{"id":"https://openalex.org/W2122335902","doi":null,"title":"Signalling pathways involved in insulin cardioprotection : are they comparable in normoxic perfused isolated rat heart vs. ischaemia/reperfusion model?","display_name":"Signalling pathways involved in insulin cardioprotection : are they comparable in normoxic perfused isolated rat heart vs. ischaemia/reperfusion model?","publication_year":2006,"publication_date":"2006-12-01","ids":{"openalex":"https://openalex.org/W2122335902","mag":"2122335902"},"language":"en","primary_location":{"id":"pmh:oai:scholar.sun.ac.za:10019.1/17380","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/10019.1/17380","pdf_url":null,"source":{"id":"https://openalex.org/S4306400300","display_name":"SUNScholar (Stellenbosch University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I26092322","host_organization_name":"Stellenbosch University","host_organization_lineage":["https://openalex.org/I26092322"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5066156894","display_name":"Edith Sylvie Manga-Manguiya","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":true,"raw_author_name":"Edith Sylvie Manga-Manguiya","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":["https://openalex.org/A5066156894"],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11057","display_name":"Cardiac Ischemia and Reperfusion","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/2705","display_name":"Cardiology and Cardiovascular Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T11057","display_name":"Cardiac Ischemia and Reperfusion","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/2705","display_name":"Cardiology and Cardiovascular Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10821","display_name":"Cardiovascular Function and Risk Factors","score":0.000699999975040555,"subfield":{"id":"https://openalex.org/subfields/2705","display_name":"Cardiology and Cardiovascular Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10839","display_name":"Pancreatic function and diabetes","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2712","display_name":"Endocrinology, Diabetes and Metabolism"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/normoxic-conditions","display_name":"normoxic conditions","score":0.5809999704360962},{"id":"https://openalex.org/keywords/positive-inotropic-effect","display_name":"positive inotropic effect","score":0.7229999899864197},{"id":"https://openalex.org/keywords/akt","display_name":"Akt","score":0.9070000052452087},{"id":"https://openalex.org/keywords/wortmannin","display_name":"wortmannin","score":0.7570000290870667},{"id":"https://openalex.org/keywords/l-name","display_name":"L-NAME","score":0.9380000233650208},{"id":"https://openalex.org/keywords/nitric-oxide-synthase","display_name":"nitric oxide synthase","score":0.9079999923706055},{"id":"https://openalex.org/keywords/coronary-blood-flow","display_name":"coronary blood flow","score":0.8579999804496765},{"id":"https://openalex.org/keywords/left-ventricular-contractility","display_name":"left ventricular contractility","score":0.7770000100135803},{"id":"https://openalex.org/keywords/isolated-perfused-rat-heart","display_name":"isolated perfused rat heart","score":0.7310000061988831},{"id":"https://openalex.org/keywords/ischemia-reperfusion-injury","display_name":"ischemia-reperfusion injury","score":0.8299999833106995},{"id":"https://openalex.org/keywords/insulin-dose-response","display_name":"insulin dose-response","score":0.5059999823570251},{"id":"https://openalex.org/keywords/p70s6k","display_name":"p70S6K","score":0.6899999976158142},{"id":"https://openalex.org/keywords/erk","display_name":"ERK","score":0.7210000157356262}],"concepts":[{"id":"https://openalex.org/C2779676291","wikidata":"https://www.wikidata.org/wiki/Q25325349","display_name":"Cardioprotection","level":3,"score":0.9412864446640015},{"id":"https://openalex.org/C541997718","wikidata":"https://www.wikidata.org/wiki/Q188151","display_name":"Ischemia","level":2,"score":0.515381932258606},{"id":"https://openalex.org/C3018449070","wikidata":"https://www.wikidata.org/wiki/Q828130","display_name":"Signalling pathways","level":3,"score":0.4762313961982727},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.47555726766586304},{"id":"https://openalex.org/C164705383","wikidata":"https://www.wikidata.org/wiki/Q10379","display_name":"Cardiology","level":1,"score":0.45726972818374634},{"id":"https://openalex.org/C2779306644","wikidata":"https://www.wikidata.org/wiki/Q2002370","display_name":"Insulin","level":2,"score":0.4528709948062897},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.4274861216545105},{"id":"https://openalex.org/C3020526226","wikidata":"https://www.wikidata.org/wiki/Q844935","display_name":"Myocardial ischaemia","level":3,"score":0.4192652404308319},{"id":"https://openalex.org/C98274493","wikidata":"https://www.wikidata.org/wiki/Q128406","display_name":"Pharmacology","level":1,"score":0.3878211975097656},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.11122673749923706}],"mesh":[],"locations_count":2,"locations":[{"id":"pmh:oai:scholar.sun.ac.za:10019.1/17380","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/10019.1/17380","pdf_url":null,"source":{"id":"https://openalex.org/S4306400300","display_name":"SUNScholar (Stellenbosch University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I26092322","host_organization_name":"Stellenbosch University","host_organization_lineage":["https://openalex.org/I26092322"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},{"id":"mag:2122335902","doi":null,"is_oa":false,"landing_page_url":"http://scholar.sun.ac.za/bitstream/10019.1/17380/1/Manga-Manguiya_signalling_2006.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.9476000070571899,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":179,"referenced_works":["https://openalex.org/W67399661","https://openalex.org/W81782688","https://openalex.org/W89084389","https://openalex.org/W202438774","https://openalex.org/W303812557","https://openalex.org/W1531537331","https://openalex.org/W1546773941","https://openalex.org/W1558755669","https://openalex.org/W1569626229","https://openalex.org/W1572009433","https://openalex.org/W1581310431","https://openalex.org/W1649021695","https://openalex.org/W1726025741","https://openalex.org/W1776121512","https://openalex.org/W1781422850","https://openalex.org/W1782741946","https://openalex.org/W1791888878","https://openalex.org/W1818313483","https://openalex.org/W1829658772","https://openalex.org/W1833083308","https://openalex.org/W1842310408","https://openalex.org/W1862594138","https://openalex.org/W1946614535","https://openalex.org/W1970118794","https://openalex.org/W1972040145","https://openalex.org/W1973375324","https://openalex.org/W1977328014","https://openalex.org/W1977590844","https://openalex.org/W1979292368","https://openalex.org/W1979381792","https://openalex.org/W1980897193","https://openalex.org/W1981150853","https://openalex.org/W1983051517","https://openalex.org/W1984401650","https://openalex.org/W1985113364","https://openalex.org/W1987296421","https://openalex.org/W1988068201","https://openalex.org/W1989294105","https://openalex.org/W1989705400","https://openalex.org/W1993775690","https://openalex.org/W1994253356","https://openalex.org/W1996163407","https://openalex.org/W1997862023","https://openalex.org/W1997954665","https://openalex.org/W2000549928","https://openalex.org/W2004078164","https://openalex.org/W2004134377","https://openalex.org/W2005214072","https://openalex.org/W2006933647","https://openalex.org/W2007472328","https://openalex.org/W2007731253","https://openalex.org/W2008987764","https://openalex.org/W2010461890","https://openalex.org/W2012083843","https://openalex.org/W2012217185","https://openalex.org/W2013293486","https://openalex.org/W2013874802","https://openalex.org/W2017221193","https://openalex.org/W2017641447","https://openalex.org/W2019872821","https://openalex.org/W2020931266","https://openalex.org/W2022131685","https://openalex.org/W2022271696","https://openalex.org/W2022822102","https://openalex.org/W2024306318","https://openalex.org/W2024556437","https://openalex.org/W2024862570","https://openalex.org/W2025654952","https://openalex.org/W2026025408","https://openalex.org/W2026145775","https://openalex.org/W2026178023","https://openalex.org/W2027702390","https://openalex.org/W2028923300","https://openalex.org/W2029765627","https://openalex.org/W2030573603","https://openalex.org/W2030604715","https://openalex.org/W2031504013","https://openalex.org/W2033801757","https://openalex.org/W2034493528","https://openalex.org/W2034597681","https://openalex.org/W2038594983","https://openalex.org/W2039456527","https://openalex.org/W2041693042","https://openalex.org/W2044391218","https://openalex.org/W2044438496","https://openalex.org/W2044693162","https://openalex.org/W2044868069","https://openalex.org/W2045278002","https://openalex.org/W2045658220","https://openalex.org/W2046867705","https://openalex.org/W2048121649","https://openalex.org/W2048782251","https://openalex.org/W2051031104","https://openalex.org/W2051034327","https://openalex.org/W2053609044","https://openalex.org/W2053773941","https://openalex.org/W2056761641","https://openalex.org/W2057168930","https://openalex.org/W2061245367","https://openalex.org/W2061914106","https://openalex.org/W2064727002","https://openalex.org/W2064801081","https://openalex.org/W2065547075","https://openalex.org/W2066457776","https://openalex.org/W2070094431","https://openalex.org/W2070732456","https://openalex.org/W2071323212","https://openalex.org/W2072509458","https://openalex.org/W2074478235","https://openalex.org/W2075068003","https://openalex.org/W2075360440","https://openalex.org/W2076035242","https://openalex.org/W2076186944","https://openalex.org/W2079097756","https://openalex.org/W2080334804","https://openalex.org/W2080791510","https://openalex.org/W2085456568","https://openalex.org/W2086120385","https://openalex.org/W2090526906","https://openalex.org/W2094038408","https://openalex.org/W2094079572","https://openalex.org/W2095488878","https://openalex.org/W2095707818","https://openalex.org/W2097767970","https://openalex.org/W2100143490","https://openalex.org/W2101513893","https://openalex.org/W2101744962","https://openalex.org/W2108878535","https://openalex.org/W2112576454","https://openalex.org/W2112620000","https://openalex.org/W2113657795","https://openalex.org/W2119693754","https://openalex.org/W2120315787","https://openalex.org/W2120339303","https://openalex.org/W2121786098","https://openalex.org/W2123890069","https://openalex.org/W2127888338","https://openalex.org/W2128635872","https://openalex.org/W2129329622","https://openalex.org/W2132136466","https://openalex.org/W2132795937","https://openalex.org/W2135002576","https://openalex.org/W2136902776","https://openalex.org/W2147360618","https://openalex.org/W2147831550","https://openalex.org/W2148099496","https://openalex.org/W2148573933","https://openalex.org/W2150684620","https://openalex.org/W2150739709","https://openalex.org/W2152744853","https://openalex.org/W2153491246","https://openalex.org/W2154713680","https://openalex.org/W2155034650","https://openalex.org/W2155668811","https://openalex.org/W2156802607","https://openalex.org/W2158234045","https://openalex.org/W2158639771","https://openalex.org/W2158990159","https://openalex.org/W2159505514","https://openalex.org/W2161444026","https://openalex.org/W2163440329","https://openalex.org/W2163546545","https://openalex.org/W2164139183","https://openalex.org/W2164323243","https://openalex.org/W2164471793","https://openalex.org/W2165604272","https://openalex.org/W2168085443","https://openalex.org/W2168612295","https://openalex.org/W2171908036","https://openalex.org/W2221423833","https://openalex.org/W2270060950","https://openalex.org/W2270264925","https://openalex.org/W2338467026","https://openalex.org/W2442275722","https://openalex.org/W2761559457","https://openalex.org/W2911242374","https://openalex.org/W4231383319","https://openalex.org/W4292820908","https://openalex.org/W4322388182"],"related_works":["https://openalex.org/W2372489660","https://openalex.org/W2170956838","https://openalex.org/W4282564885","https://openalex.org/W2038836792","https://openalex.org/W2065599876","https://openalex.org/W2012217185","https://openalex.org/W2069031285","https://openalex.org/W2017221193","https://openalex.org/W7041980713","https://openalex.org/W2076754919"],"abstract_inverted_index":{"ENGLISH":[0],"ABSTRACT:":[1],"Introduction:":[2],"It":[3,31],"is":[4],"well":[5,276,455],"documented":[6],"that":[7,35,81,109,306,323,335,363,372,408,429],"insulin":[8,47,69,82,116,144,192,246,249,289,325,337,355,376,420,441,471],"offers":[9],"cardioprotection":[10,44],"against":[11],"the":[12,43,66,71,89,113,132,159,172,175,191,210,252,259,302,317,321,343,361,392,416,437,446,459],"consequences":[13],"of":[14,27,46,68,115,134,143,184,216,288,375,396,413,419,440,463,470],"ischaemia/reperfusion":[15],"injury.":[16],"Insulin-induced":[17],"improvements":[18],"in":[19,25,42,104,163,166,282,301,313,333,352,384,403,406,436,445],"cardiac":[20,118,195,296,382,422,443],"functions":[21,196],"are":[22],"widely":[23],"investigated":[24,101],"models":[26],"ischaemia":[28],"and":[29,58,120,174,178,186,201,271,280,320,339,411,475,480],"reperfusion.":[30],"has":[32,78,83,99],"been":[33,79,100],"shown":[34],"many":[36,59],"signalling":[37],"pathways":[38,52,121],"may":[39,126,427,432],"be":[40,152,350],"involved":[41,122,162],"properties":[45],"under":[48,73,123,154,197,450],"those":[49],"conditions.":[50,199,452],"These":[51],"include":[53],"PI3-K,":[54],"PKB/Akt,":[55],"p70S6k,":[56],"ERK":[57],"others.":[60],"However,":[61],"little":[62],"data":[63],"exists":[64],"on":[65,70,88,117,190,194,381,421,442,477],"effects":[67,114,193,380,418,462,469],"heart":[72,268,283,448,478],"normoxic":[74,90,124,155,198,385,451],"condition.":[75],"Some":[76],"evidence":[77],"presented":[80],"a":[84,140,147,181,310,401,434],"positive":[85,148,378,438,460],"inotropic":[86,149,379,461],"effect":[87,439],"perfused":[91,207,220,386,449],"rat":[92,204,387],"heart,":[93],"but":[94],"no":[95],"precise":[96],"cellular":[97],"mechanism":[98],"or":[102,240,248,258,366],"described":[103],"this":[105],"regard.":[106],"We":[107],"believe":[108],"an":[110],"investigation":[111],"into":[112],"function":[119,297,314,383,444],"conditions":[125],"help":[127],"us":[128],"to":[129,157,171,179,299,316,342,349,360],"better":[130],"understand":[131],"mechanisms":[133,161],"insulin-induced":[135,164],"cardioprotection.":[136],"Aims:":[137],"To":[138],"determine":[139],"suitable":[141],"dose":[142],"at":[145,291],"which":[146],"response":[150],"could":[151],"detectable":[153],"conditions,":[156],"investigate":[158,180],"possible":[160,182],"increases":[165],"contractility":[167],"with":[168,225,235,241,251,391],"specific":[169],"reference":[170],"vasculature":[173],"coronary":[176,272,481],"flow":[177,273],"involvement":[183],"PI3-K":[185,260,279,347,397,476],"its":[187],"downstream":[188],"effectors":[189],"Materials":[200],"methods:":[202],"Isolated":[203],"hearts":[205,218,300,319,322,334,353,362,388,407],"were":[206,219,284],"retrogradely":[208],"using":[209],"Langendorff":[211],"technique.":[212],"After":[213],"10":[214],"minutes":[215,223],"stabilization":[217],"for":[221],"30":[222],"either":[224],"standard":[226,242],"perfusion":[227,243],"solution":[228,244],"i.e.":[229],"Krebs-Henseleit":[230],"buffer":[231],"+":[232],"glucose":[233],"gassed":[234],"95%O2,":[236],"5%CO2":[237],"(control":[238],"hearts),":[239],"plus":[245],"alone":[247,290,326,338,358],"together":[250],"nitric":[253,430,473],"oxide":[254,431,474],"synthase":[255],"inhibitor":[256,261],"L-NAME":[257,414,453],"wortmannin.":[262],"Left":[263],"ventricular":[264],"developed":[265],"pressure":[266],"(LVDevP),":[267],"rate":[269,479],"(HR)":[270],"(CF)":[274],"as":[275,277,389,454,456],"phosphorylated":[278,404],"PKB/Akt":[281,329,405],"measured.":[285],"Results:":[286],"Administration":[287],"physiological":[292,373],"concentrations":[293,374],"showed":[294,309],"improved":[295,393],"compared":[298,315,341,359],"control":[303,318,344],"group.":[304],"Hearts":[305],"received":[307,324,336,364,409],"insulin+L-NAME":[308,340,365],"significant":[311],"decrease":[312,402],"(p<0.05).":[327],"Phosphorylated":[328,346],"(Thr308)":[330],"was":[331,356],"increased":[332],"hearts.":[345],"tended":[348],"higher":[351],"where":[354],"administered":[357],"insulin+wortmannin.":[367],"Conclusion:":[368],"This":[369],"study":[370],"confirmed":[371],"exert":[377],"seen":[390],"LVDevP.":[394],"Inhibition":[395],"by":[398],"wortmannin":[399,457],"induced":[400],"insulin+wortmannin":[410],"administration":[412],"impaired":[415],"beneficial":[417],"functions.":[423],"Therefore":[424],"these":[425],"results":[426],"indicate":[428],"have":[433],"role":[435],"healthy":[447],"reversed":[458],"insulin.":[464],"Both":[465],"inhibitors":[466],"also":[467],"unmasked":[468],"via":[472],"flow.":[482]},"counts_by_year":[],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}