{"id":"https://openalex.org/W2126475188","doi":null,"title":"The contribution of proteins and lipids to COPI vesicle formation and consumption","display_name":"The contribution of proteins and lipids to COPI vesicle formation and consumption","publication_year":2008,"publication_date":"2008-11-18","ids":{"openalex":"https://openalex.org/W2126475188","mag":"2126475188"},"language":"en","primary_location":{"id":"pmh:oai:gupea.ub.gu.se:2077/18716","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/2077/18716","pdf_url":"http://hdl.handle.net/2077/18716","source":{"id":"https://openalex.org/S4306401168","display_name":"Gothenburg University Publications Electronic Archive (Gothenburg University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I881427289","host_organization_name":"University of Gothenburg","host_organization_lineage":["https://openalex.org/I881427289"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Doctoral thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://hdl.handle.net/2077/18716","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5000193655","display_name":"Fredrik Kartberg","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Fredrik Kartberg","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5000193655"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":true,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11844","display_name":"Lipid metabolism and biosynthesis","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10169","display_name":"Protein Kinase Regulation and GTPase Signaling","score":0.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/copi-vesicles","display_name":"COPI vesicles","score":0.9279999732971191},{"id":"https://openalex.org/keywords/vesicle-budding","display_name":"vesicle budding","score":0.7480000257492065},{"id":"https://openalex.org/keywords/arf-gtpases","display_name":"Arf GTPases","score":0.7160000205039978},{"id":"https://openalex.org/keywords/arfgap1","display_name":"ArfGAP1","score":0.9279999732971191},{"id":"https://openalex.org/keywords/diacylglycerol","display_name":"diacylglycerol","score":0.878000020980835},{"id":"https://openalex.org/keywords/coatomer","display_name":"coatomer","score":0.6890000104904175},{"id":"https://openalex.org/keywords/golgi-to-er-transport","display_name":"Golgi-to-ER transport","score":0.8169999718666077},{"id":"https://openalex.org/keywords/vesicle-docking-and-fusion","display_name":"vesicle docking and fusion","score":0.7860000133514404},{"id":"https://openalex.org/keywords/snare-proteins","display_name":"SNARE proteins","score":0.6340000033378601},{"id":"https://openalex.org/keywords/vamp3","display_name":"VAMP3","score":0.3409999907016754},{"id":"https://openalex.org/keywords/vamp4","display_name":"VAMP4","score":0.5099999904632568},{"id":"https://openalex.org/keywords/vamp7","display_name":"VAMP7","score":0.4819999933242798},{"id":"https://openalex.org/keywords/vamp8","display_name":"VAMP8","score":0.4480000138282776},{"id":"https://openalex.org/keywords/secretory-pathway","display_name":"secretory pathway","score":0.6549999713897705},{"id":"https://openalex.org/keywords/membrane-lipid-composition","display_name":"membrane lipid composition","score":0.3140000104904175},{"id":"https://openalex.org/keywords/phosphatidylinositol-3-phosphate","display_name":"phosphatidylinositol 3-phosphate","score":0.6259999871253967}],"concepts":[{"id":"https://openalex.org/C130316041","wikidata":"https://www.wikidata.org/wiki/Q189206","display_name":"Vesicle","level":3,"score":0.8885462880134583},{"id":"https://openalex.org/C206912223","wikidata":"https://www.wikidata.org/wiki/Q21111963","display_name":"COPI","level":5,"score":0.8170150518417358},{"id":"https://openalex.org/C119062480","wikidata":"https://www.wikidata.org/wiki/Q83181","display_name":"Golgi apparatus","level":3,"score":0.6857141852378845},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.6817342042922974},{"id":"https://openalex.org/C6492254","wikidata":"https://www.wikidata.org/wiki/Q84230","display_name":"Secretory pathway","level":4,"score":0.6080339550971985},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.5343550443649292},{"id":"https://openalex.org/C198410946","wikidata":"https://www.wikidata.org/wiki/Q845312","display_name":"Budding","level":2,"score":0.5004768371582031},{"id":"https://openalex.org/C46617944","wikidata":"https://www.wikidata.org/wiki/Q410907","display_name":"COPII","level":5,"score":0.49779558181762695},{"id":"https://openalex.org/C196330066","wikidata":"https://www.wikidata.org/wiki/Q7923216","display_name":"Vesicle fusion","level":5,"score":0.42102983593940735},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.36472606658935547},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3093557357788086},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.2813238501548767},{"id":"https://openalex.org/C158617107","wikidata":"https://www.wikidata.org/wiki/Q79927","display_name":"Endoplasmic reticulum","level":2,"score":0.12511694431304932},{"id":"https://openalex.org/C148785051","wikidata":"https://www.wikidata.org/wiki/Q671136","display_name":"Synaptic vesicle","level":4,"score":0.09634467959403992}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:gupea.ub.gu.se:2077/18716","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/2077/18716","pdf_url":"http://hdl.handle.net/2077/18716","source":{"id":"https://openalex.org/S4306401168","display_name":"Gothenburg University Publications Electronic Archive (Gothenburg University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I881427289","host_organization_name":"University of Gothenburg","host_organization_lineage":["https://openalex.org/I881427289"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Doctoral thesis"},{"id":"mag:2126475188","doi":null,"is_oa":false,"landing_page_url":"https://gupea.ub.gu.se/bitstream/2077/18716/1/gupea_2077_18716_1.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":{"id":"pmh:oai:gupea.ub.gu.se:2077/18716","doi":null,"is_oa":true,"landing_page_url":"http://hdl.handle.net/2077/18716","pdf_url":"http://hdl.handle.net/2077/18716","source":{"id":"https://openalex.org/S4306401168","display_name":"Gothenburg University Publications Electronic Archive (Gothenburg University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I881427289","host_organization_name":"University of Gothenburg","host_organization_lineage":["https://openalex.org/I881427289"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Doctoral thesis"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2126475188.pdf","grobid_xml":"https://content.openalex.org/works/W2126475188.grobid-xml"},"referenced_works_count":271,"referenced_works":["https://openalex.org/W1488601217","https://openalex.org/W1496815279","https://openalex.org/W1499112972","https://openalex.org/W1504892441","https://openalex.org/W1516922966","https://openalex.org/W1518401857","https://openalex.org/W1531818302","https://openalex.org/W1535587662","https://openalex.org/W1536827165","https://openalex.org/W1553644521","https://openalex.org/W1554520293","https://openalex.org/W1556943982","https://openalex.org/W1560207274","https://openalex.org/W1580161657","https://openalex.org/W1585607520","https://openalex.org/W1585675237","https://openalex.org/W1589334735","https://openalex.org/W1672154570","https://openalex.org/W1693383484","https://openalex.org/W1754492371","https://openalex.org/W1844204379","https://openalex.org/W1902362655","https://openalex.org/W1943315667","https://openalex.org/W1963573377","https://openalex.org/W1964660197","https://openalex.org/W1965448789","https://openalex.org/W1966190533","https://openalex.org/W1966990402","https://openalex.org/W1967131697","https://openalex.org/W1968733935","https://openalex.org/W1971374133","https://openalex.org/W1971548708","https://openalex.org/W1971715401","https://openalex.org/W1972510078","https://openalex.org/W1972523391","https://openalex.org/W1972948209","https://openalex.org/W1973460023","https://openalex.org/W1973642611","https://openalex.org/W1976579206","https://openalex.org/W1977631266","https://openalex.org/W1977903940","https://openalex.org/W1978701884","https://openalex.org/W1979742234","https://openalex.org/W1980330613","https://openalex.org/W1980856167","https://openalex.org/W1981503496","https://openalex.org/W1983127906","https://openalex.org/W1984311414","https://openalex.org/W1984730682","https://openalex.org/W1985349036","https://openalex.org/W1985700603","https://openalex.org/W1986616082","https://openalex.org/W1988752034","https://openalex.org/W1989189680","https://openalex.org/W1989479561","https://openalex.org/W1990130046","https://openalex.org/W1990349821","https://openalex.org/W1990725668","https://openalex.org/W1994624166","https://openalex.org/W1994838572","https://openalex.org/W1995164025","https://openalex.org/W1995490800","https://openalex.org/W1996868880","https://openalex.org/W1997851570","https://openalex.org/W2000302106","https://openalex.org/W2000504183","https://openalex.org/W2000647007","https://openalex.org/W2001592340","https://openalex.org/W2001888120","https://openalex.org/W2003123776","https://openalex.org/W2005100357","https://openalex.org/W2005672588","https://openalex.org/W2007483105","https://openalex.org/W2008009726","https://openalex.org/W2008597859","https://openalex.org/W2009209652","https://openalex.org/W2009468127","https://openalex.org/W2009616915","https://openalex.org/W2010055171","https://openalex.org/W2010228789","https://openalex.org/W2011483335","https://openalex.org/W2011485864","https://openalex.org/W2012484113","https://openalex.org/W2012614643","https://openalex.org/W2013314128","https://openalex.org/W2013578233","https://openalex.org/W2013774412","https://openalex.org/W2014069822","https://openalex.org/W2014164653","https://openalex.org/W2015256188","https://openalex.org/W2016417523","https://openalex.org/W2018415759","https://openalex.org/W2019348717","https://openalex.org/W2019591531","https://openalex.org/W2022743955","https://openalex.org/W2022977995","https://openalex.org/W2023397454","https://openalex.org/W2026039614","https://openalex.org/W2027371255","https://openalex.org/W2028675060","https://openalex.org/W2028798604","https://openalex.org/W2029350327","https://openalex.org/W2029581399","https://openalex.org/W2029922958","https://openalex.org/W2030111014","https://openalex.org/W2030257970","https://openalex.org/W2030648310","https://openalex.org/W2032409654","https://openalex.org/W2033053971","https://openalex.org/W2035807916","https://openalex.org/W2037401195","https://openalex.org/W2037582316","https://openalex.org/W2038422914","https://openalex.org/W2038570103","https://openalex.org/W2039170982","https://openalex.org/W2041478064","https://openalex.org/W2041813726","https://openalex.org/W2043913863","https://openalex.org/W2044137708","https://openalex.org/W2044323236","https://openalex.org/W2046332353","https://openalex.org/W2046356716","https://openalex.org/W2048041152","https://openalex.org/W2049900761","https://openalex.org/W2049912462","https://openalex.org/W2050199375","https://openalex.org/W2050370363","https://openalex.org/W2051246945","https://openalex.org/W2052332314","https://openalex.org/W2052629714","https://openalex.org/W2052949000","https://openalex.org/W2052992979","https://openalex.org/W2054593368","https://openalex.org/W2056283979","https://openalex.org/W2056748148","https://openalex.org/W2057671138","https://openalex.org/W2058210463","https://openalex.org/W2058970378","https://openalex.org/W2060528642","https://openalex.org/W2061517276","https://openalex.org/W2061669611","https://openalex.org/W2062819360","https://openalex.org/W2063005214","https://openalex.org/W2064880862","https://openalex.org/W2066221480","https://openalex.org/W2066574113","https://openalex.org/W2070131512","https://openalex.org/W2070136749","https://openalex.org/W2070706683","https://openalex.org/W2070992410","https://openalex.org/W2071725693","https://openalex.org/W2071792089","https://openalex.org/W2072167784","https://openalex.org/W2073673210","https://openalex.org/W2074175128","https://openalex.org/W2075631488","https://openalex.org/W2075967815","https://openalex.org/W2077416083","https://openalex.org/W2077513922","https://openalex.org/W2077817300","https://openalex.org/W2078021971","https://openalex.org/W2078959581","https://openalex.org/W2079268511","https://openalex.org/W2079613065","https://openalex.org/W2080987586","https://openalex.org/W2082387821","https://openalex.org/W2086008461","https://openalex.org/W2086084527","https://openalex.org/W2086447896","https://openalex.org/W2086562476","https://openalex.org/W2086620317","https://openalex.org/W2086907495","https://openalex.org/W2087165198","https://openalex.org/W2087388355","https://openalex.org/W2087449196","https://openalex.org/W2089039194","https://openalex.org/W2092791905","https://openalex.org/W2094037158","https://openalex.org/W2094372926","https://openalex.org/W2094612102","https://openalex.org/W2094997676","https://openalex.org/W2096858027","https://openalex.org/W2096947423","https://openalex.org/W2098436412","https://openalex.org/W2098607413","https://openalex.org/W2100487411","https://openalex.org/W2101275009","https://openalex.org/W2102226328","https://openalex.org/W2102285200","https://openalex.org/W2102948276","https://openalex.org/W2103001017","https://openalex.org/W2103593226","https://openalex.org/W2104119889","https://openalex.org/W2104152706","https://openalex.org/W2104981213","https://openalex.org/W2105208936","https://openalex.org/W2105642340","https://openalex.org/W2106076932","https://openalex.org/W2106141363","https://openalex.org/W2106813748","https://openalex.org/W2109185500","https://openalex.org/W2110412999","https://openalex.org/W2111525004","https://openalex.org/W2111620983","https://openalex.org/W2112078762","https://openalex.org/W2112122957","https://openalex.org/W2112488154","https://openalex.org/W2112921524","https://openalex.org/W2113121382","https://openalex.org/W2113343156","https://openalex.org/W2113418681","https://openalex.org/W2115112794","https://openalex.org/W2115191599","https://openalex.org/W2115637042","https://openalex.org/W2117761303","https://openalex.org/W2118286268","https://openalex.org/W2118515161","https://openalex.org/W2119441048","https://openalex.org/W2119894822","https://openalex.org/W2122057086","https://openalex.org/W2123151328","https://openalex.org/W2124248825","https://openalex.org/W2127432085","https://openalex.org/W2128975869","https://openalex.org/W2129381290","https://openalex.org/W2132062710","https://openalex.org/W2132520995","https://openalex.org/W2132569852","https://openalex.org/W2133488205","https://openalex.org/W2134654436","https://openalex.org/W2134708980","https://openalex.org/W2134827549","https://openalex.org/W2136295938","https://openalex.org/W2136345561","https://openalex.org/W2136617021","https://openalex.org/W2136767661","https://openalex.org/W2137032962","https://openalex.org/W2137516675","https://openalex.org/W2139868649","https://openalex.org/W2140936469","https://openalex.org/W2141521022","https://openalex.org/W2144661465","https://openalex.org/W2144918914","https://openalex.org/W2144944458","https://openalex.org/W2145835877","https://openalex.org/W2146568672","https://openalex.org/W2146850365","https://openalex.org/W2148114086","https://openalex.org/W2148967117","https://openalex.org/W2152561526","https://openalex.org/W2152708819","https://openalex.org/W2152719012","https://openalex.org/W2156816000","https://openalex.org/W2158019890","https://openalex.org/W2159362830","https://openalex.org/W2163019509","https://openalex.org/W2163560348","https://openalex.org/W2166247822","https://openalex.org/W2167988140","https://openalex.org/W2167994809","https://openalex.org/W2169151877","https://openalex.org/W2170030312","https://openalex.org/W2171495269","https://openalex.org/W2171810148","https://openalex.org/W2181827540","https://openalex.org/W2183308532","https://openalex.org/W2184457366","https://openalex.org/W2339438128","https://openalex.org/W2424250565","https://openalex.org/W2952450708","https://openalex.org/W3142567747"],"related_works":["https://openalex.org/W2017475690","https://openalex.org/W2046756919","https://openalex.org/W1964660197","https://openalex.org/W2022743955","https://openalex.org/W2091482320","https://openalex.org/W1970954184","https://openalex.org/W1969312560","https://openalex.org/W2009087844","https://openalex.org/W2170752133","https://openalex.org/W2085392052"],"abstract_inverted_index":{"In":[0,31,183],"the":[1,22,27,32,50,55,66,74,78,88,91,106,109,125,128,135,139,188,221,254,267,270,285,308,323,350,354,358,374,387,417,425,433,474,486,489],"secretory":[2,33],"pathway,":[3,34],"movement":[4],"of":[5,17,46,90,108,121,127,134,145,158,162,169,178,187,209,223,238,243,248,258,266,280,289,295,302,317,322,362,376,389,395,411,432,476],"proteins":[6],"between":[7],"compartments":[8],"occurs":[9],"through":[10],"small":[11,23],"(50-100":[12],"nm)":[13],"vesicles.":[14,173,198,392],"The":[15,287,304,393,409],"coat":[16,310,332],"COPI":[18,100,228,309,346,363],"vesicles":[19,36,364,396,423],"is":[20,203,214,384],"composed":[21],"GTPase":[24],"ARF1":[25,53,412],"and":[26,39,84,103,171,251,282,325,360,402,437],"large":[28],"coatomer":[29,48,252],"complex.":[30],"these":[35,338],"mediate":[37],"Golgi-to-ER":[38],"intra-Golgi":[40,377],"transport.":[41,378],"During":[42,72],"vesicle":[43,75,101,229,239,347,382],"formation,":[44],"recruitment":[45],"cytosolic":[47],"to":[49,77,99,293,306,413],"membrane":[51,68,194,419],"by":[52,82,124,241,386,442],"represents":[54],"key":[56],"step.":[57],"This":[58,94,199,335,467],"generates":[59],"a":[60,70,166,217,298,398,405,430],"bud,":[61],"which":[62],"subsequently":[63],"separates":[64],"from":[65,138],"donor":[67],"as":[69,479],"vesicle.":[71],"consumption,":[73],"tethers":[76],"target":[79],"membrane,":[80],"followed":[81],"docking":[83,359],"fusion,":[85],"resulting":[86],"in":[87,113,153,165,193,227,231,312,345,349,370,453,463],"merging":[89],"two":[92,224,339],"bilayers.":[93],"thesis":[95],"has":[96],"been":[97],"devoted":[98],"formation":[102,211,230,348],"consumption.\\n\\nWe":[104],"examined":[105],"role":[107,300,344],"lipid":[110],"diacylglycerol":[111],"(DAG)":[112],"bud":[114,210],"formation.":[115,334],"We":[116,234,379,446],"demonstrate":[117,235],"that":[118,150,201,236,273,294,337,356,381,448,481],"efficient":[119],"inhibition":[120],"DAG":[122,202],"synthesis":[123],"addition":[126,242],"inhibitor":[129,189],"Propranolol":[130,163],"causes":[131,246],"rapid":[132],"dissociation":[133],"ARFGAP1":[136,179,213,330],"protein":[137],"membrane.":[140,286,490],"Upon":[141],"electron":[142],"microscopy":[143],"examinations":[144],"treated":[146,181],"cells,":[147,185],"we":[148],"find":[149,380,447],"this":[151,274],"results":[152],"smooth":[154],"Golgi":[155,271,366,418],"membranes":[156],"devoid":[157],"budding":[159,240],"profiles.":[160],"Washout":[161],"resulted":[164],"marked":[167],"increase":[168,192],"buds":[170,195],"associated":[172],"Cells":[174],"expressing":[175],"low":[176],"amounts":[177],"were":[180],"similarly.":[182],"such":[184,478],"removal":[186],"caused":[190],"an":[191,206,342,369],"but":[196,256,328,455],"not":[197,257,329],"suggests":[200,336],"needed":[204],"at":[205,216],"early":[207],"stage":[208],"whereas":[212],"required":[215],"later":[218],"step.\\n\\nWe":[219],"investigated":[220],"function":[222,475],"new":[225],"ARFGAPs":[226,340],"living":[232],"cells.":[233],"stimulation":[237],"aluminum":[244],"fluoride":[245],"accumulation":[247,275],"ARFGAP2,":[249],"ARFGAP3,":[250],"on":[253,391,416,483],"Golgi,":[255],"ARFGAP1.":[259,303],"Fluorescence":[260],"recovery":[261],"after":[262],"photobleaching":[263],"(FRAP)":[264],"analysis":[265],"association":[268,487],"with":[269,284,365,397,404,488],"demonstrates":[272],"also":[276],"reflects":[277],"irreversible":[278],"binding":[279],"ARFGAP2":[281,324],"ARFGAP3":[283,326],"degree":[288],"immobilization":[290],"was":[291,319],"close":[292],"coatomer,":[296,480],"suggesting":[297],"closer":[299],"than":[301],"ability":[305,410],"generate":[307,414],"lattice":[311,333],"cells":[313,461],"lacking":[314],"different":[315],"combinations":[316],"ARFGAP1-3":[318],"investigated.":[320],"Absence":[321],"pair":[327],"prevented":[331],"play":[341],"overlapping":[343],"Golgi.\\n\\nWe":[351],"looked":[352],"into":[353,460],"factors":[355],"influence":[357],"fusion":[361,383,401,427],"cisternae":[367],"using":[368],"vitro":[371],"assay":[372],"for":[373,424,440,473,485],"reconstitution":[375],"regulated":[385],"presence":[388],"PI(4,5)P2":[390,415],"pre-treatment":[394],"kinase":[399],"stimulated":[400],"treatment":[403],"phosphatase":[406],"inhibited":[407],"fusion.":[408],"may":[420],"therefore":[421],"prime":[422],"following":[426],"event.\\n\\nWe":[428],"analyzed":[429],"property":[431],"cytosol,":[434],"molecular":[435],"crowding,":[436],"its":[438],"consequences":[439,472],"diffusion":[441,484],"fluorescence":[443],"correlation":[444],"spectroscopy.":[445],"fluorescent":[449],"dextrans":[450],"diffuse":[451],"normally":[452],"water":[454],"become":[456],"subdiffusive":[457],"upon":[458],"microinjection":[459],"or":[462],"artificially":[464],"crowded":[465],"solutions.":[466],"phenomenon":[468],"can":[469],"have":[470],"important":[471],"proteins,":[477],"depend":[482]},"counts_by_year":[],"updated_date":"2026-08-29T07:29:34.045763","created_date":"2016-06-24T00:00:00"}
