{"id":"https://openalex.org/W2108180141","doi":"https://doi.org/10.1002/0471140856.tx2202s47","title":"Isolation and Applications of Prostate Side Population Cells Based on Dye Cycle Violet Efflux","display_name":"Isolation and Applications of Prostate Side Population Cells Based on Dye Cycle Violet Efflux","publication_year":2011,"publication_date":"2011-02-01","ids":{"openalex":"https://openalex.org/W2108180141","doi":"https://doi.org/10.1002/0471140856.tx2202s47","mag":"2108180141","pmid":"https://pubmed.ncbi.nlm.nih.gov/21400686","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/3058348"},"language":"en","primary_location":{"id":"doi:10.1002/0471140856.tx2202s47","doi":"https://doi.org/10.1002/0471140856.tx2202s47","is_oa":false,"landing_page_url":"https://doi.org/10.1002/0471140856.tx2202s47","pdf_url":null,"source":{"id":"https://openalex.org/S4210173016","display_name":"Current Protocols in Toxicology","issn_l":"1934-9254","issn":["1934-9254","1934-9262"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-40"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Current Protocols in Toxicology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3058348","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5026058598","display_name":"Kalyan J. Gangavarapu","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I1292894508","display_name":"Roswell Park Comprehensive Cancer Center","ror":"https://ror.org/0499dwk57","country_code":"US","type":"facility","lineage":["https://openalex.org/I1292894508"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kalyan J. Gangavarapu","raw_affiliation_strings":["Roswell Park Cancer Institute  Buffalo New York"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Roswell Park Cancer Institute  Buffalo New York","institution_ids":["https://openalex.org/I1292894508"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5067861660","display_name":"Wendy J. Huss","orcid":"https://orcid.org/0000-0002-0812-8696","observed_orcids":["https://orcid.org/0000-0002-0812-8696"]},"institutions":[{"id":"https://openalex.org/I1292894508","display_name":"Roswell Park Comprehensive Cancer Center","ror":"https://ror.org/0499dwk57","country_code":"US","type":"facility","lineage":["https://openalex.org/I1292894508"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Wendy J. Huss","raw_affiliation_strings":["Roswell Park Cancer Institute  Buffalo New York"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Roswell Park Cancer Institute  Buffalo New York","institution_ids":["https://openalex.org/I1292894508"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I1292894508"],"apc_list":{"value":5050,"currency":"USD","value_usd":5050},"apc_paid":null,"fwci":1.0881,"has_fulltext":false,"cited_by_count":15,"citation_normalized_percentile":{"value":0.80873426,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"47","issue":"1","first_page":"Unit 22.2","last_page":"Unit 22.2"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10543","display_name":"Prostate Cancer Treatment and Research","score":0.5288000106811523,"subfield":{"id":"https://openalex.org/subfields/2740","display_name":"Pulmonary and Respiratory Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10543","display_name":"Prostate Cancer Treatment and Research","score":0.5288000106811523,"subfield":{"id":"https://openalex.org/subfields/2740","display_name":"Pulmonary and Respiratory Medicine"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10570","display_name":"Drug Transport and Resistance Mechanisms","score":0.4113999903202057,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T10336","display_name":"Cancer Cells and Metastasis","score":0.03229999914765358,"subfield":{"id":"https://openalex.org/subfields/2730","display_name":"Oncology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/abc-transporters","display_name":"ABC transporters","score":0.8560000061988831},{"id":"https://openalex.org/keywords/tissue-digestion","display_name":"tissue digestion","score":0.7850000262260437},{"id":"https://openalex.org/keywords/flow-cytometry-cell-sorting","display_name":"flow cytometric cell sorting","score":0.5920000076293945},{"id":"https://openalex.org/keywords/stem-cells","display_name":"stem cells","score":0.3700000047683716}],"concepts":[{"id":"https://openalex.org/C2780546549","wikidata":"https://www.wikidata.org/wiki/Q7508338","display_name":"Side population","level":4,"score":0.847244143486023},{"id":"https://openalex.org/C200082930","wikidata":"https://www.wikidata.org/wiki/Q22327148","display_name":"Efflux","level":2,"score":0.7210476398468018},{"id":"https://openalex.org/C2908647359","wikidata":"https://www.wikidata.org/wiki/Q2625603","display_name":"Population","level":2,"score":0.6402950286865234},{"id":"https://openalex.org/C553184892","wikidata":"https://www.wikidata.org/wiki/Q1141429","display_name":"Flow cytometry","level":2,"score":0.6215327978134155},{"id":"https://openalex.org/C74864618","wikidata":"https://www.wikidata.org/wiki/Q2332446","display_name":"Staining","level":2,"score":0.6160156726837158},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5020225048065186},{"id":"https://openalex.org/C2778630268","wikidata":"https://www.wikidata.org/wiki/Q17113277","display_name":"Cell sorting","level":3,"score":0.4216015934944153},{"id":"https://openalex.org/C149011108","wikidata":"https://www.wikidata.org/wiki/Q652985","display_name":"Transporter","level":3,"score":0.4174196720123291},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.4137043058872223},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.36856240034103394},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.36191999912261963},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.3127913177013397},{"id":"https://openalex.org/C71924100","wikidata":"https://www.wikidata.org/wiki/Q11190","display_name":"Medicine","level":0,"score":0.10561719536781311},{"id":"https://openalex.org/C127716648","wikidata":"https://www.wikidata.org/wiki/Q104053","display_name":"Phenotype","level":3,"score":0.078229159116745},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.068550705909729},{"id":"https://openalex.org/C99454951","wikidata":"https://www.wikidata.org/wiki/Q932068","display_name":"Environmental health","level":1,"score":0.0},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.0}],"mesh":[{"descriptor_ui":"D002469","descriptor_name":"Cell Separation","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D005434","descriptor_name":"Flow Cytometry","qualifier_ui":"Q000379","qualifier_name":"methods","is_major_topic":false},{"descriptor_ui":"D005456","descriptor_name":"Fluorescent Dyes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008297","descriptor_name":"Male","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011467","descriptor_name":"Prostate","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D013196","descriptor_name":"Dihydrotestosterone","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D013196","descriptor_name":"Dihydrotestosterone","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D014316","descriptor_name":"Tritium","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014316","descriptor_name":"Tritium","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D058985","descriptor_name":"Side-Population Cells","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D058985","descriptor_name":"Side-Population Cells","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false}],"locations_count":3,"locations":[{"id":"doi:10.1002/0471140856.tx2202s47","doi":"https://doi.org/10.1002/0471140856.tx2202s47","is_oa":false,"landing_page_url":"https://doi.org/10.1002/0471140856.tx2202s47","pdf_url":null,"source":{"id":"https://openalex.org/S4210173016","display_name":"Current Protocols in Toxicology","issn_l":"1934-9254","issn":["1934-9254","1934-9262"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","poland-40"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Current Protocols in Toxicology","raw_type":"journal-article"},{"id":"pmid:21400686","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/21400686","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Current protocols in toxicology","raw_type":"Journal Article"},{"id":"pmh:oai:pubmedcentral.nih.gov:3058348","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3058348","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Curr Protoc Toxicol","raw_type":"Text"}],"best_oa_location":{"id":"pmh:oai:pubmedcentral.nih.gov:3058348","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/3058348","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"acceptedVersion","is_accepted":true,"is_published":false,"raw_source_name":"Curr Protoc Toxicol","raw_type":"Text"},"sustainable_development_goals":[{"score":0.8234000205993652,"display_name":"Good health and well-being","id":"https://metadata.un.org/sdg/3"}],"sustainable_development_goals_aurora":[{"score":0.46000000834465027,"display_name":"Clean water and sanitation","id":"https://metadata.un.org/sdg/6"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G3928915630","display_name":"Differentiation Signaling in Prostate Stem Cells","funder_award_id":"R01DK091240","funder_id":"https://openalex.org/F4320337357","funder_display_name":"National Institute of Diabetes and Digestive and Kidney Diseases"},{"id":"https://openalex.org/G9000888465","display_name":"Roswell Park Cancer Center Support Grant","funder_award_id":"P30CA016056","funder_id":"https://openalex.org/F4320337351","funder_display_name":"National Cancer Institute"}],"funders":[{"id":"https://openalex.org/F4320337351","display_name":"National Cancer Institute","ror":"https://ror.org/040gcmg81"},{"id":"https://openalex.org/F4320337357","display_name":"National Institute of Diabetes and Digestive and Kidney Diseases","ror":"https://ror.org/00adh9b73"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":30,"referenced_works":["https://openalex.org/W1483167997","https://openalex.org/W1546006749","https://openalex.org/W1879626379","https://openalex.org/W1909893903","https://openalex.org/W1964044777","https://openalex.org/W1965283402","https://openalex.org/W1972764534","https://openalex.org/W1973541913","https://openalex.org/W1983636976","https://openalex.org/W1990361620","https://openalex.org/W1995275527","https://openalex.org/W2000986783","https://openalex.org/W2011994267","https://openalex.org/W2014189145","https://openalex.org/W2022549451","https://openalex.org/W2022881840","https://openalex.org/W2027258358","https://openalex.org/W2045477445","https://openalex.org/W2062069198","https://openalex.org/W2072450847","https://openalex.org/W2112197935","https://openalex.org/W2125519401","https://openalex.org/W2137956382","https://openalex.org/W2149860336","https://openalex.org/W2150485214","https://openalex.org/W2155884637","https://openalex.org/W2159264387","https://openalex.org/W2165206392","https://openalex.org/W2167960944","https://openalex.org/W2415222803"],"related_works":["https://openalex.org/W1964044777","https://openalex.org/W4251448620","https://openalex.org/W1977745477","https://openalex.org/W2517296648","https://openalex.org/W7213979998","https://openalex.org/W2165206392","https://openalex.org/W2531240456","https://openalex.org/W2078321432","https://openalex.org/W2605325367","https://openalex.org/W2159264387"],"abstract_inverted_index":{"This":[0,84],"unit":[1,85],"describes":[2],"methods":[3],"for":[4,17,93],"digestion":[5],"of":[6,22,27,33,43,74,100],"human":[7],"prostate":[8],"clinical":[9],"specimens":[10],"and":[11,20,67,98],"dye":[12],"cycle":[13],"violet":[14],"(DCV)":[15],"staining":[16],"identification,":[18],"isolation,":[19],"quantitation":[21,99],"radiolabeled":[23,101],"dihydrotestosterone":[24],"(DHT)":[25],"retention":[26],"side":[28,35,95],"population":[29,36,96],"cells.":[30,83],"The":[31],"principle":[32],"the":[34,70],"assay":[37],"is":[38],"based":[39],"on":[40],"differential":[41],"efflux":[42,65],"DCV,":[44],"a":[45,75],"cell-membrane-permeable":[46],"fluorescent":[47],"dye,":[48],"by":[49],"cells":[50],"with":[51,59],"high":[52,60],"ATP-binding":[53],"cassette":[54],"(ABC)":[55],"transporter":[56,62],"activity.":[57],"Cells":[58],"ABC":[61],"activity":[63],"that":[64],"DCV":[66,89],"fall":[68],"in":[69],"lower":[71],"left":[72],"quadrant":[73],"flow":[76,91],"cytograph":[77],"are":[78],"designated":[79],"as":[80],"\"side":[81],"population\"":[82],"emphasizes":[86],"tissue":[87],"digestion,":[88],"staining,":[90],"settings":[92],"sorting":[94],"cells,":[97],"DHT":[102],"retention.":[103]},"counts_by_year":[{"year":2021,"cited_by_count":4},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":1},{"year":2016,"cited_by_count":1},{"year":2015,"cited_by_count":2},{"year":2013,"cited_by_count":4}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}