{"id":"https://openalex.org/W2109012576","doi":"https://doi.org/10.1017/s1431927610000413","title":"Study of the Changes in Insoluble Polysaccharides during Pollen Development in Rice (Oryza sativa L.) by Microscopic Multispectral Imaging","display_name":"Study of the Changes in Insoluble Polysaccharides during Pollen Development in Rice (Oryza sativa L.) by Microscopic Multispectral Imaging","publication_year":2010,"publication_date":"2010-07-02","ids":{"openalex":"https://openalex.org/W2109012576","doi":"https://doi.org/10.1017/s1431927610000413","mag":"2109012576","pmid":"https://pubmed.ncbi.nlm.nih.gov/20598200"},"language":"en","primary_location":{"id":"doi:10.1017/s1431927610000413","doi":"https://doi.org/10.1017/s1431927610000413","is_oa":false,"landing_page_url":"https://doi.org/10.1017/s1431927610000413","pdf_url":null,"source":{"id":"https://openalex.org/S98208149","display_name":"Microscopy and Microanalysis","issn_l":"1431-9276","issn":["1431-9276","1435-8115"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microscopy and Microanalysis","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5011014273","display_name":"Shunan Liu","orcid":"https://orcid.org/0000-0003-0765-4579","observed_orcids":["https://orcid.org/0000-0003-0765-4579"]},"institutions":[{"id":"https://openalex.org/I37461747","display_name":"Wuhan University","ror":"https://ror.org/033vjfk17","country_code":"CN","type":"education","lineage":["https://openalex.org/I37461747"]},{"id":"https://openalex.org/I40963666","display_name":"Central China Normal University","ror":"https://ror.org/03x1jna21","country_code":"CN","type":"education","lineage":["https://openalex.org/I40963666"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Shunan Liu","raw_affiliation_strings":["The Key Laboratory of MOE for Plant Developmental Biology, Department of Genetics, College of Life Sciences, Wuhan University, Wuhan 430072, PR China","College of Life Sciences, Huazhong Normal University, Wuhan 430079, P.R. China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Key Laboratory of MOE for Plant Developmental Biology, Department of Genetics, College of Life Sciences, Wuhan University, Wuhan 430072, PR China","institution_ids":["https://openalex.org/I37461747"]},{"raw_affiliation_string":"College of Life Sciences, Huazhong Normal University, Wuhan 430079, P.R. China","institution_ids":["https://openalex.org/I40963666"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5028509203","display_name":"Lei Pan","orcid":"https://orcid.org/0000-0003-1164-0364","observed_orcids":["https://orcid.org/0000-0003-1164-0364"]},"institutions":[{"id":"https://openalex.org/I37461747","display_name":"Wuhan University","ror":"https://ror.org/033vjfk17","country_code":"CN","type":"education","lineage":["https://openalex.org/I37461747"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Lei Pan","raw_affiliation_strings":["The Key Laboratory of MOE for Plant Developmental Biology, Department of Genetics, College of Life Sciences, Wuhan University, Wuhan 430072, P.R. China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Key Laboratory of MOE for Plant Developmental Biology, Department of Genetics, College of Life Sciences, Wuhan University, Wuhan 430072, P.R. China","institution_ids":["https://openalex.org/I37461747"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100947180","display_name":"Qiongshui Wu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I37461747","display_name":"Wuhan University","ror":"https://ror.org/033vjfk17","country_code":"CN","type":"education","lineage":["https://openalex.org/I37461747"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Qiongshui Wu","raw_affiliation_strings":["Scientific Instrument Engineering Research Center, Electronic Information School, Wuhan University, Wuhan 430072, P.R. China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Scientific Instrument Engineering Research Center, Electronic Information School, Wuhan University, Wuhan 430072, P.R. China","institution_ids":["https://openalex.org/I37461747"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5089672571","display_name":"Yaojun Hu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I121296143","display_name":"Hunan University of Science and Technology","ror":"https://ror.org/02m9vrb24","country_code":"CN","type":"education","lineage":["https://openalex.org/I121296143"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Yaojun Hu","raw_affiliation_strings":["College of Life Sciences, Hunan University of Science and Technology, Xiangtan City 411201, Hunan Province, P.R. China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"College of Life Sciences, Hunan University of Science and Technology, Xiangtan City 411201, Hunan Province, P.R. China","institution_ids":["https://openalex.org/I121296143"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5100369451","display_name":"Xiaojun Chen","orcid":"https://orcid.org/0000-0002-8939-9657","observed_orcids":["https://orcid.org/0000-0002-8939-9657"]},"institutions":[{"id":"https://openalex.org/I37461747","display_name":"Wuhan University","ror":"https://ror.org/033vjfk17","country_code":"CN","type":"education","lineage":["https://openalex.org/I37461747"]}],"countries":["CN"],"is_corresponding":false,"raw_author_name":"Xiaojun Chen","raw_affiliation_strings":["The Key Laboratory of MOE for Plant Developmental Biology, Department of Genetics, College of Life Sciences, Wuhan University, Wuhan 430072, P.R. China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Key Laboratory of MOE for Plant Developmental Biology, Department of Genetics, College of Life Sciences, Wuhan University, Wuhan 430072, P.R. China","institution_ids":["https://openalex.org/I37461747"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5110204159","display_name":"Yi Ding","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I37461747","display_name":"Wuhan University","ror":"https://ror.org/033vjfk17","country_code":"CN","type":"education","lineage":["https://openalex.org/I37461747"]}],"countries":["CN"],"is_corresponding":true,"raw_author_name":"Yi Ding","raw_affiliation_strings":["The Key Laboratory of MOE for Plant Developmental Biology, Department of Genetics, College of Life Sciences, Wuhan University, Wuhan 430072, P.R. China"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"The Key Laboratory of MOE for Plant Developmental Biology, Department of Genetics, College of Life Sciences, Wuhan University, Wuhan 430072, P.R. China","institution_ids":["https://openalex.org/I37461747"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":3,"corresponding_author_ids":["https://openalex.org/A5110204159"],"corresponding_institution_ids":["https://openalex.org/I37461747"],"apc_list":{"value":3554,"currency":"USD","value_usd":3554},"apc_paid":null,"fwci":0.1062,"has_fulltext":false,"cited_by_count":2,"citation_normalized_percentile":{"value":0.40891331,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":93},"biblio":{"volume":"16","issue":"4","first_page":"488","last_page":"501"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11927","display_name":"Plant Reproductive Biology","score":0.9887999892234802,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11927","display_name":"Plant Reproductive Biology","score":0.9887999892234802,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12568","display_name":"Plant Taxonomy and Phylogenetics","score":0.0044999998062849045,"subfield":{"id":"https://openalex.org/subfields/1105","display_name":"Ecology, Evolution, Behavior and Systematics"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10869","display_name":"Polysaccharides and Plant Cell Walls","score":0.0012000000569969416,"subfield":{"id":"https://openalex.org/subfields/1110","display_name":"Plant Science"},"field":{"id":"https://openalex.org/fields/11","display_name":"Agricultural and Biological Sciences"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/insoluble-polysaccharides","display_name":"insoluble polysaccharides","score":0.972000002861023},{"id":"https://openalex.org/keywords/cytoplasmic-male-sterility","display_name":"cytoplasmic male sterility","score":0.9539999961853027},{"id":"https://openalex.org/keywords/pollen-abortion","display_name":"pollen abortion","score":0.9779999852180481},{"id":"https://openalex.org/keywords/multispectral-imaging","display_name":"multispectral imaging","score":0.6909999847412109},{"id":"https://openalex.org/keywords/periodic-acid-schiff-staining","display_name":"PAS staining","score":0.8999999761581421},{"id":"https://openalex.org/keywords/microspore-stage","display_name":"microspore stage","score":0.7049999833106995}],"concepts":[{"id":"https://openalex.org/C2780618852","wikidata":"https://www.wikidata.org/wiki/Q79932","display_name":"Pollen","level":2,"score":0.9431173801422119},{"id":"https://openalex.org/C173163844","wikidata":"https://www.wikidata.org/wiki/Q1761440","display_name":"Multispectral image","level":2,"score":0.6518478393554688},{"id":"https://openalex.org/C100817775","wikidata":"https://www.wikidata.org/wiki/Q134219","display_name":"Polysaccharide","level":2,"score":0.6474831104278564},{"id":"https://openalex.org/C2778543611","wikidata":"https://www.wikidata.org/wiki/Q161426","display_name":"Oryza sativa","level":3,"score":0.6035939455032349},{"id":"https://openalex.org/C121807389","wikidata":"https://www.wikidata.org/wiki/Q2452297","display_name":"Microspore","level":4,"score":0.5847510695457458},{"id":"https://openalex.org/C59822182","wikidata":"https://www.wikidata.org/wiki/Q441","display_name":"Botany","level":1,"score":0.5140753984451294},{"id":"https://openalex.org/C125287762","wikidata":"https://www.wikidata.org/wiki/Q1758948","display_name":"Absorption (acoustics)","level":2,"score":0.474923700094223},{"id":"https://openalex.org/C126537357","wikidata":"https://www.wikidata.org/wiki/Q372948","display_name":"Hue","level":2,"score":0.45243245363235474},{"id":"https://openalex.org/C2992726227","wikidata":"https://www.wikidata.org/wiki/Q5090","display_name":"Rice plant","level":2,"score":0.4348861575126648},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3681033253669739},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3584030866622925},{"id":"https://openalex.org/C192562407","wikidata":"https://www.wikidata.org/wiki/Q228736","display_name":"Materials science","level":0,"score":0.33191123604774475},{"id":"https://openalex.org/C29172406","wikidata":"https://www.wikidata.org/wiki/Q103129","display_name":"Stamen","level":3,"score":0.32944542169570923},{"id":"https://openalex.org/C144027150","wikidata":"https://www.wikidata.org/wiki/Q48803","display_name":"Horticulture","level":1,"score":0.32243162393569946},{"id":"https://openalex.org/C120665830","wikidata":"https://www.wikidata.org/wiki/Q14620","display_name":"Optics","level":1,"score":0.271121621131897},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.17795366048812866},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.1440279185771942},{"id":"https://openalex.org/C62649853","wikidata":"https://www.wikidata.org/wiki/Q199687","display_name":"Remote sensing","level":1,"score":0.0723201334476471},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0},{"id":"https://openalex.org/C127313418","wikidata":"https://www.wikidata.org/wiki/Q1069","display_name":"Geology","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D007091","descriptor_name":"Image Processing, Computer-Assisted","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D011058","descriptor_name":"Pollen","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D011058","descriptor_name":"Pollen","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":true},{"descriptor_ui":"D011058","descriptor_name":"Pollen","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D011134","descriptor_name":"Polysaccharides","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":true},{"descriptor_ui":"D012275","descriptor_name":"Oryza","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D012275","descriptor_name":"Oryza","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":true},{"descriptor_ui":"D012275","descriptor_name":"Oryza","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D018613","descriptor_name":"Microscopy, Confocal","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1017/s1431927610000413","doi":"https://doi.org/10.1017/s1431927610000413","is_oa":false,"landing_page_url":"https://doi.org/10.1017/s1431927610000413","pdf_url":null,"source":{"id":"https://openalex.org/S98208149","display_name":"Microscopy and Microanalysis","issn_l":"1431-9276","issn":["1431-9276","1435-8115"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310311648","host_organization_name":"Oxford University Press","host_organization_lineage":["https://openalex.org/P4310311648","https://openalex.org/P4310311647"],"host_organization_lineage_names":["Oxford University Press","University of Oxford"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microscopy and Microanalysis","raw_type":"journal-article"},{"id":"pmid:20598200","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/20598200","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[{"score":0.6875,"id":"https://metadata.un.org/sdg/2","display_name":"Zero hunger"}],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":39,"referenced_works":["https://openalex.org/W220790556","https://openalex.org/W265977097","https://openalex.org/W628853745","https://openalex.org/W1977598152","https://openalex.org/W1983061813","https://openalex.org/W1986457358","https://openalex.org/W1987253096","https://openalex.org/W1991563262","https://openalex.org/W1992775809","https://openalex.org/W1995768008","https://openalex.org/W2006225382","https://openalex.org/W2011540105","https://openalex.org/W2013537366","https://openalex.org/W2024374384","https://openalex.org/W2037479635","https://openalex.org/W2044089436","https://openalex.org/W2046628962","https://openalex.org/W2051823650","https://openalex.org/W2052342755","https://openalex.org/W2052608325","https://openalex.org/W2060786908","https://openalex.org/W2063992695","https://openalex.org/W2079749845","https://openalex.org/W2082403228","https://openalex.org/W2085765714","https://openalex.org/W2092132944","https://openalex.org/W2099295711","https://openalex.org/W2099431563","https://openalex.org/W2109875363","https://openalex.org/W2110607157","https://openalex.org/W2117803900","https://openalex.org/W2130526394","https://openalex.org/W2136795985","https://openalex.org/W2137737535","https://openalex.org/W2162664205","https://openalex.org/W2164184022","https://openalex.org/W2313694575","https://openalex.org/W2322822994","https://openalex.org/W4211173714"],"related_works":["https://openalex.org/W2052342755","https://openalex.org/W2352381981","https://openalex.org/W2364324527","https://openalex.org/W2024374384","https://openalex.org/W2313694575","https://openalex.org/W2388644729","https://openalex.org/W2529238404","https://openalex.org/W1997229252","https://openalex.org/W3151045869","https://openalex.org/W2024709404"],"abstract_inverted_index":{"Multispectral":[0],"analysis":[1],"combined":[2],"with":[3,166],"the":[4,47,53,82,85,90,96,99,108,113,118,133,158,178,193],"Periodic":[5],"Acid-Schiff":[6],"method":[7,191],"was":[8,72,87],"used":[9],"to":[10],"investigate":[11],"cytological":[12],"features":[13],"of":[14,46,55,64,81,92,102,126,150,160,195],"insoluble":[15,21],"polysaccharides":[16],"and":[17,37,52,142,187],"changes":[18,179],"in":[19,28,132,137,169,180,185],"total":[20],"polysaccharide":[22],"content":[23],"(TPC)":[24],"during":[25,141,182],"pollen":[26,57,104,120,127,144,167,183],"development":[27],"rice,":[29],"including":[30],"four":[31,97,134],"cytoplasmic":[32],"male":[33],"sterility":[34],"lines":[35,41],"(MSLs)":[36],"their":[38,138],"corresponding":[39,139],"fertility-maintaining":[40],"(FMLs).":[42],"The":[43,123],"multispectral":[44,151],"curves":[45,152],"relative":[48],"transmittance":[49],"value":[50],"(RTV)":[51],"images":[54],"developing":[56,103],"cells":[58,105,128],"were":[59,129],"obtained":[60],"across":[61],"a":[62,147,163,189],"range":[63],"successive":[65],"wavelengths":[66],"(400-720":[67],"nm).":[68],"A":[69],"minimum":[70,100],"RTV":[71,91],"found":[73],"near":[74],"550":[75,93],"nm":[76,155],"indicating":[77],"an":[78],"absorption":[79],"peak":[80],"TPC.":[83],"Thus,":[84],"TPC":[86,101,115,124,181],"measured":[88],"using":[89],"nm.":[94],"In":[95],"FMLs,":[98],"occurred":[106,116],"at":[107,117,153],"late":[109],"microspore":[110],"stage,":[111],"while":[112],"maximum":[114],"mature":[119],"grain":[121],"stage.":[122],"levels":[125],"significantly":[130],"higher":[131],"FMLs":[135],"than":[136],"MSLs":[140],"after":[143],"abortion.":[145],"Notably,":[146],"steep":[148],"decrease":[149],"420":[154],"appeared":[156],"before":[157],"occurrence":[159],"abortion,":[161],"implying":[162],"marker":[164],"associated":[165],"abortion":[168],"rice.":[170],"Our":[171],"results":[172],"will":[173],"be":[174],"helpful":[175],"for":[176,192],"exploring":[177],"ontogenesis":[184],"rice":[186],"provide":[188],"novel":[190],"study":[194],"bio-macromolecules.":[196]},"counts_by_year":[{"year":2016,"cited_by_count":1},{"year":2013,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}