{"id":"https://openalex.org/W2107374367","doi":"https://doi.org/10.1128/aem.00989-14","title":"Nonperturbative Imaging of Nucleoid Morphology in Live Bacterial Cells during an Antimicrobial Peptide Attack","display_name":"Nonperturbative Imaging of Nucleoid Morphology in Live Bacterial Cells during an Antimicrobial Peptide Attack","publication_year":2014,"publication_date":"2014-06-07","ids":{"openalex":"https://openalex.org/W2107374367","doi":"https://doi.org/10.1128/aem.00989-14","mag":"2107374367","pmid":"https://pubmed.ncbi.nlm.nih.gov/24907320","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/4135745"},"language":"en","primary_location":{"id":"doi:10.1128/aem.00989-14","doi":"https://doi.org/10.1128/aem.00989-14","is_oa":true,"landing_page_url":"https://doi.org/10.1128/aem.00989-14","pdf_url":"https://aem.asm.org/content/aem/80/16/4977.full.pdf","source":{"id":"https://openalex.org/S158228368","display_name":"Applied and Environmental Microbiology","issn_l":"0099-2240","issn":["0099-2240","1070-6291","1098-5336"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and Environmental Microbiology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"bronze","oa_url":"https://aem.asm.org/content/aem/80/16/4977.full.pdf","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5014669169","display_name":"Somenath Bakshi","orcid":"https://orcid.org/0000-0003-3864-2546","observed_orcids":["https://orcid.org/0000-0003-3864-2546"]},"institutions":[{"id":"https://openalex.org/I135310074","display_name":"University of Wisconsin\u2013Madison","ror":"https://ror.org/01y2jtd41","country_code":"US","type":"education","lineage":["https://openalex.org/I135310074"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Somenath Bakshi","raw_affiliation_strings":["Department of Chemistry, University of Wisconsin\u2014Madison, Madison, Wisconsin, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of Wisconsin\u2014Madison, Madison, Wisconsin, USA","institution_ids":["https://openalex.org/I135310074"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5053001708","display_name":"Heejun Choi","orcid":"https://orcid.org/0000-0002-1337-3813","observed_orcids":["https://orcid.org/0000-0002-1337-3813","https://orcid.org/0000-0003-2136-9598"]},"institutions":[{"id":"https://openalex.org/I135310074","display_name":"University of Wisconsin\u2013Madison","ror":"https://ror.org/01y2jtd41","country_code":"US","type":"education","lineage":["https://openalex.org/I135310074"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Heejun Choi","raw_affiliation_strings":["Department of Chemistry, University of Wisconsin\u2014Madison, Madison, Wisconsin, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of Wisconsin\u2014Madison, Madison, Wisconsin, USA","institution_ids":["https://openalex.org/I135310074"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5036992229","display_name":"Nambirajan Rangarajan","orcid":"https://orcid.org/0000-0002-8332-2448","observed_orcids":["https://orcid.org/0000-0002-8332-2448"]},"institutions":[{"id":"https://openalex.org/I135310074","display_name":"University of Wisconsin\u2013Madison","ror":"https://ror.org/01y2jtd41","country_code":"US","type":"education","lineage":["https://openalex.org/I135310074"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Nambirajan Rangarajan","raw_affiliation_strings":["Department of Chemistry, University of Wisconsin\u2014Madison, Madison, Wisconsin, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of Wisconsin\u2014Madison, Madison, Wisconsin, USA","institution_ids":["https://openalex.org/I135310074"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5086348253","display_name":"Kenneth J. Barns","orcid":"https://orcid.org/0000-0002-1856-4280","observed_orcids":["https://orcid.org/0000-0002-1856-4280"]},"institutions":[{"id":"https://openalex.org/I135310074","display_name":"University of Wisconsin\u2013Madison","ror":"https://ror.org/01y2jtd41","country_code":"US","type":"education","lineage":["https://openalex.org/I135310074"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Kenneth J. Barns","raw_affiliation_strings":["Department of Chemistry, University of Wisconsin\u2014Madison, Madison, Wisconsin, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of Wisconsin\u2014Madison, Madison, Wisconsin, USA","institution_ids":["https://openalex.org/I135310074"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5036984709","display_name":"Benjamin P. Bratton","orcid":"https://orcid.org/0000-0003-1128-2560","observed_orcids":["https://orcid.org/0000-0003-1128-2560"]},"institutions":[{"id":"https://openalex.org/I135310074","display_name":"University of Wisconsin\u2013Madison","ror":"https://ror.org/01y2jtd41","country_code":"US","type":"education","lineage":["https://openalex.org/I135310074"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Benjamin P. Bratton","raw_affiliation_strings":["Department of Chemistry, University of Wisconsin\u2014Madison, Madison, Wisconsin, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of Wisconsin\u2014Madison, Madison, Wisconsin, USA","institution_ids":["https://openalex.org/I135310074"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5077564904","display_name":"James C. Weisshaar","orcid":"https://orcid.org/0000-0002-0922-5113","observed_orcids":["https://orcid.org/0000-0002-0922-5113"]},"institutions":[{"id":"https://openalex.org/I135310074","display_name":"University of Wisconsin\u2013Madison","ror":"https://ror.org/01y2jtd41","country_code":"US","type":"education","lineage":["https://openalex.org/I135310074"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"James C. Weisshaar","raw_affiliation_strings":["Department of Chemistry, University of Wisconsin\u2014Madison, Madison, Wisconsin, USA","Molecular Biophysics Program, University of Wisconsin\u2014Madison, Madison, Wisconsin, USA"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Chemistry, University of Wisconsin\u2014Madison, Madison, Wisconsin, USA","institution_ids":["https://openalex.org/I135310074"]},{"raw_affiliation_string":"Molecular Biophysics Program, University of Wisconsin\u2014Madison, Madison, Wisconsin, USA","institution_ids":["https://openalex.org/I135310074"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I135310074"],"apc_list":null,"apc_paid":null,"fwci":4.1096,"has_fulltext":false,"cited_by_count":58,"citation_normalized_percentile":{"value":0.94456315,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":93,"max":99},"biblio":{"volume":"80","issue":"16","first_page":"4977","last_page":"4986"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.6035000085830688,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.6035000085830688,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10540","display_name":"Advanced Fluorescence Microscopy Techniques","score":0.2328999936580658,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12859","display_name":"Cell Image Analysis Techniques","score":0.050599999725818634,"subfield":{"id":"https://openalex.org/subfields/1304","display_name":"Biophysics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/sytox-green","display_name":"SYTOX Green","score":0.9629999995231628},{"id":"https://openalex.org/keywords/draq5","display_name":"DRAQ5","score":0.9950000047683716},{"id":"https://openalex.org/keywords/dapi","display_name":"DAPI","score":0.9290000200271606},{"id":"https://openalex.org/keywords/escherichia-coli","display_name":"Escherichia coli","score":0.925000011920929},{"id":"https://openalex.org/keywords/bacillus-subtilis","display_name":"Bacillus subtilis","score":1.0},{"id":"https://openalex.org/keywords/antimicrobial-peptides","display_name":"antimicrobial peptides","score":0.9259999990463257},{"id":"https://openalex.org/keywords/cecropin-a","display_name":"cecropin A","score":0.9990000128746033},{"id":"https://openalex.org/keywords/ll-37","display_name":"LL-37","score":0.9559999704360962},{"id":"https://openalex.org/keywords/cell-permeabilization","display_name":"cell permeabilization","score":0.7129999995231628},{"id":"https://openalex.org/keywords/rifampicin","display_name":"rifampicin","score":0.5370000004768372},{"id":"https://openalex.org/keywords/single-cell-fluorescence-imaging","display_name":"single-cell fluorescence imaging","score":0.5559999942779541},{"id":"https://openalex.org/keywords/emccd-camera","display_name":"EMCCD camera","score":0.9210000038146973}],"concepts":[{"id":"https://openalex.org/C53642103","wikidata":"https://www.wikidata.org/wiki/Q459485","display_name":"Nucleoid","level":4,"score":0.8983546495437622},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6737564206123352},{"id":"https://openalex.org/C2777527632","wikidata":"https://www.wikidata.org/wiki/Q238382","display_name":"DAPI","level":3,"score":0.6715509295463562},{"id":"https://openalex.org/C2777272437","wikidata":"https://www.wikidata.org/wiki/Q131238","display_name":"Bacillus subtilis","level":3,"score":0.5064207315444946},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.4718320965766907},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4547112286090851},{"id":"https://openalex.org/C15526883","wikidata":"https://www.wikidata.org/wiki/Q6657126","display_name":"Live cell imaging","level":3,"score":0.45058318972587585},{"id":"https://openalex.org/C169274487","wikidata":"https://www.wikidata.org/wiki/Q840211","display_name":"Fluorescence microscope","level":3,"score":0.4388972222805023},{"id":"https://openalex.org/C99054511","wikidata":"https://www.wikidata.org/wiki/Q2630359","display_name":"Spheroplast","level":4,"score":0.42549705505371094},{"id":"https://openalex.org/C88478588","wikidata":"https://www.wikidata.org/wiki/Q42244","display_name":"Ribosome","level":4,"score":0.41703474521636963},{"id":"https://openalex.org/C91881484","wikidata":"https://www.wikidata.org/wiki/Q191807","display_name":"Fluorescence","level":2,"score":0.38501444458961487},{"id":"https://openalex.org/C1491633281","wikidata":"https://www.wikidata.org/wiki/Q7868","display_name":"Cell","level":2,"score":0.35519587993621826},{"id":"https://openalex.org/C547475151","wikidata":"https://www.wikidata.org/wiki/Q25419","display_name":"Escherichia coli","level":3,"score":0.3175045847892761},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.2540241479873657},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.1860916018486023},{"id":"https://openalex.org/C190283241","wikidata":"https://www.wikidata.org/wiki/Q14599311","display_name":"Apoptosis","level":2,"score":0.1314851939678192},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.09266665577888489},{"id":"https://openalex.org/C62520636","wikidata":"https://www.wikidata.org/wiki/Q944","display_name":"Quantum mechanics","level":1,"score":0.0},{"id":"https://openalex.org/C121332964","wikidata":"https://www.wikidata.org/wiki/Q413","display_name":"Physics","level":0,"score":0.0},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.0},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D001412","descriptor_name":"Bacillus subtilis","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D001412","descriptor_name":"Bacillus subtilis","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D001412","descriptor_name":"Bacillus subtilis","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":false},{"descriptor_ui":"D001412","descriptor_name":"Bacillus subtilis","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D002466","descriptor_name":"Cell Nucleolus","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D002466","descriptor_name":"Cell Nucleolus","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000166","qualifier_name":"cytology","is_major_topic":true},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":true},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000254","qualifier_name":"growth & development","is_major_topic":false},{"descriptor_ui":"D005456","descriptor_name":"Fluorescent Dyes","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D006801","descriptor_name":"Humans","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008856","descriptor_name":"Microscopy, Fluorescence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D013194","descriptor_name":"Staining and Labeling","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D023181","descriptor_name":"Antimicrobial Cationic Peptides","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D054804","descriptor_name":"Cathelicidins","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":4,"locations":[{"id":"doi:10.1128/aem.00989-14","doi":"https://doi.org/10.1128/aem.00989-14","is_oa":true,"landing_page_url":"https://doi.org/10.1128/aem.00989-14","pdf_url":"https://aem.asm.org/content/aem/80/16/4977.full.pdf","source":{"id":"https://openalex.org/S158228368","display_name":"Applied and Environmental Microbiology","issn_l":"0099-2240","issn":["0099-2240","1070-6291","1098-5336"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and Environmental Microbiology","raw_type":"journal-article"},{"id":"pmh:oai:pubmedcentral.nih.gov:4135745","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/4135745","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Appl Environ Microbiol","raw_type":"Text"},{"id":"pmid:24907320","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/24907320","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and environmental microbiology","raw_type":"Journal Article"},{"id":"pmh:oai:generic.eprints.org:1195018","doi":null,"is_oa":false,"landing_page_url":"http://publications.eng.cam.ac.uk/1195018/","pdf_url":null,"source":{"id":"https://openalex.org/S4406922847","display_name":"Cambridge University Engineering Department Publications Database","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":null,"host_organization_name":null,"host_organization_lineage":[],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"","raw_type":"Article"}],"best_oa_location":{"id":"doi:10.1128/aem.00989-14","doi":"https://doi.org/10.1128/aem.00989-14","is_oa":true,"landing_page_url":"https://doi.org/10.1128/aem.00989-14","pdf_url":"https://aem.asm.org/content/aem/80/16/4977.full.pdf","source":{"id":"https://openalex.org/S158228368","display_name":"Applied and Environmental Microbiology","issn_l":"0099-2240","issn":["0099-2240","1070-6291","1098-5336"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["anvur-scientific","cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-2","poland-100","russia-white-list-1","vabb-shw"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied and Environmental Microbiology","raw_type":"journal-article"},"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.4000000059604645,"id":"https://metadata.un.org/sdg/3"}],"study_designs":[],"awards":[{"id":"https://openalex.org/G7948752355","display_name":"Dynamics of Antimicrobial Peptide Interactions with Bacterial Membranes","funder_award_id":"R01GM094510","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"},{"id":"https://openalex.org/G8480515937","display_name":"Design and Analysis of Random Copolymers with Antimicrobial Activity","funder_award_id":"R01GM093265","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"},{"id":"https://openalex.org/F4320337393","display_name":"Division of Chemistry","ror":"https://ror.org/01ar8dr59"}],"has_content":{"pdf":true,"grobid_xml":false},"content_urls":{"pdf":"https://content.openalex.org/works/W2107374367.pdf"},"referenced_works_count":23,"referenced_works":["https://openalex.org/W1781531090","https://openalex.org/W1883779479","https://openalex.org/W1943854657","https://openalex.org/W1975497257","https://openalex.org/W1978669792","https://openalex.org/W1990823004","https://openalex.org/W2001162607","https://openalex.org/W2011456814","https://openalex.org/W2025230440","https://openalex.org/W2026204622","https://openalex.org/W2046796256","https://openalex.org/W2060177007","https://openalex.org/W2065927326","https://openalex.org/W2076871519","https://openalex.org/W2078113814","https://openalex.org/W2089065960","https://openalex.org/W2099412396","https://openalex.org/W2124983475","https://openalex.org/W2136955543","https://openalex.org/W2141849008","https://openalex.org/W2143587535","https://openalex.org/W2155463008","https://openalex.org/W2167302836"],"related_works":["https://openalex.org/W2143587535","https://openalex.org/W4411992550","https://openalex.org/W2167302836","https://openalex.org/W2315524682","https://openalex.org/W2044262524","https://openalex.org/W2755234167","https://openalex.org/W2072024709","https://openalex.org/W7172525910","https://openalex.org/W2769773184","https://openalex.org/W2085436481"],"abstract_inverted_index":{"Studies":[0],"of":[1,48,72,121,163,170,174],"time-dependent":[2],"drug":[3,176],"and":[4,40,61,77,80,84,87,107,118,133,193],"environmental":[5],"effects":[6,173],"on":[7,204],"single,":[8],"live":[9,100],"bacterial":[10,198],"cells":[11],"would":[12,31],"benefit":[13],"significantly":[14],"from":[15],"a":[16,62],"permeable,":[17],"nonperturbative,":[18],"long-lived":[19],"fluorescent":[20],"stain":[21,30],"specific":[22],"to":[23,147,186,196],"the":[24,70,92,104,119,129,134,150,164,171,175,180,194],"nucleoids":[25],"(chromosomal":[26],"DNA).":[27],"The":[28],"ideal":[29,97],"not":[32],"affect":[33],"cell":[34,116],"growth":[35],"rate":[36],"or":[37],"nucleoid":[38,110],"morphology":[39],"dynamics,":[41],"even":[42],"during":[43],"laser":[44,59],"illumination":[45],"for":[46,98,127],"hundreds":[47],"camera":[49,67],"frames.":[50],"In":[51,138,178],"this":[52],"study,":[53],"time-dependent,":[54],"single-cell":[55],"fluorescence":[56,145],"imaging":[57,99,146],"with":[58],"excitation":[60],"sensitive":[63],"electron-multiplying":[64],"charge-coupled-device":[65],"(EMCCD)":[66],"critically":[68],"tested":[69],"utility":[71],"\"dead-cell":[73],"stains\"":[74,82],"(SYTOX":[75],"orange":[76,124],"SYTOX":[78,123],"green)":[79],"\"live-cell":[81],"(DRAQ5":[83],"SYTO":[85],"61)":[86],"also":[88],"4',6-diamidino-2-phenylindole":[89],"(DAPI).":[90],"Surprisingly,":[91],"dead-cell":[93],"stains":[94,106],"were":[95],"nearly":[96],"Escherichia":[101],"coli,":[102],"while":[103,184],"live-cell":[105],"DAPI":[108],"caused":[109],"expansion":[111],"and,":[112],"in":[113,189],"some":[114],"cases,":[115],"permeabilization":[117,162],"halting":[120],"growth.":[122],"performed":[125],"well":[126],"both":[128],"Gram-negative":[130],"E.":[131,165],"coli":[132,166],"Gram-positive":[135],"Bacillus":[136],"subtilis.":[137],"an":[139],"initial":[140],"application,":[141],"we":[142],"used":[143],"two-color":[144],"show":[148],"that":[149],"antimicrobial":[151],"peptide":[152],"cecropin":[153,187],"A":[154,188],"destroyed":[155],"nucleoid-ribosome":[156,205],"segregation":[157],"over":[158],"20":[159],"min":[160],"after":[161],"cytoplasmic":[167],"membrane,":[168],"reminiscent":[169],"long-term":[172],"rifampin.":[177],"contrast,":[179],"human":[181],"cathelicidin":[182],"LL-37,":[183],"similar":[185],"structure,":[190],"length,":[191],"charge,":[192],"ability":[195],"permeabilize":[197],"membranes,":[199],"had":[200],"no":[201],"observable":[202],"effect":[203],"segregation.":[206],"Possible":[207],"underlying":[208],"causes":[209],"are":[210],"suggested.":[211]},"counts_by_year":[{"year":2026,"cited_by_count":4},{"year":2025,"cited_by_count":3},{"year":2024,"cited_by_count":3},{"year":2023,"cited_by_count":6},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":3},{"year":2020,"cited_by_count":6},{"year":2019,"cited_by_count":5},{"year":2018,"cited_by_count":7},{"year":2017,"cited_by_count":4},{"year":2016,"cited_by_count":5},{"year":2015,"cited_by_count":8},{"year":2014,"cited_by_count":2}],"updated_date":"2026-11-02T09:45:03.842371","created_date":"2016-06-24T00:00:00"}