{"id":"https://openalex.org/W2115753442","doi":"https://doi.org/10.1093/oxfordjournals.jbchem.a128648","title":"Isolation and Properties of Pig Muscle Phosphorylase*","display_name":"Isolation and Properties of Pig Muscle Phosphorylase*","publication_year":1967,"publication_date":"1967-08-01","ids":{"openalex":"https://openalex.org/W2115753442","doi":"https://doi.org/10.1093/oxfordjournals.jbchem.a128648","mag":"2115753442","pmid":"https://pubmed.ncbi.nlm.nih.gov/5586486"},"language":"en","primary_location":{"id":"doi:10.1093/oxfordjournals.jbchem.a128648","doi":"https://doi.org/10.1093/oxfordjournals.jbchem.a128648","is_oa":false,"landing_page_url":"https://doi.org/10.1093/oxfordjournals.jbchem.a128648","pdf_url":null,"source":{"id":"https://openalex.org/S184547178","display_name":"The Journal of Biochemistry","issn_l":"0021-924X","issn":["0021-924X","1756-2651"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Biochemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5035973531","display_name":"Kiyomichi Hanabusa","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I203951103","display_name":"Keio University","ror":"https://ror.org/02kn6nx58","country_code":"JP","type":"education","lineage":["https://openalex.org/I203951103"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"KIYOMICHI HANABUSA","raw_affiliation_strings":["From the Department of Biochmistry, School of Medicine, Keio University Shinjuku-ku, Tokyo"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Biochmistry, School of Medicine, Keio University Shinjuku-ku, Tokyo","institution_ids":["https://openalex.org/I203951103"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5102831723","display_name":"Takashi Kanno","orcid":"https://orcid.org/0009-0005-0488-1444","observed_orcids":["https://orcid.org/0009-0005-0488-1444"]},"institutions":[{"id":"https://openalex.org/I203951103","display_name":"Keio University","ror":"https://ror.org/02kn6nx58","country_code":"JP","type":"education","lineage":["https://openalex.org/I203951103"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"TAKASHI KANNO","raw_affiliation_strings":["From the Department of Biochmistry, School of Medicine, Keio University Shinjuku-ku, Tokyo"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Biochmistry, School of Medicine, Keio University Shinjuku-ku, Tokyo","institution_ids":["https://openalex.org/I203951103"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5022023046","display_name":"SADOA ADACHI","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I203951103","display_name":"Keio University","ror":"https://ror.org/02kn6nx58","country_code":"JP","type":"education","lineage":["https://openalex.org/I203951103"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"SADOA ADACHI","raw_affiliation_strings":["From the Department of Biochmistry, School of Medicine, Keio University Shinjuku-ku, Tokyo"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Biochmistry, School of Medicine, Keio University Shinjuku-ku, Tokyo","institution_ids":["https://openalex.org/I203951103"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5108510284","display_name":"HISAYO KOBAYASHI","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I203951103","display_name":"Keio University","ror":"https://ror.org/02kn6nx58","country_code":"JP","type":"education","lineage":["https://openalex.org/I203951103"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"HISAYO KOBAYASHI","raw_affiliation_strings":["From the Department of Biochmistry, School of Medicine, Keio University Shinjuku-ku, Tokyo"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"From the Department of Biochmistry, School of Medicine, Keio University Shinjuku-ku, Tokyo","institution_ids":["https://openalex.org/I203951103"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I203951103"],"apc_list":{"value":3276,"currency":"USD","value_usd":3276},"apc_paid":null,"fwci":0.9923,"has_fulltext":false,"cited_by_count":14,"citation_normalized_percentile":{"value":0.72000314,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":92,"max":95},"biblio":{"volume":"62","issue":"2","first_page":"194","last_page":"201"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12827","display_name":"Biochemical and Molecular Research","score":0.5702999830245972,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12827","display_name":"Biochemical and Molecular Research","score":0.5702999830245972,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.23749999701976776,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12604","display_name":"Glycogen Storage Diseases and Myoclonus","score":0.12460000067949295,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/phosphorylase-b","display_name":"phosphorylase b","score":0.7369999885559082},{"id":"https://openalex.org/keywords/phosphorylase-a","display_name":"phosphorylase a","score":0.9980000257492065},{"id":"https://openalex.org/keywords/pig-muscle","display_name":"pig muscle","score":0.8309999704360962},{"id":"https://openalex.org/keywords/enzyme-purification","display_name":"enzyme purification","score":0.9760000109672546},{"id":"https://openalex.org/keywords/enzyme-crystallization","display_name":"enzyme crystallization","score":0.859000027179718},{"id":"https://openalex.org/keywords/cysteine","display_name":"cysteine","score":0.6489999890327454},{"id":"https://openalex.org/keywords/enzymatic-polymerization","display_name":"enzymatic polymerization","score":0.6949999928474426},{"id":"https://openalex.org/keywords/sedimentation-coefficient","display_name":"sedimentation coefficient","score":0.8220000267028809},{"id":"https://openalex.org/keywords/optimal-ph","display_name":"pH optimum","score":0.8519999980926514},{"id":"https://openalex.org/keywords/pyridoxal-5-phosphate","display_name":"pyridoxal phosphate","score":0.9789999723434448},{"id":"https://openalex.org/keywords/molecular-weight","display_name":"molecular weight","score":0.4779999852180481}],"concepts":[{"id":"https://openalex.org/C204779464","wikidata":"https://www.wikidata.org/wiki/Q412061","display_name":"Glycogen phosphorylase","level":3,"score":0.8309721946716309},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.6788274049758911},{"id":"https://openalex.org/C2779201268","wikidata":"https://www.wikidata.org/wiki/Q186474","display_name":"Cysteine","level":3,"score":0.6043991446495056},{"id":"https://openalex.org/C46749446","wikidata":"https://www.wikidata.org/wiki/Q3047157","display_name":"Sedimentation equilibrium","level":3,"score":0.5555713176727295},{"id":"https://openalex.org/C2776834122","wikidata":"https://www.wikidata.org/wiki/Q418957","display_name":"Pyridoxal phosphate","level":4,"score":0.5380783677101135},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5077265501022339},{"id":"https://openalex.org/C54689828","wikidata":"https://www.wikidata.org/wiki/Q2029259","display_name":"Ultracentrifuge","level":2,"score":0.49154481291770935},{"id":"https://openalex.org/C203036418","wikidata":"https://www.wikidata.org/wiki/Q284256","display_name":"Crystallization","level":2,"score":0.4814014434814453},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.46376749873161316},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.39376240968704224},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.16117435693740845},{"id":"https://openalex.org/C197957613","wikidata":"https://www.wikidata.org/wiki/Q407280","display_name":"Cofactor","level":3,"score":0.075131356716156}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002621","descriptor_name":"Chemistry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003460","descriptor_name":"Crystallization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003545","descriptor_name":"Cysteine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004589","descriptor_name":"Electrophoresis, Disc","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005964","descriptor_name":"Glucosyltransferases","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008722","descriptor_name":"Methods","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009132","descriptor_name":"Muscles","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D013552","descriptor_name":"Swine","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014461","descriptor_name":"Ultracentrifugation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D055598","descriptor_name":"Chemical Phenomena","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1093/oxfordjournals.jbchem.a128648","doi":"https://doi.org/10.1093/oxfordjournals.jbchem.a128648","is_oa":false,"landing_page_url":"https://doi.org/10.1093/oxfordjournals.jbchem.a128648","pdf_url":null,"source":{"id":"https://openalex.org/S184547178","display_name":"The Journal of Biochemistry","issn_l":"0021-924X","issn":["0021-924X","1756-2651"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of Biochemistry","raw_type":"journal-article"},{"id":"pmid:5586486","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/5586486","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of biochemistry","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W1979219651","https://openalex.org/W1989189126","https://openalex.org/W2001108324","https://openalex.org/W1976841490","https://openalex.org/W992744832","https://openalex.org/W2402957395","https://openalex.org/W2089685340","https://openalex.org/W2096653587","https://openalex.org/W2148936883","https://openalex.org/W2056278225"],"abstract_inverted_index":{"A":[0],"method":[1],"for":[2],"the":[3,29,58,68,114,153],"purification":[4],"and":[5,26,37,95,123,127],"crystallization":[6,27],"of":[7,18,28,53,57,70,97,103,119,134,147,152],"phosphorylase":[8,21,24,65,85,142],"[EC":[9],"2.4.1.1]":[10],"b":[11,22,36,48,122],"from":[12],"pig":[13,19,120,140],"muscle":[14,20,141],"was":[15,99,105,125,137],"described.":[16],"Conversion":[17],"to":[23,64,112],"a":[25,38,42,50,66,78,86,124,131,143],"latter":[30],"form":[31],"were":[32],"accomplished.":[33],"Pig":[34],"phosphorylases":[35,121],"both":[39],"appeared":[40],"as":[41],"single":[43],"component":[44],"on":[45,107],"ultracentrifugation.":[46],"Phosphorylase":[47],"had":[49],"sedimentation":[51],"coefficient":[52],"8.5.":[54],"No":[55],"increase":[56],"S":[59],"value":[60],"occurred":[61],"upon":[62],"conversion":[63],"in":[67],"presence":[69,102],"more":[71],"than":[72,82],"0.03":[73,83],"M":[74],"cysteine,":[75],"while":[76],"at":[77],"cysteine":[79,93],"concentration":[80,94],"lower":[81],"M,":[84],"gave":[87],"heavier":[88],"components.":[89],"The":[90,101,116],"relationship":[91],"between":[92],"polymerization":[96],"molecules":[98],"discussed.":[100],"polymers":[104,110],"confirmed":[106],"disc-electrophoresis.":[108],"These":[109],"seemed":[111],"have":[113],"activity.":[115],"pH":[117],"optimum":[118],"6.4":[126],"6.2":[128],"respectively.":[129],"Assuming":[130],"molecular":[132],"weight":[133],"250,000,":[135],"it":[136],"inferred":[138],"that":[139],"contained":[144],"2":[145],"moles":[146],"pyridoxal":[148],"5\u2032-phosphate":[149],"per":[150],"mole":[151],"enzyme.":[154]},"counts_by_year":[{"year":2026,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}