{"id":"https://openalex.org/W2118337647","doi":"https://doi.org/10.1093/nar/gng057","title":"An inbred 129SvEv GFPCre transgenic mouse that deletes loxP-flanked genes in all tissues","display_name":"An inbred 129SvEv GFPCre transgenic mouse that deletes loxP-flanked genes in all tissues","publication_year":2003,"publication_date":"2003-05-07","ids":{"openalex":"https://openalex.org/W2118337647","doi":"https://doi.org/10.1093/nar/gng057","mag":"2118337647","pmid":"https://pubmed.ncbi.nlm.nih.gov/12736323","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/156060"},"language":"en","primary_location":{"id":"doi:10.1093/nar/gng057","doi":"https://doi.org/10.1093/nar/gng057","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/gng057","pdf_url":"https://academic.oup.com/nar/article-pdf/31/10/e57/3973271/gng057.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://academic.oup.com/nar/article-pdf/31/10/e57/3973271/gng057.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5036326492","display_name":"John R. Scheel","orcid":"https://orcid.org/0000-0002-0998-0098","observed_orcids":["https://orcid.org/0000-0002-0998-0098"]},"institutions":[{"id":"https://openalex.org/I1307098950","display_name":"Salk Institute for Biological Studies","ror":"https://ror.org/03xez1567","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I1307098950"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"J. R. Scheel","raw_affiliation_strings":["The Salk Institute for Biological Studies, Laboratory of Genetics, 10010 North Torrey Pines Road, San Diego, CA 92037, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Salk Institute for Biological Studies, Laboratory of Genetics, 10010 North Torrey Pines Road, San Diego, CA 92037, USA","institution_ids":["https://openalex.org/I1307098950"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5036326492"],"corresponding_institution_ids":["https://openalex.org/I1307098950"],"apc_list":{"value":3992,"currency":"USD","value_usd":3992},"apc_paid":null,"fwci":0.8804,"has_fulltext":true,"cited_by_count":17,"citation_normalized_percentile":{"value":0.73662941,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"31","issue":"10","first_page":"57e","last_page":"57"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12929","display_name":"Animal Genetics and Reproduction","score":0.9711999893188477,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12929","display_name":"Animal Genetics and Reproduction","score":0.9711999893188477,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10878","display_name":"CRISPR and Genetic Engineering","score":0.027300000190734863,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10505","display_name":"Pluripotent Stem Cells Research","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/cre-loxp-recombination","display_name":"Cre-loxP recombination","score":0.7179999947547913},{"id":"https://openalex.org/keywords/conditional-gene-deletion","display_name":"conditional gene deletion","score":0.5580000281333923}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8795554637908936},{"id":"https://openalex.org/C125929170","wikidata":"https://www.wikidata.org/wiki/Q908966","display_name":"Cre recombinase","level":5,"score":0.7526307702064514},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.711488664150238},{"id":"https://openalex.org/C111829913","wikidata":"https://www.wikidata.org/wiki/Q211083","display_name":"Gene targeting","level":3,"score":0.6452242136001587},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.6355421543121338},{"id":"https://openalex.org/C42411736","wikidata":"https://www.wikidata.org/wiki/Q1550327","display_name":"Selectable marker","level":4,"score":0.6310093998908997},{"id":"https://openalex.org/C102230213","wikidata":"https://www.wikidata.org/wiki/Q430117","display_name":"Transgene","level":3,"score":0.6119226813316345},{"id":"https://openalex.org/C38027674","wikidata":"https://www.wikidata.org/wiki/Q4994856","display_name":"Cre-Lox recombination","level":5,"score":0.5659269094467163},{"id":"https://openalex.org/C109825262","wikidata":"https://www.wikidata.org/wiki/Q1139164","display_name":"Germline","level":3,"score":0.539006233215332},{"id":"https://openalex.org/C84597430","wikidata":"https://www.wikidata.org/wiki/Q106227","display_name":"Locus (genetics)","level":3,"score":0.5016012191772461},{"id":"https://openalex.org/C141035611","wikidata":"https://www.wikidata.org/wiki/Q5532934","display_name":"Genetically modified mouse","level":4,"score":0.3251877427101135}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001665","descriptor_name":"Binding Sites","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D004351","descriptor_name":"Drug Resistance","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006720","descriptor_name":"Homozygote","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007041","descriptor_name":"Hypoxanthine Phosphoribosyltransferase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D008164","descriptor_name":"Luminescent Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D008164","descriptor_name":"Luminescent Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008815","descriptor_name":"Mice, Inbred Strains","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008822","descriptor_name":"Mice, Transgenic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011995","descriptor_name":"Recombination, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014764","descriptor_name":"Viral Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D014764","descriptor_name":"Viral Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D016254","descriptor_name":"Mutagenesis, Insertional","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016678","descriptor_name":"Genome","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017353","descriptor_name":"Gene Deletion","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D018613","descriptor_name":"Microscopy, Confocal","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019426","descriptor_name":"Integrases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D019426","descriptor_name":"Integrases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D049452","descriptor_name":"Green Fluorescent Proteins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1093/nar/gng057","doi":"https://doi.org/10.1093/nar/gng057","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/gng057","pdf_url":"https://academic.oup.com/nar/article-pdf/31/10/e57/3973271/gng057.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:156060","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/156060","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Res","raw_type":"Text"},{"id":"pmid:12736323","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12736323","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic acids research","raw_type":"Journal Article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.491.7655","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.491.7655","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://nar.oxfordjournals.org/content/31/10/e57.full.pdf","raw_type":"text"}],"best_oa_location":{"id":"doi:10.1093/nar/gng057","doi":"https://doi.org/10.1093/nar/gng057","is_oa":true,"landing_page_url":"https://doi.org/10.1093/nar/gng057","pdf_url":"https://academic.oup.com/nar/article-pdf/31/10/e57/3973271/gng057.pdf","source":{"id":"https://openalex.org/S134668137","display_name":"Nucleic Acids Research","issn_l":"0305-1048","issn":["0305-1048","1362-4954","1362-4962"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-3","ki-jl-3","medline","norway-2","oarl","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Nucleic Acids Research","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.504800021648407,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"sustainable_development_goals_aurora":[{"score":0.8399999737739563,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G1626841135","display_name":null,"funder_award_id":"F31 NS10860-01","funder_id":"https://openalex.org/F4320337359","funder_display_name":"National Institute of Neurological Disorders and Stroke"},{"id":"https://openalex.org/G4971353333","display_name":"MINORITY PREDOCTORAL FELLOWSHIP","funder_award_id":"F31NS010860","funder_id":"https://openalex.org/F4320337359","funder_display_name":"National Institute of Neurological Disorders and Stroke"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337359","display_name":"National Institute of Neurological Disorders and Stroke","ror":"https://ror.org/01s5ya894"}],"has_content":{"grobid_xml":true,"pdf":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2118337647.pdf","grobid_xml":"https://content.openalex.org/works/W2118337647.grobid-xml"},"referenced_works_count":11,"referenced_works":["https://openalex.org/W1484163259","https://openalex.org/W1563777667","https://openalex.org/W1588949603","https://openalex.org/W1617122023","https://openalex.org/W1964215549","https://openalex.org/W1972160020","https://openalex.org/W2039525141","https://openalex.org/W2074395616","https://openalex.org/W2091275170","https://openalex.org/W2116010079","https://openalex.org/W2418735187"],"related_works":["https://openalex.org/W1968963646","https://openalex.org/W539833","https://openalex.org/W2091275170","https://openalex.org/W2058172887","https://openalex.org/W1986582839","https://openalex.org/W2088776250","https://openalex.org/W2074395616","https://openalex.org/W1871312389","https://openalex.org/W2115847473","https://openalex.org/W4388311037"],"abstract_inverted_index":{"A":[0],"common":[1],"method":[2,87],"for":[3,124,131,142],"generating":[4],"mice":[5,121,159],"with":[6,24,36,55],"subtle":[7],"genetic":[8],"manipulations":[9],"uses":[10],"homologous":[11],"recombination":[12],"(HR)":[13],"in":[14,113,134,181],"embryonic":[15],"stem":[16],"(ES)":[17],"cells":[18],"to":[19,40,89,96,106,149,155],"replace":[20],"a":[21,25,30,61,91,101,173],"wild-type":[22],"gene":[23,33,39,53,105],"slightly":[26],"modified":[27,38],"one.":[28],"Generally,":[29],"drug":[31,51,79,103,178],"resistance":[32,52,104,179],"is":[34,95],"inserted":[35],"the":[37,47,56,68,114,139,143,164,185,196],"select":[41],"correctly":[42],"targeted":[43],"clones.":[44],"Often,":[45],"however,":[46],"presence":[48],"of":[49,67,145,166,191],"this":[50,83,125],"interferes":[54],"normal":[57],"locus":[58],"and":[59,141],"creates":[60],"null":[62],"or":[63],"hypomorphic":[64],"allele.":[65],"Flanking":[66],"selectable":[69,93],"marker":[70,94],"by":[71,75],"loxP":[72],"sites":[73],"followed":[74],"Cre-mediated":[76],"deletion":[77,190],"after":[78],"selection":[80],"can":[81,128],"overcome":[82],"problem.":[84],"The":[85],"simplest":[86],"used":[88],"remove":[90],"loxP-flanked":[92,102,177,192],"breed":[97],"an":[98,107,199],"animal":[99,108],"carrying":[100],"that":[109,160,175],"expresses":[110],"Cre":[111,162],"recombinase":[112],"germline.":[115,186],"To":[116],"date":[117],"only":[118],"outbred":[119],"transgenic":[120],"are":[122],"available":[123],"purpose.":[126],"This":[127,187],"be":[129],"problematic":[130],"phenotypic":[132],"analysis":[133,144],"many":[135],"organ":[136],"systems,":[137],"including":[138,184],"brain,":[140],"behavior.":[146],"While":[147],"attempting":[148],"make":[150],"129S6/SvEvTac":[151],"inbred":[152,200],"background":[153],"(isogenic":[154],"our":[156],"ES":[157],"cells)":[158],"express":[161],"under":[163],"control":[165],"several":[167],"tissue-specific":[168],"promoters,":[169],"we":[170],"serendipitously":[171],"generated":[172],"line":[174],"excises":[176],"genes":[180],"all":[182],"tissues,":[183],"reagent":[188],"allows":[189],"sequences":[193],"while":[194],"maintaining":[195],"mutation":[197],"on":[198],"background.":[201]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2018,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}