{"id":"https://openalex.org/W2121998297","doi":"https://doi.org/10.7554/elife.09771","title":"Rapid cell-free forward engineering of novel genetic ring oscillators","display_name":"Rapid cell-free forward engineering of novel genetic ring oscillators","publication_year":2015,"publication_date":"2015-10-02","ids":{"openalex":"https://openalex.org/W2121998297","doi":"https://doi.org/10.7554/elife.09771","mag":"2121998297","pmid":"https://pubmed.ncbi.nlm.nih.gov/26430766","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/4714972"},"language":"en","primary_location":{"id":"doi:10.7554/elife.09771","doi":"https://doi.org/10.7554/elife.09771","is_oa":true,"landing_page_url":"https://doi.org/10.7554/elife.09771","pdf_url":null,"source":{"id":"https://openalex.org/S1336409049","display_name":"eLife","issn_l":"2050-084X","issn":["2050-084X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","jufo-2","ki-jl-2","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311710","host_organization_name":"eLife Sciences Publications Ltd","host_organization_lineage":["https://openalex.org/P4310311710"],"host_organization_lineage_names":["eLife Sciences Publications Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"eLife","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.7554/elife.09771","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5039101195","display_name":"Henrike Niederholtmeyer","orcid":"https://orcid.org/0000-0002-1375-0287","observed_orcids":["https://orcid.org/0000-0002-1375-0287"]},"institutions":[{"id":"https://openalex.org/I5124864","display_name":"\u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne","ror":"https://ror.org/02s376052","country_code":"CH","type":"education","lineage":["https://openalex.org/I2799323385","https://openalex.org/I5124864"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Henrike Niederholtmeyer","raw_affiliation_strings":["Institute of Bioengineering, School of Engineering, \u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne, Lausanne, Switzerland","Institute of Bioengineering, School of Engineering, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Bioengineering, School of Engineering, \u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne, Lausanne, Switzerland","institution_ids":["https://openalex.org/I5124864"]},{"raw_affiliation_string":"Institute of Bioengineering, School of Engineering, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland","institution_ids":["https://openalex.org/I5124864"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5089078059","display_name":"Zachary Z. Sun","orcid":"https://orcid.org/0000-0002-9425-2924","observed_orcids":["https://orcid.org/0000-0002-9425-2924"]},"institutions":[{"id":"https://openalex.org/I122411786","display_name":"California Institute of Technology","ror":"https://ror.org/05dxps055","country_code":"US","type":"education","lineage":["https://openalex.org/I122411786"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Zachary Z. Sun","raw_affiliation_strings":["Division of Biology and Bioengineering, California Institute of Technology, Pasadena, CA, USA","Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Biology and Bioengineering, California Institute of Technology, Pasadena, CA, USA","institution_ids":["https://openalex.org/I122411786"]},{"raw_affiliation_string":"Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, United States","institution_ids":["https://openalex.org/I122411786"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5012594690","display_name":"Yutaka Hori","orcid":"https://orcid.org/0000-0002-3253-4985","observed_orcids":["https://orcid.org/0000-0002-3253-4985"]},"institutions":[{"id":"https://openalex.org/I122411786","display_name":"California Institute of Technology","ror":"https://ror.org/05dxps055","country_code":"US","type":"education","lineage":["https://openalex.org/I122411786"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Yutaka Hori","raw_affiliation_strings":["Department of Control and Dynamical Systems, California Institute of Technology","Division of Engineering and Applied Science, California Institute of Technology, Pasadena, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Control and Dynamical Systems, California Institute of Technology","institution_ids":["https://openalex.org/I122411786"]},{"raw_affiliation_string":"Division of Engineering and Applied Science, California Institute of Technology, Pasadena, United States","institution_ids":["https://openalex.org/I122411786"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5091530669","display_name":"Enoch Yeung","orcid":"https://orcid.org/0000-0001-7630-7429","observed_orcids":["https://orcid.org/0000-0001-7630-7429"]},"institutions":[{"id":"https://openalex.org/I122411786","display_name":"California Institute of Technology","ror":"https://ror.org/05dxps055","country_code":"US","type":"education","lineage":["https://openalex.org/I122411786"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Enoch Yeung","raw_affiliation_strings":["Department of Control and Dynamical Systems, California Institute of Technology","Division of Engineering and Applied Science, California Institute of Technology, Pasadena, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Control and Dynamical Systems, California Institute of Technology","institution_ids":["https://openalex.org/I122411786"]},{"raw_affiliation_string":"Division of Engineering and Applied Science, California Institute of Technology, Pasadena, United States","institution_ids":["https://openalex.org/I122411786"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5077479614","display_name":"Amanda Verpoorte","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I5124864","display_name":"\u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne","ror":"https://ror.org/02s376052","country_code":"CH","type":"education","lineage":["https://openalex.org/I2799323385","https://openalex.org/I5124864"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Amanda Verpoorte","raw_affiliation_strings":["Institute of Bioengineering, School of Engineering, \u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne, Lausanne, Switzerland","Institute of Bioengineering, School of Engineering, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Bioengineering, School of Engineering, \u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne, Lausanne, Switzerland","institution_ids":["https://openalex.org/I5124864"]},{"raw_affiliation_string":"Institute of Bioengineering, School of Engineering, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland","institution_ids":["https://openalex.org/I5124864"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068600217","display_name":"Richard M. Murray","orcid":"https://orcid.org/0000-0002-5785-7481","observed_orcids":["https://orcid.org/0000-0002-5785-7481"]},"institutions":[{"id":"https://openalex.org/I122411786","display_name":"California Institute of Technology","ror":"https://ror.org/05dxps055","country_code":"US","type":"education","lineage":["https://openalex.org/I122411786"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Richard M. Murray","raw_affiliation_strings":["Department of Control and Dynamical Systems, California Institute of Technology","Division of Biology and Bioengineering, California Institute of Technology, Pasadena, CA, USA","Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, United States","Division of Engineering and Applied Science, California Institute of Technology, Pasadena, United States"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Control and Dynamical Systems, California Institute of Technology","institution_ids":["https://openalex.org/I122411786"]},{"raw_affiliation_string":"Division of Biology and Bioengineering, California Institute of Technology, Pasadena, CA, USA","institution_ids":["https://openalex.org/I122411786"]},{"raw_affiliation_string":"Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, United States","institution_ids":["https://openalex.org/I122411786"]},{"raw_affiliation_string":"Division of Engineering and Applied Science, California Institute of Technology, Pasadena, United States","institution_ids":["https://openalex.org/I122411786"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5087352392","display_name":"Sebastian J. Maerkl","orcid":"https://orcid.org/0000-0003-1917-5268","observed_orcids":["https://orcid.org/0000-0003-1917-5268"]},"institutions":[{"id":"https://openalex.org/I5124864","display_name":"\u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne","ror":"https://ror.org/02s376052","country_code":"CH","type":"education","lineage":["https://openalex.org/I2799323385","https://openalex.org/I5124864"]}],"countries":["CH"],"is_corresponding":false,"raw_author_name":"Sebastian J. Maerkl","raw_affiliation_strings":["Institute of Bioengineering, School of Engineering, \u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne, Lausanne, Switzerland","Institute of Bioengineering, School of Engineering, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Bioengineering, School of Engineering, \u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne, Lausanne, Switzerland","institution_ids":["https://openalex.org/I5124864"]},{"raw_affiliation_string":"Institute of Bioengineering, School of Engineering, Ecole Polytechnique Federale de Lausanne, Lausanne, Switzerland","institution_ids":["https://openalex.org/I5124864"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":3000,"currency":"USD","value_usd":2500},"apc_paid":{"value":3000,"currency":"USD","value_usd":2500},"fwci":8.1914,"has_fulltext":false,"cited_by_count":233,"citation_normalized_percentile":{"value":0.98555931,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":97,"max":100},"biblio":{"volume":"4","issue":null,"first_page":"e09771","last_page":"e09771"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10621","display_name":"Gene Regulatory Network Analysis","score":0.9916999936103821,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10621","display_name":"Gene Regulatory Network Analysis","score":0.9916999936103821,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10932","display_name":"Microbial Metabolic Engineering and Bioproduction","score":0.003599999938160181,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.0008999999845400453,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cell-free-synthetic-biology","display_name":"cell-free synthetic biology","score":0.9380000233650208},{"id":"https://openalex.org/keywords/repressilator","display_name":"repressilator","score":0.9700000286102295},{"id":"https://openalex.org/keywords/synthetic-gene-networks","display_name":"synthetic gene networks","score":0.781000018119812},{"id":"https://openalex.org/keywords/circuit-characterization","display_name":"circuit characterization","score":0.44200000166893005},{"id":"https://openalex.org/keywords/in-vitro-to-in-vivo-transfer","display_name":"in vitro to in vivo transfer","score":0.7160000205039978},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.9480000138282776},{"id":"https://openalex.org/keywords/design-build-test-cycle","display_name":"design-build-test cycle","score":0.6389999985694885}],"concepts":[{"id":"https://openalex.org/C2780378348","wikidata":"https://www.wikidata.org/wiki/Q25351438","display_name":"Ring (chemistry)","level":2,"score":0.5665668249130249},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.39586517214775085},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.38308805227279663},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.37081217765808105},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.22974762320518494},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D002474","descriptor_name":"Cell-Free System","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008164","descriptor_name":"Luminescent Proteins","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D008164","descriptor_name":"Luminescent Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D017930","descriptor_name":"Genes, Reporter","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D053263","descriptor_name":"Gene Regulatory Networks","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D058615","descriptor_name":"Synthetic Biology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":7,"locations":[{"id":"doi:10.7554/elife.09771","doi":"https://doi.org/10.7554/elife.09771","is_oa":true,"landing_page_url":"https://doi.org/10.7554/elife.09771","pdf_url":null,"source":{"id":"https://openalex.org/S1336409049","display_name":"eLife","issn_l":"2050-084X","issn":["2050-084X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","jufo-2","ki-jl-2","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311710","host_organization_name":"eLife Sciences Publications Ltd","host_organization_lineage":["https://openalex.org/P4310311710"],"host_organization_lineage_names":["eLife Sciences Publications Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"eLife","raw_type":"journal-article"},{"id":"doi:10.1101/018317","doi":"https://doi.org/10.1101/018317","is_oa":true,"landing_page_url":"https://doi.org/10.1101/018317","pdf_url":"https://www.biorxiv.org/content/biorxiv/early/2015/04/21/018317.full.pdf","source":{"id":"https://openalex.org/S4306402567","display_name":"bioRxiv (Cold Spring Harbor Laboratory)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I2750212522","host_organization_name":"Cold Spring Harbor Laboratory","host_organization_lineage":["https://openalex.org/I2750212522"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"bioRxiv","raw_type":"posted-content"},{"id":"pmh:oai:pubmedcentral.nih.gov:4714972","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4714972","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"eLife","raw_type":"Text"},{"id":"pmid:26430766","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/26430766","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"eLife","raw_type":"Journal Article"},{"id":"pmh:oai:authors.library.caltech.edu:61028","doi":null,"is_oa":true,"landing_page_url":"https://resolver.caltech.edu/CaltechAUTHORS:20151013-090251954","pdf_url":null,"source":{"id":"https://openalex.org/S4306402161","display_name":"CaltechAUTHORS (California Institute of Technology)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I122411786","host_organization_name":"California Institute of Technology","host_organization_lineage":["https://openalex.org/I122411786"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Article"},{"id":"pmh:oai:doaj.org/article:312ccabb1100424e8de8154c7f0438ae","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/312ccabb1100424e8de8154c7f0438ae","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"eLife, Vol 4 (2015)","raw_type":"article"},{"id":"pmh:oai:infoscience.epfl.ch:271175","doi":null,"is_oa":true,"landing_page_url":"http://infoscience.epfl.ch/record/271175","pdf_url":null,"source":{"id":"https://openalex.org/S4306400487","display_name":"Infoscience (Ecole Polytechnique F\u00e9d\u00e9rale de Lausanne)","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I5124864","host_organization_name":"\u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne","host_organization_lineage":["https://openalex.org/I5124864"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-nd","license_id":"https://openalex.org/licenses/cc-by-nc-nd","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":"research article"}],"best_oa_location":{"id":"doi:10.7554/elife.09771","doi":"https://doi.org/10.7554/elife.09771","is_oa":true,"landing_page_url":"https://doi.org/10.7554/elife.09771","pdf_url":null,"source":{"id":"https://openalex.org/S1336409049","display_name":"eLife","issn_l":"2050-084X","issn":["2050-084X"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","jufo-2","ki-jl-2","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311710","host_organization_name":"eLife Sciences Publications Ltd","host_organization_lineage":["https://openalex.org/P4310311710"],"host_organization_lineage_names":["eLife Sciences Publications Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"eLife","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2515541506","display_name":null,"funder_award_id":"HR0011","funder_id":"https://openalex.org/F4320332180","funder_display_name":"Defense Advanced Research Projects Agency"},{"id":"https://openalex.org/G2993362680","display_name":null,"funder_award_id":"HR0011-12-C-0065","funder_id":"https://openalex.org/F4320332180","funder_display_name":"Defense Advanced Research Projects Agency"},{"id":"https://openalex.org/G8627071773","display_name":null,"funder_award_id":"MTO Living Foundries HR0011-12-C-0065","funder_id":"https://openalex.org/F4320332180","funder_display_name":"Defense Advanced Research Projects Agency"}],"funders":[{"id":"https://openalex.org/F4320309398","display_name":"California Institute of Technology","ror":"https://ror.org/05dxps055"},{"id":"https://openalex.org/F4320320915","display_name":"\u00c9cole Polytechnique F\u00e9d\u00e9rale de Lausanne","ror":"https://ror.org/02s376052"},{"id":"https://openalex.org/F4320332180","display_name":"Defense Advanced Research Projects Agency","ror":"https://ror.org/02caytj08"},{"id":"https://openalex.org/F4320332815","display_name":"Advanced Research Projects Agency","ror":"https://ror.org/02caytj08"},{"id":"https://openalex.org/F4320333566","display_name":"National Defense Science and Engineering Graduate","ror":null},{"id":"https://openalex.org/F4320334764","display_name":"Japan Society for the Promotion of Science","ror":"https://ror.org/00hhkn466"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2121998297.pdf","grobid_xml":"https://content.openalex.org/works/W2121998297.grobid-xml"},"referenced_works_count":39,"referenced_works":["https://openalex.org/W95237226","https://openalex.org/W1645305843","https://openalex.org/W1966645118","https://openalex.org/W1973256834","https://openalex.org/W1973577260","https://openalex.org/W1973873896","https://openalex.org/W1974028960","https://openalex.org/W1975379503","https://openalex.org/W1983913689","https://openalex.org/W1996666978","https://openalex.org/W2001426985","https://openalex.org/W2021022239","https://openalex.org/W2047453261","https://openalex.org/W2055605610","https://openalex.org/W2057993765","https://openalex.org/W2061094822","https://openalex.org/W2062162460","https://openalex.org/W2071463351","https://openalex.org/W2087993212","https://openalex.org/W2103053013","https://openalex.org/W2105057212","https://openalex.org/W2107403327","https://openalex.org/W2114228460","https://openalex.org/W2124013800","https://openalex.org/W2132724170","https://openalex.org/W2132828256","https://openalex.org/W2137772471","https://openalex.org/W2141564052","https://openalex.org/W2147775831","https://openalex.org/W2148641266","https://openalex.org/W2153067536","https://openalex.org/W2166572577","https://openalex.org/W2167279371","https://openalex.org/W2169779483","https://openalex.org/W2317579024","https://openalex.org/W2327010950","https://openalex.org/W2950851734","https://openalex.org/W4206621167","https://openalex.org/W4236366502"],"related_works":["https://openalex.org/W2917657657","https://openalex.org/W7096733761","https://openalex.org/W2946280884","https://openalex.org/W2107403327","https://openalex.org/W2624888310","https://openalex.org/W1974281125","https://openalex.org/W6983816276","https://openalex.org/W2177943983","https://openalex.org/W1963611162","https://openalex.org/W2989928339"],"abstract_inverted_index":{"SUMMARY:":[0],"While":[1],"complex":[2,93],"dynamic":[3,123],"biological":[4,79,116,255],"networks":[5,18,37,62,95,124],"control":[6],"gene":[7,61],"expression":[8],"and":[9,26,32,47,55,72,81,100,130,136,144,169,198,208,229,257,264],"metabolism":[10],"in":[11,57,63,98,108,132,155,206,213,225,232,238,254],"all":[12],"living":[13],"organisms,":[14],"engineering":[15,221,256],"comparable":[16],"synthetic":[17,36,94,122,242,263],"remains":[19,90],"challenging":[20],"1,2":[21],".":[22,87,157,240],"Conducting":[23],"extensive,":[24],"quantitative":[25,260],"rapid":[27,183],"characterization":[28,177,261],"during":[29],"the":[30,48,146,176,182,212,231,246,259],"design":[31],"implementation":[33],"process":[34],"of":[35,113,178,185,262],"is":[38,105],"currently":[39],"severely":[40],"limited":[41],"due":[42],"to":[43,68,75,111,115,139,164,181,201,248],"cumbersome":[44],"molecular":[45],"cloning":[46],"difficulties":[49],"associated":[50],"with":[51],"measuring":[52],"parts,":[53],"components":[54,180],"systems":[56,80],"cellular":[58,140],"hosts.":[59,141],"Engineering":[60],"a":[64,101,133,150,226],"cell-free":[65,102,134,162,191,227],"environment":[66],"promises":[67],"be":[69,112,126,223],"an":[70],"efficient":[71],"effective":[73],"approach":[74,192],"rapidly":[76],"develop":[77],"novel":[78,166,196],"understand":[82],"their":[83],"operating":[84],"regimes":[85],"3-5":[86],"However,":[88],"it":[89],"questionable":[91],"whether":[92],"behave":[96],"similarly":[97],"cells":[99],"environment,":[103],"which":[104],"critical":[106],"for":[107],"vitro":[109,156,214,233],"approaches":[110],"significance":[114],"engineering.":[117],"Here":[118],"we":[119],"show":[120],"that":[121,218,230],"can":[125,222],"readily":[127],"implemented,":[128],"characterized,":[129],"engineered":[131],"framework":[135,163],"consequently":[137],"transferred":[138],"We":[142,158,188,216],"implemented":[143],"characterized":[145],"\u201crepressilator\u201d":[147],"6":[148],",":[149,204],"three-node":[151,197],"negative":[152,171],"feedback":[153,172],"oscillator":[154],"then":[159],"used":[160],"our":[161,190],"engineer":[165],"three-node,":[167],"four-node,":[168],"five-node":[170,199],"architectures":[173],"going":[174],"from":[175],"circuit":[179,220],"analysis":[184],"complete":[186],"networks.":[187,266],"validated":[189],"by":[193],"transferring":[194],"these":[195],"oscillators":[200],"Escherichia":[202],"coli":[203],"resulting":[205],"robust":[207],"synchronized":[209],"oscillations":[210],"reflecting":[211],"observation.":[215],"demonstrate":[217],"comprehensive":[219],"performed":[224],"system":[228],"results":[234],"have":[235],"direct":[236],"applicability":[237],"vivo":[239],"Cell-free":[241],"biology":[243],"thus":[244],"has":[245],"potential":[247],"drastically":[249],"speed":[250],"up":[251],"design-build-test":[252],"cycles":[253],"enable":[258],"natural":[265]},"counts_by_year":[{"year":2026,"cited_by_count":13},{"year":2025,"cited_by_count":12},{"year":2024,"cited_by_count":12},{"year":2023,"cited_by_count":12},{"year":2022,"cited_by_count":19},{"year":2021,"cited_by_count":33},{"year":2020,"cited_by_count":28},{"year":2019,"cited_by_count":33},{"year":2018,"cited_by_count":27},{"year":2017,"cited_by_count":26},{"year":2016,"cited_by_count":14},{"year":2015,"cited_by_count":4}],"updated_date":"2026-11-09T13:22:03.289127","created_date":"2016-06-24T00:00:00"}