{"id":"https://openalex.org/W2119989237","doi":"https://doi.org/10.1093/oxfordjournals.jbchem.a122525","title":"Physicochemical Properties of Charge Isomers of Recombinant Human Superoxide Dismutase","display_name":"Physicochemical Properties of Charge Isomers of Recombinant Human Superoxide Dismutase","publication_year":1988,"publication_date":"1988-10-01","ids":{"openalex":"https://openalex.org/W2119989237","doi":"https://doi.org/10.1093/oxfordjournals.jbchem.a122525","mag":"2119989237","pmid":"https://pubmed.ncbi.nlm.nih.gov/3241003"},"language":"en","primary_location":{"id":"doi:10.1093/oxfordjournals.jbchem.a122525","doi":"https://doi.org/10.1093/oxfordjournals.jbchem.a122525","is_oa":false,"landing_page_url":"https://doi.org/10.1093/oxfordjournals.jbchem.a122525","pdf_url":null,"source":{"id":"https://openalex.org/S184547178","display_name":"The Journal of Biochemistry","issn_l":"0021-924X","issn":["0021-924X","1756-2651"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5043819847","display_name":"Jun-ichi Kajihara","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210150228","display_name":"Nippon Kayaku (Japan)","ror":"https://ror.org/05cbf4214","country_code":"JP","type":"company","lineage":["https://openalex.org/I4210150228"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Jun-ichi Kajihara","raw_affiliation_strings":["Pharmaceuticals Group, Nippon Kayaku Co., Ltd., Tokyo","Research Laboratories, Pharmaceuticals Group, Nippon Kayaku Co., Ltd.Kita-ku, Tokyo 115"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Pharmaceuticals Group, Nippon Kayaku Co., Ltd., Tokyo","institution_ids":["https://openalex.org/I4210150228"]},{"raw_affiliation_string":"Research Laboratories, Pharmaceuticals Group, Nippon Kayaku Co., Ltd.Kita-ku, Tokyo 115","institution_ids":["https://openalex.org/I4210150228"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5080303504","display_name":"Mitsuo Enomoto","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210150228","display_name":"Nippon Kayaku (Japan)","ror":"https://ror.org/05cbf4214","country_code":"JP","type":"company","lineage":["https://openalex.org/I4210150228"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Mitsuo Enomoto","raw_affiliation_strings":["Research Laboratories, Pharmaceuticals Group, Nippon Kayaku Co., Ltd.Kita-ku, Tokyo 115"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Research Laboratories, Pharmaceuticals Group, Nippon Kayaku Co., Ltd.Kita-ku, Tokyo 115","institution_ids":["https://openalex.org/I4210150228"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5020823195","display_name":"Kenji Seya","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210150228","display_name":"Nippon Kayaku (Japan)","ror":"https://ror.org/05cbf4214","country_code":"JP","type":"company","lineage":["https://openalex.org/I4210150228"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Kenji Seya","raw_affiliation_strings":["Research Laboratories, Pharmaceuticals Group, Nippon Kayaku Co., Ltd.Kita-ku, Tokyo 115"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Research Laboratories, Pharmaceuticals Group, Nippon Kayaku Co., Ltd.Kita-ku, Tokyo 115","institution_ids":["https://openalex.org/I4210150228"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5090460998","display_name":"Yoshikazu Sukenaga","orcid":"https://orcid.org/0000-0001-8479-3842","observed_orcids":["https://orcid.org/0000-0001-8479-3842"]},"institutions":[{"id":"https://openalex.org/I4210150228","display_name":"Nippon Kayaku (Japan)","ror":"https://ror.org/05cbf4214","country_code":"JP","type":"company","lineage":["https://openalex.org/I4210150228"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Yoshikazu Sukenaga","raw_affiliation_strings":["Research Laboratories, Pharmaceuticals Group, Nippon Kayaku Co., Ltd.Kita-ku, Tokyo 115"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Research Laboratories, Pharmaceuticals Group, Nippon Kayaku Co., Ltd.Kita-ku, Tokyo 115","institution_ids":["https://openalex.org/I4210150228"]}]},{"author_position":"last","author":{"id":null,"display_name":"Kazuo Katoh","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210150228","display_name":"Nippon Kayaku (Japan)","ror":"https://ror.org/05cbf4214","country_code":"JP","type":"company","lineage":["https://openalex.org/I4210150228"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"Kazuo Katoh","raw_affiliation_strings":["Research Laboratories, Pharmaceuticals Group, Nippon Kayaku Co., Ltd.Kita-ku, Tokyo 115"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Research Laboratories, Pharmaceuticals Group, Nippon Kayaku Co., Ltd.Kita-ku, Tokyo 115","institution_ids":["https://openalex.org/I4210150228"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I4210150228"],"apc_list":{"value":3276,"currency":"USD","value_usd":3276},"apc_paid":null,"fwci":0.446,"has_fulltext":false,"cited_by_count":28,"citation_normalized_percentile":{"value":0.58530778,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"104","issue":"4","first_page":"638","last_page":"642"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.37720000743865967,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.37720000743865967,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.22130000591278076,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11124","display_name":"Protein purification and stability","score":0.1534000039100647,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/superoxide-dismutase","display_name":"superoxide dismutase","score":0.9269999861717224},{"id":"https://openalex.org/keywords/charge-isomers","display_name":"charge isomers","score":0.8849999904632568},{"id":"https://openalex.org/keywords/isoelectric-point","display_name":"isoelectric point","score":0.8980000019073486},{"id":"https://openalex.org/keywords/disulfide-bonds","display_name":"disulfide bonds","score":0.7049999833106995},{"id":"https://openalex.org/keywords/cysteine-labeling","display_name":"cysteine labeling","score":0.7689999938011169},{"id":"https://openalex.org/keywords/protein-secondary-and-tertiary-structure","display_name":"protein secondary and tertiary structure","score":0.6769999861717224},{"id":"https://openalex.org/keywords/protein-heterogeneity","display_name":"protein heterogeneity","score":0.45500001311302185},{"id":"https://openalex.org/keywords/escherichia-coli-expression","display_name":"Escherichia coli expression","score":0.6899999976158142}],"concepts":[{"id":"https://openalex.org/C96294017","wikidata":"https://www.wikidata.org/wiki/Q308708","display_name":"Isoelectric point","level":3,"score":0.9075148701667786},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.8252788782119751},{"id":"https://openalex.org/C50476208","wikidata":"https://www.wikidata.org/wiki/Q898861","display_name":"Isoelectric focusing","level":3,"score":0.679951012134552},{"id":"https://openalex.org/C2779201268","wikidata":"https://www.wikidata.org/wiki/Q186474","display_name":"Cysteine","level":3,"score":0.5200357437133789},{"id":"https://openalex.org/C179998833","wikidata":"https://www.wikidata.org/wiki/Q381233","display_name":"High-performance liquid chromatography","level":2,"score":0.5156430006027222},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.4952484667301178},{"id":"https://openalex.org/C73826308","wikidata":"https://www.wikidata.org/wiki/Q684907","display_name":"Elution","level":2,"score":0.48432838916778564},{"id":"https://openalex.org/C51724486","wikidata":"https://www.wikidata.org/wiki/Q908955","display_name":"Column chromatography","level":2,"score":0.45984163880348206},{"id":"https://openalex.org/C2775838275","wikidata":"https://www.wikidata.org/wiki/Q410776","display_name":"Superoxide dismutase","level":3,"score":0.4422447979450226},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.4214092791080475},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.4151363968849182},{"id":"https://openalex.org/C27256138","wikidata":"https://www.wikidata.org/wiki/Q1230226","display_name":"Disulfide bond","level":2,"score":0.4144713282585144},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.3566650152206421},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.2448960840702057},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.22625979781150818},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000596","descriptor_name":"Amino Acids","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D002621","descriptor_name":"Chemistry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007527","descriptor_name":"Isoenzymes","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D008670","descriptor_name":"Metals","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D013057","descriptor_name":"Spectrum Analysis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013482","descriptor_name":"Superoxide Dismutase","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D055598","descriptor_name":"Chemical Phenomena","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1093/oxfordjournals.jbchem.a122525","doi":"https://doi.org/10.1093/oxfordjournals.jbchem.a122525","is_oa":false,"landing_page_url":"https://doi.org/10.1093/oxfordjournals.jbchem.a122525","pdf_url":null,"source":{"id":"https://openalex.org/S184547178","display_name":"The Journal of Biochemistry","issn_l":"0021-924X","issn":["0021-924X","1756-2651"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Biochemistry","raw_type":"journal-article"},{"id":"pmid:3241003","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/3241003","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of biochemistry","raw_type":"Comparative Study"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.933.460","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.933.460","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://jb.oxfordjournals.org/content/104/4/638.full.pdf","raw_type":"text"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W7100531955","https://openalex.org/W2343935996","https://openalex.org/W1764939414","https://openalex.org/W2069385550","https://openalex.org/W2063817683","https://openalex.org/W2009091539","https://openalex.org/W2042720670","https://openalex.org/W2153246200","https://openalex.org/W822020884","https://openalex.org/W1992470547"],"abstract_inverted_index":{"Recombinant":[0],"human":[1],"Cu2Zn2SOD":[2],"expressed":[3],"in":[4,39,81,89,97,146,152],"Escherichia":[5],"coli":[6],"consisted":[7],"of":[8,15,62,64,107,126,134],"mainly":[9],"three":[10,109,142],"isomers":[11,110,127,143],"with":[12,68],"isoelectric":[13,105],"points":[14,106],"5.14":[16],"(A),":[17],"5.06":[18],"(B),":[19],"and":[20,30,41,53,55,79,96,118,120],"4.99":[21],"(C).":[22],"Each":[23],"isomer":[24],"was":[25],"isolated":[26],"by":[27,116],"DEAE-Toyopearl":[28],"chromatography":[29],"the":[31,71,90,98,108,112,132,141],"physiochemical":[32],"properties":[33],"were":[34,58,87],"investigated.":[35],"No":[36],"significant":[37],"differences":[38,86,158],"chemical":[40],"spectrophotometric":[42],"properties,":[43],"such":[44],"as":[45],"specific":[46],"activity,":[47],"metal":[48],"contents,":[49],"amino":[50],"acid":[51],"composition,":[52],"UV":[54],"ESR":[56],"spectra,":[57],"found.":[59],"The":[60,104],"result":[61],"labeling":[63],"free":[65],"cysteine":[66],"residues":[67],"ABD-F":[69],"showed":[70],"disulfide":[72],"bond":[73],"to":[74,129],"be":[75,130],"formed":[76],"between":[77],"57Cys":[78],"146Cys":[80],"every":[82],"isomer.":[83],"A":[84],"few":[85],"found":[88],"CD":[91],"spectrum":[92],"around":[93,165],"260":[94],"nm":[95],"elution":[99],"patterns":[100],"on":[101],"reverse-phase":[102],"HPLC.":[103],"became":[111],"same":[113],"after":[114,122],"treatment":[115],"reduction":[117,123],"carboxymethylation":[119],"even":[121],"only,":[124],"pI":[125],"tended":[128],"at":[131],"value":[133],"component":[135],"(A).":[136],"These":[137,157],"results":[138],"suggest":[139],"that":[140],"are":[144,159],"identical":[145],"primary":[147],"structure":[148],"but":[149],"slightly":[150],"different":[151],"secondary":[153],"or":[154],"tertiary":[155],"structure.":[156],"probably":[160],"derived":[161],"from":[162],"structural":[163],"alterations":[164],"111Cys.":[166]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}