{"id":"https://openalex.org/W2117470898","doi":"https://doi.org/10.1002/bit.260380114","title":"Protease\u2010catalyzed peptide synthesis using inverse substrates: The influence of reaction conditions on the trypsin acyl transfer efficiency","display_name":"Protease\u2010catalyzed peptide synthesis using inverse substrates: The influence of reaction conditions on the trypsin acyl transfer efficiency","publication_year":1991,"publication_date":"1991-06-05","ids":{"openalex":"https://openalex.org/W2117470898","doi":"https://doi.org/10.1002/bit.260380114","mag":"2117470898","pmid":"https://pubmed.ncbi.nlm.nih.gov/18600704"},"language":"en","primary_location":{"id":"doi:10.1002/bit.260380114","doi":"https://doi.org/10.1002/bit.260380114","is_oa":false,"landing_page_url":"https://doi.org/10.1002/bit.260380114","pdf_url":null,"source":{"id":"https://openalex.org/S151564723","display_name":"Biotechnology and Bioengineering","issn_l":"0006-3592","issn":["0006-3592","1097-0290"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Bioengineering","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5018783910","display_name":"Volker Schellenberger","orcid":"https://orcid.org/0000-0002-3491-8229","observed_orcids":["https://orcid.org/0000-0002-3491-8229"]},"institutions":[{"id":"https://openalex.org/I926574661","display_name":"Leipzig University","ror":"https://ror.org/03s7gtk40","country_code":"DE","type":"education","lineage":["https://openalex.org/I926574661"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Volker Schellenberger","raw_affiliation_strings":["Karl Marx University, Biosciences Division, Department of Biochemistry, Leipzig, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Karl Marx University, Biosciences Division, Department of Biochemistry, Leipzig, Germany","institution_ids":["https://openalex.org/I926574661"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5047363996","display_name":"Hans\u2010Dieter Jakubke","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I926574661","display_name":"Leipzig University","ror":"https://ror.org/03s7gtk40","country_code":"DE","type":"education","lineage":["https://openalex.org/I926574661"]}],"countries":["DE"],"is_corresponding":false,"raw_author_name":"Hans\u2010Dieter Jakubke","raw_affiliation_strings":["Karl Marx University, Biosciences Division, Department of Biochemistry, DDR-7010 Leipzig, Germany"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Karl Marx University, Biosciences Division, Department of Biochemistry, DDR-7010 Leipzig, Germany","institution_ids":["https://openalex.org/I926574661"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5077991462","display_name":"Nina P. Zapevalova","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210137602","display_name":"Institute of Protein Research","ror":"https://ror.org/0489f0s93","country_code":"RU","type":"facility","lineage":["https://openalex.org/I1313323035","https://openalex.org/I4210129690","https://openalex.org/I4210137602"]},{"id":"https://openalex.org/I4405285364","display_name":"Academy of Sciences of the Soviet Union","ror":"https://ror.org/059mhqh10","country_code":"RU","type":"government","lineage":["https://openalex.org/I1313323035","https://openalex.org/I4405285364"]}],"countries":["RU"],"is_corresponding":false,"raw_author_name":"Nina P. Zapevalova","raw_affiliation_strings":["Institute of Protein Research, Academy of Sciences of the USSR, 142292 Pushchino, Moscow Region, USSR"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Protein Research, Academy of Sciences of the USSR, 142292 Pushchino, Moscow Region, USSR","institution_ids":["https://openalex.org/I4210137602","https://openalex.org/I4405285364"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5109914370","display_name":"Yuri V. Mitin","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210137602","display_name":"Institute of Protein Research","ror":"https://ror.org/0489f0s93","country_code":"RU","type":"facility","lineage":["https://openalex.org/I1313323035","https://openalex.org/I4210129690","https://openalex.org/I4210137602"]},{"id":"https://openalex.org/I4405285364","display_name":"Academy of Sciences of the Soviet Union","ror":"https://ror.org/059mhqh10","country_code":"RU","type":"government","lineage":["https://openalex.org/I1313323035","https://openalex.org/I4405285364"]}],"countries":["RU"],"is_corresponding":true,"raw_author_name":"Yuri V. Mitin","raw_affiliation_strings":["Institute of Protein Research, Academy of Sciences of the USSR, 142292 Pushchino, Moscow Region, USSR"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Protein Research, Academy of Sciences of the USSR, 142292 Pushchino, Moscow Region, USSR","institution_ids":["https://openalex.org/I4210137602","https://openalex.org/I4405285364"]}]}],"institutions":[],"countries_distinct_count":2,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5109914370"],"corresponding_institution_ids":["https://openalex.org/I4210137602","https://openalex.org/I4405285364"],"apc_list":{"value":5060,"currency":"USD","value_usd":5060},"apc_paid":null,"fwci":0.9248,"has_fulltext":false,"cited_by_count":48,"citation_normalized_percentile":{"value":0.73699156,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"38","issue":"1","first_page":"104","last_page":"108"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":0.861299991607666,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10911","display_name":"Chemical Synthesis and Analysis","score":0.861299991607666,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.11699999868869781,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T13326","display_name":"Biochemical and Structural Characterization","score":0.007799999788403511,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/trypsin","display_name":"trypsin","score":0.9850000143051147},{"id":"https://openalex.org/keywords/reaction-conditions","display_name":"reaction conditions","score":0.6370000243186951},{"id":"https://openalex.org/keywords/ph-effect","display_name":"pH effect","score":0.7009999752044678},{"id":"https://openalex.org/keywords/cosolvent-effects","display_name":"cosolvent effects","score":0.9399999976158142},{"id":"https://openalex.org/keywords/deacylation","display_name":"deacylation","score":0.7990000247955322}],"concepts":[{"id":"https://openalex.org/C2780340462","wikidata":"https://www.wikidata.org/wiki/Q127450","display_name":"Trypsin","level":3,"score":0.6754612922668457},{"id":"https://openalex.org/C2776714187","wikidata":"https://www.wikidata.org/wiki/Q212410","display_name":"Protease","level":3,"score":0.6583501100540161},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6008332967758179},{"id":"https://openalex.org/C161790260","wikidata":"https://www.wikidata.org/wiki/Q82264","display_name":"Catalysis","level":2,"score":0.5276519060134888},{"id":"https://openalex.org/C2779281246","wikidata":"https://www.wikidata.org/wiki/Q172847","display_name":"Peptide","level":2,"score":0.5071513652801514},{"id":"https://openalex.org/C207467116","wikidata":"https://www.wikidata.org/wiki/Q4385666","display_name":"Inverse","level":2,"score":0.5004739761352539},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.36889803409576416},{"id":"https://openalex.org/C21951064","wikidata":"https://www.wikidata.org/wiki/Q899212","display_name":"Combinatorial chemistry","level":1,"score":0.3659772276878357},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.34599149227142334},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.3366459906101227},{"id":"https://openalex.org/C33923547","wikidata":"https://www.wikidata.org/wiki/Q395","display_name":"Mathematics","level":0,"score":0.0},{"id":"https://openalex.org/C2524010","wikidata":"https://www.wikidata.org/wiki/Q8087","display_name":"Geometry","level":1,"score":0.0}],"mesh":[],"locations_count":2,"locations":[{"id":"doi:10.1002/bit.260380114","doi":"https://doi.org/10.1002/bit.260380114","is_oa":false,"landing_page_url":"https://doi.org/10.1002/bit.260380114","pdf_url":null,"source":{"id":"https://openalex.org/S151564723","display_name":"Biotechnology and Bioengineering","issn_l":"0006-3592","issn":["0006-3592","1097-0290"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","jufo-2","ki-jl-1","medline","norway-1","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and Bioengineering","raw_type":"journal-article"},{"id":"pmid:18600704","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/18600704","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biotechnology and bioengineering","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":20,"referenced_works":["https://openalex.org/W617205846","https://openalex.org/W1959911680","https://openalex.org/W1974926247","https://openalex.org/W1983279218","https://openalex.org/W1985469117","https://openalex.org/W1986457099","https://openalex.org/W2001262910","https://openalex.org/W2015391756","https://openalex.org/W2023268443","https://openalex.org/W2029521241","https://openalex.org/W2031817642","https://openalex.org/W2058819042","https://openalex.org/W2068943002","https://openalex.org/W2088472608","https://openalex.org/W2140872076","https://openalex.org/W2156065136","https://openalex.org/W2203848431","https://openalex.org/W2833117375","https://openalex.org/W3133215333","https://openalex.org/W4285719527"],"related_works":["https://openalex.org/W2022878105","https://openalex.org/W1982046868","https://openalex.org/W2042091418","https://openalex.org/W2023925429","https://openalex.org/W2066004031","https://openalex.org/W1991277132","https://openalex.org/W1967533956","https://openalex.org/W1959911680","https://openalex.org/W2011898381","https://openalex.org/W2072614126"],"abstract_inverted_index":{"Benzyloxycarbonyl-L-alanine":[0],"p-guanidinophenyl":[1],"ester":[2],"behaves":[3],"as":[4,29,65,67],"a":[5,10],"trypsin":[6,33],"\"inverse":[7],"substrate,\"":[8],"i.e.,":[9],"cationic":[11],"center":[12],"is":[13,102],"included":[14],"in":[15,22,100],"the":[16,23,35,78,83,104],"leaving":[17],"group":[18],"instead":[19],"of":[20,37,64,74,92,96],"being":[21],"acyl":[24,31,49,79],"moiety.":[25],"Using":[26],"this":[27,45],"substrate":[28],"an":[30],"donor,":[32],"catalyzes":[34],"synthesis":[36],"peptide":[38],"bonds":[39],"that":[40],"cannot":[41],"be":[42,73],"split":[43],"by":[44,87],"enzyme.":[46],"An":[47],"optimal":[48],"transfer":[50,80],"efficiency":[51,95],"was":[52,70],"achieved":[53],"between":[54],"pH":[55],"8":[56],"and":[57,98,108],"9":[58],"at":[59],"30":[60],"degrees":[61],"C.The":[62],"addition":[63],"much":[66],"50%":[68],"cosolvent":[69],"shown":[71],"to":[72],"minor":[75],"influence":[76],"on":[77],"efficiency,":[81],"whereas":[82],"reaction":[84],"velocity":[85],"decreases":[86],"more":[88],"than":[89],"one":[90],"order":[91],"magnitude.":[93],"The":[94],"H-Leu-NH(2)":[97],"H-Val-NH(2)":[99],"deacylation":[101],"almost":[103],"same":[105],"for":[106],"\"inverse\"":[107],"normal":[109],"type":[110],"substrates.":[111]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2019,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-30T12:39:25.321706","created_date":"2016-06-24T00:00:00"}
