{"id":"https://openalex.org/W2106488041","doi":null,"title":"Surface expression and functional competence of CD3-independent TCR zeta-chains in immature thymocytes.","display_name":"Surface expression and functional competence of CD3-independent TCR zeta-chains in immature thymocytes.","publication_year":1999,"publication_date":"1999-03-01","ids":{"openalex":"https://openalex.org/W2106488041","mag":"2106488041","pmid":"https://pubmed.ncbi.nlm.nih.gov/10072500"},"language":"en","primary_location":{"id":"pmid:10072500","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10072500","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of immunology (Baltimore, Md. : 1950)","raw_type":"Journal Article"},"volume":null,"type":"article","indexed_in":["pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5064565562","display_name":"Fabio Grassi","orcid":"https://orcid.org/0000-0003-3348-1712","observed_orcids":["https://orcid.org/0000-0003-3348-1712"]},"institutions":[{"id":"https://openalex.org/I189158943","display_name":"University of Milan","ror":"https://ror.org/00wjc7c48","country_code":"IT","type":"education","lineage":["https://openalex.org/I189158943"]},{"id":"https://openalex.org/I4210133214","display_name":"IRCCS Ospedale San Raffaele","ror":"https://ror.org/039zxt351","country_code":"IT","type":"healthcare","lineage":["https://openalex.org/I4210133214","https://openalex.org/I4210153126"]}],"countries":["IT"],"is_corresponding":false,"raw_author_name":"F Grassi","raw_affiliation_strings":["Dipartimento di Biologia e Genetica per le Scienze Mediche, Universit\u00e0 di Milano at Department of Biological and Technological Research, San Raffaele Scientific Institute (HSR), Milan, Italy. fabio.grassi@hsr.it"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Dipartimento di Biologia e Genetica per le Scienze Mediche, Universit\u00e0 di Milano at Department of Biological and Technological Research, San Raffaele Scientific Institute (HSR), Milan, Italy. fabio.grassi@hsr.it","institution_ids":["https://openalex.org/I189158943","https://openalex.org/I4210133214"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5062494397","display_name":"Eliane Barbier","orcid":null,"observed_orcids":[]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"E Barbier","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5075625390","display_name":"Simona Porcellini","orcid":"https://orcid.org/0009-0002-6179-6287","observed_orcids":["https://orcid.org/0009-0002-6179-6287"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"S Porcellini","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"middle","author":{"id":"https://openalex.org/A5012087369","display_name":"Harald von Boehmer","orcid":"https://orcid.org/0000-0002-9714-1613","observed_orcids":["https://orcid.org/0000-0002-9714-1613"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"H von Boehmer","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]},{"author_position":"last","author":{"id":"https://openalex.org/A5077352590","display_name":"Pierre\u2010Andr\u00e9 Cazenave","orcid":"https://orcid.org/0000-0002-1345-8526","observed_orcids":["https://orcid.org/0000-0002-1345-8526"]},"institutions":[],"countries":[],"is_corresponding":false,"raw_author_name":"P A Cazenave","raw_affiliation_strings":[],"raw_orcid":null,"affiliations":[]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":2,"corresponding_author_ids":[],"corresponding_institution_ids":[],"apc_list":null,"apc_paid":null,"fwci":1.0264,"has_fulltext":false,"cited_by_count":15,"citation_normalized_percentile":{"value":0.75176275,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"162","issue":"5","first_page":"2589","last_page":"96"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10031","display_name":"T-cell and B-cell Immunology","score":0.9970999956130981,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T10031","display_name":"T-cell and B-cell Immunology","score":0.9970999956130981,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10469","display_name":"Psoriasis: Treatment and Pathogenesis","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2403","display_name":"Immunology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11204","display_name":"Ion Channels and Receptors","score":0.00019999999494757503,"subfield":{"id":"https://openalex.org/subfields/2809","display_name":"Sensory Systems"},"field":{"id":"https://openalex.org/fields/28","display_name":"Neuroscience"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/t-cell-receptor-zeta-chain","display_name":"T cell receptor zeta chain","score":0.8320000171661377},{"id":"https://openalex.org/keywords/immature-thymocytes","display_name":"immature thymocytes","score":0.8220000267028809},{"id":"https://openalex.org/keywords/double-negative-thymocytes","display_name":"double-negative thymocytes","score":0.8650000095367432},{"id":"https://openalex.org/keywords/zap-70","display_name":"ZAP-70","score":0.7889999747276306},{"id":"https://openalex.org/keywords/pre-tcr-signaling","display_name":"pre-TCR signaling","score":0.9490000009536743},{"id":"https://openalex.org/keywords/calnexin","display_name":"calnexin","score":0.7549999952316284},{"id":"https://openalex.org/keywords/t-cell-development","display_name":"T cell development","score":0.5109999775886536}],"concepts":[{"id":"https://openalex.org/C114684123","wikidata":"https://www.wikidata.org/wiki/Q419458","display_name":"CD3","level":4,"score":0.8034687042236328},{"id":"https://openalex.org/C2778954852","wikidata":"https://www.wikidata.org/wiki/Q7799635","display_name":"Thymocyte","level":4,"score":0.7747828364372253},{"id":"https://openalex.org/C19317047","wikidata":"https://www.wikidata.org/wiki/Q412037","display_name":"T-cell receptor","level":4,"score":0.6669273972511292},{"id":"https://openalex.org/C95444343","wikidata":"https://www.wikidata.org/wiki/Q7141","display_name":"Cell biology","level":1,"score":0.4991583824157715},{"id":"https://openalex.org/C153911025","wikidata":"https://www.wikidata.org/wiki/Q7202","display_name":"Molecular biology","level":1,"score":0.4852275848388672},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.46528783440589905},{"id":"https://openalex.org/C11960822","wikidata":"https://www.wikidata.org/wiki/Q242736","display_name":"Phosphorylation","level":2,"score":0.44705548882484436},{"id":"https://openalex.org/C130361206","wikidata":"https://www.wikidata.org/wiki/Q908443","display_name":"Calnexin","level":4,"score":0.41054025292396545},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.38073378801345825},{"id":"https://openalex.org/C2776090121","wikidata":"https://www.wikidata.org/wiki/Q193529","display_name":"T cell","level":3,"score":0.37924593687057495},{"id":"https://openalex.org/C167672396","wikidata":"https://www.wikidata.org/wiki/Q417800","display_name":"CD8","level":3,"score":0.3747028708457947},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.28253650665283203},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.1484871506690979},{"id":"https://openalex.org/C8891405","wikidata":"https://www.wikidata.org/wiki/Q1059","display_name":"Immune system","level":2,"score":0.10107335448265076},{"id":"https://openalex.org/C158617107","wikidata":"https://www.wikidata.org/wiki/Q79927","display_name":"Endoplasmic reticulum","level":2,"score":0.06434860825538635},{"id":"https://openalex.org/C47450691","wikidata":"https://www.wikidata.org/wiki/Q634510","display_name":"Calreticulin","level":3,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D002135","descriptor_name":"Calcium-Binding Proteins","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":false},{"descriptor_ui":"D002460","descriptor_name":"Cell Line","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004268","descriptor_name":"DNA-Binding Proteins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D008565","descriptor_name":"Membrane Proteins","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D008565","descriptor_name":"Membrane Proteins","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D008810","descriptor_name":"Mice, Inbred C57BL","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D010766","descriptor_name":"Phosphorylation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D011505","descriptor_name":"Protein-Tyrosine Kinases","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D011948","descriptor_name":"Receptors, Antigen, T-Cell","qualifier_ui":"Q000032","qualifier_name":"analysis","is_major_topic":false},{"descriptor_ui":"D011948","descriptor_name":"Receptors, Antigen, T-Cell","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D013601","descriptor_name":"T-Lymphocytes","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D016513","descriptor_name":"Mice, SCID","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D017252","descriptor_name":"CD3 Complex","qualifier_ui":"Q000502","qualifier_name":"physiology","is_major_topic":true},{"descriptor_ui":"D037281","descriptor_name":"Calnexin","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051379","descriptor_name":"Mice","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D051746","descriptor_name":"ZAP-70 Protein-Tyrosine Kinase","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"pmid:10072500","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10072500","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of immunology (Baltimore, Md. : 1950)","raw_type":"Journal Article"},{"id":"mag:2106488041","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/10072500","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":null,"is_accepted":false,"is_published":false,"raw_source_name":"PubMed","raw_type":null}],"best_oa_location":null,"sustainable_development_goals":[{"display_name":"Good health and well-being","score":0.6240000128746033,"id":"https://metadata.un.org/sdg/3"}],"sustainable_development_goals_aurora":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":78,"referenced_works":["https://openalex.org/W813816","https://openalex.org/W81274061","https://openalex.org/W129375328","https://openalex.org/W314850542","https://openalex.org/W1479717491","https://openalex.org/W1510108058","https://openalex.org/W1535548497","https://openalex.org/W1540334891","https://openalex.org/W1545434216","https://openalex.org/W1546074605","https://openalex.org/W1587792888","https://openalex.org/W1588885776","https://openalex.org/W1775928186","https://openalex.org/W1844994454","https://openalex.org/W1936444303","https://openalex.org/W1973992050","https://openalex.org/W1977546470","https://openalex.org/W1978903916","https://openalex.org/W1983960385","https://openalex.org/W1986543124","https://openalex.org/W1991102055","https://openalex.org/W1994947913","https://openalex.org/W1996441373","https://openalex.org/W1999298531","https://openalex.org/W2005150833","https://openalex.org/W2009335336","https://openalex.org/W2011389309","https://openalex.org/W2015778367","https://openalex.org/W2019174846","https://openalex.org/W2021680927","https://openalex.org/W2022284744","https://openalex.org/W2023688429","https://openalex.org/W2026337349","https://openalex.org/W2027497451","https://openalex.org/W2031202849","https://openalex.org/W2032046420","https://openalex.org/W2035223596","https://openalex.org/W2035820068","https://openalex.org/W2040134256","https://openalex.org/W2042173550","https://openalex.org/W2044219128","https://openalex.org/W2045941767","https://openalex.org/W2046901206","https://openalex.org/W2051283527","https://openalex.org/W2055446110","https://openalex.org/W2059257370","https://openalex.org/W2062999278","https://openalex.org/W2070379407","https://openalex.org/W2071081026","https://openalex.org/W2071632799","https://openalex.org/W2072984471","https://openalex.org/W2073366334","https://openalex.org/W2074249987","https://openalex.org/W2079749432","https://openalex.org/W2083453910","https://openalex.org/W2084627584","https://openalex.org/W2087025778","https://openalex.org/W2090495447","https://openalex.org/W2095213694","https://openalex.org/W2103129136","https://openalex.org/W2109606742","https://openalex.org/W2112846984","https://openalex.org/W2115420598","https://openalex.org/W2121655252","https://openalex.org/W2126464081","https://openalex.org/W2127324955","https://openalex.org/W2130147879","https://openalex.org/W2131894799","https://openalex.org/W2134973492","https://openalex.org/W2135503300","https://openalex.org/W2142463988","https://openalex.org/W2144935474","https://openalex.org/W2145568913","https://openalex.org/W2147149166","https://openalex.org/W2151206902","https://openalex.org/W2170891925","https://openalex.org/W2181083086","https://openalex.org/W2188994821"],"related_works":["https://openalex.org/W4313347963","https://openalex.org/W2057501114","https://openalex.org/W1911451086","https://openalex.org/W2109606742","https://openalex.org/W1772549878","https://openalex.org/W2112835801","https://openalex.org/W1974788802","https://openalex.org/W2045941767","https://openalex.org/W1562464953","https://openalex.org/W2082236028"],"abstract_inverted_index":{"In":[0,30],"recombinase-deficient":[1],"(RAG-2-/-)":[2],"mice,":[3],"double-negative":[4],"thymocytes":[5,78],"can":[6,37],"be":[7,38],"stimulated":[8],"to":[9,108],"proliferate":[10],"and":[11,79,124],"differentiate":[12],"by":[13,115],"anti-CD3":[14],"Abs.":[15],"CD3":[16,74],"molecules":[17],"are":[18,55],"expressed":[19],"on":[20],"the":[21,60,73,89,98,116],"surface":[22],"of":[23,65,69,92],"these":[24,103],"cells":[25],"in":[26,43,76,81,97,119],"association":[27,44,68,91],"with":[28,45,59,72,95],"calnexin.":[29],"this":[31],"study,":[32],"we":[33],"show":[34],"that":[35,102,118],"zeta-chains":[36],"recovered":[39],"as":[40,86,88],"phosphorylated":[41,46],"proteins":[42],"ZAP-70":[47,96,123],"from":[48],"anti-CD3-stimulated":[49],"RAG-2-/-":[50,77,99,120],"thymocytes,":[51,100,122],"even":[52],"though":[53],"they":[54],"not":[56],"demonstrably":[57],"associated":[58],"CD3/calnexin":[61],"complex.":[62],"The":[63],"lack":[64],"a":[66,82],"physical":[67],"zeta":[70,93],"dimers":[71,94,105],"complex":[75],"also":[80],"pre-TCR-expressing":[83],"cell":[84],"line,":[85],"well":[87],"efficient":[90],"suggest":[101],"zeta-chain":[104],"could":[106],"contribute":[107],"pre-TCR":[109],"signaling.":[110],"This":[111],"idea":[112],"is":[113],"supported":[114],"finding":[117],"zeta-deficient":[121],"p120cbl":[125],"were":[126],"only":[127],"weakly":[128],"phosphorylated.":[129]},"counts_by_year":[{"year":2013,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}