{"id":"https://openalex.org/W2106904056","doi":"https://doi.org/10.1083/jcb.104.2.201","title":"Direct probing of the interaction between the signal sequence of nascent preprolactin and the signal recognition particle by specific cross-linking.","display_name":"Direct probing of the interaction between the signal sequence of nascent preprolactin and the signal recognition particle by specific cross-linking.","publication_year":1987,"publication_date":"1987-02-01","ids":{"openalex":"https://openalex.org/W2106904056","doi":"https://doi.org/10.1083/jcb.104.2.201","mag":"2106904056","pmid":"https://pubmed.ncbi.nlm.nih.gov/3643215","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/2114417"},"language":"en","primary_location":{"id":"doi:10.1083/jcb.104.2.201","doi":"https://doi.org/10.1083/jcb.104.2.201","is_oa":false,"landing_page_url":"https://doi.org/10.1083/jcb.104.2.201","pdf_url":null,"source":{"id":"https://openalex.org/S96410829","display_name":"The Journal of Cell Biology","issn_l":"0021-9525","issn":["0021-9525","1540-8140"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315676","host_organization_name":"Rockefeller University Press","host_organization_lineage":["https://openalex.org/P4310315676"],"host_organization_lineage_names":["Rockefeller University Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of cell biology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2114417","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5082217142","display_name":"Martin Wiedmann","orcid":"https://orcid.org/0000-0002-4168-5662","observed_orcids":["https://orcid.org/0000-0002-4168-5662"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"M Wiedmann","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5035824335","display_name":"Teymuras V. Kurzchalia","orcid":"https://orcid.org/0000-0002-1683-8460","observed_orcids":["https://orcid.org/0000-0002-1683-8460"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"T V Kurzchalia","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5058144463","display_name":"Heinz BIELKA","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"H Bielka","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5087757787","display_name":"Tom A. Rapoport","orcid":"https://orcid.org/0000-0001-9911-4216","observed_orcids":["https://orcid.org/0000-0001-9911-4216"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"T A Rapoport","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":0,"institutions_distinct_count":0,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":5.3561,"has_fulltext":false,"cited_by_count":116,"citation_normalized_percentile":{"value":0.96895922,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":96},"biblio":{"volume":"104","issue":"2","first_page":"201","last_page":"208"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.9218999743461609,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10521","display_name":"RNA and protein synthesis mechanisms","score":0.9218999743461609,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10617","display_name":"Cellular transport and secretion","score":0.046799998730421066,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11150","display_name":"Endoplasmic Reticulum Stress and Disease","score":0.015799999237060547,"subfield":{"id":"https://openalex.org/subfields/1307","display_name":"Cell Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/signal-recognition-particle","display_name":"signal recognition particle","score":0.9890000224113464},{"id":"https://openalex.org/keywords/signal-peptide","display_name":"signal peptide","score":0.7300000190734863},{"id":"https://openalex.org/keywords/protein-translocation","display_name":"protein translocation","score":0.7599999904632568},{"id":"https://openalex.org/keywords/endoplasmic-reticulum-membrane","display_name":"endoplasmic reticulum membrane","score":0.9409999847412109},{"id":"https://openalex.org/keywords/srp-receptor","display_name":"SRP receptor","score":0.7379999756813049},{"id":"https://openalex.org/keywords/nascent-polypeptide-chains","display_name":"nascent polypeptide chains","score":0.7820000052452087},{"id":"https://openalex.org/keywords/elongation-arrest","display_name":"elongation arrest","score":0.6779999732971191},{"id":"https://openalex.org/keywords/affinity-labeling","display_name":"affinity labeling","score":0.6729999780654907},{"id":"https://openalex.org/keywords/cross-linking","display_name":"crosslinking","score":0.6399999856948853},{"id":"https://openalex.org/keywords/ribosome","display_name":"ribosome","score":0.5770000219345093}],"concepts":[{"id":"https://openalex.org/C186175293","wikidata":"https://www.wikidata.org/wiki/Q424665","display_name":"Signal recognition particle","level":5,"score":0.9460206627845764},{"id":"https://openalex.org/C203900970","wikidata":"https://www.wikidata.org/wiki/Q21125262","display_name":"Signal recognition particle receptor","level":5,"score":0.8318597674369812},{"id":"https://openalex.org/C158617107","wikidata":"https://www.wikidata.org/wiki/Q79927","display_name":"Endoplasmic reticulum","level":2,"score":0.7925815582275391},{"id":"https://openalex.org/C10858879","wikidata":"https://www.wikidata.org/wiki/Q899790","display_name":"Signal peptide","level":4,"score":0.7551125288009644},{"id":"https://openalex.org/C88478588","wikidata":"https://www.wikidata.org/wiki/Q42244","display_name":"Ribosome","level":4,"score":0.7226027250289917},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.6520975828170776},{"id":"https://openalex.org/C199229279","wikidata":"https://www.wikidata.org/wiki/Q610647","display_name":"Translocon","level":4,"score":0.6519837379455566},{"id":"https://openalex.org/C559089031","wikidata":"https://www.wikidata.org/wiki/Q658882","display_name":"Protein targeting","level":4,"score":0.5985280871391296},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5196112990379333},{"id":"https://openalex.org/C12554922","wikidata":"https://www.wikidata.org/wiki/Q7100","display_name":"Biophysics","level":1,"score":0.5113827586174011},{"id":"https://openalex.org/C2779190341","wikidata":"https://www.wikidata.org/wiki/Q7512720","display_name":"Signal peptidase","level":5,"score":0.4293394088745117},{"id":"https://openalex.org/C167625842","wikidata":"https://www.wikidata.org/wiki/Q899763","display_name":"Peptide sequence","level":3,"score":0.41537678241729736},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4143241345882416},{"id":"https://openalex.org/C144647389","wikidata":"https://www.wikidata.org/wiki/Q423042","display_name":"Membrane protein","level":3,"score":0.32892632484436035},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.24916043877601624},{"id":"https://openalex.org/C67705224","wikidata":"https://www.wikidata.org/wiki/Q11053","display_name":"RNA","level":3,"score":0.14992177486419678},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001389","descriptor_name":"Azirines","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D001565","descriptor_name":"Benzoates","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D003432","descriptor_name":"Cross-Linking Reagents","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":true},{"descriptor_ui":"D003513","descriptor_name":"Cycloheximide","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D004285","descriptor_name":"Dogs","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D008970","descriptor_name":"Molecular Weight","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010179","descriptor_name":"Pancreas","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010944","descriptor_name":"Plants","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011388","descriptor_name":"Prolactin","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D011388","descriptor_name":"Prolactin","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D011498","descriptor_name":"Protein Precursors","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":true},{"descriptor_ui":"D011498","descriptor_name":"Protein Precursors","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012270","descriptor_name":"Ribosomes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D012346","descriptor_name":"RNA, Transfer, Amino Acyl","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D014176","descriptor_name":"Protein Biosynthesis","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014908","descriptor_name":"Triticum","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D021382","descriptor_name":"Protein Sorting Signals","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true}],"locations_count":5,"locations":[{"id":"doi:10.1083/jcb.104.2.201","doi":"https://doi.org/10.1083/jcb.104.2.201","is_oa":false,"landing_page_url":"https://doi.org/10.1083/jcb.104.2.201","pdf_url":null,"source":{"id":"https://openalex.org/S96410829","display_name":"The Journal of Cell Biology","issn_l":"0021-9525","issn":["0021-9525","1540-8140"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["anvur-scientific","cwts-core","dongbi-a","doyens","jufo-3","ki-jl-2","medline","norway-2","poland-140","russia-white-list-1"],"host_organization":"https://openalex.org/P4310315676","host_organization_name":"Rockefeller University Press","host_organization_lineage":["https://openalex.org/P4310315676"],"host_organization_lineage_names":["Rockefeller University Press"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of cell biology","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:915120","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2114417","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:2114417","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2114417","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Cell Biol","raw_type":"Text"},{"id":"pmid:3643215","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/3643215","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"The Journal of cell biology","raw_type":"Journal Article"},{"id":"pmh:oai:CiteSeerX.psu:10.1.1.277.7701","doi":null,"is_oa":false,"landing_page_url":"http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.277.7701","pdf_url":null,"source":null,"license":null,"license_id":null,"version":"submittedVersion","is_accepted":false,"is_published":false,"raw_source_name":"ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/8a/f5/J_Cell_Biol_1987_Feb_1_104(2)_201-208.tar.gz","raw_type":"text"}],"best_oa_location":{"id":"pmh:oai:europepmc.org:915120","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/2114417","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":"cc-by-nc-sa","license_id":"https://openalex.org/licenses/cc-by-nc-sa","version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":null,"raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":27,"referenced_works":["https://openalex.org/W124157494","https://openalex.org/W1123576991","https://openalex.org/W1584935191","https://openalex.org/W1957453673","https://openalex.org/W1968457431","https://openalex.org/W1973993781","https://openalex.org/W1976880811","https://openalex.org/W1988877989","https://openalex.org/W1997243880","https://openalex.org/W2018289835","https://openalex.org/W2024680024","https://openalex.org/W2073130442","https://openalex.org/W2076708118","https://openalex.org/W2079114119","https://openalex.org/W2090030959","https://openalex.org/W2098310715","https://openalex.org/W2100837269","https://openalex.org/W2108708484","https://openalex.org/W2120333646","https://openalex.org/W2128207929","https://openalex.org/W2131756959","https://openalex.org/W2137748564","https://openalex.org/W2145045711","https://openalex.org/W2164939857","https://openalex.org/W2169863303","https://openalex.org/W2171903383","https://openalex.org/W2215100787"],"related_works":["https://openalex.org/W2058273091","https://openalex.org/W2079114119","https://openalex.org/W7097631006","https://openalex.org/W2020558560","https://openalex.org/W2128207929","https://openalex.org/W2243212101","https://openalex.org/W7100872358","https://openalex.org/W2031290472","https://openalex.org/W2017648725","https://openalex.org/W2411672812"],"abstract_inverted_index":{"We":[0,138],"have":[1],"studied":[2],"the":[3,6,13,19,26,42,65,81,113,126,145,149,153,159,164],"interaction":[4],"between":[5],"signal":[7,14,82,127,165],"sequence":[8,128,166],"of":[9,33,59,84,104,107,148,158],"nascent":[10,85,114],"preprolactin":[11,86],"and":[12,62,92,111],"recognition":[15],"particle":[16],"(SRP)":[17],"during":[18],"initial":[20],"events":[21],"in":[22,48],"protein":[23,56],"translocation":[24],"across":[25],"endoplasmic":[27,160],"reticulum":[28,161],"membrane.":[29],"A":[30],"new":[31],"method":[32],"affinity":[34,95],"labeling":[35],"was":[36],"used,":[37],"whereby":[38],"lysine":[39],"residues,":[40,91],"carrying":[41],"photoreactive":[43],"group":[44],"4-(3-trifluoromethyldiazirino)":[45],"benzoic":[46],"acid":[47],"their":[49],"side":[50],"chains,":[51],"are":[52],"incorporated":[53],"into":[54],"a":[55,120],"by":[57,70],"means":[58],"modified":[60],"lysyl-tRNA,":[61],"cross-linking":[63],"to":[64,116,119,125],"interacting":[66],"component":[67,79],"is":[68,109,173],"induced":[69],"irradiation.":[71],"SRP":[72,108,123,141,156,168,172],"interacts":[73],"through":[74],"its":[75],"Mr":[76],"54,000":[77],"polypeptide":[78,150],"with":[80,93,96],"sequences":[83],"chains":[87,98],"containing":[88],"about":[89],"70":[90],"decreasing":[94],"longer":[97],"as":[99],"well;":[100],"it":[101,136],"causes":[102],"inhibition":[103],"elongation.":[105],"Binding":[106],"reversible":[110],"requires":[112],"chain":[115,151],"be":[117],"bound":[118],"functional":[121],"ribosome.":[122,154],"cross-linked":[124],"still":[129],"inhibits":[130],"elongation":[131,176],"but":[132],"does":[133,142],"not":[134,143],"prevent":[135],"completely.":[137],"conclude":[139],"that":[140],"block":[144],"exit":[146],"site":[147],"on":[152],"The":[155],"receptor":[157],"membrane":[162],"displaces":[163],"from":[167],"and,":[169],"even":[170],"if":[171],"cross-linked,":[174],"releases":[175],"arrest.":[177]},"counts_by_year":[{"year":2025,"cited_by_count":1},{"year":2020,"cited_by_count":1},{"year":2019,"cited_by_count":2},{"year":2017,"cited_by_count":1},{"year":2015,"cited_by_count":1}],"updated_date":"2026-11-04T10:54:05.112534","created_date":"2016-06-24T00:00:00"}