{"id":"https://openalex.org/W2114954091","doi":"https://doi.org/10.2144/97221rr03","title":"Tracking and Quantitation of Retroviral-Mediated Transfer Using a Completely Humanized, Red-Shifted Green Fluorescent Protein Gene","display_name":"Tracking and Quantitation of Retroviral-Mediated Transfer Using a Completely Humanized, Red-Shifted Green Fluorescent Protein Gene","publication_year":1997,"publication_date":"1997-01-01","ids":{"openalex":"https://openalex.org/W2114954091","doi":"https://doi.org/10.2144/97221rr03","mag":"2114954091","pmid":"https://pubmed.ncbi.nlm.nih.gov/8994663"},"language":"en","primary_location":{"id":"doi:10.2144/97221rr03","doi":"https://doi.org/10.2144/97221rr03","is_oa":true,"landing_page_url":"https://doi.org/10.2144/97221rr03","pdf_url":null,"source":{"id":"https://openalex.org/S79990748","display_name":"BioTechniques","issn_l":"0736-6205","issn":["0736-6205","1940-9818"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"gold","oa_url":"https://doi.org/10.2144/97221rr03","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5042744604","display_name":"Rebecca R. Muldoon","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210131176","display_name":"Takara (United States)","ror":"https://ror.org/02js6ft04","country_code":"US","type":"company","lineage":["https://openalex.org/I4210113000","https://openalex.org/I4210131176"]},{"id":"https://openalex.org/I4210147210","display_name":"Human Gene Therapy Research Institute","ror":"https://ror.org/04dfe6667","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I4210147210"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"R.R. Muldoon","raw_affiliation_strings":["CLONTECH Laboratories, Palo Alto, CA, USA","Human Gene Therapy Research Institute, Central Iowa Health Systems, Des Moines, IA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"CLONTECH Laboratories, Palo Alto, CA, USA","institution_ids":["https://openalex.org/I4210131176"]},{"raw_affiliation_string":"Human Gene Therapy Research Institute, Central Iowa Health Systems, Des Moines, IA","institution_ids":["https://openalex.org/I4210147210"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5109937175","display_name":"John P. Levy","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210131176","display_name":"Takara (United States)","ror":"https://ror.org/02js6ft04","country_code":"US","type":"company","lineage":["https://openalex.org/I4210113000","https://openalex.org/I4210131176"]},{"id":"https://openalex.org/I4210147210","display_name":"Human Gene Therapy Research Institute","ror":"https://ror.org/04dfe6667","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I4210147210"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"J.P. Levy","raw_affiliation_strings":["CLONTECH Laboratories, Palo Alto, CA, USA","Human Gene Therapy Research Institute, Central Iowa Health Systems, Des Moines, IA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"CLONTECH Laboratories, Palo Alto, CA, USA","institution_ids":["https://openalex.org/I4210131176"]},{"raw_affiliation_string":"Human Gene Therapy Research Institute, Central Iowa Health Systems, Des Moines, IA","institution_ids":["https://openalex.org/I4210147210"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5108540856","display_name":"Steven R. Kain","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210131176","display_name":"Takara (United States)","ror":"https://ror.org/02js6ft04","country_code":"US","type":"company","lineage":["https://openalex.org/I4210113000","https://openalex.org/I4210131176"]},{"id":"https://openalex.org/I4210147210","display_name":"Human Gene Therapy Research Institute","ror":"https://ror.org/04dfe6667","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I4210147210"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"S.R. Kain","raw_affiliation_strings":["CLONTECH Laboratories, Palo Alto, CA, USA","Human Gene Therapy Research Institute, Central Iowa Health Systems, Des Moines, IA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"CLONTECH Laboratories, Palo Alto, CA, USA","institution_ids":["https://openalex.org/I4210131176"]},{"raw_affiliation_string":"Human Gene Therapy Research Institute, Central Iowa Health Systems, Des Moines, IA","institution_ids":["https://openalex.org/I4210147210"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5111417138","display_name":"P. A. Kitts","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210131176","display_name":"Takara (United States)","ror":"https://ror.org/02js6ft04","country_code":"US","type":"company","lineage":["https://openalex.org/I4210113000","https://openalex.org/I4210131176"]},{"id":"https://openalex.org/I4210147210","display_name":"Human Gene Therapy Research Institute","ror":"https://ror.org/04dfe6667","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I4210147210"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"P.A. Kitts","raw_affiliation_strings":["CLONTECH Laboratories, Palo Alto, CA, USA","Human Gene Therapy Research Institute, Central Iowa Health Systems, Des Moines, IA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"CLONTECH Laboratories, Palo Alto, CA, USA","institution_ids":["https://openalex.org/I4210131176"]},{"raw_affiliation_string":"Human Gene Therapy Research Institute, Central Iowa Health Systems, Des Moines, IA","institution_ids":["https://openalex.org/I4210147210"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5112243123","display_name":"Charles J. Link","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210147210","display_name":"Human Gene Therapy Research Institute","ror":"https://ror.org/04dfe6667","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I4210147210"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"C.J. Link","raw_affiliation_strings":["Human Gene Therapy Research Institute, Central Iowa Health Systems, Des Moines, IA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Human Gene Therapy Research Institute, Central Iowa Health Systems, Des Moines, IA","institution_ids":["https://openalex.org/I4210147210"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":1415,"currency":"USD","value_usd":1415},"apc_paid":{"value":1415,"currency":"USD","value_usd":1415},"fwci":3.8405,"has_fulltext":false,"cited_by_count":41,"citation_normalized_percentile":{"value":0.94192855,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"22","issue":"1","first_page":"162","last_page":"167"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10613","display_name":"Virus-based gene therapy research","score":0.9976000189781189,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10725","display_name":"RNA Interference and Gene Delivery","score":0.0005000000237487257,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10691","display_name":"Retinal Development and Disorders","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2731","display_name":"Ophthalmology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/retroviral-vectors","display_name":"retroviral vectors","score":0.8629999756813049},{"id":"https://openalex.org/keywords/retroviral-gene-transfer","display_name":"retroviral gene transfer","score":0.7919999957084656},{"id":"https://openalex.org/keywords/melanoma-cells","display_name":"melanoma cells","score":0.7260000109672546},{"id":"https://openalex.org/keywords/ovarian-cancer-cells","display_name":"ovarian cancer cells","score":0.9919999837875366},{"id":"https://openalex.org/keywords/in-vivo-gene-expression","display_name":"in vivo gene expression","score":0.5320000052452087}],"concepts":[{"id":"https://openalex.org/C142613039","wikidata":"https://www.wikidata.org/wiki/Q272983","display_name":"Green fluorescent protein","level":3,"score":0.8907284140586853},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6991723775863647},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.6693616509437561},{"id":"https://openalex.org/C54009773","wikidata":"https://www.wikidata.org/wiki/Q1429031","display_name":"Transfection","level":3,"score":0.640570878982544},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.6262328624725342},{"id":"https://openalex.org/C111599444","wikidata":"https://www.wikidata.org/wiki/Q213901","display_name":"Genetic enhancement","level":3,"score":0.5613552331924438},{"id":"https://openalex.org/C150194340","wikidata":"https://www.wikidata.org/wiki/Q26972","display_name":"Gene expression","level":3,"score":0.5503357648849487},{"id":"https://openalex.org/C81885089","wikidata":"https://www.wikidata.org/wiki/Q189082","display_name":"Cell culture","level":2,"score":0.549502968788147},{"id":"https://openalex.org/C22744801","wikidata":"https://www.wikidata.org/wiki/Q172778","display_name":"Plasmid","level":3,"score":0.508449912071228},{"id":"https://openalex.org/C202751555","wikidata":"https://www.wikidata.org/wiki/Q221681","display_name":"In vitro","level":2,"score":0.49253425002098083},{"id":"https://openalex.org/C175656101","wikidata":"https://www.wikidata.org/wiki/Q56646","display_name":"Chinese hamster ovary cell","level":3,"score":0.47234049439430237},{"id":"https://openalex.org/C32470452","wikidata":"https://www.wikidata.org/wiki/Q2298489","display_name":"Viral vector","level":4,"score":0.47104620933532715},{"id":"https://openalex.org/C3018928802","wikidata":"https://www.wikidata.org/wiki/Q83185","display_name":"Gene transfer","level":3,"score":0.42441526055336},{"id":"https://openalex.org/C207001950","wikidata":"https://www.wikidata.org/wiki/Q141124","display_name":"In vivo","level":2,"score":0.4138592779636383},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.28632280230522156},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.22917044162750244}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003001","descriptor_name":"Cloning, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005434","descriptor_name":"Flow Cytometry","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005819","descriptor_name":"Genetic Markers","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005822","descriptor_name":"Genetic Vectors","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008164","descriptor_name":"Luminescent Proteins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D008164","descriptor_name":"Luminescent Proteins","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D012190","descriptor_name":"Retroviridae","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D014162","descriptor_name":"Transfection","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014407","descriptor_name":"Tumor Cells, Cultured","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015870","descriptor_name":"Gene Expression","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D018014","descriptor_name":"Gene Transfer Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D037401","descriptor_name":"Scyphozoa","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D037401","descriptor_name":"Scyphozoa","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.2144/97221rr03","doi":"https://doi.org/10.2144/97221rr03","is_oa":true,"landing_page_url":"https://doi.org/10.2144/97221rr03","pdf_url":null,"source":{"id":"https://openalex.org/S79990748","display_name":"BioTechniques","issn_l":"0736-6205","issn":["0736-6205","1940-9818"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"journal-article"},{"id":"pmid:8994663","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/8994663","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"Journal Article"},{"id":"pmh:oai:doaj.org/article:e00edd3a0dfa414ea4f2f428f163e29e","doi":null,"is_oa":true,"landing_page_url":"https://doaj.org/article/e00edd3a0dfa414ea4f2f428f163e29e","pdf_url":null,"source":{"id":"https://openalex.org/S4306401280","display_name":"DOAJ (DOAJ: Directory of Open Access Journals)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-sa","license_id":"https://openalex.org/licenses/cc-by-sa","version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"BioTechniques, Vol 22, Iss 1, Pp 162-167 (1997)","raw_type":"article"}],"best_oa_location":{"id":"doi:10.2144/97221rr03","doi":"https://doi.org/10.2144/97221rr03","is_oa":true,"landing_page_url":"https://doi.org/10.2144/97221rr03","pdf_url":null,"source":{"id":"https://openalex.org/S79990748","display_name":"BioTechniques","issn_l":"0736-6205","issn":["0736-6205","1940-9818"],"is_oa":true,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-c","jufo-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320316","host_organization_name":"Future Science Ltd","host_organization_lineage":["https://openalex.org/P4310320316"],"host_organization_lineage_names":["Future Science Ltd"],"type":"journal"},"license":"cc-by","license_id":"https://openalex.org/licenses/cc-by","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"BioTechniques","raw_type":"journal-article"},"sustainable_development_goals":[{"score":0.504800021648407,"id":"https://metadata.un.org/sdg/3","display_name":"Good health and well-being"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":12,"referenced_works":["https://openalex.org/W1490724066","https://openalex.org/W1980073547","https://openalex.org/W2003306608","https://openalex.org/W2014293907","https://openalex.org/W2025796534","https://openalex.org/W2062230922","https://openalex.org/W2071441657","https://openalex.org/W2086991026","https://openalex.org/W2087823077","https://openalex.org/W2110310164","https://openalex.org/W2131974188","https://openalex.org/W2161545291"],"related_works":["https://openalex.org/W1994740410","https://openalex.org/W155385898","https://openalex.org/W2366736383","https://openalex.org/W1981058262","https://openalex.org/W1482763533","https://openalex.org/W2161718684","https://openalex.org/W2054131802","https://openalex.org/W28588605","https://openalex.org/W2064893019","https://openalex.org/W2292300172"],"abstract_inverted_index":{"We":[0,25,48],"have":[1],"developed":[2],"murine":[3],"retroviral":[4,17],"vectors":[5],"(RVs)":[6],"containing":[7],"an":[8],"optimized":[9],"green":[10],"fluorescent":[11],"protein":[12],"(GFP)":[13],"gene":[14,18,52,107,117],"to":[15,115],"study":[16,116],"transfer":[19],"and":[20,86,120,125],"expression":[21,101,119],"in":[22,75,108,123,126],"living":[23],"cells.":[24,81],"used":[26],"the":[27,40,44,50,54,80],"codon":[28],"\"humanized\",":[29],"\"red-shifted\"":[30],"GFP":[31,42],"gene,":[32],"hGFP-S65T,":[33],"a":[34,103,112],"gain":[35],"of":[36,39,79,98,102],"function":[37],"variant":[38],"wild-type":[41],"from":[43,92],"jellyfish":[45],"Aequorea":[46],"victoria.":[47],"cloned":[49],"hGFP-S65T":[51,106],"into":[53],"RV":[55],"plasmid":[56],"pLNCX":[57],"(pLNChG65T).":[58],"A":[59],"stable":[60],"amphotropic":[61],"RV-producer":[62],"cell":[63],"line":[64],"(VPC),":[65],"designated":[66],"LNChG65T":[67],"VPC,":[68],"was":[69],"generated":[70],"that":[71],"exhibited":[72],"bright":[73],"fluorescence":[74],"greater":[76],"than":[77],"95%":[78],"Human":[82],"A375":[83],"melanoma":[84],"cells":[85,90,110],"IGROV":[87],"ovarian":[88],"carcinoma":[89],"transduced":[91],"LNCh-G65T":[93],"VPC":[94],"demonstrated":[95],"high":[96],"levels":[97],"fluorescence.":[99],"The":[100],"single":[104],"integrated":[105],"eukaryotic":[109],"provides":[111],"powerful":[113],"tool":[114],"transfer,":[118],"functional":[121],"studies":[122],"vitro":[124],"vivo.":[127]},"counts_by_year":[{"year":2019,"cited_by_count":1},{"year":2016,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}