{"id":"https://openalex.org/W2108960838","doi":"https://doi.org/10.3109/10606820214646","title":"Aequorin-Based Functional Assays for G-Protein-Coupled Receptors, Ion Channels, and Tyrosine Kinase Receptors","display_name":"Aequorin-Based Functional Assays for G-Protein-Coupled Receptors, Ion Channels, and Tyrosine Kinase Receptors","publication_year":2002,"publication_date":"2002-01-01","ids":{"openalex":"https://openalex.org/W2108960838","doi":"https://doi.org/10.3109/10606820214646","mag":"2108960838","pmid":"https://pubmed.ncbi.nlm.nih.gov/12690959"},"language":"en","primary_location":{"id":"doi:10.3109/10606820214646","doi":"https://doi.org/10.3109/10606820214646","is_oa":false,"landing_page_url":"https://doi.org/10.3109/10606820214646","pdf_url":null,"source":{"id":"https://openalex.org/S170807220","display_name":"Receptors and Channels","issn_l":"1060-6823","issn":["1060-6823","1543-5334","1607-856X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Receptors and Channels","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"Vincent J. Dupriez","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210113673","display_name":"Euroscreen (Belgium)","ror":"https://ror.org/01yz68468","country_code":"BE","type":"company","lineage":["https://openalex.org/I4210113673"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"Vincent J. Dupriez","raw_affiliation_strings":["Euroscreen s.a., Gosselies, Belgium"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Euroscreen s.a., Gosselies, Belgium","institution_ids":["https://openalex.org/I4210113673"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5053077971","display_name":"Karlien Maes","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210113673","display_name":"Euroscreen (Belgium)","ror":"https://ror.org/01yz68468","country_code":"BE","type":"company","lineage":["https://openalex.org/I4210113673"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"Karlien Maes","raw_affiliation_strings":["Euroscreen s.a., Gosselies, Belgium"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Euroscreen s.a., Gosselies, Belgium","institution_ids":["https://openalex.org/I4210113673"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5028801987","display_name":"Emmanuel Le Poul","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210113673","display_name":"Euroscreen (Belgium)","ror":"https://ror.org/01yz68468","country_code":"BE","type":"company","lineage":["https://openalex.org/I4210113673"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"Emmanuel Le Poul","raw_affiliation_strings":["Euroscreen s.a., Gosselies, Belgium"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Euroscreen s.a., Gosselies, Belgium","institution_ids":["https://openalex.org/I4210113673"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5037450235","display_name":"Emmanuel Burgeon","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210113673","display_name":"Euroscreen (Belgium)","ror":"https://ror.org/01yz68468","country_code":"BE","type":"company","lineage":["https://openalex.org/I4210113673"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"Emmanuel Burgeon","raw_affiliation_strings":["Euroscreen s.a., Gosselies, Belgium"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Euroscreen s.a., Gosselies, Belgium","institution_ids":["https://openalex.org/I4210113673"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5055173243","display_name":"Michel Detheux","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210113673","display_name":"Euroscreen (Belgium)","ror":"https://ror.org/01yz68468","country_code":"BE","type":"company","lineage":["https://openalex.org/I4210113673"]}],"countries":["BE"],"is_corresponding":false,"raw_author_name":"Michel Detheux","raw_affiliation_strings":["Euroscreen s.a., Gosselies, Belgium"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Euroscreen s.a., Gosselies, Belgium","institution_ids":["https://openalex.org/I4210113673"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I4210113673"],"apc_list":null,"apc_paid":null,"fwci":0.6692,"has_fulltext":false,"cited_by_count":63,"citation_normalized_percentile":{"value":0.63572775,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":97},"biblio":{"volume":"8","issue":"5-6","first_page":"319","last_page":"330"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T12561","display_name":"bioluminescence and chemiluminescence research","score":0.9916999936103821,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T12561","display_name":"bioluminescence and chemiluminescence research","score":0.9916999936103821,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11178","display_name":"Receptor Mechanisms and Signaling","score":0.006399999838322401,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.0003000000142492354,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/aequorin","display_name":"aequorin","score":1.0},{"id":"https://openalex.org/keywords/g-protein-coupled-receptors","display_name":"G protein-coupled receptors","score":0.843999981880188},{"id":"https://openalex.org/keywords/ion-channels","display_name":"ion channels","score":1.0},{"id":"https://openalex.org/keywords/receptor-tyrosine-kinases","display_name":"receptor tyrosine kinase","score":0.9980000257492065},{"id":"https://openalex.org/keywords/intracellular-calcium-imaging","display_name":"intracellular calcium imaging","score":0.7139999866485596},{"id":"https://openalex.org/keywords/high-throughput-screening","display_name":"high-throughput screening","score":0.6790000200271606},{"id":"https://openalex.org/keywords/pharmacological-characterization","display_name":"pharmacological characterization","score":0.7319999933242798}],"concepts":[{"id":"https://openalex.org/C80933277","wikidata":"https://www.wikidata.org/wiki/Q381068","display_name":"Aequorin","level":3,"score":0.9887871742248535},{"id":"https://openalex.org/C17189327","wikidata":"https://www.wikidata.org/wiki/Q7187889","display_name":"Photoprotein","level":3,"score":0.616856038570404},{"id":"https://openalex.org/C170493617","wikidata":"https://www.wikidata.org/wiki/Q208467","display_name":"Receptor","level":2,"score":0.5491790771484375},{"id":"https://openalex.org/C5176359","wikidata":"https://www.wikidata.org/wiki/Q179924","display_name":"Bioluminescence","level":2,"score":0.5214309692382812},{"id":"https://openalex.org/C135285700","wikidata":"https://www.wikidata.org/wiki/Q38173","display_name":"G protein-coupled receptor","level":3,"score":0.5116908550262451},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.4326649308204651},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4276406168937683},{"id":"https://openalex.org/C2778938600","wikidata":"https://www.wikidata.org/wiki/Q389934","display_name":"Agonist","level":3,"score":0.4259307384490967},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.42252418398857117},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.3994327485561371},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.36717745661735535},{"id":"https://openalex.org/C79879829","wikidata":"https://www.wikidata.org/wiki/Q5571762","display_name":"Intracellular","level":2,"score":0.2326831817626953}],"mesh":[{"descriptor_ui":"D000331","descriptor_name":"Aequorin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D000331","descriptor_name":"Aequorin","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001671","descriptor_name":"Biochemistry","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D001709","descriptor_name":"Biotechnology","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":true},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002851","descriptor_name":"Chromatography, High Pressure Liquid","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003593","descriptor_name":"Cytoplasm","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D004305","descriptor_name":"Dose-Response Relationship, Drug","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004815","descriptor_name":"Epidermal Growth Factor","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D006224","descriptor_name":"Cricetinae","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007473","descriptor_name":"Ion Channels","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D007473","descriptor_name":"Ion Channels","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D007477","descriptor_name":"Ions","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D007700","descriptor_name":"Kinetics","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008928","descriptor_name":"Mitochondria","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010920","descriptor_name":"Placenta","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011485","descriptor_name":"Protein Binding","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011505","descriptor_name":"Protein-Tyrosine Kinases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011955","descriptor_name":"Receptors, Drug","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011956","descriptor_name":"Receptors, Cell Surface","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D013997","descriptor_name":"Time Factors","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D016466","descriptor_name":"CHO Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D019204","descriptor_name":"GTP-Binding Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D019556","descriptor_name":"COS Cells","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D020794","descriptor_name":"Receptor Protein-Tyrosine Kinases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.3109/10606820214646","doi":"https://doi.org/10.3109/10606820214646","is_oa":false,"landing_page_url":"https://doi.org/10.3109/10606820214646","pdf_url":null,"source":{"id":"https://openalex.org/S170807220","display_name":"Receptors and Channels","issn_l":"1060-6823","issn":["1060-6823","1543-5334","1607-856X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Receptors and Channels","raw_type":"journal-article"},{"id":"doi:10.1080/10606820214646","doi":"https://doi.org/10.1080/10606820214646","is_oa":false,"landing_page_url":"https://doi.org/10.1080/10606820214646","pdf_url":null,"source":{"id":"https://openalex.org/S170807220","display_name":"Receptors and Channels","issn_l":"1060-6823","issn":["1060-6823","1543-5334","1607-856X"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core"],"host_organization":"https://openalex.org/P4310320547","host_organization_name":"Taylor & Francis","host_organization_lineage":["https://openalex.org/P4310320547","https://openalex.org/P4310320449"],"host_organization_lineage_names":["Taylor & Francis","Informa"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Receptors and Channels","raw_type":"journal-article"},{"id":"pmid:12690959","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/12690959","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Receptors & channels","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":34,"referenced_works":["https://openalex.org/W20155870","https://openalex.org/W1417397851","https://openalex.org/W1482828226","https://openalex.org/W1501048482","https://openalex.org/W1511198581","https://openalex.org/W1595694860","https://openalex.org/W1735255634","https://openalex.org/W1877788794","https://openalex.org/W1939705865","https://openalex.org/W1963791094","https://openalex.org/W1966112698","https://openalex.org/W1983659533","https://openalex.org/W1984482377","https://openalex.org/W1985147717","https://openalex.org/W1987800116","https://openalex.org/W1991426989","https://openalex.org/W1992876375","https://openalex.org/W1998329643","https://openalex.org/W2001402428","https://openalex.org/W2009301053","https://openalex.org/W2012912004","https://openalex.org/W2028007732","https://openalex.org/W2032781637","https://openalex.org/W2035980989","https://openalex.org/W2046353863","https://openalex.org/W2052639580","https://openalex.org/W2058839845","https://openalex.org/W2064534986","https://openalex.org/W2090091116","https://openalex.org/W2111338104","https://openalex.org/W2117501535","https://openalex.org/W2153630912","https://openalex.org/W2163456630","https://openalex.org/W2261726105"],"related_works":["https://openalex.org/W2040796207","https://openalex.org/W2093247393","https://openalex.org/W2093474650","https://openalex.org/W2010442956","https://openalex.org/W2082216078","https://openalex.org/W2914169447","https://openalex.org/W2171643160","https://openalex.org/W1417397851","https://openalex.org/W2068894903","https://openalex.org/W2019257137"],"abstract_inverted_index":{"Aequorin":[0],"is":[1,10],"a":[2,101],"photoprotein":[3],"originating":[4],"from":[5],"jellyfish,":[6],"whose":[7],"luminescent":[8,28],"activity":[9],"dependent":[11],"on":[12],"the":[13,20,57,78,81,96,105,138,163,166],"concentration":[14],"of":[15,36,63,72,84,98,107,158,162,169],"calcium":[16,51],"ions.":[17],"Due":[18],"to":[19,27,33,80,176],"high":[21,180],"sensitivity":[22],"and":[23,38,112,125,135,151,165],"low":[24],"background":[25],"linked":[26],"assays,":[29],"as":[30,32,48,100,116,118],"well":[31,117],"its":[34,39,54,66,73],"absence":[35],"toxicity":[37],"large":[40],"linear":[41],"dynamic":[42],"range,":[43],"aequorin":[44,64,85,99,150,175],"has":[45],"been":[46],"used":[47],"an":[49],"intracellular":[50],"indicator":[52],"since":[53],"discovery":[55],"in":[56,68,75,86,123],"early":[58],"1960s.":[59],"The":[60,70,160],"first":[61],"applications":[62],"involved":[65],"microinjection":[67],"cells.":[69],"cloning":[71],"gene":[74],"1985":[76],"opened":[77],"way":[79],"stable":[82],"expression":[83],"cell":[87,132],"lines":[88],"or":[89],"even":[90],"entire":[91],"organisms.":[92],"Here":[93],"we":[94],"present":[95],"validation":[97],"functional":[102,182],"assay":[103,134,141,164],"for":[104,119,156],"screening":[106],"G-protein-coupled":[108],"receptors,":[109,115],"ion":[110],"channels,":[111],"tyrosine":[113],"kinase":[114],"their":[120],"pharmacological":[121],"characterization":[122],"agonist":[124],"antagonist":[126],"detection":[127],"assays.":[128],"We":[129],"optimized":[130],"our":[131],"suspension-based":[133],"determined":[136],"that":[137],"most":[139],"sensitive":[140],"was":[142],"performed":[143],"at":[144],"room":[145],"temperature,":[146],"with":[147,171,179],"mitochondrially":[148],"expressed":[149],"using":[152],"coelenterazine":[153],"derivative":[154],"h":[155],"reconstitution":[157],"aequorin.":[159],"robustness":[161],"current":[167],"availability":[168],"luminometers":[170],"integrated":[172],"injectors":[173],"allow":[174],"fit":[177],"perfectly":[178],"throughput":[181],"assays":[183],"requirements.":[184]},"counts_by_year":[{"year":2023,"cited_by_count":1},{"year":2022,"cited_by_count":1},{"year":2021,"cited_by_count":5},{"year":2020,"cited_by_count":2},{"year":2019,"cited_by_count":3},{"year":2018,"cited_by_count":1},{"year":2017,"cited_by_count":2},{"year":2015,"cited_by_count":4},{"year":2014,"cited_by_count":2},{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-08T12:12:28.789561","created_date":"2016-06-24T00:00:00"}
