{"id":"https://openalex.org/W2109539733","doi":"https://doi.org/10.1107/s0907444900008167","title":"Crystallization and structure determination of the catalytic trimer ofMethanococcus jannaschiiaspartate transcarbamoylase","display_name":"Crystallization and structure determination of the catalytic trimer ofMethanococcus jannaschiiaspartate transcarbamoylase","publication_year":2000,"publication_date":"2000-08-01","ids":{"openalex":"https://openalex.org/W2109539733","doi":"https://doi.org/10.1107/s0907444900008167","mag":"2109539733","pmid":"https://pubmed.ncbi.nlm.nih.gov/10944354"},"language":"en","primary_location":{"id":"doi:10.1107/s0907444900008167","doi":"https://doi.org/10.1107/s0907444900008167","is_oa":false,"landing_page_url":"https://doi.org/10.1107/s0907444900008167","pdf_url":null,"source":{"id":"https://openalex.org/S114502960","display_name":"Acta Crystallographica Section D Biological Crystallography","issn_l":"0907-4449","issn":["0907-4449","1399-0047"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Acta Crystallographica Section D Biological Crystallography","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://engagedscholarship.csuohio.edu/sciphysics_facpub/185","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5015599725","display_name":"Jacqueline Vitali","orcid":"https://orcid.org/0000-0003-3029-8496","observed_orcids":["https://orcid.org/0000-0003-3029-8496"]},"institutions":[{"id":"https://openalex.org/I103531236","display_name":"Boston College","ror":"https://ror.org/02n2fzt79","country_code":"US","type":"education","lineage":["https://openalex.org/I103531236"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Jacqueline Vitali","raw_affiliation_strings":["Department of Chemistry, Merkert Chemistry Center, Boston College, Chestnut Hill, MA 02467, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Merkert Chemistry Center, Boston College, Chestnut Hill, MA 02467, USA","institution_ids":["https://openalex.org/I103531236"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5051118925","display_name":"Tatyana Vorobyova","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I103531236","display_name":"Boston College","ror":"https://ror.org/02n2fzt79","country_code":"US","type":"education","lineage":["https://openalex.org/I103531236"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Tatyana Vorobyova","raw_affiliation_strings":["Department of Chemistry, Merkert Chemistry Center, Boston College, Chestnut Hill, MA\u00a002467, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Merkert Chemistry Center, Boston College, Chestnut Hill, MA\u00a002467, USA","institution_ids":["https://openalex.org/I103531236"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5005994721","display_name":"Gordon D. Webster","orcid":"https://orcid.org/0009-0009-2862-0467","observed_orcids":["https://orcid.org/0009-0009-2862-0467"]},"institutions":[{"id":"https://openalex.org/I1316535847","display_name":"Beth Israel Deaconess Medical Center","ror":"https://ror.org/04drvxt59","country_code":"US","type":"healthcare","lineage":["https://openalex.org/I1316535847"]},{"id":"https://openalex.org/I136199984","display_name":"Harvard University","ror":"https://ror.org/03vek6s52","country_code":"US","type":"education","lineage":["https://openalex.org/I136199984"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Gordon Webster","raw_affiliation_strings":["Division of Experimental Medicine, Beth Israel Deaconess Medical Center, Harvard Institutes of Medicine, 4\u00a0Blackfan Circle, Boston, MA 02115, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Division of Experimental Medicine, Beth Israel Deaconess Medical Center, Harvard Institutes of Medicine, 4\u00a0Blackfan Circle, Boston, MA 02115, USA","institution_ids":["https://openalex.org/I1316535847","https://openalex.org/I136199984"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5105414035","display_name":"Evan R. Kantrowitz","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I103531236","display_name":"Boston College","ror":"https://ror.org/02n2fzt79","country_code":"US","type":"education","lineage":["https://openalex.org/I103531236"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Evan R. Kantrowitz","raw_affiliation_strings":["Department of Chemistry, Merkert Chemistry Center, Boston College, Chestnut Hill, MA\u00a002467, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, Merkert Chemistry Center, Boston College, Chestnut Hill, MA\u00a002467, USA","institution_ids":["https://openalex.org/I103531236"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":{"value":4740,"currency":"USD","value_usd":4740},"apc_paid":null,"fwci":0.2819,"has_fulltext":true,"cited_by_count":5,"citation_normalized_percentile":{"value":0.60372132,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":90,"max":94},"biblio":{"volume":"56","issue":"8","first_page":"1061","last_page":"1063"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.8179000020027161,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},"topics":[{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.8179000020027161,"subfield":{"id":"https://openalex.org/subfields/2505","display_name":"Materials Chemistry"},"field":{"id":"https://openalex.org/fields/25","display_name":"Materials Science"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T12827","display_name":"Biochemical and Molecular Research","score":0.17659999430179596,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10044","display_name":"Protein Structure and Dynamics","score":0.0032999999821186066,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/aspartate-transcarbamoylase","display_name":"aspartate transcarbamylase","score":0.9980000257492065},{"id":"https://openalex.org/keywords/methanocaldococcus-jannaschii","display_name":"Methanococcus jannaschii","score":0.972000002861023},{"id":"https://openalex.org/keywords/enzyme-crystallization","display_name":"enzyme crystallization","score":0.6480000019073486},{"id":"https://openalex.org/keywords/thermal-stability","display_name":"thermal stability","score":0.628000020980835},{"id":"https://openalex.org/keywords/pyrimidine-biosynthesis","display_name":"pyrimidine synthesis","score":0.8899999856948853},{"id":"https://openalex.org/keywords/archaeal-enzyme","display_name":"archaeal enzyme","score":0.6370000243186951},{"id":"https://openalex.org/keywords/crystal-structure-determination","display_name":"crystal structure determination","score":0.5830000042915344},{"id":"https://openalex.org/keywords/patterson-function","display_name":"Patterson function","score":0.6980000138282776},{"id":"https://openalex.org/keywords/molecular-replacement","display_name":"molecular replacement","score":0.6129999756813049}],"concepts":[{"id":"https://openalex.org/C197248121","wikidata":"https://www.wikidata.org/wiki/Q24724427","display_name":"Aspartate carbamoyltransferase","level":4,"score":0.9749597907066345},{"id":"https://openalex.org/C2776878370","wikidata":"https://www.wikidata.org/wiki/Q2711364","display_name":"Methanococcus","level":4,"score":0.933502197265625},{"id":"https://openalex.org/C2776403692","wikidata":"https://www.wikidata.org/wiki/Q898416","display_name":"Trimer","level":3,"score":0.795440673828125},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5178087949752808},{"id":"https://openalex.org/C2781216036","wikidata":"https://www.wikidata.org/wiki/Q902494","display_name":"Thermostability","level":3,"score":0.5162351131439209},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.489950567483902},{"id":"https://openalex.org/C8010536","wikidata":"https://www.wikidata.org/wiki/Q160398","display_name":"Crystallography","level":1,"score":0.4165567457675934},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.22825190424919128},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.1955186426639557},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.11553451418876648},{"id":"https://openalex.org/C166342909","wikidata":"https://www.wikidata.org/wiki/Q845326","display_name":"Allosteric regulation","level":3,"score":0.10881400108337402},{"id":"https://openalex.org/C550995028","wikidata":"https://www.wikidata.org/wiki/Q10872","display_name":"Archaea","level":3,"score":0.0836101770401001},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C2779546866","wikidata":"https://www.wikidata.org/wiki/Q531306","display_name":"Dimer","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D001221","descriptor_name":"Aspartate Carbamoyltransferase","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D003460","descriptor_name":"Crystallization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008958","descriptor_name":"Models, Molecular","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017017","descriptor_name":"Methanococcus","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D018360","descriptor_name":"Crystallography, X-Ray","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020836","descriptor_name":"Protein Structure, Quaternary","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1107/s0907444900008167","doi":"https://doi.org/10.1107/s0907444900008167","is_oa":false,"landing_page_url":"https://doi.org/10.1107/s0907444900008167","pdf_url":null,"source":{"id":"https://openalex.org/S114502960","display_name":"Acta Crystallographica Section D Biological Crystallography","issn_l":"0907-4449","issn":["0907-4449","1399-0047"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Acta Crystallographica Section D Biological Crystallography","raw_type":"journal-article"},{"id":"pmid:10944354","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10944354","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Acta crystallographica. Section D, Biological crystallography","raw_type":"Journal Article"},{"id":"pmh:oai:engagedscholarship.csuohio.edu:sciphysics_facpub-1184","doi":null,"is_oa":true,"landing_page_url":"https://engagedscholarship.csuohio.edu/sciphysics_facpub/185","pdf_url":"https://engagedscholarship.csuohio.edu/sciphysics_facpub/185","source":{"id":"https://openalex.org/S4306400889","display_name":"EngagedScholarship @ Cleveland State University (Cleveland State University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I102607778","host_organization_name":"Cleveland State University","host_organization_lineage":["https://openalex.org/I102607778"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Physics Faculty Publications","raw_type":"text"}],"best_oa_location":{"id":"pmh:oai:engagedscholarship.csuohio.edu:sciphysics_facpub-1184","doi":null,"is_oa":true,"landing_page_url":"https://engagedscholarship.csuohio.edu/sciphysics_facpub/185","pdf_url":"https://engagedscholarship.csuohio.edu/sciphysics_facpub/185","source":{"id":"https://openalex.org/S4306400889","display_name":"EngagedScholarship @ Cleveland State University (Cleveland State University)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I102607778","host_organization_name":"Cleveland State University","host_organization_lineage":["https://openalex.org/I102607778"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"Physics Faculty Publications","raw_type":"text"},"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G2333914548","display_name":null,"funder_award_id":"GM26237","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G724647883","display_name":"The Molecular Basis of Cellular Control Mechanisms","funder_award_id":"R01GM026237","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"}],"has_content":{"pdf":true,"grobid_xml":true},"content_urls":{"pdf":"https://content.openalex.org/works/W2109539733.pdf","grobid_xml":"https://content.openalex.org/works/W2109539733.grobid-xml"},"referenced_works_count":18,"referenced_works":["https://openalex.org/W1506226772","https://openalex.org/W1539796472","https://openalex.org/W1565129749","https://openalex.org/W1603300564","https://openalex.org/W1606360219","https://openalex.org/W1652691445","https://openalex.org/W1978181627","https://openalex.org/W1996998147","https://openalex.org/W2012199270","https://openalex.org/W2025032443","https://openalex.org/W2047595408","https://openalex.org/W2054016635","https://openalex.org/W2081076206","https://openalex.org/W2081479690","https://openalex.org/W2084763593","https://openalex.org/W2116275717","https://openalex.org/W2144634347","https://openalex.org/W2335150801"],"related_works":["https://openalex.org/W2086203221","https://openalex.org/W1966271394","https://openalex.org/W2069712200","https://openalex.org/W7153794340","https://openalex.org/W2054016635","https://openalex.org/W2010019361","https://openalex.org/W1974692406","https://openalex.org/W2023915957","https://openalex.org/W2086032634","https://openalex.org/W2039142366"],"abstract_inverted_index":{"Aspartate":[0],"transcarbamoylase":[1],"(ATCase)":[2],"catalyzes":[3],"the":[4,8,12,28,48,55,58,64,89,111,121,125,136,141],"first":[5,62],"step":[6],"in":[7,42,69,88,110,124],"pyrimidine":[9],"biosynthetic":[10],"pathway,":[11],"reaction":[13],"between":[14],"carbamoyl":[15],"phosphate":[16],"and":[17,22,85,99,133],"L-aspartate":[18],"to":[19,44,116,130],"form":[20],"N-carbamoyl-L-aspartate":[21],"phosphate.":[23],"The":[24],"structural":[25,49],"analysis":[26],"of":[27,57,108,135],"ATCase":[29],"catalytic":[30,65],"trimer":[31,66],"from":[32],"Methanococcus":[33],"jannaschii,":[34],"a":[35,61,76],"unicellular":[36],"thermophilic":[37],"archaeabacterium,":[38],"has":[39],"been":[40],"undertaken":[41],"order":[43],"gain":[45],"insight":[46],"into":[47],"features":[50],"that":[51],"are":[52,114,128],"responsible":[53],"for":[54],"thermostability":[56],"enzyme.":[59],"As":[60],"step,":[63],"was":[67,94],"crystallized":[68],"space":[70],"group":[71],"R32,":[72],"with":[73],"unit-cell":[74],"parameters":[75],"=":[77,79,82],"b":[78],"265.3,":[80],"c":[81],"195.5":[83],"A":[84],"two":[86,122],"trimers":[87,123],"asymmetric":[90,112,126],"unit.":[91],"Its":[92],"structure":[93,142],"determined":[95],"using":[96],"molecular":[97],"replacement":[98],"Patterson":[100,137],"methods.":[101],"In":[102,118],"general,":[103],"structures":[104],"containing":[105],"multiple":[106],"copies":[107],"molecules":[109],"unit":[113,127],"difficult":[115],"determine.":[117],"this":[119],"case,":[120],"parallel":[129],"each":[131],"other":[132],"use":[134],"function":[138],"greatly":[139],"simplified":[140],"solution.":[143]},"counts_by_year":[{"year":2025,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}