{"id":"https://openalex.org/W2124366355","doi":"https://doi.org/10.1093/nar/gkl917","title":"Use of signal sequences as an in situ removable sequence element to stimulate protein synthesis in cell-free extracts","display_name":"Use of signal sequences as an in situ removable sequence element to stimulate protein synthesis in cell-free extracts","publication_year":2006,"publication_date":"2006-12-22","ids":{"openalex":"https://openalex.org/W2124366355","doi":"https://doi.org/10.1093/nar/gkl917","mag":"2124366355","pmid":"https://pubmed.ncbi.nlm.nih.gov/17185295","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/1849898"},"language":"en","primary_location":{"id":"doi:10.1093/nar/gkl917","doi":"https://doi.org/10.1093/nar/gkl917","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/gkl917","pdf_url":"https://academic.oup.com/nar/article-pdf/35/4/e21/4916234/gkl917.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://academic.oup.com/nar/article-pdf/35/4/e21/4916234/gkl917.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5058197414","display_name":"Junho Ahn","orcid":"https://orcid.org/0000-0002-1903-0248","observed_orcids":["https://orcid.org/0000-0002-1903-0248"]},"institutions":[{"id":"https://openalex.org/I139264467","display_name":"Seoul National University","ror":"https://ror.org/04h9pn542","country_code":"KR","type":"education","lineage":["https://openalex.org/I139264467"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"J.-H. Ahn","raw_affiliation_strings":["Interdisciplinary Program for Biochemical Engineering and Biotechnology, College of Engineering, Seoul National University, Seoul 151-742, Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Interdisciplinary Program for Biochemical Engineering and Biotechnology, College of Engineering, Seoul National University, Seoul 151-742, Korea","institution_ids":["https://openalex.org/I139264467"]}]},{"author_position":"middle","author":{"id":null,"display_name":"M.-Y. Hwang","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I139264467","display_name":"Seoul National University","ror":"https://ror.org/04h9pn542","country_code":"KR","type":"education","lineage":["https://openalex.org/I139264467"]},{"id":"https://openalex.org/I196345858","display_name":"Chungnam National University","ror":"https://ror.org/0227as991","country_code":"KR","type":"education","lineage":["https://openalex.org/I196345858"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"M.-Y. Hwang","raw_affiliation_strings":["Interdisciplinary Program for Biochemical Engineering and Biotechnology, College of Engineering, Seoul National University, Seoul 151-742, Korea, 2 School of Chemical and Biological Engineering, College of Engineering, Seoul National University, Seoul 151-742, Korea and 3 Department of Fine Chemical Engineering and Chemistry, Chungnam National University, Daejeon 305-764, Korea","Chungnam National University, Daejeon, South Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Interdisciplinary Program for Biochemical Engineering and Biotechnology, College of Engineering, Seoul National University, Seoul 151-742, Korea, 2 School of Chemical and Biological Engineering, College of Engineering, Seoul National University, Seoul 151-742, Korea and 3 Department of Fine Chemical Engineering and Chemistry, Chungnam National University, Daejeon 305-764, Korea","institution_ids":["https://openalex.org/I139264467","https://openalex.org/I196345858"]},{"raw_affiliation_string":"Chungnam National University, Daejeon, South Korea","institution_ids":["https://openalex.org/I196345858"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5063186029","display_name":"Kap\u2010Ho Lee","orcid":"https://orcid.org/0000-0001-6932-7210","observed_orcids":["https://orcid.org/0000-0001-6932-7210"]},"institutions":[{"id":"https://openalex.org/I139264467","display_name":"Seoul National University","ror":"https://ror.org/04h9pn542","country_code":"KR","type":"education","lineage":["https://openalex.org/I139264467"]},{"id":"https://openalex.org/I196345858","display_name":"Chungnam National University","ror":"https://ror.org/0227as991","country_code":"KR","type":"education","lineage":["https://openalex.org/I196345858"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"K.-H. Lee","raw_affiliation_strings":["Interdisciplinary Program for Biochemical Engineering and Biotechnology, College of Engineering, Seoul National University, Seoul 151-742, Korea, 2 School of Chemical and Biological Engineering, College of Engineering, Seoul National University, Seoul 151-742, Korea and 3 Department of Fine Chemical Engineering and Chemistry, Chungnam National University, Daejeon 305-764, Korea","Chungnam National University, Daejeon, South Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Interdisciplinary Program for Biochemical Engineering and Biotechnology, College of Engineering, Seoul National University, Seoul 151-742, Korea, 2 School of Chemical and Biological Engineering, College of Engineering, Seoul National University, Seoul 151-742, Korea and 3 Department of Fine Chemical Engineering and Chemistry, Chungnam National University, Daejeon 305-764, Korea","institution_ids":["https://openalex.org/I139264467","https://openalex.org/I196345858"]},{"raw_affiliation_string":"Chungnam National University, Daejeon, South Korea","institution_ids":["https://openalex.org/I196345858"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111775405","display_name":"Cha\u2010Yong Choi","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I139264467","display_name":"Seoul National University","ror":"https://ror.org/04h9pn542","country_code":"KR","type":"education","lineage":["https://openalex.org/I139264467"]},{"id":"https://openalex.org/I196345858","display_name":"Chungnam National University","ror":"https://ror.org/0227as991","country_code":"KR","type":"education","lineage":["https://openalex.org/I196345858"]}],"countries":["KR"],"is_corresponding":false,"raw_author_name":"C.-Y. Choi","raw_affiliation_strings":["Interdisciplinary Program for Biochemical Engineering and Biotechnology, College of Engineering, Seoul National University, Seoul 151-742, Korea, 2 School of Chemical and Biological Engineering, College of Engineering, Seoul National University, Seoul 151-742, Korea and 3 Department of Fine Chemical Engineering and Chemistry, Chungnam National University, Daejeon 305-764, Korea","Chungnam National University, Daejeon, South Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Interdisciplinary Program for Biochemical Engineering and Biotechnology, College of Engineering, Seoul National University, Seoul 151-742, Korea, 2 School of Chemical and Biological Engineering, College of Engineering, Seoul National University, Seoul 151-742, Korea and 3 Department of Fine Chemical Engineering and Chemistry, Chungnam National University, Daejeon 305-764, Korea","institution_ids":["https://openalex.org/I139264467","https://openalex.org/I196345858"]},{"raw_affiliation_string":"Chungnam National University, Daejeon, South Korea","institution_ids":["https://openalex.org/I196345858"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5051365211","display_name":"Dong\u2010Myung Kim","orcid":"https://orcid.org/0000-0001-7875-0694","observed_orcids":["https://orcid.org/0000-0001-7875-0694"]},"institutions":[{"id":"https://openalex.org/I139264467","display_name":"Seoul National University","ror":"https://ror.org/04h9pn542","country_code":"KR","type":"education","lineage":["https://openalex.org/I139264467"]},{"id":"https://openalex.org/I196345858","display_name":"Chungnam National University","ror":"https://ror.org/0227as991","country_code":"KR","type":"education","lineage":["https://openalex.org/I196345858"]}],"countries":["KR"],"is_corresponding":true,"raw_author_name":"D.-M. Kim","raw_affiliation_strings":["Interdisciplinary Program for Biochemical Engineering and Biotechnology, College of Engineering, Seoul National University, Seoul 151-742, Korea, 2 School of Chemical and Biological Engineering, College of Engineering, Seoul National University, Seoul 151-742, Korea and 3 Department of Fine Chemical Engineering and Chemistry, Chungnam National University, Daejeon 305-764, Korea","Chungnam National University, Daejeon, South Korea"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Interdisciplinary Program for Biochemical Engineering and Biotechnology, College of Engineering, Seoul National University, Seoul 151-742, Korea, 2 School of Chemical and Biological Engineering, College of Engineering, Seoul National University, Seoul 151-742, Korea and 3 Department of Fine Chemical Engineering and Chemistry, Chungnam National University, Daejeon 305-764, Korea","institution_ids":["https://openalex.org/I139264467","https://openalex.org/I196345858"]},{"raw_affiliation_string":"Chungnam National University, Daejeon, South Korea","institution_ids":["https://openalex.org/I196345858"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5051365211"],"corresponding_institution_ids":["https://openalex.org/I139264467","https://openalex.org/I196345858"],"apc_list":{"value":3992,"currency":"USD","value_usd":3992},"apc_paid":{"value":3992,"currency":"USD","value_usd":3992},"fwci":0.5038,"has_fulltext":true,"cited_by_count":37,"citation_normalized_percentile":{"value":0.59724489,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"35","issue":"4","first_page":"e21","last_page":"e21"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.9789999723434448,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.9789999723434448,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11970","display_name":"Molecular Biology Techniques and Applications","score":0.004399999976158142,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.0026000000070780516,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/signal-peptide","display_name":"signal peptide","score":0.9010000228881836},{"id":"https://openalex.org/keywords/cell-free-protein-synthesis","display_name":"cell-free protein synthesis","score":0.9200000166893005},{"id":"https://openalex.org/keywords/recombinant-protein-expression","display_name":"recombinant protein expression","score":0.843999981880188},{"id":"https://openalex.org/keywords/signal-peptide-cleavage","display_name":"signal peptide cleavage","score":0.5809999704360962},{"id":"https://openalex.org/keywords/signal-peptidase","display_name":"signal peptidase","score":0.9279999732971191},{"id":"https://openalex.org/keywords/triton-x-100","display_name":"Triton X-100","score":0.8529999852180481},{"id":"https://openalex.org/keywords/in-situ-cleavage","display_name":"in situ cleavage","score":0.4950000047683716}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8429019451141357},{"id":"https://openalex.org/C10858879","wikidata":"https://www.wikidata.org/wiki/Q899790","display_name":"Signal peptide","level":4,"score":0.7647297382354736},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.7503416538238525},{"id":"https://openalex.org/C2778665413","wikidata":"https://www.wikidata.org/wiki/Q189030","display_name":"Cell-free protein synthesis","level":3,"score":0.6183567047119141},{"id":"https://openalex.org/C3675279","wikidata":"https://www.wikidata.org/wiki/Q211935","display_name":"Protein biosynthesis","level":2,"score":0.5548497438430786},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5220470428466797},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.49595293402671814},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.4845239520072937},{"id":"https://openalex.org/C552990157","wikidata":"https://www.wikidata.org/wiki/Q7430","display_name":"DNA","level":2,"score":0.4797183871269226},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.4435274600982666},{"id":"https://openalex.org/C176990463","wikidata":"https://www.wikidata.org/wiki/Q1854186","display_name":"Start codon","level":4,"score":0.4416118562221527},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.42980071902275085},{"id":"https://openalex.org/C105580179","wikidata":"https://www.wikidata.org/wiki/Q188928","display_name":"Messenger RNA","level":3,"score":0.3913441598415375}],"mesh":[{"descriptor_ui":"D002474","descriptor_name":"Cell-Free System","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003062","descriptor_name":"Codon","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011993","descriptor_name":"Recombinant Fusion Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014176","descriptor_name":"Protein Biosynthesis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D015202","descriptor_name":"Protein Engineering","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D016133","descriptor_name":"Polymerase Chain Reaction","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016366","descriptor_name":"Open Reading Frames","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D021382","descriptor_name":"Protein Sorting Signals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1093/nar/gkl917","doi":"https://doi.org/10.1093/nar/gkl917","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/gkl917","pdf_url":"https://academic.oup.com/nar/article-pdf/35/4/e21/4916234/gkl917.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:450336","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC1849898","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"other-oa","license_id":"https://openalex.org/licenses/other-oa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:1849898","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1849898","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Res","raw_type":"Text"},{"id":"pmid:17185295","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/17185295","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic acids research","raw_type":"Evaluation Study"}],"best_oa_location":{"id":"doi:10.1093/nar/gkl917","doi":"https://doi.org/10.1093/nar/gkl917","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/gkl917","pdf_url":"https://academic.oup.com/nar/article-pdf/35/4/e21/4916234/gkl917.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":"cc-by-nc","license_id":"https://openalex.org/licenses/cc-by-nc","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2124366355.pdf","grobid_xml":"https://content.openalex.org/works/W2124366355.grobid-xml"},"referenced_works_count":32,"referenced_works":["https://openalex.org/W1602915164","https://openalex.org/W1974027032","https://openalex.org/W1975085160","https://openalex.org/W1978110695","https://openalex.org/W1982214572","https://openalex.org/W1992226159","https://openalex.org/W2003546739","https://openalex.org/W2006243507","https://openalex.org/W2016629242","https://openalex.org/W2018147562","https://openalex.org/W2031473433","https://openalex.org/W2031853427","https://openalex.org/W2033570162","https://openalex.org/W2041166318","https://openalex.org/W2043411044","https://openalex.org/W2059138111","https://openalex.org/W2062276426","https://openalex.org/W2064907295","https://openalex.org/W2070818350","https://openalex.org/W2083871052","https://openalex.org/W2086425477","https://openalex.org/W2089165095","https://openalex.org/W2101918141","https://openalex.org/W2102751794","https://openalex.org/W2110423740","https://openalex.org/W2111929876","https://openalex.org/W2121553505","https://openalex.org/W2124342659","https://openalex.org/W2131303710","https://openalex.org/W2165352553","https://openalex.org/W2165375830","https://openalex.org/W2170602689"],"related_works":["https://openalex.org/W1979601841","https://openalex.org/W2046427631","https://openalex.org/W1967135643","https://openalex.org/W2739896841","https://openalex.org/W2015943850","https://openalex.org/W2414561315","https://openalex.org/W2405199870","https://openalex.org/W2027791722","https://openalex.org/W2031807129","https://openalex.org/W4280615409"],"abstract_inverted_index":{"This":[0],"study":[1],"developed":[2],"a":[3,13],"method":[4],"to":[5,41],"boost":[6],"the":[7,28,32,51,62,65,69,73,76,88,103,115,120,128,134,140,144,147],"expression":[8],"of":[9,31,50,56,64,68,75,90,122,130,139,146,157],"recombinant":[10,158],"proteins":[11,105,159],"in":[12,87,99,108,119,133],"cell-free":[14,116],"protein":[15,43,78],"synthesis":[16,44,117],"system":[17],"without":[18],"leaving":[19],"additional":[20],"amino":[21],"acid":[22],"residues.":[23],"It":[24],"was":[25,58,79],"found":[26],"that":[27],"nucleotide":[29],"sequences":[30,149],"signal":[33,70,96,131,148],"peptides":[34,97],"serve":[35],"as":[36,82,84],"an":[37,91],"efficient":[38],"downstream":[39],"box":[40],"stimulate":[42],"when":[45],"they":[46],"were":[47,98,106,150,160],"fused":[48],"upstream":[49],"target":[52,104],"genes.":[53],"The":[54,95,137],"extent":[55],"stimulation":[57],"critically":[59],"affected":[60],"by":[61,81,112,152],"identity":[63],"second":[66,93],"codons":[67],"sequences.":[71],"Moreover,":[72],"yield":[74],"synthesized":[77],"enhanced":[80],"much":[83],"10":[85],"times":[86],"presence":[89,121],"optimal":[92],"codon.":[94],"situ":[100],"cleaved":[101],"and":[102,143],"produced":[107],"their":[109],"native":[110],"sizes":[111],"carrying":[113],"out":[114],"reactions":[118],"Triton":[123],"X-100,":[124],"most":[125],"likely":[126],"through":[127],"activation":[129],"peptidase":[132],"S30":[135],"extract.":[136],"amplification":[138],"template":[141],"DNA":[142],"addition":[145],"accomplished":[151],"PCR.":[153],"Hence,":[154],"elevated":[155],"levels":[156],"generated":[161],"within":[162],"several":[163],"hours.":[164]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":4},{"year":2017,"cited_by_count":3},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":5},{"year":2012,"cited_by_count":4}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}