{"id":"https://openalex.org/W2128806579","doi":"https://doi.org/10.1099/00221287-97-2-257","title":"Growth on D-Lyxose of a Mutant Strain of Escherichia coli K12 Using a Novel Isomerase and Enzymes Related to D-Xylose Metabolism","display_name":"Growth on D-Lyxose of a Mutant Strain of Escherichia coli K12 Using a Novel Isomerase and Enzymes Related to D-Xylose Metabolism","publication_year":1976,"publication_date":"1976-12-01","ids":{"openalex":"https://openalex.org/W2128806579","doi":"https://doi.org/10.1099/00221287-97-2-257","mag":"2128806579","pmid":"https://pubmed.ncbi.nlm.nih.gov/796410"},"language":"en","primary_location":{"id":"doi:10.1099/00221287-97-2-257","doi":"https://doi.org/10.1099/00221287-97-2-257","is_oa":false,"landing_page_url":"https://doi.org/10.1099/00221287-97-2-257","pdf_url":null,"source":{"id":"https://openalex.org/S4210238889","display_name":"Journal of General Microbiology","issn_l":"0022-1287","issn":["0022-1287","2059-9323"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310320497","host_organization_name":"Microbiology Society","host_organization_lineage":["https://openalex.org/P4310320497"],"host_organization_lineage_names":["Microbiology Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of General Microbiology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5108232641","display_name":"Fred J. Stevens","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I111979921","display_name":"Northwestern University","ror":"https://ror.org/000e0be47","country_code":"US","type":"education","lineage":["https://openalex.org/I111979921"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"F. J. STEVENS","raw_affiliation_strings":["Departments of Biochemistry and Molecular Biology, and Engineering Sciences and Applied Mathematics, Northwestern University, Evanston, Illinois 60201, U.S.A","Northwestern University****"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Biochemistry and Molecular Biology, and Engineering Sciences and Applied Mathematics, Northwestern University, Evanston, Illinois 60201, U.S.A","institution_ids":["https://openalex.org/I111979921"]},{"raw_affiliation_string":"Northwestern University****","institution_ids":["https://openalex.org/I111979921"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5001064458","display_name":"T T Wu","orcid":"https://orcid.org/0009-0001-4824-8844","observed_orcids":["https://orcid.org/0009-0001-4824-8844"]},"institutions":[{"id":"https://openalex.org/I111979921","display_name":"Northwestern University","ror":"https://ror.org/000e0be47","country_code":"US","type":"education","lineage":["https://openalex.org/I111979921"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"T. T. WU","raw_affiliation_strings":["Departments of Biochemistry and Molecular Biology, and Engineering Sciences and Applied Mathematics, Northwestern University, Evanston, Illinois 60201, U.S.A","Molecular Biosciences"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Departments of Biochemistry and Molecular Biology, and Engineering Sciences and Applied Mathematics, Northwestern University, Evanston, Illinois 60201, U.S.A","institution_ids":["https://openalex.org/I111979921"]},{"raw_affiliation_string":"Molecular Biosciences","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I111979921"],"apc_list":null,"apc_paid":null,"fwci":0.6203,"has_fulltext":false,"cited_by_count":22,"citation_normalized_percentile":{"value":0.69517458,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":96},"biblio":{"volume":"97","issue":"2","first_page":"257","last_page":"265"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.961899995803833,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.961899995803833,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12688","display_name":"Microbial metabolism and enzyme function","score":0.01360000018030405,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.007799999788403511,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/escherichia-coli-k-12","display_name":"Escherichia coli K-12","score":0.9129999876022339},{"id":"https://openalex.org/keywords/d-xylulose","display_name":"D-xylulose","score":0.7720000147819519},{"id":"https://openalex.org/keywords/mutant-strain","display_name":"mutant strain","score":0.5139999985694885},{"id":"https://openalex.org/keywords/carbon-source-utilization","display_name":"carbon source utilization","score":0.5210000276565552},{"id":"https://openalex.org/keywords/sugar-transport","display_name":"sugar transport","score":0.35600000619888306}],"concepts":[{"id":"https://openalex.org/C2781264208","wikidata":"https://www.wikidata.org/wiki/Q118026","display_name":"Isomerase","level":3,"score":0.7998263239860535},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.7079805135726929},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.7070656418800354},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.6948551535606384},{"id":"https://openalex.org/C190114821","wikidata":"https://www.wikidata.org/wiki/Q412861","display_name":"Permease","level":4,"score":0.6846604943275452},{"id":"https://openalex.org/C2780294150","wikidata":"https://www.wikidata.org/wiki/Q66589603","display_name":"Xylose","level":3,"score":0.6794992685317993},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.6265089511871338},{"id":"https://openalex.org/C2779277513","wikidata":"https://www.wikidata.org/wiki/Q8045529","display_name":"Xylose isomerase","level":4,"score":0.5227494239807129},{"id":"https://openalex.org/C2778022156","wikidata":"https://www.wikidata.org/wiki/Q576145","display_name":"Strain (injury)","level":2,"score":0.5197567343711853},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.46849140524864197},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4634743630886078},{"id":"https://openalex.org/C5527404","wikidata":"https://www.wikidata.org/wiki/Q2357310","display_name":"Structural gene","level":4,"score":0.4400979280471802},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4047987759113312},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.3247467279434204},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.1518705189228058},{"id":"https://openalex.org/C100544194","wikidata":"https://www.wikidata.org/wiki/Q41760","display_name":"Fermentation","level":2,"score":0.05065229535102844},{"id":"https://openalex.org/C105702510","wikidata":"https://www.wikidata.org/wiki/Q514","display_name":"Anatomy","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D002238","descriptor_name":"Carbohydrate Epimerases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D005632","descriptor_name":"Fructose","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005796","descriptor_name":"Genes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008358","descriptor_name":"Mannose","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D010429","descriptor_name":"Pentoses","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D013237","descriptor_name":"Stereoisomerism","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014994","descriptor_name":"Xylose","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014996","descriptor_name":"Xylulose","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1099/00221287-97-2-257","doi":"https://doi.org/10.1099/00221287-97-2-257","is_oa":false,"landing_page_url":"https://doi.org/10.1099/00221287-97-2-257","pdf_url":null,"source":{"id":"https://openalex.org/S4210238889","display_name":"Journal of General Microbiology","issn_l":"0022-1287","issn":["0022-1287","2059-9323"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310320497","host_organization_name":"Microbiology Society","host_organization_lineage":["https://openalex.org/P4310320497"],"host_organization_lineage_names":["Microbiology Society"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of General Microbiology","raw_type":"journal-article"},{"id":"pmid:796410","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/796410","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of general microbiology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":10,"referenced_works":["https://openalex.org/W185465889","https://openalex.org/W1501168178","https://openalex.org/W1622205958","https://openalex.org/W1663588906","https://openalex.org/W1775749144","https://openalex.org/W1812531649","https://openalex.org/W1981347019","https://openalex.org/W2096211502","https://openalex.org/W2163355325","https://openalex.org/W2615750665"],"related_works":["https://openalex.org/W2123718084","https://openalex.org/W2468145109","https://openalex.org/W2133846511","https://openalex.org/W4248437720","https://openalex.org/W1564420089","https://openalex.org/W2110353339","https://openalex.org/W2088521375","https://openalex.org/W2001733041","https://openalex.org/W1553279638","https://openalex.org/W2076304780"],"abstract_inverted_index":{"Escherichia":[0],"coli":[1,82],"K12":[2],"cannot":[3],"grow":[4],"on":[5,79],"D-lyxose,":[6],"but":[7],"a":[8,39],"mutant":[9,36],"was":[10,106],"isolated":[11],"which":[12,42],"can":[13],"utilize":[14],"D-lyxose":[15,67],"as":[16],"sole":[17],"source":[18],"of":[19,52,98,111],"carbon":[20],"and":[21,109],"energy":[22],"for":[23],"growth.":[24],"D-Lyxose":[25],"is":[26,43,73,92],"transported":[27],"into":[28,68],"the":[29,32,47,53,80,96,99],"bacteria":[30],"by":[31,95],"D-xylose":[33,101],"permease.":[34],"The":[35],"constitutively":[37],"synthesizes":[38],"new":[40],"isomerase":[41,89,105],"not":[44],"inducible":[45],"in":[46],"parent":[48],"strain":[49],"under":[50],"any":[51],"conditions":[54],"tested.":[55],"This":[56],"enzyme,":[57],"whose":[58],"native":[59],"substrate":[60],"appears":[61],"to":[62],"be":[63],"D-mannose,":[64],"fortuitously":[65],"converts":[66],"D-xylulose.":[69],"Its":[70],"structural":[71],"gene":[72],"located":[74],"at":[75],"around":[76],"85":[77],"min":[78],"E.":[81],"genetic":[83],"map,":[84],"away":[85],"from":[86],"other":[87],"known":[88],"genes.":[90],"D-Xylulose":[91],"subsequently":[93],"catabolized":[94],"enzymes":[97],"normal":[100],"metabolic":[102],"pathway.":[103],"D-Mannose":[104],"partially":[107],"purified":[108],"some":[110],"its":[112],"properties":[113],"were":[114],"examined.":[115]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}