{"id":"https://openalex.org/W2113647940","doi":"https://doi.org/10.1128/jvi.78.1.320-328.2004","title":"The ORF2 Protein of Hepatitis E Virus Binds the 5\u2032 Region of Viral RNA","display_name":"The ORF2 Protein of Hepatitis E Virus Binds the 5\u2032 Region of Viral RNA","publication_year":2003,"publication_date":"2003-12-11","ids":{"openalex":"https://openalex.org/W2113647940","doi":"https://doi.org/10.1128/jvi.78.1.320-328.2004","mag":"2113647940","pmid":"https://pubmed.ncbi.nlm.nih.gov/14671114","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/303377"},"language":"en","primary_location":{"id":"doi:10.1128/jvi.78.1.320-328.2004","doi":"https://doi.org/10.1128/jvi.78.1.320-328.2004","is_oa":false,"landing_page_url":"https://doi.org/10.1128/jvi.78.1.320-328.2004","pdf_url":null,"source":{"id":"https://openalex.org/S96615428","display_name":"Journal of Virology","issn_l":"0022-538X","issn":["0022-538X","1070-6321","1098-5514"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Virology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/303377","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5022170940","display_name":"Milan Surjit","orcid":"https://orcid.org/0000-0001-8435-3257","observed_orcids":["https://orcid.org/0000-0001-8435-3257"]},"institutions":[{"id":"https://openalex.org/I4210142124","display_name":"International Centre for Genetic Engineering and Biotechnology","ror":"https://ror.org/03j4rrt43","country_code":"IN","type":"facility","lineage":["https://openalex.org/I4210142124","https://openalex.org/I91080651"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Milan Surjit","raw_affiliation_strings":["Virology Group, International Centre for Genetic Engineering & Biotechnology, New Delhi 110067, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Virology Group, International Centre for Genetic Engineering & Biotechnology, New Delhi 110067, India","institution_ids":["https://openalex.org/I4210142124"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5076248093","display_name":"Shahid Jameel","orcid":"https://orcid.org/0000-0001-5424-9774","observed_orcids":["https://orcid.org/0000-0001-5424-9774"]},"institutions":[{"id":"https://openalex.org/I4210142124","display_name":"International Centre for Genetic Engineering and Biotechnology","ror":"https://ror.org/03j4rrt43","country_code":"IN","type":"facility","lineage":["https://openalex.org/I4210142124","https://openalex.org/I91080651"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Shahid Jameel","raw_affiliation_strings":["Virology Group, International Centre for Genetic Engineering & Biotechnology, New Delhi 110067, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Virology Group, International Centre for Genetic Engineering & Biotechnology, New Delhi 110067, India","institution_ids":["https://openalex.org/I4210142124"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5031518835","display_name":"Sunil Kumar Lal","orcid":"https://orcid.org/0000-0003-3548-4853","observed_orcids":["https://orcid.org/0000-0003-3548-4853"]},"institutions":[{"id":"https://openalex.org/I4210142124","display_name":"International Centre for Genetic Engineering and Biotechnology","ror":"https://ror.org/03j4rrt43","country_code":"IN","type":"facility","lineage":["https://openalex.org/I4210142124","https://openalex.org/I91080651"]}],"countries":["IN"],"is_corresponding":false,"raw_author_name":"Sunil K. Lal","raw_affiliation_strings":["Virology Group, International Centre for Genetic Engineering & Biotechnology, New Delhi 110067, India"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Virology Group, International Centre for Genetic Engineering & Biotechnology, New Delhi 110067, India","institution_ids":["https://openalex.org/I4210142124"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I4210142124"],"apc_list":null,"apc_paid":null,"fwci":1.0875,"has_fulltext":false,"cited_by_count":103,"citation_normalized_percentile":{"value":0.80389084,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":99},"biblio":{"volume":"78","issue":"1","first_page":"320","last_page":"328"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12025","display_name":"Hepatitis Viruses Studies and Epidemiology","score":0.9702000021934509,"subfield":{"id":"https://openalex.org/subfields/2721","display_name":"Hepatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T12025","display_name":"Hepatitis Viruses Studies and Epidemiology","score":0.9702000021934509,"subfield":{"id":"https://openalex.org/subfields/2721","display_name":"Hepatology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11495","display_name":"Animal Virus Infections Studies","score":0.006200000178068876,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10976","display_name":"Viral gastroenteritis research and epidemiology","score":0.0031999999191612005,"subfield":{"id":"https://openalex.org/subfields/2725","display_name":"Infectious Diseases"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/hepatitis-e-virus","display_name":"hepatitis E virus","score":0.9959999918937683},{"id":"https://openalex.org/keywords/orf2-protein","display_name":"ORF2 protein","score":0.921999990940094},{"id":"https://openalex.org/keywords/major-capsid-protein","display_name":"major capsid protein","score":0.8019999861717224},{"id":"https://openalex.org/keywords/protein-rna-interactions","display_name":"RNA-protein interactions","score":0.7910000085830688},{"id":"https://openalex.org/keywords/rna-binding-activity","display_name":"RNA-binding activity","score":0.625},{"id":"https://openalex.org/keywords/orf3-protein","display_name":"ORF3 protein","score":0.7170000076293945},{"id":"https://openalex.org/keywords/viral-encapsidation","display_name":"viral encapsidation","score":0.8420000076293945},{"id":"https://openalex.org/keywords/rna-secondary-structure","display_name":"RNA secondary structure","score":0.7210000157356262}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.8605180978775024},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.6700502038002014},{"id":"https://openalex.org/C202878990","wikidata":"https://www.wikidata.org/wiki/Q830465","display_name":"Capsid","level":3,"score":0.6641832590103149},{"id":"https://openalex.org/C2780777367","wikidata":"https://www.wikidata.org/wiki/Q24757533","display_name":"Hepatitis E virus","level":4,"score":0.5802688598632812},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.5682672262191772},{"id":"https://openalex.org/C141231307","wikidata":"https://www.wikidata.org/wiki/Q7020","display_name":"Genome","level":3,"score":0.5201706886291504},{"id":"https://openalex.org/C142575336","wikidata":"https://www.wikidata.org/wiki/Q412148","display_name":"Polyadenylation","level":4,"score":0.5141425728797913},{"id":"https://openalex.org/C2779174946","wikidata":"https://www.wikidata.org/wiki/Q751911","display_name":"RNA virus","level":4,"score":0.45344194769859314},{"id":"https://openalex.org/C41258723","wikidata":"https://www.wikidata.org/wiki/Q2919111","display_name":"RNA-dependent RNA polymerase","level":4,"score":0.4511777460575104},{"id":"https://openalex.org/C47289529","wikidata":"https://www.wikidata.org/wiki/Q908550","display_name":"Open reading frame","level":4,"score":0.4268229007720947},{"id":"https://openalex.org/C159047783","wikidata":"https://www.wikidata.org/wiki/Q7215","display_name":"Virology","level":1,"score":0.406514972448349},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.34260761737823486},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.3419332802295685},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.29644638299942017},{"id":"https://openalex.org/C135763542","wikidata":"https://www.wikidata.org/wiki/Q106016","display_name":"Genotype","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D001483","descriptor_name":"Base Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006023","descriptor_name":"Glycoproteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D008969","descriptor_name":"Molecular Sequence Data","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009690","descriptor_name":"Nucleic Acid Conformation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010957","descriptor_name":"Plasmids","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D012367","descriptor_name":"RNA, Viral","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012367","descriptor_name":"RNA, Viral","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D016752","descriptor_name":"Hepatitis E virus","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D020121","descriptor_name":"5' Untranslated Regions","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D020121","descriptor_name":"5' Untranslated Regions","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D020798","descriptor_name":"Two-Hybrid System Techniques","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D036022","descriptor_name":"Capsid Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true}],"locations_count":3,"locations":[{"id":"doi:10.1128/jvi.78.1.320-328.2004","doi":"https://doi.org/10.1128/jvi.78.1.320-328.2004","is_oa":false,"landing_page_url":"https://doi.org/10.1128/jvi.78.1.320-328.2004","pdf_url":null,"source":{"id":"https://openalex.org/S96615428","display_name":"Journal of Virology","issn_l":"0022-538X","issn":["0022-538X","1070-6321","1098-5514"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-a","doyens","jufo-2","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Virology","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:303377","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/303377","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Virol","raw_type":"Text"},{"id":"pmid:14671114","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/14671114","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of virology","raw_type":"Journal Article"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:303377","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/303377","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Virol","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2133490887","display_name":"Analysis of protein-protein interactions, signalling pathways and host factors towards understanding the pathogenesis of hepatitis E virus.","funder_award_id":"063171","funder_id":"https://openalex.org/F4320311904","funder_display_name":"Wellcome Trust"}],"funders":[{"id":"https://openalex.org/F4320311904","display_name":"Wellcome Trust","ror":"https://ror.org/029chgv08"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":39,"referenced_works":["https://openalex.org/W437178797","https://openalex.org/W568493077","https://openalex.org/W1526043578","https://openalex.org/W1542686183","https://openalex.org/W1566478858","https://openalex.org/W1585874678","https://openalex.org/W1695296338","https://openalex.org/W1938014745","https://openalex.org/W1962250011","https://openalex.org/W1967776406","https://openalex.org/W1972437755","https://openalex.org/W1986405219","https://openalex.org/W1987780425","https://openalex.org/W1987962702","https://openalex.org/W1996017311","https://openalex.org/W2004249771","https://openalex.org/W2006167038","https://openalex.org/W2012557023","https://openalex.org/W2016461161","https://openalex.org/W2017894098","https://openalex.org/W2032567331","https://openalex.org/W2043422501","https://openalex.org/W2050838285","https://openalex.org/W2052108496","https://openalex.org/W2071827904","https://openalex.org/W2072594662","https://openalex.org/W2075666854","https://openalex.org/W2076969999","https://openalex.org/W2086330724","https://openalex.org/W2100435038","https://openalex.org/W2111246062","https://openalex.org/W2123792518","https://openalex.org/W2130666752","https://openalex.org/W2135793835","https://openalex.org/W2154627118","https://openalex.org/W2167183394","https://openalex.org/W2207950654","https://openalex.org/W2224063625","https://openalex.org/W2480972926"],"related_works":["https://openalex.org/W2014478973","https://openalex.org/W1978273641","https://openalex.org/W2043422501","https://openalex.org/W2531404692","https://openalex.org/W2154627118","https://openalex.org/W2810360101","https://openalex.org/W7134903375","https://openalex.org/W4394590994","https://openalex.org/W2001170827","https://openalex.org/W3026530948"],"abstract_inverted_index":{"Hepatitis":[0],"E":[1],"virus":[2,20,46],"(HEV)":[3],"is":[4,16],"a":[5,17,22,50,141,199,286],"major":[6,41],"human":[7],"pathogen":[8],"in":[9,79,118,140,247,288],"much":[10],"of":[11,27,44,53,72,84,164,170,193,207,234,250,260,271],"the":[12,40,45,57,68,80,85,88,101,116,129,149,161,165,186,194,204,208,215,222,232,248,251,269,272,277],"developing":[13],"world.":[14],"It":[15],"plus-strand":[18],"RNA":[19,69,90,131,153,176,196],"with":[21,134,240],"7.2-kb":[23],"polyadenylated":[24],"genome":[25,210,279],"consisting":[26],"three":[28],"open":[29],"reading":[30],"frames,":[31],"ORF1,":[32],"ORF2,":[33],"and":[34,47,61,74,103,136,242,268],"ORF3.":[35],"Of":[36],"these,":[37],"ORF2":[38,73,135,150,157,216,241,273],"encodes":[39,49],"capsid":[42],"protein":[43,52,151,158,172],"ORF3":[48,137],"small":[51],"unknown":[54],"function.":[55],"Using":[56],"yeast":[58,142],"three-hybrid":[59],"system":[60],"traditional":[62],"biochemical":[63],"techniques,":[64],"we":[65,106],"have":[66],"studied":[67],"binding":[70,154,177,214,274],"activities":[71],"ORF3,":[75],"two":[76,109],"proteins":[77,139],"encoded":[78],"3'":[81,104],"structural":[82],"part":[83],"genome.":[86,167],"Since":[87],"genomic":[89,130],"from":[91],"HEV":[92,166,209,224,278],"has":[93],"been":[94],"postulated":[95],"to":[96,124],"contain":[97],"secondary":[98],"structures":[99],"at":[100,203],"5'":[102,162,205],"ends,":[105],"used":[107],"these":[108],"terminal":[110],"regions,":[111],"besides":[112],"other":[113],"regions":[114],"within":[115,276],"genome,":[117],"this":[119,171,235,261,282],"study.":[120],"Experiments":[121],"were":[122,183,264],"designed":[123],"test":[125],"for":[126,213,238],"interactions":[127],"between":[128],"fusion":[132],"constructs":[133],"hybrid":[138],"cellular":[143],"environment.":[144],"We":[145],"show":[146],"here":[147],"that":[148,174,198,281],"contains":[152],"activity.":[155],"The":[156],"specifically":[159],"bound":[160],"end":[163,206],"Deletion":[168],"analysis":[169],"showed":[173],"its":[175],"activity":[178],"was":[179,211],"lost":[180],"when":[181,254],"deletions":[182],"made":[184],"beyond":[185],"N-terminal":[187],"111":[188],"amino":[189],"acids.":[190],"Finer":[191],"mapping":[192],"interacting":[195],"revealed":[197],"76-nucleotide":[200],"(nt)":[201],"region":[202,220,263,275],"responsible":[212],"protein.":[217],"This":[218],"76-nt":[219,262],"included":[221],"51-nt":[223],"sequence,":[225],"conserved":[226,236],"across":[227],"alphaviruses.":[228],"Our":[229],"results":[230],"support":[231],"requirement":[233],"sequence":[237],"interaction":[239,253,283],"also":[243],"indicate":[244,280],"an":[245,255],"increase":[246],"strength":[249],"RNA-protein":[252],"additional":[256],"44":[257],"bases":[258],"downstream":[259],"included.":[265],"Secondary-structure":[266],"predictions":[267],"location":[270],"may":[284],"play":[285],"role":[287],"viral":[289],"encapsidation.":[290]},"counts_by_year":[{"year":2026,"cited_by_count":2},{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":2},{"year":2023,"cited_by_count":7},{"year":2022,"cited_by_count":4},{"year":2021,"cited_by_count":6},{"year":2020,"cited_by_count":5},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":5},{"year":2017,"cited_by_count":3},{"year":2016,"cited_by_count":12},{"year":2015,"cited_by_count":5},{"year":2014,"cited_by_count":1},{"year":2013,"cited_by_count":6},{"year":2012,"cited_by_count":7}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}