{"id":"https://openalex.org/W21286099","doi":null,"title":"Artificial Cellulosomes and Arsenic Cleanup: From Single Cell Programming to Synthetic Yeast Consortium","display_name":"Artificial Cellulosomes and Arsenic Cleanup: From Single Cell Programming to Synthetic Yeast Consortium","publication_year":2011,"publication_date":"2011-01-01","ids":{"openalex":"https://openalex.org/W21286099","mag":"21286099"},"language":"en","primary_location":{"id":"pmh:oai:escholarship.org/ark:/13030/qt0fb629h4","doi":null,"is_oa":false,"landing_page_url":"https://escholarship.org/uc/item/0fb629h4","pdf_url":null,"source":{"id":"https://openalex.org/S4306400115","display_name":"eScholarship (California Digital Library)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2801248553","host_organization_name":"California Digital Library","host_organization_lineage":["https://openalex.org/I2801248553"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"etd"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5071900587","display_name":"Shen\u2010Long Tsai","orcid":"https://orcid.org/0000-0002-1387-0828","observed_orcids":["https://orcid.org/0000-0002-1387-0828"]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Shen\u2010Long Tsai","raw_affiliation_strings":[],"raw_orcid":"https://orcid.org/0000-0002-1387-0828","affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5071900587"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10171","display_name":"Biofuel production and bioconversion","score":0.9369999766349792,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T10171","display_name":"Biofuel production and bioconversion","score":0.9369999766349792,"subfield":{"id":"https://openalex.org/subfields/2204","display_name":"Biomedical Engineering"},"field":{"id":"https://openalex.org/fields/22","display_name":"Engineering"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.042399998754262924,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10497","display_name":"Fungal and yeast genetics research","score":0.00800000037997961,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cellulosome","display_name":"cellulosome","score":0.8389999866485596},{"id":"https://openalex.org/keywords/cell-surface-display","display_name":"cell surface display","score":0.6140000224113464},{"id":"https://openalex.org/keywords/saccharomyces-cerevisiae","display_name":"Saccharomyces cerevisiae","score":0.9070000052452087},{"id":"https://openalex.org/keywords/second-generation-bioethanol","display_name":"cellulosic ethanol","score":0.7409999966621399},{"id":"https://openalex.org/keywords/consolidated-bioprocessing","display_name":"consolidated bioprocessing","score":0.9380000233650208},{"id":"https://openalex.org/keywords/simultaneous-saccharification-and-fermentation","display_name":"simultaneous saccharification and fermentation","score":0.7850000262260437},{"id":"https://openalex.org/keywords/cellulolytic-enzymes","display_name":"cellulolytic enzymes","score":0.7730000019073486},{"id":"https://openalex.org/keywords/enzyme-synergy","display_name":"enzyme synergy","score":0.640999972820282},{"id":"https://openalex.org/keywords/cysteine-desulfhydrase","display_name":"cysteine desulfhydrase","score":0.9760000109672546},{"id":"https://openalex.org/keywords/metabolic-engineering","display_name":"metabolic engineering","score":0.6690000295639038},{"id":"https://openalex.org/keywords/bioremediation","display_name":"bioremediation","score":0.7710000276565552},{"id":"https://openalex.org/keywords/biofuel-production","display_name":"biofuel production","score":0.8370000123977661}],"concepts":[{"id":"https://openalex.org/C163283067","wikidata":"https://www.wikidata.org/wiki/Q1053121","display_name":"Cellulosic ethanol","level":3,"score":0.8243790864944458},{"id":"https://openalex.org/C4916135","wikidata":"https://www.wikidata.org/wiki/Q4119055","display_name":"Bioprocess","level":2,"score":0.712095320224762},{"id":"https://openalex.org/C53991642","wikidata":"https://www.wikidata.org/wiki/Q128991","display_name":"Biofuel","level":2,"score":0.6727009415626526},{"id":"https://openalex.org/C141603559","wikidata":"https://www.wikidata.org/wiki/Q410324","display_name":"Cellulase","level":3,"score":0.6068971157073975},{"id":"https://openalex.org/C2776029434","wikidata":"https://www.wikidata.org/wiki/Q5058394","display_name":"Cellulosome","level":5,"score":0.6031379103660583},{"id":"https://openalex.org/C6350086","wikidata":"https://www.wikidata.org/wiki/Q6822347","display_name":"Metabolic engineering","level":3,"score":0.5414405465126038},{"id":"https://openalex.org/C2779251873","wikidata":"https://www.wikidata.org/wiki/Q80294","display_name":"Cellulose","level":2,"score":0.5290870666503906},{"id":"https://openalex.org/C65183824","wikidata":"https://www.wikidata.org/wiki/Q194361","display_name":"Ethanol fuel","level":3,"score":0.5153469443321228},{"id":"https://openalex.org/C150903083","wikidata":"https://www.wikidata.org/wiki/Q7108","display_name":"Biotechnology","level":1,"score":0.4810045659542084},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.4778563380241394},{"id":"https://openalex.org/C183696295","wikidata":"https://www.wikidata.org/wiki/Q2487696","display_name":"Biochemical engineering","level":1,"score":0.4572122097015381},{"id":"https://openalex.org/C2779222958","wikidata":"https://www.wikidata.org/wiki/Q45422","display_name":"Yeast","level":2,"score":0.4337380826473236},{"id":"https://openalex.org/C53270655","wikidata":"https://www.wikidata.org/wiki/Q5381536","display_name":"Enzymatic hydrolysis","level":3,"score":0.410228967666626},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.30128276348114014},{"id":"https://openalex.org/C94412978","wikidata":"https://www.wikidata.org/wiki/Q103135","display_name":"Hydrolysis","level":2,"score":0.2787228226661682},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.17866715788841248},{"id":"https://openalex.org/C127413603","wikidata":"https://www.wikidata.org/wiki/Q11023","display_name":"Engineering","level":0,"score":0.1513039767742157},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.09122207760810852},{"id":"https://openalex.org/C42360764","wikidata":"https://www.wikidata.org/wiki/Q83588","display_name":"Chemical engineering","level":1,"score":0.08359909057617188},{"id":"https://openalex.org/C2776061948","wikidata":"https://www.wikidata.org/wiki/Q3681172","display_name":"Clostridium thermocellum","level":4,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:escholarship.org/ark:/13030/qt0fb629h4","doi":null,"is_oa":false,"landing_page_url":"https://escholarship.org/uc/item/0fb629h4","pdf_url":null,"source":{"id":"https://openalex.org/S4306400115","display_name":"eScholarship (California Digital Library)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2801248553","host_organization_name":"California Digital Library","host_organization_lineage":["https://openalex.org/I2801248553"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"etd"},{"id":"mag:21286099","doi":null,"is_oa":false,"landing_page_url":"https://escholarship.org/content/qt0fb629h4/qt0fb629h4.pdf?t=mi3qst","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Responsible consumption and production","id":"https://metadata.un.org/sdg/12","score":0.807699978351593},{"display_name":"Affordable and clean energy","id":"https://metadata.un.org/sdg/7","score":0.75}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":43,"referenced_works":["https://openalex.org/W1518129554","https://openalex.org/W1524653649","https://openalex.org/W1533167872","https://openalex.org/W1896918741","https://openalex.org/W1924734434","https://openalex.org/W1966642263","https://openalex.org/W1967390330","https://openalex.org/W1967839793","https://openalex.org/W1968834637","https://openalex.org/W1973782344","https://openalex.org/W1974858047","https://openalex.org/W1979185535","https://openalex.org/W1991694218","https://openalex.org/W1995574044","https://openalex.org/W2002156802","https://openalex.org/W2005291574","https://openalex.org/W2018731408","https://openalex.org/W2030815168","https://openalex.org/W2037355346","https://openalex.org/W2039755166","https://openalex.org/W2041711159","https://openalex.org/W2042752032","https://openalex.org/W2048080349","https://openalex.org/W2053038740","https://openalex.org/W2053658205","https://openalex.org/W2058867396","https://openalex.org/W2078833660","https://openalex.org/W2086538139","https://openalex.org/W2090351763","https://openalex.org/W2096818569","https://openalex.org/W2102957972","https://openalex.org/W2103336541","https://openalex.org/W2110225754","https://openalex.org/W2121108655","https://openalex.org/W2125312027","https://openalex.org/W2135038396","https://openalex.org/W2141905730","https://openalex.org/W2142686222","https://openalex.org/W2149602337","https://openalex.org/W2150272062","https://openalex.org/W2156911822","https://openalex.org/W2159595851","https://openalex.org/W2165883761"],"related_works":["https://openalex.org/W2286918735","https://openalex.org/W1998441255","https://openalex.org/W1909198216","https://openalex.org/W2158855548","https://openalex.org/W2146906453","https://openalex.org/W2156911822","https://openalex.org/W2079260562","https://openalex.org/W2362337964","https://openalex.org/W2120081940","https://openalex.org/W3149887618"],"abstract_inverted_index":{"As":[0],"our":[1],"society":[2],"marches":[3],"toward":[4,320],"a":[5,41,197,208,214,218,226,275],"more":[6,219],"technologically-inclined":[7],"and":[8,12,49,57,65,85,99,110,140,143,259],"industrialized":[9],"future,":[10],"energy":[11],"environmental":[13,321],"sustainability":[14],"are":[15],"two":[16],"of":[17,28,43,79,91,124,145,151,230,271,283,293,337],"the":[18,26,77,132,164,169,190,234,246,266,272,280,284,291,316],"most":[19],"challenging":[20],"problems":[21],"we":[22],"face":[23],"today.":[24],"With":[25],"aid":[27],"recent":[29],"advances":[30],"in":[31,103,196,250,301],"recombinant":[32],"molecular":[33],"technology,":[34],"metabolic":[35,80,317],"engineering":[36,81,318],"has":[37,53,58],"been":[38],"employed":[39],"on":[40,76,131,155,233,252,279],"variety":[42],"host":[44],"organisms":[45],"to":[46,70,95,106,147,204,289,310],"improve":[47],"biosorption":[48],"biocatalytic":[50],"capabilities.":[51],"This":[52],"shown":[54],"immense":[55],"promise":[56],"become":[59],"an":[60,97,104,340],"attractive":[61],"tool":[62],"for":[63,82,138,181,192,217,255,334,343],"bioremediation":[64],"biofuel":[66,83],"production.":[67],"In":[68,243],"regards":[69],"these":[71],"challenges,":[72],"this":[73,92,244],"dissertation":[74,93],"focuses":[75],"use":[78],"production":[84,200,258,270,303],"arsenic":[86,338],"remediation.":[87],"The":[88,117,149,186,298],"first":[89,160],"objective":[90],"was":[94,120,159,240,296,308],"create":[96,213],"efficient":[98,341],"inexpensive":[100],"whole-cell":[101],"biocatalyst":[102],"effort":[105],"produce":[107],"economically":[108],"compatible":[109],"sustainable":[111],"biofuels,":[112],"such":[113],"as":[114,339],"cellulosic":[115],"ethanol.":[116,148],"approach":[118],"used":[119],"via":[121,237],"surface":[122,236],"display":[123],"versatile":[125],"cellulolytic":[126,176],"enzyme":[127,306],"complexes,":[128],"namely":[129],"cellulosomes,":[130],"historical":[133],"ethanol":[134,199,257,269,302],"producer":[135],"Saccharomyces":[136],"cerevisiae":[137,325],"simultaneous":[139],"synergistic":[141],"saccharification":[142],"fermentation":[144],"cellulose":[146,183,193],"feasibility":[150],"assembling":[152],"cellulosome":[153],"structures":[154],"yeast":[156,253,273],"cell":[157,172,235,260],"surfaces":[158,254],"demonstrated":[161],"by":[162],"incubating":[163],"miniscaffoldin":[165],"displayed":[166],"yeasts":[167],"with":[168],"Escherichia":[170],"coli":[171],"lysates":[173],"containing":[174],"three":[175],"enzymes":[177],"that":[178,205,305],"were":[179,248,332],"necessary":[180],"hydrolyzing":[182],"into":[184],"glucose.":[185],"functionally-assembled":[187],"minicelluosomes":[188],"retained":[189],"synergism":[191],"hydrolysis,":[194],"resulting":[195],"higher":[198],"level":[201],"when":[202],"compared":[203],"obtained":[206],"from":[207,262],"free":[209],"cellulase":[210],"system.":[211],"To":[212,264,313],"microorganism":[215],"suitable":[216],"cost-effective":[220],"process,":[221],"called":[222],"consolidated":[223],"bioprocessing":[224],"(CBP),":[225],"synthetic":[227],"consortium":[228],"capable":[229],"displaying":[231],"mini-cellulosomes":[232],"intercellular":[238],"complementation":[239],"subsequently":[241],"created.":[242,297],"case,":[245],"minicellulosomes":[247],"assembled":[249],"vivo":[251],"direct":[256],"growth":[261],"cellulose.":[263],"tackle":[265],"relatively":[267],"modest":[268],"consortium,":[274],"designer":[276],"cellulosme":[277],"based":[278],"unique":[281],"feature":[282],"anchoring":[285],"-adaptor":[286],"scaffoldin":[287],"strategy":[288,319],"amplify":[290],"number":[292],"enzymatic":[294],"subunits":[295],"increased":[299],"rate":[300],"indicated":[304],"proximity":[307],"crucial":[309],"cellulosomal":[311],"synergy.":[312],"further":[314],"extend":[315],"sustainability,":[322],"engineered":[323],"S.":[324],"strains":[326],"expressing":[327],"cysteine":[328],"desulfhydrase":[329],"and/or":[330],"AtPCS":[331],"created":[333],"enhanced":[335],"accumulation":[336],"biosorbent":[342],"environment":[344],"cleanup.":[345]},"counts_by_year":[],"updated_date":"2026-08-18T07:49:30.821534","created_date":"2016-06-24T00:00:00"}
