{"id":"https://openalex.org/W2116444313","doi":"https://doi.org/10.1128/ec.4.10.1613-1619.2005","title":"Analysis of Paramecium tetraurelia A-51 Surface Antigen Gene Mutants Reveals Positive-Feedback Mechanisms for Maintenance of Expression and Temperature-Induced Activation","display_name":"Analysis of Paramecium tetraurelia A-51 Surface Antigen Gene Mutants Reveals Positive-Feedback Mechanisms for Maintenance of Expression and Temperature-Induced Activation","publication_year":2005,"publication_date":"2005-10-01","ids":{"openalex":"https://openalex.org/W2116444313","doi":"https://doi.org/10.1128/ec.4.10.1613-1619.2005","mag":"2116444313","pmid":"https://pubmed.ncbi.nlm.nih.gov/16215168","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/1265893"},"language":"en","primary_location":{"id":"doi:10.1128/ec.4.10.1613-1619.2005","doi":"https://doi.org/10.1128/ec.4.10.1613-1619.2005","is_oa":true,"landing_page_url":"https://doi.org/10.1128/ec.4.10.1613-1619.2005","pdf_url":null,"source":{"id":"https://openalex.org/S177547185","display_name":"Eukaryotic Cell","issn_l":"1535-9778","issn":["1535-9778","1535-9786"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","norway-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Eukaryotic Cell","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"diamond","oa_url":"https://doi.org/10.1128/ec.4.10.1613-1619.2005","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5051819621","display_name":"Atsushi Matsuda","orcid":"https://orcid.org/0000-0003-0510-213X","observed_orcids":["https://orcid.org/0000-0003-0510-213X"]},"institutions":[{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Atsushi Matsuda","raw_affiliation_strings":["Department of Biochemistry, Purdue University, 175 S. University Street, West Lafayette, Indiana 47907-2063"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Purdue University, 175 S. University Street, West Lafayette, Indiana 47907-2063","institution_ids":["https://openalex.org/I219193219"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5082657792","display_name":"JAMES D. FORNEY","orcid":"https://orcid.org/0000-0002-6144-732X","observed_orcids":["https://orcid.org/0000-0002-6144-732X"]},"institutions":[{"id":"https://openalex.org/I219193219","display_name":"Purdue University West Lafayette","ror":"https://ror.org/02dqehb95","country_code":"US","type":"education","lineage":["https://openalex.org/I219193219"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"James D. Forney","raw_affiliation_strings":["Department of Biochemistry, Purdue University, 175 S. University Street, West Lafayette, Indiana 47907-2063"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Biochemistry, Purdue University, 175 S. University Street, West Lafayette, Indiana 47907-2063","institution_ids":["https://openalex.org/I219193219"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5082657792"],"corresponding_institution_ids":["https://openalex.org/I219193219"],"apc_list":null,"apc_paid":null,"fwci":0.1255,"has_fulltext":false,"cited_by_count":10,"citation_normalized_percentile":{"value":0.4804767,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":97},"biblio":{"volume":"4","issue":"10","first_page":"1613","last_page":"1619"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10222","display_name":"Genomics and Chromatin Dynamics","score":0.29589998722076416,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10222","display_name":"Genomics and Chromatin Dynamics","score":0.29589998722076416,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10434","display_name":"Chromosomal and Genetic Variations","score":0.15309999883174896,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10268","display_name":"Developmental Biology and Gene Regulation","score":0.05909999832510948,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/paramecium-tetraurelia","display_name":"Paramecium tetraurelia","score":0.9980000257492065},{"id":"https://openalex.org/keywords/variable-surface-antigens","display_name":"variable surface antigens","score":0.8949999809265137},{"id":"https://openalex.org/keywords/positive-feedback","display_name":"positive feedback","score":0.6259999871253967},{"id":"https://openalex.org/keywords/transcriptional-regulation","display_name":"transcriptional regulation","score":0.6510000228881836},{"id":"https://openalex.org/keywords/frameshift-mutation","display_name":"frameshift mutation","score":0.9610000252723694},{"id":"https://openalex.org/keywords/extrachromosomal-dna","display_name":"extrachromosomal DNA","score":0.8009999990463257},{"id":"https://openalex.org/keywords/self-regulation","display_name":"self-regulation","score":0.5759999752044678}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8376070261001587},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.7394446134567261},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.6686474084854126},{"id":"https://openalex.org/C179926584","wikidata":"https://www.wikidata.org/wiki/Q207714","display_name":"Transcription (linguistics)","level":2,"score":0.5725346803665161},{"id":"https://openalex.org/C207583985","wikidata":"https://www.wikidata.org/wiki/Q128723","display_name":"Wild type","level":4,"score":0.5433628559112549},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.5297378301620483},{"id":"https://openalex.org/C180754005","wikidata":"https://www.wikidata.org/wiki/Q80726","display_name":"Allele","level":3,"score":0.5283361673355103},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.5070123672485352},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4810176491737366},{"id":"https://openalex.org/C195286587","wikidata":"https://www.wikidata.org/wiki/Q424689","display_name":"Gene product","level":4,"score":0.43931224942207336},{"id":"https://openalex.org/C2780536333","wikidata":"https://www.wikidata.org/wiki/Q199456","display_name":"Paramecium","level":2,"score":0.4330882132053375},{"id":"https://openalex.org/C165864922","wikidata":"https://www.wikidata.org/wiki/Q411391","display_name":"Regulation of gene expression","level":3,"score":0.4173387289047241},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.3100995421409607},{"id":"https://openalex.org/C41895202","wikidata":"https://www.wikidata.org/wiki/Q8162","display_name":"Linguistics","level":1,"score":0.0},{"id":"https://openalex.org/C138885662","wikidata":"https://www.wikidata.org/wiki/Q5891","display_name":"Philosophy","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000953","descriptor_name":"Antigens, Protozoan","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D000953","descriptor_name":"Antigens, Protozoan","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000954","descriptor_name":"Antigens, Surface","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D000954","descriptor_name":"Antigens, Surface","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D005786","descriptor_name":"Gene Expression Regulation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013696","descriptor_name":"Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D014158","descriptor_name":"Transcription, Genetic","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015800","descriptor_name":"Protozoan Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D015800","descriptor_name":"Protozoan Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D016415","descriptor_name":"Sequence Alignment","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016805","descriptor_name":"Paramecium tetraurelia","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D016805","descriptor_name":"Paramecium tetraurelia","qualifier_ui":"Q000276","qualifier_name":"immunology","is_major_topic":false},{"descriptor_ui":"D025461","descriptor_name":"Feedback, Physiological","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1128/ec.4.10.1613-1619.2005","doi":"https://doi.org/10.1128/ec.4.10.1613-1619.2005","is_oa":true,"landing_page_url":"https://doi.org/10.1128/ec.4.10.1613-1619.2005","pdf_url":null,"source":{"id":"https://openalex.org/S177547185","display_name":"Eukaryotic Cell","issn_l":"1535-9778","issn":["1535-9778","1535-9786"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","norway-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Eukaryotic Cell","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:1265893","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/1265893","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Eukaryot Cell","raw_type":"Text"},{"id":"pmid:16215168","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/16215168","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Eukaryotic cell","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1128/ec.4.10.1613-1619.2005","doi":"https://doi.org/10.1128/ec.4.10.1613-1619.2005","is_oa":true,"landing_page_url":"https://doi.org/10.1128/ec.4.10.1613-1619.2005","pdf_url":null,"source":{"id":"https://openalex.org/S177547185","display_name":"Eukaryotic Cell","issn_l":"1535-9778","issn":["1535-9778","1535-9786"],"is_oa":true,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","jufo-1","norway-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Eukaryotic Cell","raw_type":"journal-article"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320306076","display_name":"National Science Foundation","ror":"https://ror.org/021nxhr62"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":49,"referenced_works":["https://openalex.org/W233486025","https://openalex.org/W652928918","https://openalex.org/W994306213","https://openalex.org/W1019002541","https://openalex.org/W1041627719","https://openalex.org/W1180433709","https://openalex.org/W1416589416","https://openalex.org/W1589847991","https://openalex.org/W1781520762","https://openalex.org/W1824276458","https://openalex.org/W1908384335","https://openalex.org/W1956129789","https://openalex.org/W1967921347","https://openalex.org/W1968990454","https://openalex.org/W1993220753","https://openalex.org/W2003668302","https://openalex.org/W2007150202","https://openalex.org/W2011079570","https://openalex.org/W2011571338","https://openalex.org/W2012234694","https://openalex.org/W2031358374","https://openalex.org/W2048078923","https://openalex.org/W2052693103","https://openalex.org/W2061627311","https://openalex.org/W2064440116","https://openalex.org/W2069935244","https://openalex.org/W2077476382","https://openalex.org/W2081646062","https://openalex.org/W2091609696","https://openalex.org/W2094997806","https://openalex.org/W2097952880","https://openalex.org/W2104772148","https://openalex.org/W2108730476","https://openalex.org/W2109298048","https://openalex.org/W2113324803","https://openalex.org/W2113503326","https://openalex.org/W2120564986","https://openalex.org/W2123751367","https://openalex.org/W2128846574","https://openalex.org/W2139131274","https://openalex.org/W2143418990","https://openalex.org/W2146702713","https://openalex.org/W2222752498","https://openalex.org/W2415138093","https://openalex.org/W2503957306","https://openalex.org/W3139415968","https://openalex.org/W4236446533","https://openalex.org/W4246138566","https://openalex.org/W4254689876"],"related_works":["https://openalex.org/W2415138093","https://openalex.org/W7019054623","https://openalex.org/W2157216876","https://openalex.org/W2091609696","https://openalex.org/W4236446533","https://openalex.org/W7098812109","https://openalex.org/W1589847991","https://openalex.org/W3019887471","https://openalex.org/W145694132","https://openalex.org/W7070495304"],"abstract_inverted_index":{"In":[0,75],"Paramecium":[1],"tetraurelia,":[2],"variable":[3,95],"surface":[4,90,96],"antigen":[5,25,91,97,159],"loci":[6],"show":[7],"mutually":[8],"exclusive":[9],"expression":[10,21,222],"which":[11],"is":[12,227],"controlled":[13],"primarily":[14],"at":[15,40,124,178,204,235],"the":[16,41,49,53,71,88,103,108,148,154,161,174,199,215,224],"transcriptional":[17,233],"level.":[18],"Clonally":[19],"stable":[20],"of":[22,38,147,156,173,198,214,223],"a":[23,157],"single":[24],"has":[26,44],"attracted":[27],"models":[28,131],"involving":[29],"self-regulation":[30],"by":[31],"their":[32],"gene":[33,55,92,133,226],"products.":[34],"However,":[35,135],"direct":[36],"demonstration":[37],"self-feedback":[39],"molecular":[42],"level":[43],"been":[45],"complicated":[46],"due":[47],"to":[48,51,93,188,229],"inability":[50],"separate":[52],"functional":[54],"from":[56],"its":[57,231],"product":[58],"as":[59,61,107],"well":[60],"copy":[62,105,115,213],"number":[63,106,116],"effects":[64],"associated":[65],"with":[66,129,211],"injected":[67],"extrachromosomal":[68],"DNA":[69],"in":[70,87,153,192,208],"polygenomic":[72],"somatic":[73],"nucleus.":[74],"this":[76],"study,":[77],"we":[78],"exploited":[79],"several":[80],"germ":[81],"line":[82],"termination":[83],"and":[84,111],"frameshift":[85],"mutations":[86],"A-51":[89],"analyze":[94],"expression.":[98,134],"These":[99,218],"mutant":[100,119,149,175,194,200],"alleles":[101,120],"have":[102],"same":[104],"wild-type":[109,170,216,225],"allele":[110,201],"therefore":[112],"eliminate":[113],"possible":[114],"effects.":[117],"The":[118],"were":[121],"not":[122,182],"transcribed":[123],"27":[125,189,205,236],"degrees":[126,143,190,206,237],"C,":[127],"consistent":[128],"positive-feedback":[130],"for":[132],"further":[136],"analysis":[137],"showed":[138],"that":[139,221],"high":[140,179],"temperatures":[141,180],"(34":[142],"C)":[144],"induced":[145],"transcription":[146,165,172,197],"A":[150,176],"genes":[151,177],"even":[152],"presence":[155],"different":[158],"on":[160],"cell":[162],"surface.":[163],"Thus,":[164],"was":[166,181,202],"temperature":[167,185],"dependent.":[168],"Unlike":[169],"cells,":[171],"maintained":[183,203],"after":[184],"shift":[186],"back":[187],"C":[191,207],"homozygous":[193],"cells.":[195],"Importantly,":[196],"heterozygous":[209],"cells":[210],"one":[212],"allele.":[217],"results":[219],"indicate":[220],"required":[228],"stabilize":[230],"own":[232],"state":[234],"C.":[238]},"counts_by_year":[{"year":2020,"cited_by_count":4},{"year":2015,"cited_by_count":1},{"year":2014,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}