{"id":"https://openalex.org/W2109960401","doi":"https://doi.org/10.3109/10520295409115453","title":"Squash Method for Meiosis in Ovules","display_name":"Squash Method for Meiosis in Ovules","publication_year":1954,"publication_date":"1954-01-01","ids":{"openalex":"https://openalex.org/W2109960401","doi":"https://doi.org/10.3109/10520295409115453","mag":"2109960401","pmid":"https://pubmed.ncbi.nlm.nih.gov/13156794"},"language":"en","primary_location":{"id":"doi:10.3109/10520295409115453","doi":"https://doi.org/10.3109/10520295409115453","is_oa":false,"landing_page_url":"https://doi.org/10.3109/10520295409115453","pdf_url":null,"source":{"id":"https://openalex.org/S4210226617","display_name":"Stain Technology","issn_l":"0038-9153","issn":["0038-9153"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Stain Technology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Ashraful Haque","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I40120149","display_name":"University of Oxford","ror":"https://ror.org/052gg0110","country_code":"GB","type":"education","lineage":["https://openalex.org/I40120149"]}],"countries":["GB"],"is_corresponding":true,"raw_author_name":"Ashraful Haque","raw_affiliation_strings":["Department of Botany, University of Oxford, Oxford, England"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Botany, University of Oxford, Oxford, England","institution_ids":["https://openalex.org/I40120149"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I40120149"],"apc_list":null,"apc_paid":null,"fwci":0.3585,"has_fulltext":false,"cited_by_count":6,"citation_normalized_percentile":{"value":0.570876,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"29","issue":"3","first_page":"109","last_page":"112"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11927","display_name":"Plant Reproductive Biology","score":0.5497999787330627,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11927","display_name":"Plant Reproductive Biology","score":0.5497999787330627,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10434","display_name":"Chromosomal and Genetic Variations","score":0.3792000114917755,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12568","display_name":"Plant Taxonomy and Phylogenetics","score":0.012799999676644802,"subfield":{"id":"https://openalex.org/subfields/1105","display_name":"Ecology, Evolution, Behavior and Systematics"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/meiosis","display_name":"meiosis","score":0.8529999852180481},{"id":"https://openalex.org/keywords/ovule","display_name":"ovule","score":0.9559999704360962},{"id":"https://openalex.org/keywords/squash-technique","display_name":"squash technique","score":0.7760000228881836},{"id":"https://openalex.org/keywords/feulgen-staining","display_name":"Feulgen staining","score":0.9470000267028809},{"id":"https://openalex.org/keywords/megaspore-mother-cell","display_name":"megaspore mother cell","score":0.9539999961853027},{"id":"https://openalex.org/keywords/integument","display_name":"integument","score":0.6069999933242798},{"id":"https://openalex.org/keywords/nucellus","display_name":"nucellus","score":0.9879999756813049},{"id":"https://openalex.org/keywords/plant-cell-biology","display_name":"plant cell biology","score":0.43299999833106995}],"concepts":[{"id":"https://openalex.org/C2776673659","wikidata":"https://www.wikidata.org/wiki/Q47512","display_name":"Acetic acid","level":2,"score":0.7538474798202515},{"id":"https://openalex.org/C74864618","wikidata":"https://www.wikidata.org/wiki/Q2332446","display_name":"Staining","level":2,"score":0.6867051720619202},{"id":"https://openalex.org/C2779429693","wikidata":"https://www.wikidata.org/wiki/Q274959","display_name":"Distilled water","level":2,"score":0.6453320980072021},{"id":"https://openalex.org/C2781294515","wikidata":"https://www.wikidata.org/wiki/Q2733470","display_name":"Stain","level":3,"score":0.5345240235328674},{"id":"https://openalex.org/C62649737","wikidata":"https://www.wikidata.org/wiki/Q902526","display_name":"Feulgen stain","level":3,"score":0.5137337446212769},{"id":"https://openalex.org/C92324200","wikidata":"https://www.wikidata.org/wiki/Q380138","display_name":"Ovule","level":3,"score":0.5022661685943604},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.43250012397766113},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.34111467003822327},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.30165427923202515},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.2704727053642273},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.21546003222465515},{"id":"https://openalex.org/C2780618852","wikidata":"https://www.wikidata.org/wiki/Q79932","display_name":"Pollen","level":2,"score":0.10054019093513489},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D002455","descriptor_name":"Cell Division","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D004396","descriptor_name":"Coloring Agents","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D008540","descriptor_name":"Meiosis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D011058","descriptor_name":"Pollen","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D013194","descriptor_name":"Staining and Labeling","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D056771","descriptor_name":"Ovule","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":2,"locations":[{"id":"doi:10.3109/10520295409115453","doi":"https://doi.org/10.3109/10520295409115453","is_oa":false,"landing_page_url":"https://doi.org/10.3109/10520295409115453","pdf_url":null,"source":{"id":"https://openalex.org/S4210226617","display_name":"Stain Technology","issn_l":"0038-9153","issn":["0038-9153"],"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Stain Technology","raw_type":"journal-article"},{"id":"pmid:13156794","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/13156794","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Stain technology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":0,"referenced_works":[],"related_works":["https://openalex.org/W2337855869","https://openalex.org/W2031754427","https://openalex.org/W1930582611","https://openalex.org/W2022626208","https://openalex.org/W7084252633","https://openalex.org/W1964101809","https://openalex.org/W2057051101","https://openalex.org/W2021463719","https://openalex.org/W1970615035","https://openalex.org/W2474311393"],"abstract_inverted_index":{"The":[0,85,219,234,252],"ordinary":[1],"Feulgen":[2,60,182],"or":[3,177,183],"acetic-lacmoid":[4,121,184],"squash":[5],"tech-nic":[6],"following":[7],"fixation":[8,34],"in":[9,31,42,68,77,170,259,264],"freshly":[10],"made":[11,255],"Carnoy's":[12],"fluid":[13],"(alcohol,":[14],"6:":[15],"chloroform,":[16],"3:":[17],"glacial":[18],"acetic":[19,104,175],"acid,":[20],"1),":[21],"provides":[22],"an":[23],"easy":[24],"and":[25,49,115,188,204,206,249,262],"reliable":[26],"method":[27],"of":[28,129,137,173,202,231,237],"studying":[29],"meiosis":[30],"ovules.":[32],"After":[33,95,150],"for":[35,46,82],"1":[36,69,127,130],"day,":[37],"the":[38,59,62,91,98,110,144,157,181,192,200,209,228,232,246],"material":[39,63,92,158],"was":[40,64,87,100,125,141,159,221,239],"hardened":[41],"95%":[43],"ethyl":[44,260],"alcohol":[45,261],"1\u20132":[47],"days":[48],"taken":[50],"to":[51,93,119,123,133,147,161,217],"water":[52,99],"by":[53,58,75,89,102,108,195,242,257],"gradual":[54],"hydration.":[55],"For":[56,106],"staining":[57,76,86,107,185],"method,":[61],"hydrolyzed":[65],"8\u201310":[66],"minutes":[67,97,153],"N":[70,131],"HO":[71],"at":[72,154],"58\u201360\u00b0C.,":[73],"followed":[74],"decolorized":[78],"leuco":[79],"basic":[80],"fuchsin":[81],"2":[83],"hours.":[84],"intensified":[88],"transferring":[90],"water.":[94],"15\u201320":[96],"replaced":[101],"45%":[103,174],"acid.":[105],"acetic-lacmoid,":[109,178],"ovules":[111,166],"after":[112],"fixation,":[113],"hardening":[114],"hydration":[116],"were":[117,167,254],"transferred":[118,160],"standard":[120],"stain":[122,145],"which":[124],"added":[126],"drop":[128,172],"HCl":[132],"every":[134],"10":[135],"drops":[136],"stain.":[138],"Gentle":[139,187],"heat":[140],"applied":[142],"till":[143],"started":[146],"give":[148],"fumes.":[149],"allowing":[151],"20":[152],"room":[155],"temperature":[156],"fresh":[162],"acetic-lacmoid.":[163],"Some":[164],"6\u201312":[165],"mounted":[168],"either":[169],"a":[171,196],"acid":[176],"depending":[179],"upon":[180],"respectively.":[186],"repeated":[189],"tapping":[190],"over":[191,245],"cover":[193,247],"glass":[194,248],"blunt":[197],"needle":[198],"loosened":[199],"cells":[201,212],"integument":[203],"nucellus":[205],"finally":[207],"left":[208],"megaspore":[210],"mother":[211],"undergoing":[213],"meiosis,":[214],"fully":[215],"exposed":[216],"view.":[218],"process":[220],"carried":[222],"out":[223],"under":[224],"constant":[225],"observation":[226],"using":[227],"low":[229],"power":[230],"microscope.":[233],"desired":[235],"amount":[236],"flattening":[238],"brought":[240],"about":[241],"light":[243],"pressure":[244],"gentle":[250],"heating.":[251],"preparations":[253],"permanent":[256],"dehydrating":[258],"mounting":[263],"Euparal.":[265]},"counts_by_year":[{"year":2023,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}