{"id":"https://openalex.org/W2118143753","doi":"https://doi.org/10.3109/19401736.2015.1060475","title":"Development of multiplex PCR assay for authentication of Cornu Cervi Pantotrichum in traditional Chinese medicine based on cytochrome b and C oxidase subunit 1 genes","display_name":"Development of multiplex PCR assay for authentication of Cornu Cervi Pantotrichum in traditional Chinese medicine based on cytochrome b and C oxidase subunit 1 genes","publication_year":2015,"publication_date":"2015-08-17","ids":{"openalex":"https://openalex.org/W2118143753","doi":"https://doi.org/10.3109/19401736.2015.1060475","mag":"2118143753","pmid":"https://pubmed.ncbi.nlm.nih.gov/26287950"},"language":"en","primary_location":{"id":"doi:10.3109/19401736.2015.1060475","doi":"https://doi.org/10.3109/19401736.2015.1060475","is_oa":false,"landing_page_url":"https://doi.org/10.3109/19401736.2015.1060475","pdf_url":null,"source":{"id":"https://openalex.org/S4210196298","display_name":"Mitochondrial DNA Part A","issn_l":"2470-1394","issn":["2470-1394","2470-1408"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","medline","norway-1","poland-20","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Mitochondrial DNA Part A","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5101670654","display_name":"Lijun Gao","orcid":"https://orcid.org/0000-0001-6478-3305","observed_orcids":["https://orcid.org/0000-0001-6478-3305"]},"institutions":[{"id":"https://openalex.org/I121691239","display_name":"Beihua University","ror":"https://ror.org/013jjp941","country_code":"CN","type":"education","lineage":["https://openalex.org/I121691239"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Lijun Gao","raw_affiliation_strings":["School of Laboratory Medicine, Beihua University, Jilin, People\u2019s Republic of China,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Laboratory Medicine, Beihua University, Jilin, People\u2019s Republic of China,","institution_ids":["https://openalex.org/I121691239"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100654146","display_name":"Wei Xia","orcid":"https://orcid.org/0000-0001-5005-059X","observed_orcids":["https://orcid.org/0000-0001-5005-059X"]},"institutions":[{"id":"https://openalex.org/I121691239","display_name":"Beihua University","ror":"https://ror.org/013jjp941","country_code":"CN","type":"education","lineage":["https://openalex.org/I121691239"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Wei Xia","raw_affiliation_strings":["School of Laboratory Medicine, Beihua University, Jilin, People\u2019s Republic of China,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Laboratory Medicine, Beihua University, Jilin, People\u2019s Republic of China,","institution_ids":["https://openalex.org/I121691239"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5079587208","display_name":"Jinxia Ai","orcid":"https://orcid.org/0009-0001-3607-5704","observed_orcids":["https://orcid.org/0009-0001-3607-5704"]},"institutions":[{"id":"https://openalex.org/I121691239","display_name":"Beihua University","ror":"https://ror.org/013jjp941","country_code":"CN","type":"education","lineage":["https://openalex.org/I121691239"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Jinxia Ai","raw_affiliation_strings":["School of Laboratory Medicine, Beihua University, Jilin, People\u2019s Republic of China,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Laboratory Medicine, Beihua University, Jilin, People\u2019s Republic of China,","institution_ids":["https://openalex.org/I121691239"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5028133176","display_name":"Mingcheng Li","orcid":"https://orcid.org/0000-0003-2640-4305","observed_orcids":["https://orcid.org/0000-0003-2640-4305"]},"institutions":[{"id":"https://openalex.org/I121691239","display_name":"Beihua University","ror":"https://ror.org/013jjp941","country_code":"CN","type":"education","lineage":["https://openalex.org/I121691239"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Mingcheng Li","raw_affiliation_strings":["School of Laboratory Medicine, Beihua University, Jilin, People\u2019s Republic of China,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Laboratory Medicine, Beihua University, Jilin, People\u2019s Republic of China,","institution_ids":["https://openalex.org/I121691239"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5000436052","display_name":"Guangxin Yuan","orcid":"https://orcid.org/0000-0003-1333-8767","observed_orcids":["https://orcid.org/0000-0003-1333-8767"]},"institutions":[{"id":"https://openalex.org/I121691239","display_name":"Beihua University","ror":"https://ror.org/013jjp941","country_code":"CN","type":"education","lineage":["https://openalex.org/I121691239"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Guanxin Yuan","raw_affiliation_strings":["School of Pharmacy, Beihua University, Jilin, People\u2019s Republic of China, and"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Pharmacy, Beihua University, Jilin, People\u2019s Republic of China, and","institution_ids":["https://openalex.org/I121691239"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5027805081","display_name":"Jiamu Niu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I121691239","display_name":"Beihua University","ror":"https://ror.org/013jjp941","country_code":"CN","type":"education","lineage":["https://openalex.org/I121691239"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Jiamu Niu","raw_affiliation_strings":["School of Laboratory Medicine, Beihua University, Jilin, People\u2019s Republic of China,"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"School of Laboratory Medicine, Beihua University, Jilin, People\u2019s Republic of China,","institution_ids":["https://openalex.org/I121691239"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5086575157","display_name":"Guilian Fu","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Guilian Fu","raw_affiliation_strings":["Jilin Leibo Scientific Co. Ltd, Jilin, People\u2019s Republic of China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Jilin Leibo Scientific Co. Ltd, Jilin, People\u2019s Republic of China","institution_ids":[]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5100414909","display_name":"Lihua Zhang","orcid":"https://orcid.org/0000-0003-2543-1547","observed_orcids":["https://orcid.org/0000-0003-2543-1547","https://orcid.org/0009-0001-4582-8958","https://orcid.org/0000-0002-6880-676X"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"Lihua Zhang","raw_affiliation_strings":["Jilin Leibo Scientific Co. Ltd, Jilin, People\u2019s Republic of China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Jilin Leibo Scientific Co. Ltd, Jilin, People\u2019s Republic of China","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5100654146"],"corresponding_institution_ids":["https://openalex.org/I121691239"],"apc_list":null,"apc_paid":null,"fwci":0.4235,"has_fulltext":false,"cited_by_count":10,"citation_normalized_percentile":{"value":0.53809684,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":95},"biblio":{"volume":"27","issue":"4","first_page":"2989","last_page":"2992"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12388","display_name":"Identification and Quantification in Food","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12388","display_name":"Identification and Quantification in Food","score":1.0,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11709","display_name":"Traditional Chinese Medicine Analysis","score":9.999999747378752e-05,"subfield":{"id":"https://openalex.org/subfields/2707","display_name":"Complementary and alternative medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T12878","display_name":"Pharmaceutical Quality and Counterfeiting","score":0.0,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/deer-antler","display_name":"deer antler","score":0.6200000047683716},{"id":"https://openalex.org/keywords/traditional-chinese-medicine","display_name":"traditional Chinese medicine","score":0.8169999718666077},{"id":"https://openalex.org/keywords/multiplex-pcr","display_name":"multiplex PCR","score":0.9300000071525574},{"id":"https://openalex.org/keywords/cytochrome-b","display_name":"cytochrome b","score":0.925000011920929},{"id":"https://openalex.org/keywords/cytochrome-c-oxidase-subunit-i","display_name":"cytochrome c oxidase subunit I","score":0.9670000076293945},{"id":"https://openalex.org/keywords/mitochondrial-dna","display_name":"mitochondrial DNA","score":0.9309999942779541},{"id":"https://openalex.org/keywords/species-identification","display_name":"species identification","score":0.6039999723434448}],"concepts":[{"id":"https://openalex.org/C24586158","wikidata":"https://www.wikidata.org/wiki/Q27075","display_name":"Mitochondrial DNA","level":3,"score":0.7570756673812866},{"id":"https://openalex.org/C90583042","wikidata":"https://www.wikidata.org/wiki/Q1952456","display_name":"Multiplex polymerase chain reaction","level":4,"score":0.6906583309173584},{"id":"https://openalex.org/C2778200843","wikidata":"https://www.wikidata.org/wiki/Q14865314","display_name":"Cytochrome c oxidase subunit I","level":4,"score":0.6821687817573547},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6813825368881226},{"id":"https://openalex.org/C17757408","wikidata":"https://www.wikidata.org/wiki/Q4135516","display_name":"genomic DNA","level":3,"score":0.6308685541152954},{"id":"https://openalex.org/C123249941","wikidata":"https://www.wikidata.org/wiki/Q306116","display_name":"Cytochrome c oxidase","level":3,"score":0.6239883303642273},{"id":"https://openalex.org/C2777563447","wikidata":"https://www.wikidata.org/wiki/Q7243397","display_name":"Primer (cosmetics)","level":2,"score":0.6148597598075867},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.613111674785614},{"id":"https://openalex.org/C148292235","wikidata":"https://www.wikidata.org/wiki/Q3774486","display_name":"Cytochrome b","level":4,"score":0.4896705448627472},{"id":"https://openalex.org/C49105822","wikidata":"https://www.wikidata.org/wiki/Q176996","display_name":"Polymerase chain reaction","level":3,"score":0.4821617007255554},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.4792344868183136},{"id":"https://openalex.org/C2781188995","wikidata":"https://www.wikidata.org/wiki/Q6934982","display_name":"Multiplex","level":2,"score":0.47259148955345154},{"id":"https://openalex.org/C33935397","wikidata":"https://www.wikidata.org/wiki/Q7915779","display_name":"Variants of PCR","level":4,"score":0.4474732577800751},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.4398542642593384},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.35962459444999695},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.10706153512001038},{"id":"https://openalex.org/C28859421","wikidata":"https://www.wikidata.org/wiki/Q39572","display_name":"Mitochondrion","level":2,"score":0.0912134051322937},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000999","descriptor_name":"Antlers","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D003670","descriptor_name":"Deer","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D004272","descriptor_name":"DNA, Mitochondrial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008516","descriptor_name":"Medicine, Chinese Traditional","qualifier_ui":"Q000592","qualifier_name":"standards","is_major_topic":true},{"descriptor_ui":"D010088","descriptor_name":"Oxidoreductases","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D045303","descriptor_name":"Cytochromes b","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D045304","descriptor_name":"Cytochromes c","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D054629","descriptor_name":"Genome, Mitochondrial","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D060885","descriptor_name":"Multiplex Polymerase Chain Reaction","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.3109/19401736.2015.1060475","doi":"https://doi.org/10.3109/19401736.2015.1060475","is_oa":false,"landing_page_url":"https://doi.org/10.3109/19401736.2015.1060475","pdf_url":null,"source":{"id":"https://openalex.org/S4210196298","display_name":"Mitochondrial DNA Part A","issn_l":"2470-1394","issn":["2470-1394","2470-1408"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","medline","norway-1","poland-20","russia-white-list-3"],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Mitochondrial DNA Part A","raw_type":"journal-article"},{"id":"pmid:26287950","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/26287950","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":19,"referenced_works":["https://openalex.org/W194576830","https://openalex.org/W1974877624","https://openalex.org/W1980922304","https://openalex.org/W1981068259","https://openalex.org/W2005943848","https://openalex.org/W2024513219","https://openalex.org/W2036365984","https://openalex.org/W2057019074","https://openalex.org/W2065541108","https://openalex.org/W2093419633","https://openalex.org/W2094048117","https://openalex.org/W2113642274","https://openalex.org/W2138193047","https://openalex.org/W2352652245","https://openalex.org/W2381554101","https://openalex.org/W2405758322","https://openalex.org/W2776587137","https://openalex.org/W2883871681","https://openalex.org/W4285719527"],"related_works":["https://openalex.org/W6940104238","https://openalex.org/W3022549003","https://openalex.org/W2776587137","https://openalex.org/W2974931573","https://openalex.org/W2608881761","https://openalex.org/W2044056817","https://openalex.org/W2294665926","https://openalex.org/W2357615117","https://openalex.org/W192169953","https://openalex.org/W2019281282"],"abstract_inverted_index":{"This":[0],"study":[1],"describes":[2],"a":[3,102,134],"method":[4,62],"for":[5,27,120],"discriminating":[6],"the":[7,23,56,60,109],"true":[8,97],"Cervus":[9,98,127],"antlers":[10,99,132],"from":[11,51,74,94,130],"its":[12,66],"counterfeits":[13],"using":[14,113],"multiplex":[15,110],"PCR.":[16],"Bioinformatics":[17],"were":[18,49,72,91],"carried":[19],"out":[20],"to":[21,65],"design":[22],"specific":[24],"alleles":[25],"primers":[26],"mitochondrial":[28],"(mt)":[29],"cytochrome":[30,35],"b":[31],"(Cyt":[32],"b)":[33],"and":[34,46,59,81,87,122],"C":[36],"oxidase":[37],"subunit":[38],"1":[39],"(Cox":[40],"1)":[41],"genes.":[42],"The":[43,105],"mt":[44],"DNA":[45,48,70],"genomic":[47],"extracted":[50,73],"Cervi":[52],"Cornu":[53],"Pantotrichum":[54],"through":[55],"modified":[57],"alkaline":[58],"salt-extracting":[61],"in":[63,78,133],"addition":[64],"counterfeits,":[67],"respectively.":[68],"Sufficient":[69],"templates":[71],"all":[75],"samples":[76],"used":[77,119],"two":[79,114],"methods,":[80],"joint":[82],"fragments":[83],"of":[84,96,125],"354":[85],"bp":[86,89],"543":[88],"that":[90,108],"specifically":[92],"amplified":[93],"both":[95],"served":[100],"as":[101],"standard":[103],"control.":[104],"data":[106],"revealed":[107],"PCR-based":[111],"assays":[112],"primer":[115],"sets":[116],"can":[117],"be":[118],"forensic":[121],"quantitative":[123],"identification":[124],"original":[126],"deer":[128],"products":[129],"counterfeit":[131],"single":[135],"step.":[136]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2023,"cited_by_count":2},{"year":2022,"cited_by_count":1},{"year":2020,"cited_by_count":2},{"year":2018,"cited_by_count":2},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}