{"id":"https://openalex.org/W2113086466","doi":null,"title":"Functional analysis of UDP-sugar : sterol glucosyltransferases","display_name":"Functional analysis of UDP-sugar : sterol glucosyltransferases","publication_year":2011,"publication_date":"2011-07-01","ids":{"openalex":"https://openalex.org/W2113086466","mag":"2113086466"},"language":"en","primary_location":{"id":"pmh:oai:nrl.northumbria.ac.uk:7259","doi":null,"is_oa":false,"landing_page_url":"https://nrl.northumbria.ac.uk/id/eprint/7259/1/malik.vatsala_phd.pdf","pdf_url":"https://nrl.northumbria.ac.uk/id/eprint/7259/1/malik.vatsala_phd.pdf","source":{"id":"https://openalex.org/S4306401884","display_name":"Northumbria Research Link (Northumbria University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I32394136","host_organization_name":"Northumbria University","host_organization_lineage":["https://openalex.org/I32394136"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},"volume":null,"type":"dissertation","is_traditional":true,"indexed_in":[],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5039409616","display_name":"Vatsala Malik","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I32394136","display_name":"Northumbria University","ror":"https://ror.org/049e6bc10","country_code":"GB","type":"education","lineage":["https://openalex.org/I32394136"]}],"countries":["GB"],"is_corresponding":true,"raw_author_name":"Vatsala Malik","raw_affiliation_strings":["Northumbria University"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Northumbria University","institution_ids":["https://openalex.org/I32394136"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5039409616"],"corresponding_institution_ids":["https://openalex.org/I32394136"],"apc_list":null,"apc_paid":null,"fwci":null,"has_fulltext":false,"cited_by_count":0,"citation_normalized_percentile":null,"cited_by_percentile_year":null,"biblio":{"volume":null,"issue":null,"first_page":null,"last_page":null},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11882","display_name":"Plant biochemistry and biosynthesis","score":0.4749000072479248,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11882","display_name":"Plant biochemistry and biosynthesis","score":0.4749000072479248,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10835","display_name":"Carbohydrate Chemistry and Synthesis","score":0.11900000274181366,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.093299999833107,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/steryl-glycosides","display_name":"sterol glycosides","score":0.859000027179718},{"id":"https://openalex.org/keywords/glycosyltransferases","display_name":"glycosyltransferases","score":0.8429999947547913},{"id":"https://openalex.org/keywords/hansenula-polymorpha","display_name":"Hansenula polymorpha","score":0.9390000104904175},{"id":"https://openalex.org/keywords/kluyveromyces-lactis","display_name":"Kluyveromyces lactis","score":1.0},{"id":"https://openalex.org/keywords/saccharomyces-cerevisiae","display_name":"Saccharomyces cerevisiae","score":0.9779999852180481},{"id":"https://openalex.org/keywords/testosterone","display_name":"testosterone","score":0.5450000166893005},{"id":"https://openalex.org/keywords/enzyme-kinetics","display_name":"enzyme kinetics","score":0.43299999833106995},{"id":"https://openalex.org/keywords/random-mutagenesis","display_name":"random mutagenesis","score":0.8130000233650208},{"id":"https://openalex.org/keywords/homology-modeling","display_name":"homology modeling","score":0.9879999756813049},{"id":"https://openalex.org/keywords/enzyme-cloning-and-expression","display_name":"enzyme cloning and expression","score":0.5389999747276306},{"id":"https://openalex.org/keywords/drug-development","display_name":"drug development","score":0.5360000133514404}],"concepts":[{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6431673169136047},{"id":"https://openalex.org/C2910007386","wikidata":"https://www.wikidata.org/wiki/Q5572361","display_name":"Glucosyltransferases","level":3,"score":0.6021860837936401},{"id":"https://openalex.org/C2777576037","wikidata":"https://www.wikidata.org/wiki/Q719725","display_name":"Saccharomyces cerevisiae","level":3,"score":0.6019414067268372},{"id":"https://openalex.org/C2778041695","wikidata":"https://www.wikidata.org/wiki/Q6421540","display_name":"Kluyveromyces lactis","level":4,"score":0.581736147403717},{"id":"https://openalex.org/C161019410","wikidata":"https://www.wikidata.org/wiki/Q67201373","display_name":"Glycosyltransferase","level":3,"score":0.519858181476593},{"id":"https://openalex.org/C2778718757","wikidata":"https://www.wikidata.org/wiki/Q143623","display_name":"Sterol","level":3,"score":0.4942600727081299},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.4346548616886139},{"id":"https://openalex.org/C553450214","wikidata":"https://www.wikidata.org/wiki/Q851162","display_name":"Biosynthesis","level":3,"score":0.42467620968818665},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.35402536392211914},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.3032459616661072},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.2519213557243347},{"id":"https://openalex.org/C2778163477","wikidata":"https://www.wikidata.org/wiki/Q43656","display_name":"Cholesterol","level":2,"score":0.0}],"mesh":[],"locations_count":4,"locations":[{"id":"pmh:oai:nrl.northumbria.ac.uk:7259","doi":null,"is_oa":false,"landing_page_url":"https://nrl.northumbria.ac.uk/id/eprint/7259/1/malik.vatsala_phd.pdf","pdf_url":"https://nrl.northumbria.ac.uk/id/eprint/7259/1/malik.vatsala_phd.pdf","source":{"id":"https://openalex.org/S4306401884","display_name":"Northumbria Research Link (Northumbria University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I32394136","host_organization_name":"Northumbria University","host_organization_lineage":["https://openalex.org/I32394136"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"","raw_type":"Thesis"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.465.1717","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.465.1717","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://nrl.northumbria.ac.uk/7259/1/malik_phd.pdf","raw_type":"text"},{"id":"pmh:hdl:10068/993178","doi":null,"is_oa":false,"landing_page_url":"http://hdl.handle.net/10068/993178","pdf_url":null,"source":{"id":"https://openalex.org/S4377196900","display_name":"OpenGrey (Institut de l'Information Scientifique et Technique)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1295345811","host_organization_name":"Institut de l'Information Scientifique et Technique","host_organization_lineage":["https://openalex.org/I1295345811"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"U - Thesis"},{"id":"mag:2113086466","doi":null,"is_oa":false,"landing_page_url":"http://nrl.northumbria.ac.uk/7259/1/malik.vatsala_phd.pdf","pdf_url":null,"source":null,"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":null,"raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":163,"referenced_works":["https://openalex.org/W27661714","https://openalex.org/W37279920","https://openalex.org/W339055328","https://openalex.org/W1480803047","https://openalex.org/W1485020764","https://openalex.org/W1566767862","https://openalex.org/W1761905744","https://openalex.org/W1907206755","https://openalex.org/W1912870130","https://openalex.org/W1964092459","https://openalex.org/W1964408716","https://openalex.org/W1965500550","https://openalex.org/W1966163617","https://openalex.org/W1966678026","https://openalex.org/W1969021085","https://openalex.org/W1970809419","https://openalex.org/W1971596940","https://openalex.org/W1974410548","https://openalex.org/W1974772724","https://openalex.org/W1976483127","https://openalex.org/W1976609472","https://openalex.org/W1976888209","https://openalex.org/W1977950017","https://openalex.org/W1981508052","https://openalex.org/W1985453415","https://openalex.org/W1987362712","https://openalex.org/W1988239715","https://openalex.org/W1988733774","https://openalex.org/W1993019691","https://openalex.org/W1995246141","https://openalex.org/W1995548272","https://openalex.org/W1995727180","https://openalex.org/W1995856279","https://openalex.org/W1997439284","https://openalex.org/W1999109941","https://openalex.org/W1999249189","https://openalex.org/W2000462994","https://openalex.org/W2000777444","https://openalex.org/W2001423392","https://openalex.org/W2002254535","https://openalex.org/W2004917508","https://openalex.org/W2010620034","https://openalex.org/W2011380937","https://openalex.org/W2011830019","https://openalex.org/W2013130117","https://openalex.org/W2015334615","https://openalex.org/W2017699758","https://openalex.org/W2018121351","https://openalex.org/W2018454041","https://openalex.org/W2020567118","https://openalex.org/W2021436828","https://openalex.org/W2022988743","https://openalex.org/W2023627112","https://openalex.org/W2025178332","https://openalex.org/W2027680546","https://openalex.org/W2030038843","https://openalex.org/W2031313159","https://openalex.org/W2031712092","https://openalex.org/W2035103700","https://openalex.org/W2036344160","https://openalex.org/W2038104565","https://openalex.org/W2039048594","https://openalex.org/W2041112826","https://openalex.org/W2044509450","https://openalex.org/W2044777100","https://openalex.org/W2045377815","https://openalex.org/W2046867131","https://openalex.org/W2049905838","https://openalex.org/W2050917919","https://openalex.org/W2051255976","https://openalex.org/W2052561661","https://openalex.org/W2052819253","https://openalex.org/W2053216795","https://openalex.org/W2053397996","https://openalex.org/W2053470037","https://openalex.org/W2054155771","https://openalex.org/W2054922107","https://openalex.org/W2056961674","https://openalex.org/W2057960863","https://openalex.org/W2061325528","https://openalex.org/W2061348097","https://openalex.org/W2061659012","https://openalex.org/W2063271557","https://openalex.org/W2063950340","https://openalex.org/W2063951650","https://openalex.org/W2064322162","https://openalex.org/W2065245548","https://openalex.org/W2067074380","https://openalex.org/W2072651233","https://openalex.org/W2072672406","https://openalex.org/W2074570966","https://openalex.org/W2074695441","https://openalex.org/W2075814912","https://openalex.org/W2075821413","https://openalex.org/W2077417665","https://openalex.org/W2077603133","https://openalex.org/W2078532751","https://openalex.org/W2078917933","https://openalex.org/W2079450238","https://openalex.org/W2079505033","https://openalex.org/W2079870230","https://openalex.org/W2082287966","https://openalex.org/W2083190097","https://openalex.org/W2086194820","https://openalex.org/W2086427371","https://openalex.org/W2089047063","https://openalex.org/W2089065678","https://openalex.org/W2091296296","https://openalex.org/W2092299098","https://openalex.org/W2092471412","https://openalex.org/W2094470156","https://openalex.org/W2094677232","https://openalex.org/W2095106731","https://openalex.org/W2095529535","https://openalex.org/W2095997872","https://openalex.org/W2096275360","https://openalex.org/W2097848666","https://openalex.org/W2099927731","https://openalex.org/W2100145719","https://openalex.org/W2100445216","https://openalex.org/W2100837269","https://openalex.org/W2103963569","https://openalex.org/W2108929776","https://openalex.org/W2110921177","https://openalex.org/W2112921913","https://openalex.org/W2120288510","https://openalex.org/W2122322467","https://openalex.org/W2122589838","https://openalex.org/W2124230467","https://openalex.org/W2125647971","https://openalex.org/W2126483682","https://openalex.org/W2129092523","https://openalex.org/W2129712803","https://openalex.org/W2130581521","https://openalex.org/W2131520472","https://openalex.org/W2131617047","https://openalex.org/W2134038950","https://openalex.org/W2135949063","https://openalex.org/W2137441463","https://openalex.org/W2141589632","https://openalex.org/W2141885858","https://openalex.org/W2142464210","https://openalex.org/W2144634347","https://openalex.org/W2145832204","https://openalex.org/W2146664263","https://openalex.org/W2150371887","https://openalex.org/W2151031764","https://openalex.org/W2151891111","https://openalex.org/W2151974289","https://openalex.org/W2154170463","https://openalex.org/W2155305442","https://openalex.org/W2162331245","https://openalex.org/W2162544563","https://openalex.org/W2165750119","https://openalex.org/W2167958276","https://openalex.org/W2168056658","https://openalex.org/W2171087302","https://openalex.org/W2172024836","https://openalex.org/W2331954809","https://openalex.org/W2398736662","https://openalex.org/W2415171719","https://openalex.org/W2952301445","https://openalex.org/W3150239599"],"related_works":["https://openalex.org/W7099709610","https://openalex.org/W2018121351","https://openalex.org/W4220667857","https://openalex.org/W2899344442","https://openalex.org/W1499938999","https://openalex.org/W2049651968","https://openalex.org/W473152972","https://openalex.org/W4319985826","https://openalex.org/W7155074493","https://openalex.org/W4323049123"],"abstract_inverted_index":{"Glycosyltransferases":[0],"(GTs)":[1],"are":[2],"essential":[3],"for":[4,80,170,191],"the":[5,23,33,50,53,57,65,101,126,140,154,180,202,209],"biosynthesis":[6],"and":[7,64,67,89,96,111,136,156,190],"diversification":[8],"of":[9,25,52,69,87,104,134,139,173,179,187,204,211],"many":[10],"therapeutically":[11,26],"important":[12,27],"natural":[13],"products.":[14],"Of":[15],"these,":[16],"UDP-sugar:":[17],"sterol":[18,88],"glucosyltransferases":[19],"(UGTs)":[20],"(2.4.1.173)":[21],"catalyse":[22],"synthesis":[24,210],"steryl":[28],"glycosides":[29],"(SGs).":[30],"Guided":[31],"by":[32,122],"sequence":[34],"similarity":[35],"with":[36,147,183],"a":[37,85,168,184,205],"previously":[38,127],"characterised":[39,128],"N-terminally":[40,74],"truncated":[41],"UGT":[42,143],"from":[43,61,108,176],"Saccharomyces":[44],"cerevisiae":[45,130,142],"(UGT51),":[46],"this":[47,164],"study":[48],"reports":[49],"cloning":[51],"gene":[54],"fragment":[55],"encoding":[56],"C-terminal":[58],"catalytic":[59,188],"domains":[60,189],"related":[62],"yeasts":[63],"expression":[66],"characterisation":[68,103],"their":[70,193],"encoded":[71],"products":[72],"produced.":[73],"histidine":[75],"tagged":[76],"proteins":[77],"were":[78],"purified":[79],"in":[81,195,208],"vitro":[82],"assays":[83],"against":[84],"panel":[86],"steroidal":[90],"acceptors.":[91],"Liquid":[92],"chromatography-mass":[93],"spectrometry":[94],"(LC-MS)":[95],"kinetic":[97],"analysis":[98],"led":[99],"to":[100,119],"successful":[102],"two":[105],"novel":[106,174],"UGTs":[107,135,175],"Pichia":[109],"angusta":[110],"Kluyveromyces":[112],"lactis.":[113],"In":[114],"addition,":[115],"testosterone":[116],"was":[117],"shown":[118],"be":[120],"utilized":[121],"all":[123],"UGTs,":[124,163],"including":[125],"S.":[129,141],"UGT51.":[131],"Random":[132],"mutagenesis":[133],"homology":[137],"modelling":[138],"revealed":[144],"structural":[145,155],"similarities":[146,158],"family":[148,161],"1":[149,162],"bacterial":[150],"glycopeptide":[151],"GTs.":[152],"Given":[153],"mechanistic":[157],"among":[159],"GT":[160,181],"approach":[165],"may":[166,199],"provide":[167],"template":[169],"genetic":[171],"manipulation":[172],"other":[177],"members":[178],"superfamily":[182],"better":[185],"understanding":[186],"broadening":[192],"scope":[194],"drug":[196],"development.":[197],"It":[198],"also":[200],"aid":[201],"development":[203],"generic":[206],"process":[207],"SGs.":[212]},"counts_by_year":[],"updated_date":"2026-09-29T14:09:16.559479","created_date":"2016-06-24T00:00:00"}
