{"id":"https://openalex.org/W2114220091","doi":"https://doi.org/10.1002/cbic.201402677","title":"Novel Approaches in Selective Tryptophan Isotope Labeling by Using Escherichia coli Overexpression Media","display_name":"Novel Approaches in Selective Tryptophan Isotope Labeling by Using Escherichia coli Overexpression Media","publication_year":2015,"publication_date":"2015-02-20","ids":{"openalex":"https://openalex.org/W2114220091","doi":"https://doi.org/10.1002/cbic.201402677","mag":"2114220091","pmid":"https://pubmed.ncbi.nlm.nih.gov/25703586"},"language":"en","primary_location":{"id":"doi:10.1002/cbic.201402677","doi":"https://doi.org/10.1002/cbic.201402677","is_oa":false,"landing_page_url":"https://doi.org/10.1002/cbic.201402677","pdf_url":null,"source":{"id":"https://openalex.org/S154285657","display_name":"ChemBioChem","issn_l":"1439-4227","issn":["1439-4227","1439-7633"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ChemBioChem","raw_type":"journal-article"},"parent_works":[],"lineage":["https://openalex.org/W2114220091"],"child_works_count":0,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5055733523","display_name":"Julia Sch\u00f6rghuber","orcid":"https://orcid.org/0000-0002-9409-281X","observed_orcids":["https://orcid.org/0000-0002-9409-281X"]},"institutions":[{"id":"https://openalex.org/I129774422","display_name":"University of Vienna","ror":"https://ror.org/03prydq77","country_code":"AT","type":"education","lineage":["https://openalex.org/I129774422"]}],"countries":["AT"],"is_corresponding":false,"raw_author_name":"Julia Sch\u00f6rghuber","raw_affiliation_strings":["Institute of Organic Chemistry, University of Vienna, W\u00e4hringerstrasse 38, 1090 Vienna (Austria)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Organic Chemistry, University of Vienna, W\u00e4hringerstrasse 38, 1090 Vienna (Austria)","institution_ids":["https://openalex.org/I129774422"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5078688672","display_name":"Tom\u00e1\u0161 S\u00e1ra","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I129774422","display_name":"University of Vienna","ror":"https://ror.org/03prydq77","country_code":"AT","type":"education","lineage":["https://openalex.org/I129774422"]},{"id":"https://openalex.org/I2802916955","display_name":"Max Perutz Labs","ror":"https://ror.org/05cz70a34","country_code":"AT","type":"facility","lineage":["https://openalex.org/I129774422","https://openalex.org/I2802916955"]}],"countries":["AT"],"is_corresponding":false,"raw_author_name":"Tom\u00e1\u0161 S\u00e1ra","raw_affiliation_strings":["Department of Structural and Computational Biology, Max F. Perutz Laboratories, University of Vienna, Dr. Bohr-Gasse 9, 1030 Vienna (Austria)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Structural and Computational Biology, Max F. Perutz Laboratories, University of Vienna, Dr. Bohr-Gasse 9, 1030 Vienna (Austria)","institution_ids":["https://openalex.org/I129774422","https://openalex.org/I2802916955"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5045281289","display_name":"Marilena Bisaccia","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I129774422","display_name":"University of Vienna","ror":"https://ror.org/03prydq77","country_code":"AT","type":"education","lineage":["https://openalex.org/I129774422"]}],"countries":["AT"],"is_corresponding":false,"raw_author_name":"Marilena Bisaccia","raw_affiliation_strings":["Institute of Organic Chemistry, University of Vienna, Wa\u0308hringerstrasse 38, 1090 Vienna (Austria)","Institute of Organic Chemistry, University of Vienna, W\u00e4hringerstrasse 38, 1090 Vienna (Austria)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Organic Chemistry, University of Vienna, Wa\u0308hringerstrasse 38, 1090 Vienna (Austria)","institution_ids":["https://openalex.org/I129774422"]},{"raw_affiliation_string":"Institute of Organic Chemistry, University of Vienna, W\u00e4hringerstrasse 38, 1090 Vienna (Austria)","institution_ids":["https://openalex.org/I129774422"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5110136013","display_name":"Walther Schmid","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I129774422","display_name":"University of Vienna","ror":"https://ror.org/03prydq77","country_code":"AT","type":"education","lineage":["https://openalex.org/I129774422"]}],"countries":["AT"],"is_corresponding":false,"raw_author_name":"Walther Schmid","raw_affiliation_strings":["Institute of Organic Chemistry, University of Vienna, Wa\u0308hringerstrasse 38, 1090 Vienna (Austria)","Institute of Organic Chemistry, University of Vienna, W\u00e4hringerstrasse 38, 1090 Vienna (Austria)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Organic Chemistry, University of Vienna, Wa\u0308hringerstrasse 38, 1090 Vienna (Austria)","institution_ids":["https://openalex.org/I129774422"]},{"raw_affiliation_string":"Institute of Organic Chemistry, University of Vienna, W\u00e4hringerstrasse 38, 1090 Vienna (Austria)","institution_ids":["https://openalex.org/I129774422"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5077702058","display_name":"Robert Konrat","orcid":"https://orcid.org/0000-0001-6489-4080","observed_orcids":["https://orcid.org/0000-0001-6489-4080"]},"institutions":[{"id":"https://openalex.org/I129774422","display_name":"University of Vienna","ror":"https://ror.org/03prydq77","country_code":"AT","type":"education","lineage":["https://openalex.org/I129774422"]},{"id":"https://openalex.org/I2802916955","display_name":"Max Perutz Labs","ror":"https://ror.org/05cz70a34","country_code":"AT","type":"facility","lineage":["https://openalex.org/I129774422","https://openalex.org/I2802916955"]}],"countries":["AT"],"is_corresponding":false,"raw_author_name":"Robert Konrat","raw_affiliation_strings":["Department of Structural and Computational Biology, Max F. Perutz Laboratories, University of Vienna, Dr. Bohr-Gasse 9, 1030 Vienna (Austria)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Structural and Computational Biology, Max F. Perutz Laboratories, University of Vienna, Dr. Bohr-Gasse 9, 1030 Vienna (Austria)","institution_ids":["https://openalex.org/I129774422","https://openalex.org/I2802916955"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5027297889","display_name":"Roman J. Lichtenecker","orcid":"https://orcid.org/0000-0003-2168-5216","observed_orcids":["https://orcid.org/0000-0003-2168-5216"]},"institutions":[{"id":"https://openalex.org/I129774422","display_name":"University of Vienna","ror":"https://ror.org/03prydq77","country_code":"AT","type":"education","lineage":["https://openalex.org/I129774422"]}],"countries":["AT"],"is_corresponding":true,"raw_author_name":"Roman J. Lichtenecker","raw_affiliation_strings":["Institute of Organic Chemistry, University of Vienna, Wa\u0308hringerstrasse 38, 1090 Vienna (Austria)","Institute of Organic Chemistry, University of Vienna, W\u00e4hringerstrasse 38, 1090 Vienna (Austria)"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Organic Chemistry, University of Vienna, Wa\u0308hringerstrasse 38, 1090 Vienna (Austria)","institution_ids":["https://openalex.org/I129774422"]},{"raw_affiliation_string":"Institute of Organic Chemistry, University of Vienna, W\u00e4hringerstrasse 38, 1090 Vienna (Austria)","institution_ids":["https://openalex.org/I129774422"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":["https://openalex.org/A5027297889"],"corresponding_institution_ids":["https://openalex.org/I129774422"],"apc_list":{"value":4430,"currency":"USD","value_usd":4430},"apc_paid":null,"fwci":1.5558,"has_fulltext":false,"cited_by_count":25,"citation_normalized_percentile":{"value":0.78358897,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":99},"biblio":{"volume":"16","issue":"5","first_page":"746","last_page":"751"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11809","display_name":"Advanced NMR Techniques and Applications","score":0.9887999892234802,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11809","display_name":"Advanced NMR Techniques and Applications","score":0.9887999892234802,"subfield":{"id":"https://openalex.org/subfields/1607","display_name":"Spectroscopy"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.005799999926239252,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10836","display_name":"Metabolomics and Mass Spectrometry Studies","score":0.0007999999797903001,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/protein-nmr-spectroscopy","display_name":"protein NMR","score":0.7049999833106995},{"id":"https://openalex.org/keywords/protein-conformational-change","display_name":"protein conformational change","score":0.6990000009536743},{"id":"https://openalex.org/keywords/protein-protein-interaction","display_name":"protein-protein interaction","score":0.6970000267028809},{"id":"https://openalex.org/keywords/small-molecule-binding","display_name":"small molecule binding","score":0.578000009059906}],"concepts":[{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.8441712856292725},{"id":"https://openalex.org/C2776706248","wikidata":"https://www.wikidata.org/wiki/Q181003","display_name":"Tryptophan","level":3,"score":0.687870442867279},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5745038986206055},{"id":"https://openalex.org/C164304813","wikidata":"https://www.wikidata.org/wiki/Q25276","display_name":"Isotope","level":2,"score":0.4940052628517151},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.38108909130096436},{"id":"https://openalex.org/C70721500","wikidata":"https://www.wikidata.org/wiki/Q177005","display_name":"Computational biology","level":1,"score":0.3558223247528076},{"id":"https://openalex.org/C21951064","wikidata":"https://www.wikidata.org/wiki/Q899212","display_name":"Combinatorial chemistry","level":1,"score":0.3263322412967682},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.29184240102767944},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.20455598831176758},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.13694098591804504},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.10663929581642151},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D002247","descriptor_name":"Carbon Isotopes","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003903","descriptor_name":"Deuterium","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D007211","descriptor_name":"Indoles","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D007211","descriptor_name":"Indoles","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D007553","descriptor_name":"Isotope Labeling","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D011522","descriptor_name":"Protons","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014364","descriptor_name":"Tryptophan","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014364","descriptor_name":"Tryptophan","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D015394","descriptor_name":"Molecular Structure","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019906","descriptor_name":"Nuclear Magnetic Resonance, Biomolecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1002/cbic.201402677","doi":"https://doi.org/10.1002/cbic.201402677","is_oa":false,"landing_page_url":"https://doi.org/10.1002/cbic.201402677","pdf_url":null,"source":{"id":"https://openalex.org/S154285657","display_name":"ChemBioChem","issn_l":"1439-4227","issn":["1439-4227","1439-7633"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2","vabb-shw"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"ChemBioChem","raw_type":"journal-article"},{"id":"pmid:25703586","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/25703586","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Chembiochem : a European journal of chemical biology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":54,"referenced_works":["https://openalex.org/W384364580","https://openalex.org/W1557897326","https://openalex.org/W1596745037","https://openalex.org/W1965826248","https://openalex.org/W1968533552","https://openalex.org/W1968932730","https://openalex.org/W1970433872","https://openalex.org/W1972403855","https://openalex.org/W1983099248","https://openalex.org/W1983614185","https://openalex.org/W1983669981","https://openalex.org/W1988012381","https://openalex.org/W1989045942","https://openalex.org/W1989166139","https://openalex.org/W1989480045","https://openalex.org/W1990032822","https://openalex.org/W1990221427","https://openalex.org/W1992577725","https://openalex.org/W1998491244","https://openalex.org/W1999480103","https://openalex.org/W2001671479","https://openalex.org/W2006863129","https://openalex.org/W2022896304","https://openalex.org/W2024079434","https://openalex.org/W2024419304","https://openalex.org/W2026423416","https://openalex.org/W2029656409","https://openalex.org/W2033975503","https://openalex.org/W2042470929","https://openalex.org/W2051710717","https://openalex.org/W2052769471","https://openalex.org/W2056372211","https://openalex.org/W2058539400","https://openalex.org/W2061340662","https://openalex.org/W2070915371","https://openalex.org/W2071556057","https://openalex.org/W2072796170","https://openalex.org/W2078892740","https://openalex.org/W2087285581","https://openalex.org/W2092285339","https://openalex.org/W2104224990","https://openalex.org/W2105776901","https://openalex.org/W2106135117","https://openalex.org/W2111456228","https://openalex.org/W2113670799","https://openalex.org/W2124185101","https://openalex.org/W2134358906","https://openalex.org/W2136206140","https://openalex.org/W2138425518","https://openalex.org/W2150150853","https://openalex.org/W2158060231","https://openalex.org/W2158105833","https://openalex.org/W2159307624","https://openalex.org/W2315167156"],"related_works":["https://openalex.org/W2751759701","https://openalex.org/W2612416649","https://openalex.org/W3213296047","https://openalex.org/W1999480103","https://openalex.org/W2024690544","https://openalex.org/W2607179568","https://openalex.org/W3197047708","https://openalex.org/W4404050144","https://openalex.org/W2033975503","https://openalex.org/W6928188578"],"abstract_inverted_index":{"NMR-based":[0],"investigations":[1],"of":[2,28,60,78],"large":[3],"protein":[4,31],"complexes":[5],"require":[6],"optimized":[7],"isotopic":[8],"labeling":[9,36],"schemes.":[10],"We":[11],"report":[12],"new":[13,42],"methods":[14],"to":[15,25,57,94],"introduce":[16],"stable":[17],"isotopes":[18],"into":[19],"tryptophan":[20,90],"residues;":[21],"these":[22,79],"are":[23],"fine-tuned":[24],"the":[26,29,76],"requirements":[27],"particular":[30],"NMR":[32],"experiment.":[33],"Selective":[34],"backbone":[35],"was":[37],"performed":[38],"by":[39,67],"using":[40],"a":[41],"\u03b1-ketoacid":[43],"precursor":[44,80],"as":[45,98],"an":[46],"additive":[47],"in":[48,75,89,92],"cell-based":[49],"overexpression":[50],"media.":[51],"Additionally,":[52],"we":[53],"developed":[54],"synthetic":[55],"routes":[56],"certain":[58],"isotopologues":[59],"indole":[61],"with":[62,100],"(13)C-(1)H":[63],"spin":[64],"systems":[65],"surrounded":[66],"(12)C":[68],"and":[69],"(2)H.":[70],"The":[71],"corresponding":[72],"proteins,":[73],"overexpressed":[74],"presence":[77],"compounds,":[81],"can":[82],"be":[83],"effectively":[84],"analyzed":[85],"for":[86],"conformational":[87],"changes":[88],"residues":[91],"response":[93],"external":[95],"stimuli,":[96],"such":[97],"interaction":[99],"other":[101],"proteins":[102],"or":[103],"small":[104],"molecules.":[105]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2024,"cited_by_count":5},{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":2},{"year":2019,"cited_by_count":1},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":8},{"year":2016,"cited_by_count":2}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}