{"id":"https://openalex.org/W2115657133","doi":"https://doi.org/10.1074/jbc.m703955200","title":"The HIV1 Protein Vpr Acts to Promote G2 Cell Cycle Arrest by Engaging a DDB1 and Cullin4A-containing Ubiquitin Ligase Complex Using VprBP/DCAF1 as an Adaptor","display_name":"The HIV1 Protein Vpr Acts to Promote G2 Cell Cycle Arrest by Engaging a DDB1 and Cullin4A-containing Ubiquitin Ligase Complex Using VprBP/DCAF1 as an Adaptor","publication_year":2007,"publication_date":"2007-07-09","ids":{"openalex":"https://openalex.org/W2115657133","doi":"https://doi.org/10.1074/jbc.m703955200","mag":"2115657133","pmid":"https://pubmed.ncbi.nlm.nih.gov/17620334"},"language":"en","primary_location":{"id":"doi:10.1074/jbc.m703955200","doi":"https://doi.org/10.1074/jbc.m703955200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m703955200","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"hybrid","oa_url":"https://doi.org/10.1074/jbc.m703955200","any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5100526563","display_name":"Xiaoyun Wen","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405260259","display_name":"Albany Medical College","ror":"https://ror.org/03g66yt05","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4405259726","https://openalex.org/I4405260259"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Xiaoyun Wen","raw_affiliation_strings":["Center for Immunology and Microbial Disease, Albany Medical College, Albany, New York 12208"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Immunology and Microbial Disease, Albany Medical College, Albany, New York 12208","institution_ids":["https://openalex.org/I4405260259"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5105420671","display_name":"Karen Duus","orcid":"https://orcid.org/0000-0002-1305-7458","observed_orcids":["https://orcid.org/0000-0002-1305-7458"]},"institutions":[{"id":"https://openalex.org/I4405260259","display_name":"Albany Medical College","ror":"https://ror.org/03g66yt05","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4405259726","https://openalex.org/I4405260259"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Karen M. Duus","raw_affiliation_strings":["Center for Immunology and Microbial Disease, Albany Medical College, Albany, New York 12208"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Immunology and Microbial Disease, Albany Medical College, Albany, New York 12208","institution_ids":["https://openalex.org/I4405260259"]}]},{"author_position":"middle","author":{"id":null,"display_name":"Thomas D. Friedrich","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4405260259","display_name":"Albany Medical College","ror":"https://ror.org/03g66yt05","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4405259726","https://openalex.org/I4405260259"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Thomas D. Friedrich","raw_affiliation_strings":["Center for Immunology and Microbial Disease, Albany Medical College, Albany, New York 12208"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Immunology and Microbial Disease, Albany Medical College, Albany, New York 12208","institution_ids":["https://openalex.org/I4405260259"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5072513844","display_name":"Carlos M. C. de Noronha","orcid":"https://orcid.org/0000-0001-9303-6114","observed_orcids":["https://orcid.org/0000-0001-9303-6114"]},"institutions":[{"id":"https://openalex.org/I4405260259","display_name":"Albany Medical College","ror":"https://ror.org/03g66yt05","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I4405259726","https://openalex.org/I4405260259"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"Carlos M.C. de Noronha","raw_affiliation_strings":["Center for Immunology and Microbial Disease, Albany Medical College, Albany, New York 12208. Electronic address: DeNoroC@mail.AMC.edu","Center for Immunology and Microbial Disease, Albany Medical College, Albany, New York 12208. Electronic address:"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Center for Immunology and Microbial Disease, Albany Medical College, Albany, New York 12208. Electronic address: DeNoroC@mail.AMC.edu","institution_ids":["https://openalex.org/I4405260259"]},{"raw_affiliation_string":"Center for Immunology and Microbial Disease, Albany Medical College, Albany, New York 12208. Electronic address:","institution_ids":["https://openalex.org/I4405260259"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5072513844"],"corresponding_institution_ids":["https://openalex.org/I4405260259"],"apc_list":{"value":3430,"currency":"USD","value_usd":3430},"apc_paid":{"value":3430,"currency":"USD","value_usd":3430},"fwci":5.5366,"has_fulltext":false,"cited_by_count":157,"citation_normalized_percentile":{"value":0.97098692,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":93,"max":99},"biblio":{"volume":"282","issue":"37","first_page":"27046","last_page":"27057"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10112","display_name":"HIV Research and Treatment","score":0.7900000214576721,"subfield":{"id":"https://openalex.org/subfields/2406","display_name":"Virology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10112","display_name":"HIV Research and Treatment","score":0.7900000214576721,"subfield":{"id":"https://openalex.org/subfields/2406","display_name":"Virology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11041","display_name":"Ubiquitin and proteasome pathways","score":0.1729000061750412,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10685","display_name":"Viral-associated cancers and disorders","score":0.0031999999191612005,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hiv-1-vpr","display_name":"HIV-1 Vpr","score":0.9909999966621399},{"id":"https://openalex.org/keywords/g2-cell-cycle-arrest","display_name":"G2 cell cycle arrest","score":0.8809999823570251},{"id":"https://openalex.org/keywords/ddb1","display_name":"DDB1","score":0.7300000190734863},{"id":"https://openalex.org/keywords/ubiquitin-ligase","display_name":"ubiquitin ligase","score":0.7459999918937683},{"id":"https://openalex.org/keywords/adaptor-proteins","display_name":"adaptor proteins","score":0.8299999833106995},{"id":"https://openalex.org/keywords/ubiquitination","display_name":"ubiquitination","score":0.6579999923706055},{"id":"https://openalex.org/keywords/macrophage-infection","display_name":"macrophage infection","score":0.8109999895095825},{"id":"https://openalex.org/keywords/uracil-dna-glycosylase","display_name":"uracil-DNA glycosylase","score":0.8349999785423279},{"id":"https://openalex.org/keywords/hiv-1-vif","display_name":"HIV-1 Vif","score":0.4860000014305115},{"id":"https://openalex.org/keywords/hiv-1-vpu","display_name":"HIV-1 Vpu","score":0.9990000128746033}],"concepts":[{"id":"https://openalex.org/C140248907","wikidata":"https://www.wikidata.org/wiki/Q17913449","display_name":"DDB1","level":5,"score":0.9230819940567017},{"id":"https://openalex.org/C134459356","wikidata":"https://www.wikidata.org/wiki/Q4274835","display_name":"Ubiquitin ligase","level":4,"score":0.865841269493103},{"id":"https://openalex.org/C183786373","wikidata":"https://www.wikidata.org/wiki/Q5964993","display_name":"Signal transducing adaptor protein","level":3,"score":0.7343223690986633},{"id":"https://openalex.org/C25602115","wikidata":"https://www.wikidata.org/wiki/Q407111","display_name":"Ubiquitin","level":3,"score":0.6006067991256714},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.39090174436569214},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.32853156328201294},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.24788197875022888},{"id":"https://openalex.org/C11960822","wikidata":"https://www.wikidata.org/wiki/Q242736","display_name":"Phosphorylation","level":2,"score":0.15641620755195618},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.1310887634754181},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.10149365663528442}],"mesh":[{"descriptor_ui":"D002352","descriptor_name":"Carrier Proteins","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D002478","descriptor_name":"Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004268","descriptor_name":"DNA-Binding Proteins","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016195","descriptor_name":"G2 Phase","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D016336","descriptor_name":"Gene Products, vpr","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D044767","descriptor_name":"Ubiquitin-Protein Ligases","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D044842","descriptor_name":"Cullin Proteins","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D047468","descriptor_name":"Immunoprecipitation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051981","descriptor_name":"Uracil-DNA Glycosidase","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1074/jbc.m703955200","doi":"https://doi.org/10.1074/jbc.m703955200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m703955200","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},{"id":"pmid:17620334","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/17620334","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of biological chemistry","raw_type":"Journal Article"}],"best_oa_location":{"id":"doi:10.1074/jbc.m703955200","doi":"https://doi.org/10.1074/jbc.m703955200","is_oa":true,"landing_page_url":"https://doi.org/10.1074/jbc.m703955200","pdf_url":null,"source":{"id":"https://openalex.org/S140251998","display_name":"Journal of Biological Chemistry","issn_l":"0021-9258","issn":["0021-9258","1067-8816","1083-351X"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-100","vabb-shw"],"host_organization":"https://openalex.org/P4310320990","host_organization_name":"Elsevier BV","host_organization_lineage":["https://openalex.org/P4310320990"],"host_organization_lineage_names":["Elsevier BV"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Biological Chemistry","raw_type":"journal-article"},"sustainable_development_goals":[{"id":"https://metadata.un.org/sdg/3","score":0.8048999905586243,"display_name":"Good health and well-being"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G7029553825","display_name":"Vpr as a Mediator Proteasomal Degradation","funder_award_id":"R01AI073178","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"}],"funders":[{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":57,"referenced_works":["https://openalex.org/W1480448067","https://openalex.org/W1544820414","https://openalex.org/W1557691749","https://openalex.org/W1714042500","https://openalex.org/W1807747199","https://openalex.org/W1961313548","https://openalex.org/W1978560543","https://openalex.org/W1982537658","https://openalex.org/W1985585046","https://openalex.org/W1985903968","https://openalex.org/W1992602932","https://openalex.org/W1993611139","https://openalex.org/W1998954137","https://openalex.org/W2001243226","https://openalex.org/W2005973634","https://openalex.org/W2007320083","https://openalex.org/W2010840723","https://openalex.org/W2023668959","https://openalex.org/W2024569169","https://openalex.org/W2026218505","https://openalex.org/W2028440304","https://openalex.org/W2034626290","https://openalex.org/W2041676007","https://openalex.org/W2047028501","https://openalex.org/W2048656467","https://openalex.org/W2052406246","https://openalex.org/W2053332066","https://openalex.org/W2061941585","https://openalex.org/W2065364467","https://openalex.org/W2066071859","https://openalex.org/W2066280128","https://openalex.org/W2068784898","https://openalex.org/W2072901265","https://openalex.org/W2074110630","https://openalex.org/W2089135460","https://openalex.org/W2094023425","https://openalex.org/W2095910839","https://openalex.org/W2097744772","https://openalex.org/W2101969937","https://openalex.org/W2103723894","https://openalex.org/W2122266844","https://openalex.org/W2123911636","https://openalex.org/W2124389350","https://openalex.org/W2129784359","https://openalex.org/W2132256977","https://openalex.org/W2140226886","https://openalex.org/W2143261659","https://openalex.org/W2145844721","https://openalex.org/W2147211953","https://openalex.org/W2148634265","https://openalex.org/W2149678150","https://openalex.org/W2157111385","https://openalex.org/W2159113944","https://openalex.org/W2166756978","https://openalex.org/W2170434135","https://openalex.org/W2412751133","https://openalex.org/W6990086693"],"related_works":["https://openalex.org/W2026218505","https://openalex.org/W2010577903","https://openalex.org/W2088542873","https://openalex.org/W1965565827","https://openalex.org/W7100648234","https://openalex.org/W2119654660","https://openalex.org/W2146538416","https://openalex.org/W1982434350","https://openalex.org/W1999059316","https://openalex.org/W6977104461"],"abstract_inverted_index":{"The":[0],"roles":[1],"of":[2,15,32,94,113],"the":[3,91,100,105,114,131,152],"HIV1":[4,56,153],"protein":[5],"Vpr":[6,16,26,57,68,73,103,129],"in":[7,17,24,127],"virus":[8],"replication":[9],"and":[10,40,49,61,74,80,104,156],"pathogenesis":[11],"remain":[12],"unclear.":[13],"Expression":[14],"dividing":[18],"cells":[19],"causes":[20],"cell":[21,143],"cycle":[22,144],"arrest":[23,79],"G(2).":[25],"also":[27],"facilitates":[28],"low":[29],"titer":[30],"infection":[31],"terminally":[33],"differentiated":[34],"macrophages,":[35],"enhances":[36],"transcription,":[37],"promotes":[38],"apoptosis,":[39],"targets":[41],"cellular":[42,137,160],"uracil":[43],"N-glycosylase":[44],"for":[45,118,142,163],"degradation.":[46],"Using":[47],"co-immunoprecipitation":[48],"tandem":[50],"mass":[51],"spectroscopy,":[52],"we":[53],"found":[54],"that":[55,99,139],"engages":[58,90,130],"a":[59,125,136],"DDB1-":[60],"cullin4A-containing":[62],"ubiquitin-ligase":[63,106,115],"complex":[64],"through":[65],"VprBP/DCAF1.":[66],"HIV2":[67,85],"has":[69],"two":[70],"Vpr-like":[71],"proteins,":[72],"Vpx,":[75,89],"which":[76,128],"cause":[77],"G(2)":[78,120],"facilitate":[81],"macrophage":[82],"infection,":[83],"respectively.":[84],"Vpr,":[86,148],"but":[87],"not":[88],"same":[92],"set":[93],"proteins.":[95],"We":[96],"further":[97,111],"demonstrate":[98],"interaction":[101],"between":[102],"components":[107],"as":[108,110],"well":[109],"assembly":[112],"are":[116],"necessary":[117],"Vpr-mediated":[119],"arrest.":[121],"Our":[122],"data":[123],"support":[124],"model":[126],"ubiquitin":[132,161],"ligase":[133],"to":[134,158],"deplete":[135],"factor":[138],"is":[140],"required":[141],"progression":[145],"into":[146],"mitosis.":[147],"thus,":[149],"functions":[150],"like":[151],"proteins":[154],"Vif":[155],"Vpu":[157],"usurp":[159],"ligases":[162],"viral":[164],"functions.":[165]},"counts_by_year":[{"year":2026,"cited_by_count":6},{"year":2025,"cited_by_count":2},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":5},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":7},{"year":2020,"cited_by_count":4},{"year":2019,"cited_by_count":5},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":6},{"year":2016,"cited_by_count":9},{"year":2015,"cited_by_count":8},{"year":2014,"cited_by_count":13},{"year":2013,"cited_by_count":6},{"year":2012,"cited_by_count":14}],"updated_date":"2026-11-13T13:42:25.865203","created_date":"2016-06-24T00:00:00"}
