{"id":"https://openalex.org/W2127394656","doi":"https://doi.org/10.1074/jbc.m414471200","title":"Structural Rationale for the Affinity of Pico- and Femtomolar Transition State Analogues of Escherichia coli 5\u2032-Methylthioadenosine/S-Adenosylhomocysteine Nucleosidase","display_name":"Structural Rationale for the Affinity of Pico- and Femtomolar Transition State Analogues of Escherichia coli 5\u2032-Methylthioadenosine/S-Adenosylhomocysteine Nucleosidase","publication_year":2005,"publication_date":"2005-03-04","ids":{"openalex":"https://openalex.org/W2127394656","doi":"https://doi.org/10.1074/jbc.m414471200","mag":"2127394656","pmid":"https://pubmed.ncbi.nlm.nih.gov/15746096"},"language":"en","primary_location":{"id":"doi:10.1074/jbc.m414471200","doi":"https://doi.org/10.1074/jbc.m414471200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m414471200","pdf_url":"http://www.jbc.org/article/S0021925820657763/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"http://www.jbc.org/article/S0021925820657763/pdf","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5102846930","display_name":"Jeffrey E. Lee","orcid":"https://orcid.org/0000-0002-2172-6586","observed_orcids":["https://orcid.org/0000-0002-2172-6586"]},"institutions":[{"id":"https://openalex.org/I185261750","display_name":"University of Toronto","ror":"https://ror.org/03dbr7087","country_code":"CA","type":"education","lineage":["https://openalex.org/I185261750"]},{"id":"https://openalex.org/I2801317318","display_name":"Hospital for Sick Children","ror":"https://ror.org/057q4rt57","country_code":"CA","type":"healthcare","lineage":["https://openalex.org/I2801317318"]}],"countries":["CA"],"is_corresponding":true,"raw_author_name":"Jeffrey E. Lee","raw_affiliation_strings":["Structural Biology and Biochemistry, Research Institute, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada","Department of Biochemistry, Faculty of Medicine, University of Toronto, Medical Sciences Building, Toronto, Ontario M5S 1A8, Canada, the","Structural Biology and Biochemistry, Research Institute, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada, the"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Structural Biology and Biochemistry, Research Institute, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada","institution_ids":["https://openalex.org/I2801317318"]},{"raw_affiliation_string":"Department of Biochemistry, Faculty of Medicine, University of Toronto, Medical Sciences Building, Toronto, Ontario M5S 1A8, Canada, the","institution_ids":["https://openalex.org/I185261750"]},{"raw_affiliation_string":"Structural Biology and Biochemistry, Research Institute, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada, the","institution_ids":["https://openalex.org/I2801317318"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109142072","display_name":"Vipender Singh","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I129975664","display_name":"Albert Einstein College of Medicine","ror":"https://ror.org/05cf8a891","country_code":"US","type":"education","lineage":["https://openalex.org/I129975664","https://openalex.org/I4210112371"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Vipender Singh","raw_affiliation_strings":["Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461, the"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461, the","institution_ids":["https://openalex.org/I129975664"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5067610098","display_name":"Gary B. Evans","orcid":"https://orcid.org/0000-0002-6973-2002","observed_orcids":["https://orcid.org/0000-0002-6973-2002"]},"institutions":[{"id":"https://openalex.org/I4387155704","display_name":"Industrial Research Limited","ror":"https://ror.org/011bwkg92","country_code":"NZ","type":"government","lineage":["https://openalex.org/I150510651","https://openalex.org/I4210126329","https://openalex.org/I4387155704"]}],"countries":["NZ"],"is_corresponding":false,"raw_author_name":"Gary B. Evans","raw_affiliation_strings":["Carbohydrate Chemistry Team, Industrial Research Limited, P. O. Box 31-310, Lower Hutt, New Zealand, the"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Carbohydrate Chemistry Team, Industrial Research Limited, P. O. Box 31-310, Lower Hutt, New Zealand, the","institution_ids":["https://openalex.org/I4387155704"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5015994250","display_name":"Peter C. Tyler","orcid":"https://orcid.org/0000-0002-3151-6208","observed_orcids":["https://orcid.org/0000-0002-3151-6208"]},"institutions":[{"id":"https://openalex.org/I4387155704","display_name":"Industrial Research Limited","ror":"https://ror.org/011bwkg92","country_code":"NZ","type":"government","lineage":["https://openalex.org/I150510651","https://openalex.org/I4210126329","https://openalex.org/I4387155704"]}],"countries":["NZ"],"is_corresponding":false,"raw_author_name":"Peter C. Tyler","raw_affiliation_strings":["Carbohydrate Chemistry Team, Industrial Research Limited, P. O. Box 31-310, Lower Hutt, New Zealand, the"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Carbohydrate Chemistry Team, Industrial Research Limited, P. O. Box 31-310, Lower Hutt, New Zealand, the","institution_ids":["https://openalex.org/I4387155704"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5060739053","display_name":"Richard H. Furneaux","orcid":"https://orcid.org/0000-0003-0973-2606","observed_orcids":["https://orcid.org/0000-0003-0973-2606"]},"institutions":[{"id":"https://openalex.org/I4387155704","display_name":"Industrial Research Limited","ror":"https://ror.org/011bwkg92","country_code":"NZ","type":"government","lineage":["https://openalex.org/I150510651","https://openalex.org/I4210126329","https://openalex.org/I4387155704"]}],"countries":["NZ"],"is_corresponding":false,"raw_author_name":"Richard H. Furneaux","raw_affiliation_strings":["Carbohydrate Chemistry Team, Industrial Research Limited, P. O. Box 31-310, Lower Hutt, New Zealand, the"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Carbohydrate Chemistry Team, Industrial Research Limited, P. O. Box 31-310, Lower Hutt, New Zealand, the","institution_ids":["https://openalex.org/I4387155704"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5088904728","display_name":"Ken Cornell","orcid":"https://orcid.org/0000-0003-3959-2499","observed_orcids":["https://orcid.org/0000-0003-3959-2499"]},"institutions":[{"id":"https://openalex.org/I120156002","display_name":"Boise State University","ror":"https://ror.org/02e3zdp86","country_code":"US","type":"education","lineage":["https://openalex.org/I120156002"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kenneth A. Cornell","raw_affiliation_strings":["Department of Chemistry, Boise State University, Boise, Idaho 83725-1520, the"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Boise State University, Boise, Idaho 83725-1520, the","institution_ids":["https://openalex.org/I120156002"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5012463920","display_name":"Michael K. Riscoe","orcid":"https://orcid.org/0000-0002-1343-5279","observed_orcids":["https://orcid.org/0000-0002-1343-5279"]},"institutions":[{"id":"https://openalex.org/I126345244","display_name":"Portland State University","ror":"https://ror.org/00yn2fy02","country_code":"US","type":"education","lineage":["https://openalex.org/I126345244"]},{"id":"https://openalex.org/I2799886695","display_name":"Veterans Health Administration","ror":"https://ror.org/05eq41471","country_code":"US","type":"government","lineage":["https://openalex.org/I1322918889","https://openalex.org/I2799886695"]},{"id":"https://openalex.org/I2803065977","display_name":"Portland VA Medical Center","ror":"https://ror.org/02v3txv81","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1322918889","https://openalex.org/I2799886695","https://openalex.org/I2803065977","https://openalex.org/I4210144745"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michael K. Riscoe","raw_affiliation_strings":["Department of Chemistry, Portland State University, Portland, Oregon 97207","Medical Research Service, RD-33, Veterans Affairs Medical Center, Portland, Oregon 97021, and the"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Portland State University, Portland, Oregon 97207","institution_ids":["https://openalex.org/I126345244"]},{"raw_affiliation_string":"Medical Research Service, RD-33, Veterans Affairs Medical Center, Portland, Oregon 97021, and the","institution_ids":["https://openalex.org/I2799886695","https://openalex.org/I2803065977"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5018522950","display_name":"Vern L. Schramm","orcid":"https://orcid.org/0000-0002-8056-1929","observed_orcids":["https://orcid.org/0000-0002-8056-1929"]},"institutions":[{"id":"https://openalex.org/I129975664","display_name":"Albert Einstein College of Medicine","ror":"https://ror.org/05cf8a891","country_code":"US","type":"education","lineage":["https://openalex.org/I129975664","https://openalex.org/I4210112371"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Vern L. Schramm","raw_affiliation_strings":["Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461, the"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Albert Einstein College of Medicine, Bronx, New York 10461, the","institution_ids":["https://openalex.org/I129975664"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5011961609","display_name":"P. Lynne Howell","orcid":"https://orcid.org/0000-0002-2776-062X","observed_orcids":["https://orcid.org/0000-0002-2776-062X"]},"institutions":[{"id":"https://openalex.org/I185261750","display_name":"University of Toronto","ror":"https://ror.org/03dbr7087","country_code":"CA","type":"education","lineage":["https://openalex.org/I185261750"]},{"id":"https://openalex.org/I2801317318","display_name":"Hospital for Sick Children","ror":"https://ror.org/057q4rt57","country_code":"CA","type":"healthcare","lineage":["https://openalex.org/I2801317318"]}],"countries":["CA"],"is_corresponding":false,"raw_author_name":"P. Lynne Howell","raw_affiliation_strings":["Department of Biochemistry, Faculty of Medicine, University of Toronto, Medical Sciences Building, Toronto, Ontario M5S 1A8, Canada, the","Structural Biology and Biochemistry, Research Institute, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada, the"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Faculty of Medicine, University of Toronto, Medical Sciences Building, Toronto, Ontario M5S 1A8, Canada, the","institution_ids":["https://openalex.org/I185261750"]},{"raw_affiliation_string":"Structural Biology and Biochemistry, Research Institute, Hospital for Sick Children, Toronto, Ontario M5G 1X8, Canada, the","institution_ids":["https://openalex.org/I2801317318"]}]}],"institutions":[],"countries_distinct_count":3,"institutions_distinct_count":8,"corresponding_author_ids":["https://openalex.org/A5102846930"],"corresponding_institution_ids":["https://openalex.org/I185261750","https://openalex.org/I2801317318"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":2.4778,"has_fulltext":true,"cited_by_count":75,"citation_normalized_percentile":{"value":0.89631541,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":99},"biblio":{"volume":"280","issue":"18","first_page":"18274","last_page":"18282"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12827","display_name":"Biochemical and Molecular Research","score":0.989300012588501,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12827","display_name":"Biochemical and Molecular Research","score":0.989300012588501,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12191","display_name":"Physical Organic Chemistry and Reaction Kinetics","score":0.005400000140070915,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.0010999999940395355,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/transition-state-analogs","display_name":"transition state analogues","score":0.8109999895095825},{"id":"https://openalex.org/keywords/purine-nucleoside-phosphorylase","display_name":"purine nucleoside phosphorylase","score":0.8230000138282776},{"id":"https://openalex.org/keywords/enzyme-inhibition","display_name":"enzyme inhibition","score":0.5370000004768372},{"id":"https://openalex.org/keywords/crystal-structure","display_name":"crystal structure","score":0.6890000104904175},{"id":"https://openalex.org/keywords/tight-binding-inhibitors","display_name":"tight-binding inhibitors","score":0.5090000033378601},{"id":"https://openalex.org/keywords/ion-pair-interactions","display_name":"ion-pair interactions","score":0.6240000128746033},{"id":"https://openalex.org/keywords/hydrogen-bonding","display_name":"hydrogen bonding","score":0.7609999775886536},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.7749999761581421}],"concepts":[{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.7467721700668335},{"id":"https://openalex.org/C13053905","wikidata":"https://www.wikidata.org/wiki/Q17087414","display_name":"Transition state analog","level":4,"score":0.6637640595436096},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.6195544600486755},{"id":"https://openalex.org/C71615608","wikidata":"https://www.wikidata.org/wiki/Q24720082","display_name":"Purine nucleoside phosphorylase","level":4,"score":0.46864956617355347},{"id":"https://openalex.org/C194232998","wikidata":"https://www.wikidata.org/wiki/Q1606712","display_name":"Transition (genetics)","level":3,"score":0.4465234875679016},{"id":"https://openalex.org/C116569031","wikidata":"https://www.wikidata.org/wiki/Q899107","display_name":"Ligand (biochemistry)","level":3,"score":0.41067203879356384},{"id":"https://openalex.org/C8010536","wikidata":"https://www.wikidata.org/wiki/Q160398","display_name":"Crystallography","level":1,"score":0.4042074680328369},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.3984057605266571},{"id":"https://openalex.org/C2776476023","wikidata":"https://www.wikidata.org/wiki/Q188261","display_name":"Purine","level":3,"score":0.2460728883743286},{"id":"https://openalex.org/C41183919","wikidata":"https://www.wikidata.org/wiki/Q423026","display_name":"Active site","level":3,"score":0.23366403579711914},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.18744605779647827},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.06747046113014221},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D003839","descriptor_name":"Deoxyadenosines","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D003839","descriptor_name":"Deoxyadenosines","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D004791","descriptor_name":"Enzyme Inhibitors","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D008958","descriptor_name":"Models, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D009699","descriptor_name":"N-Glycosyl Hydrolases","qualifier_ui":"Q000037","qualifier_name":"antagonists & inhibitors","is_major_topic":false},{"descriptor_ui":"D009699","descriptor_name":"N-Glycosyl Hydrolases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D009699","descriptor_name":"N-Glycosyl Hydrolases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D011684","descriptor_name":"Purine Nucleosides","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011744","descriptor_name":"Pyrimidinones","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011758","descriptor_name":"Pyrroles","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D013872","descriptor_name":"Thionucleosides","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013872","descriptor_name":"Thionucleosides","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.1074/jbc.m414471200","doi":"https://doi.org/10.1074/jbc.m414471200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m414471200","pdf_url":"http://www.jbc.org/article/S0021925820657763/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:15746096","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/15746096","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1074/jbc.m414471200","doi":"https://doi.org/10.1074/jbc.m414471200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m414471200","pdf_url":"http://www.jbc.org/article/S0021925820657763/pdf","source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G6096675853","display_name":null,"funder_award_id":"43998","funder_id":"https://openalex.org/F4320334506","funder_display_name":"Canadian Institutes of Health Research"}],"funders":[{"id":"https://openalex.org/F4320306114","display_name":"U.S. Department of Agriculture","ror":"https://ror.org/01na82s61"},{"id":"https://openalex.org/F4320306127","display_name":"U.S. Department of Veterans Affairs","ror":"https://ror.org/05rsv9s98"},{"id":"https://openalex.org/F4320323654","display_name":"Hospital for Sick Children","ror":"https://ror.org/057q4rt57"},{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320334506","display_name":"Canadian Institutes of Health Research","ror":"https://ror.org/01gavpb45"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2127394656.pdf","grobid_xml":"https://content.openalex.org/works/W2127394656.grobid-xml"},"referenced_works_count":62,"referenced_works":["https://openalex.org/W184495677","https://openalex.org/W636053674","https://openalex.org/W1483016487","https://openalex.org/W1513354202","https://openalex.org/W1527275481","https://openalex.org/W1964506904","https://openalex.org/W1964803671","https://openalex.org/W1966964730","https://openalex.org/W1970327093","https://openalex.org/W1972730956","https://openalex.org/W1973058081","https://openalex.org/W1986191025","https://openalex.org/W1988961476","https://openalex.org/W1991804063","https://openalex.org/W1994100849","https://openalex.org/W1994462493","https://openalex.org/W1995017064","https://openalex.org/W1998326947","https://openalex.org/W2001569378","https://openalex.org/W2004080823","https://openalex.org/W2012322250","https://openalex.org/W2014804052","https://openalex.org/W2022784572","https://openalex.org/W2022889821","https://openalex.org/W2024249829","https://openalex.org/W2028531632","https://openalex.org/W2029667189","https://openalex.org/W2040317364","https://openalex.org/W2042666860","https://openalex.org/W2042933128","https://openalex.org/W2051881223","https://openalex.org/W2055989425","https://openalex.org/W2060448324","https://openalex.org/W2061774324","https://openalex.org/W2064013612","https://openalex.org/W2064589373","https://openalex.org/W2067887310","https://openalex.org/W2073760752","https://openalex.org/W2080528351","https://openalex.org/W2084213291","https://openalex.org/W2084648582","https://openalex.org/W2086058162","https://openalex.org/W2086210103","https://openalex.org/W2087989446","https://openalex.org/W2092002806","https://openalex.org/W2094186244","https://openalex.org/W2094586236","https://openalex.org/W2095263150","https://openalex.org/W2095496612","https://openalex.org/W2097147953","https://openalex.org/W2105279142","https://openalex.org/W2105844209","https://openalex.org/W2110546335","https://openalex.org/W2116947993","https://openalex.org/W2130479394","https://openalex.org/W2135839939","https://openalex.org/W2144640625","https://openalex.org/W2152300317","https://openalex.org/W2160661817","https://openalex.org/W2244924092","https://openalex.org/W2504337932","https://openalex.org/W2949277270"],"related_works":["https://openalex.org/W2152300317","https://openalex.org/W2042933128","https://openalex.org/W2001569378","https://openalex.org/W4239340372","https://openalex.org/W1990397959","https://openalex.org/W2083367125","https://openalex.org/W2011721714","https://openalex.org/W2075105792","https://openalex.org/W2010294108","https://openalex.org/W2070818058"],"abstract_inverted_index":{"Immucillin":[0,36],"and":[1,37,48,93,106,117,121,148,156,177,209,240,252],"DADMe-Immucillin":[2,38],"inhibitors":[3,39],"are":[4,168],"tight":[5,245],"binding":[6,172,183],"transition":[7,24,43,135,220,264,276],"state":[8,25,44,136,166,277],"mimics":[9],"of":[10,34,46,115,131,134,141,179,182,200,218,249,261,272,278],"purine":[11],"nucleoside":[12],"phosphorylases":[13],"(PNP).":[14],"5'-Methylthioadenosine/S-adenosylhomocysteine":[15],"nucleosidase":[16],"(MTAN)":[17],"is":[18,242,258,268],"proposed":[19],"to":[20,40,51,60,152],"form":[21],"a":[22,201,227,259,269],"similar":[23],"structure":[26],"as":[27,127],"PNP.":[28],"The":[29,138,194],"companion":[30],"paper":[31],"describes":[32],"modifications":[33],"the":[35,57,61,128,159,170,188,198,206,210,219,224,236,250,273],"better":[41,270],"match":[42],"features":[45,217],"MTAN":[47,144,161],"have":[49],"led":[50],"5'-thio":[52],"aromatic":[53],"substitutions":[54],"that":[55,214,256],"extend":[56],"inhibition":[58,112],"constants":[59],"femtomolar":[62],"range":[63],"(Singh,":[64],"V.,":[65],"Evans,":[66],"G.":[67,79],"B.,":[68],"Lenz,":[69],"D.":[70],"H.,":[71,86],"Mason,":[72],"J.,":[73],"Clinch,":[74],"K.,":[75],"Mee,":[76],"S.,":[77],"Painter,":[78],"F.,":[80],"Tyler,":[81],"P.":[82,91],"C.,":[83],"Furneaux,":[84],"R.":[85],"Lee,":[87],"J.":[88,98],"E.,":[89],"Howell,":[90],"L.,":[92],"Schramm,":[94],"V.":[95],"L.":[96],"(2005)":[97],"Biol.":[99],"Chem.":[100],"280,":[101],"18265-18273).":[102],"5'-Methylthio-Immucillin":[103],"A":[104,108,154],"(MT-ImmA)":[105],"5'-methylthio-DADMe-Immucillin":[107],"(MT-DADMe-ImmA)":[109],"exhibit":[110],"slow-onset":[111],"with":[113,146,158],"K(i)(*)":[114],"77":[116],"2":[118],"pm,":[119],"respectively,":[120],"were":[122,150],"selected":[123],"for":[124,191,244],"structural":[125,189],"analysis":[126,178,248],"parent":[129],"compounds":[130],"each":[132],"class":[133],"analogue.":[137],"crystal":[139],"structures":[140],"Escherichia":[142],"coli":[143,280],"complexed":[145],"MT-ImmA":[147,257],"MT-DADMe-ImmA":[149,267],"determined":[151],"2.2":[153],"resolution":[155],"compared":[157],"existing":[160],"inhibitor":[162],"complexes.":[163],"These":[164],"MTAN-transition":[165],"complexes":[167,174],"among":[169],"tightest":[171],"enzyme-ligand":[173],"ever":[175],"described":[176],"their":[180,192],"mode":[181],"provides":[184],"extraordinary":[185],"insight":[186],"into":[187],"basis":[190],"affinity.":[193,246],"MTAN-MT-ImmA":[195],"complex":[196,226],"reveals":[197],"presence":[199],"new":[202],"ion":[203,232],"pair":[204,233],"between":[205,235],"4'-iminoribitol":[207],"atom":[208,239],"nucleophilic":[211],"water":[212],"(WAT3)":[213],"captures":[215],"key":[216],"state.":[221],"Similarly,":[222],"in":[223],"MTAN-MT-DADMe-ImmA":[225],"favorable":[228],"hydrogen":[229],"bond":[230],"or":[231],"interaction":[234],"cationic":[237],"1'-pyrrolidine":[238],"WAT3":[241],"crucial":[243],"Distance":[247],"nucleophile":[251],"leaving":[253],"group":[254],"show":[255],"mimic":[260,271],"an":[262],"early":[263],"state,":[265],"while":[266],"highly":[274],"dissociated":[275],"E.":[279],"MTAN.":[281]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":4},{"year":2018,"cited_by_count":2},{"year":2017,"cited_by_count":7},{"year":2016,"cited_by_count":2},{"year":2015,"cited_by_count":5},{"year":2014,"cited_by_count":5},{"year":2013,"cited_by_count":3},{"year":2012,"cited_by_count":6}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}
