{"year":2014,"cited_by_count":9},{"year":2013,"cited_by_count":6},{"year":2012,"cited_by_count":8}],"updated_date":"2026-11-10T11:39:43.021295","created_date":"2016-06-24T00:00:00"}