{"id":"https://openalex.org/W2109474718","doi":"https://doi.org/10.1093/biolreprod/66.4.1169","title":"Heat Shock-Induced Apoptosis in Preimplantation Bovine Embryos Is a Developmentally Regulated Phenomenon1","display_name":"Heat Shock-Induced Apoptosis in Preimplantation Bovine Embryos Is a Developmentally Regulated Phenomenon1","publication_year":2002,"publication_date":"2002-04-01","ids":{"openalex":"https://openalex.org/W2109474718","doi":"https://doi.org/10.1093/biolreprod/66.4.1169","mag":"2109474718","pmid":"https://pubmed.ncbi.nlm.nih.gov/11906938"},"language":"en","primary_location":{"id":"doi:10.1093/biolreprod/66.4.1169","doi":"https://doi.org/10.1093/biolreprod/66.4.1169","is_oa":false,"landing_page_url":"https://doi.org/10.1093/biolreprod/66.4.1169","pdf_url":null,"source":{"id":"https://openalex.org/S130598054","display_name":"Biology of Reproduction","issn_l":"0006-3363","issn":["0006-3363","1529-7268"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biology of Reproduction","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5016857071","display_name":"Fabiola Freitas de Paula-Lopes","orcid":"https://orcid.org/0000-0001-5009-3450","observed_orcids":["https://orcid.org/0000-0001-5009-3450"]},"institutions":[{"id":"https://openalex.org/I33213144","display_name":"University of Florida","ror":"https://ror.org/02y3ad647","country_code":"US","type":"education","lineage":["https://openalex.org/I33213144"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"F.F. Paula-Lopes","raw_affiliation_strings":["Department of Animal Sciences, University of Florida, Gainesville, FL 32611-0910, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Animal Sciences, University of Florida, Gainesville, FL 32611-0910, USA","institution_ids":["https://openalex.org/I33213144"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5028606515","display_name":"Peter James Hansen","orcid":"https://orcid.org/0000-0003-3061-9333","observed_orcids":["https://orcid.org/0000-0003-3061-9333"]},"institutions":[{"id":"https://openalex.org/I33213144","display_name":"University of Florida","ror":"https://ror.org/02y3ad647","country_code":"US","type":"education","lineage":["https://openalex.org/I33213144"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"P.J. Hansen","raw_affiliation_strings":["Department of Animal Sciences, University of Florida, Gainesville, Florida 32611-0910"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Animal Sciences, University of Florida, Gainesville, Florida 32611-0910","institution_ids":["https://openalex.org/I33213144"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5028606515"],"corresponding_institution_ids":["https://openalex.org/I33213144"],"apc_list":{"value":4605,"currency":"USD","value_usd":4605},"apc_paid":null,"fwci":10.9747,"has_fulltext":false,"cited_by_count":210,"citation_normalized_percentile":{"value":0.98768214,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":96,"max":100},"biblio":{"volume":"66","issue":"4","first_page":"1169","last_page":"1177"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10364","display_name":"Reproductive Biology and Fertility","score":0.5062999725341797,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10364","display_name":"Reproductive Biology and Fertility","score":0.5062999725341797,"subfield":{"id":"https://openalex.org/subfields/2739","display_name":"Public Health, Environmental and Occupational Health"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10584","display_name":"Reproductive Physiology in Livestock","score":0.25459998846054077,"subfield":{"id":"https://openalex.org/subfields/1102","display_name":"Agronomy and Crop Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12365","display_name":"Effects of Environmental Stressors on Livestock","score":0.1298000067472458,"subfield":{"id":"https://openalex.org/subfields/1103","display_name":"Animal Science and Zoology"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/heat-shock","display_name":"heat shock","score":0.9700000286102295},{"id":"https://openalex.org/keywords/apoptosis","display_name":"apoptosis","score":0.9890000224113464},{"id":"https://openalex.org/keywords/preimplantation-embryo","display_name":"preimplantation embryo","score":0.9010000228881836},{"id":"https://openalex.org/keywords/bovine-embryos","display_name":"bovine embryos","score":0.9750000238418579},{"id":"https://openalex.org/keywords/caspase-activity","display_name":"caspase activity","score":0.8659999966621399},{"id":"https://openalex.org/keywords/thermal-tolerance","display_name":"heat tolerance","score":0.7979999780654907},{"id":"https://openalex.org/keywords/developmental-stages","display_name":"developmental stages","score":0.5120000243186951}],"concepts":[{"id":"https://openalex.org/C190283241","wikidata":"https://www.wikidata.org/wiki/Q14599311","display_name":"Apoptosis","level":2,"score":0.7728254795074463},{"id":"https://openalex.org/C196843134","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryo","level":2,"score":0.6761311888694763},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6725056171417236},{"id":"https://openalex.org/C2777005246","wikidata":"https://www.wikidata.org/wiki/Q1379223","display_name":"Insemination","level":3,"score":0.611035943031311},{"id":"https://openalex.org/C16685009","wikidata":"https://www.wikidata.org/wiki/Q335057","display_name":"Andrology","level":1,"score":0.5439403653144836},{"id":"https://openalex.org/C137984847","wikidata":"https://www.wikidata.org/wiki/Q1621258","display_name":"Heat shock","level":4,"score":0.5428194999694824},{"id":"https://openalex.org/C205260736","wikidata":"https://www.wikidata.org/wiki/Q418939","display_name":"Heat shock protein","level":3,"score":0.49598297476768494},{"id":"https://openalex.org/C2781300812","wikidata":"https://www.wikidata.org/wiki/Q178061","display_name":"Shock (circulatory)","level":2,"score":0.49593648314476013},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4670737087726593},{"id":"https://openalex.org/C68991219","wikidata":"https://www.wikidata.org/wiki/Q419102","display_name":"Hsp70","level":4,"score":0.45476701855659485},{"id":"https://openalex.org/C31573885","wikidata":"https://www.wikidata.org/wiki/Q304484","display_name":"Programmed cell death","level":3,"score":0.43808242678642273},{"id":"https://openalex.org/C87073359","wikidata":"https://www.wikidata.org/wiki/Q33196","display_name":"Embryogenesis","level":3,"score":0.4343099296092987},{"id":"https://openalex.org/C145103041","wikidata":"https://www.wikidata.org/wiki/Q1151519","display_name":"Embryonic stem cell","level":3,"score":0.4160650074481964},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.32643812894821167},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.19165635108947754},{"id":"https://openalex.org/C126322002","wikidata":"https://www.wikidata.org/wiki/Q11180","display_name":"Internal medicine","level":1,"score":0.15535297989845276},{"id":"https://openalex.org/C2781087480","wikidata":"https://www.wikidata.org/wiki/Q17145","display_name":"Sperm","level":2,"score":0.1201612651348114},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.08920541405677795},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001757","descriptor_name":"Blastomeres","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D002417","descriptor_name":"Cattle","qualifier_ui":"Q000196","qualifier_name":"embryology","is_major_topic":true},{"descriptor_ui":"D002970","descriptor_name":"Cleavage Stage, Ovum","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D005260","descriptor_name":"Female","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005307","descriptor_name":"Fertilization in Vitro","qualifier_ui":"Q000662","qualifier_name":"veterinary","is_major_topic":false},{"descriptor_ui":"D006358","descriptor_name":"Hot Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D011247","descriptor_name":"Pregnancy","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013997","descriptor_name":"Time Factors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D015053","descriptor_name":"Zygote","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":false},{"descriptor_ui":"D017209","descriptor_name":"Apoptosis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true},{"descriptor_ui":"D020169","descriptor_name":"Caspases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D020287","descriptor_name":"In Situ Nick-End Labeling","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D047108","descriptor_name":"Embryonic Development","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1093/biolreprod/66.4.1169","doi":"https://doi.org/10.1093/biolreprod/66.4.1169","is_oa":false,"landing_page_url":"https://doi.org/10.1093/biolreprod/66.4.1169","pdf_url":null,"source":{"id":"https://openalex.org/S130598054","display_name":"Biology of Reproduction","issn_l":"0006-3363","issn":["0006-3363","1529-7268"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-200","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biology of Reproduction","raw_type":"journal-article"},{"id":"pmid:11906938","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/11906938","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Biology of reproduction","raw_type":"Journal Article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.980.2497","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.980.2497","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"http://www.biolreprod.org/content/66/4/1169.full.pdf","raw_type":"text"},{"id":"pmh:10.1043/0006-3363(2002)066<1169:HSIAIP>2.0.CO;2","doi":null,"is_oa":false,"landing_page_url":"http://www.bioone.org/doi/10.1043/0006-3363%282002%29066%3C1169%3AHSIAIP%3E2.0.CO%3B2","pdf_url":null,"source":{"id":"https://openalex.org/S4306402358","display_name":"BioOne Complete (BioOne)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I4210111627","host_organization_name":"BioOne","host_organization_lineage":["https://openalex.org/I4210111627"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"text"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[{"id":"https://openalex.org/F4320310009","display_name":"University of Florida","ror":"https://ror.org/02y3ad647"}],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":48,"referenced_works":["https://openalex.org/W1515506492","https://openalex.org/W1571529930","https://openalex.org/W1969158591","https://openalex.org/W1972115601","https://openalex.org/W1975378179","https://openalex.org/W2001562379","https://openalex.org/W2004886377","https://openalex.org/W2013628786","https://openalex.org/W2013751209","https://openalex.org/W2020357295","https://openalex.org/W2022176169","https://openalex.org/W2039355845","https://openalex.org/W2042992433","https://openalex.org/W2054240064","https://openalex.org/W2068688726","https://openalex.org/W2072526865","https://openalex.org/W2080737024","https://openalex.org/W2082870612","https://openalex.org/W2084736894","https://openalex.org/W2084793610","https://openalex.org/W2085114776","https://openalex.org/W2093571877","https://openalex.org/W2102390324","https://openalex.org/W2111858669","https://openalex.org/W2112985741","https://openalex.org/W2113045439","https://openalex.org/W2116574982","https://openalex.org/W2117674538","https://openalex.org/W2117875219","https://openalex.org/W2118560029","https://openalex.org/W2121670946","https://openalex.org/W2129091231","https://openalex.org/W2131754758","https://openalex.org/W2138318384","https://openalex.org/W2153985973","https://openalex.org/W2160339806","https://openalex.org/W2162756794","https://openalex.org/W2167102267","https://openalex.org/W2167962847","https://openalex.org/W2168139864","https://openalex.org/W2168601768","https://openalex.org/W2168902374","https://openalex.org/W2172252154","https://openalex.org/W2172282886","https://openalex.org/W2187271679","https://openalex.org/W2484861052","https://openalex.org/W2546782222","https://openalex.org/W4255518914"],"related_works":["https://openalex.org/W6959840092","https://openalex.org/W2057624329","https://openalex.org/W2564339412","https://openalex.org/W2019441058","https://openalex.org/W1984039592","https://openalex.org/W2155329548","https://openalex.org/W1976635787","https://openalex.org/W4402205581","https://openalex.org/W2125720158","https://openalex.org/W2113045439"],"abstract_inverted_index":{"Apoptosis":[0],"is":[1,24,69,321],"a":[2,45,91,139,290,306,322],"form":[3],"of":[4,83,107,131,145,156,166,206,239,276,281,294,311],"cell":[5],"death":[6],"that":[7,19,36,147,221,326],"can":[8,20,58,95,336],"function":[9],"to":[10,52,90,119,154,208,212,227,254,266,283,289,305,331],"eliminate":[11],"cells":[12,113,132,277],"damaged":[13],"by":[14,103,169,339],"environmental":[15],"stress.":[16],"One":[17],"stress":[18],"compromise":[21],"embryonic":[22],"development":[23],"elevated":[25,332],"temperature":[26],"(i.e.,":[27],"heat":[28,37,56,75,93,183,213,222,237,267,292,309],"shock).":[29],"For":[30],"the":[31,129,143,217,274,302],"current":[32],"studies,":[33],"we":[34],"hypothesized":[35],"shock":[38,57,94,173,184,214,223,238,293,310],"induces":[39],"apoptosis":[40,60,68,188,320],"in":[41,44,61,78,142,178,189,200,273,328],"bovine":[42,108,210],"embryos":[43,97,109,151,211,229,256,285,329],"developmentally":[46,70,323],"regulated":[47],"manner.":[48],"Studies":[49],"were":[50,148,152,230,257],"performed":[51],"1)":[53],"determine":[54],"whether":[55,66,74,88],"induce":[59,187,248],"preimplantation":[62],"embryos,":[63,193],"2)":[64],"test":[65],"heat-induced":[67,99],"regulated,":[71],"3)":[72],"evaluate":[73],"shock-induced":[76],"changes":[77],"caspase":[79,176,198],"activity":[80,177,199],"parallel":[81],"patterns":[82],"apoptosis,":[84],"and":[85,264,334],"4)":[86],"ascertain":[87],"exposure":[89,106],"mild":[92,291],"protect":[96],"from":[98],"apoptosis.":[100,134,249,279],"As":[101],"determined":[102],"TUNEL":[104],"reaction,":[105],">":[110],"or":[111,121,158,191],"=16":[112],"on":[114,232,259],"Day":[115,179,233,260,287],"5":[116,180],"after":[117,219,235,262],"insemination":[118,220,263],"41":[120,159,240,312],"42":[122],"degrees":[123,160,241,296,313],"C":[124,242,297,314],"for":[125,243,298,315],"9":[126,244,316],"h":[127,245],"increased":[128,175],"percentage":[130,275],"undergoing":[133,278],"In":[135,250,318],"addition,":[136],"there":[137,269],"was":[138,270],"duration-dependent":[140],"increase":[141,197,272],"proportion":[144],"blastomeres":[146],"apoptotic":[149,204,303],"when":[150,252],"exposed":[153,265,330],"temperatures":[155,167],"40":[157,295],"C,":[161],"which":[162],"are":[163],"more":[164],"characteristic":[165],"experienced":[168],"heat-stressed":[170],"cows.":[171],"Heat":[172],"also":[174],"embryos.":[181,202],"However,":[182],"did":[185,195,246],"not":[186,247],"2-":[190],"4-cell":[192],"nor":[194],"it":[196,335],"2-cell":[201],"The":[203],"response":[205,304],"8-":[207,226,253,282],"16-cell-stage":[209],"depended":[215],"upon":[216],"day":[218],"occurred.":[224],"When":[225],"16-cell":[228,255,284],"collected":[231,258],"3":[234],"insemination,":[236],"contrast,":[251],"4":[261,288],"shock,":[268],"an":[271],"Exposure":[280],"at":[286],"80":[299],"min":[300],"blocked":[301],"subsequent,":[307],"more-severe":[308],"h.":[317],"conclusion,":[319],"acquired":[324],"phenomenon":[325],"occurs":[327],"temperature,":[333],"be":[337],"prevented":[338],"induced":[340],"thermotolerance.":[341]},"counts_by_year":[{"year":2026,"cited_by_count":7},{"year":2025,"cited_by_count":7},{"year":2024,"cited_by_count":7},{"year":2023,"cited_by_count":6},{"year":2022,"cited_by_count":5},{"year":2021,"cited_by_count":6},{"year":2020,"cited_by_count":7},{"year":2019,"cited_by_count":6},{"year":2018,"cited_by_count":3},{"year":2017,"cited_by_count":5},{"year":2016,"cited_by_count":10},{"year":2015,"cited_by_count":10},{"year":2014,"cited_by_count":14},{"year":2013,"cited_by_count":13},{"year":2012,"cited_by_count":13}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}