{"id":"https://openalex.org/W2105774302","doi":"https://doi.org/10.1002/prot.340220108","title":"Purification and crystallization of a schistosomal glutathione S\u2010Transferase","display_name":"Purification and crystallization of a schistosomal glutathione S\u2010Transferase","publication_year":1995,"publication_date":"1995-05-01","ids":{"openalex":"https://openalex.org/W2105774302","doi":"https://doi.org/10.1002/prot.340220108","mag":"2105774302","pmid":"https://pubmed.ncbi.nlm.nih.gov/7675787"},"language":"en","primary_location":{"id":"doi:10.1002/prot.340220108","doi":"https://doi.org/10.1002/prot.340220108","is_oa":false,"landing_page_url":"https://doi.org/10.1002/prot.340220108","pdf_url":null,"source":{"id":"https://openalex.org/S121161810","display_name":"Proteins Structure Function and Bioinformatics","issn_l":"0887-3585","issn":["0887-3585","1097-0134"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proteins: Structure, Function, and Bioinformatics","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5031081898","display_name":"Michele A. McTigue","orcid":"https://orcid.org/0000-0002-4877-4414","observed_orcids":["https://orcid.org/0000-0002-4877-4414"]},"institutions":[{"id":"https://openalex.org/I123431417","display_name":"Scripps Research Institute","ror":"https://ror.org/02dxx6824","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I123431417"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Michele A. McTigue","raw_affiliation_strings":["Department of Molecular Biology, Scripps Research Institute, La Jolla, California 92037, USA","Scripps Research Institute#TAB#"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Biology, Scripps Research Institute, La Jolla, California 92037, USA","institution_ids":["https://openalex.org/I123431417"]},{"raw_affiliation_string":"Scripps Research Institute#TAB#","institution_ids":["https://openalex.org/I123431417"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5004970681","display_name":"Susan L. Bernstein","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I123431417","display_name":"Scripps Research Institute","ror":"https://ror.org/02dxx6824","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I123431417"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"Susan L. Bernstein","raw_affiliation_strings":["Department of Molecular Biology, The Scripps Research Institute, La Jolla, California, 92037","Scripps Research Institute#TAB#"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Biology, The Scripps Research Institute, La Jolla, California, 92037","institution_ids":["https://openalex.org/I123431417"]},{"raw_affiliation_string":"Scripps Research Institute#TAB#","institution_ids":["https://openalex.org/I123431417"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5068870718","display_name":"DeWight R. Williams","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I123431417","display_name":"Scripps Research Institute","ror":"https://ror.org/02dxx6824","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I123431417"]}],"countries":["US"],"is_corresponding":false,"raw_author_name":"DeWight R. Williams","raw_affiliation_strings":["Department of Molecular Biology, The Scripps Research Institute, La Jolla, California, 92037","Scripps Research Institute#TAB#"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Biology, The Scripps Research Institute, La Jolla, California, 92037","institution_ids":["https://openalex.org/I123431417"]},{"raw_affiliation_string":"Scripps Research Institute#TAB#","institution_ids":["https://openalex.org/I123431417"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5059713279","display_name":"John A. Tainer","orcid":"https://orcid.org/0000-0003-1659-2429","observed_orcids":["https://orcid.org/0000-0003-1659-2429"]},"institutions":[{"id":"https://openalex.org/I123431417","display_name":"Scripps Research Institute","ror":"https://ror.org/02dxx6824","country_code":"US","type":"nonprofit","lineage":["https://openalex.org/I123431417"]}],"countries":["US"],"is_corresponding":true,"raw_author_name":"John A. Tainer","raw_affiliation_strings":["Department of Molecular Biology, The Scripps Research Institute, La Jolla, California, 92037","Molecular & Cellular Oncology"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Department of Molecular Biology, The Scripps Research Institute, La Jolla, California, 92037","institution_ids":["https://openalex.org/I123431417"]},{"raw_affiliation_string":"Molecular & Cellular Oncology","institution_ids":[]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5059713279"],"corresponding_institution_ids":["https://openalex.org/I123431417"],"apc_list":{"value":4550,"currency":"USD","value_usd":4550},"apc_paid":null,"fwci":0.9591,"has_fulltext":false,"cited_by_count":17,"citation_normalized_percentile":{"value":0.73853484,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":89,"max":94},"biblio":{"volume":"22","issue":"1","first_page":"55","last_page":"57"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.8999000191688538,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11162","display_name":"Enzyme Structure and Function","score":0.8999000191688538,"subfield":{"id":"https://openalex.org/subfields/1315","display_name":"Structural Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T12187","display_name":"Glutathione Transferases and Polymorphisms","score":0.08780000358819962,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10695","display_name":"Parasites and Host Interactions","score":0.005900000222027302,"subfield":{"id":"https://openalex.org/subfields/2405","display_name":"Parasitology"},"field":{"id":"https://openalex.org/fields/24","display_name":"Immunology and Microbiology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/glutathione-s-transferase","display_name":"glutathione S-transferase","score":0.9419999718666077},{"id":"https://openalex.org/keywords/recombinant-protein-purification","display_name":"recombinant protein purification","score":0.7960000038146973},{"id":"https://openalex.org/keywords/protein-crystallization","display_name":"protein crystallization","score":0.9480000138282776},{"id":"https://openalex.org/keywords/ammonium-sulfate-precipitant","display_name":"ammonium sulfate precipitant","score":0.703000009059906}],"concepts":[{"id":"https://openalex.org/C2780925461","wikidata":"https://www.wikidata.org/wiki/Q191831","display_name":"Ammonium sulfate","level":2,"score":0.8016058206558228},{"id":"https://openalex.org/C40767141","wikidata":"https://www.wikidata.org/wiki/Q285697","display_name":"Recombinant DNA","level":3,"score":0.6845000982284546},{"id":"https://openalex.org/C203036418","wikidata":"https://www.wikidata.org/wiki/Q284256","display_name":"Crystallization","level":2,"score":0.6649488210678101},{"id":"https://openalex.org/C166940927","wikidata":"https://www.wikidata.org/wiki/Q178827","display_name":"Monomer","level":3,"score":0.6035227179527283},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5643847584724426},{"id":"https://openalex.org/C138268822","wikidata":"https://www.wikidata.org/wiki/Q1051925","display_name":"Resolution (logic)","level":2,"score":0.5196846127510071},{"id":"https://openalex.org/C2776376580","wikidata":"https://www.wikidata.org/wiki/Q407355","display_name":"Transferase","level":3,"score":0.4994664192199707},{"id":"https://openalex.org/C538909803","wikidata":"https://www.wikidata.org/wiki/Q116907","display_name":"Glutathione","level":3,"score":0.49889397621154785},{"id":"https://openalex.org/C2776907368","wikidata":"https://www.wikidata.org/wiki/Q307302","display_name":"Glutathione S-transferase","level":4,"score":0.4641585946083069},{"id":"https://openalex.org/C147483822","wikidata":"https://www.wikidata.org/wiki/Q103537","display_name":"Antigen","level":2,"score":0.45972201228141785},{"id":"https://openalex.org/C3019047270","wikidata":"https://www.wikidata.org/wiki/Q307302","display_name":"Glutathione transferase","level":4,"score":0.4167289733886719},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.3934205174446106},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.34881019592285156},{"id":"https://openalex.org/C8010536","wikidata":"https://www.wikidata.org/wiki/Q160398","display_name":"Crystallography","level":1,"score":0.34826648235321045},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.24714395403862},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.24223682284355164},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.08566614985466003},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.08016276359558105},{"id":"https://openalex.org/C203014093","wikidata":"https://www.wikidata.org/wiki/Q101929","display_name":"Immunology","level":1,"score":0.07858070731163025},{"id":"https://openalex.org/C521977710","wikidata":"https://www.wikidata.org/wiki/Q81163","display_name":"Polymer","level":2,"score":0.07441431283950806},{"id":"https://openalex.org/C154945302","wikidata":"https://www.wikidata.org/wiki/Q11660","display_name":"Artificial intelligence","level":1,"score":0.0},{"id":"https://openalex.org/C41008148","wikidata":"https://www.wikidata.org/wiki/Q21198","display_name":"Computer science","level":0,"score":0.0}],"mesh":[{"descriptor_ui":"D000818","descriptor_name":"Animals","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000947","descriptor_name":"Antigens, Helminth","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D000947","descriptor_name":"Antigens, Helminth","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D000947","descriptor_name":"Antigens, Helminth","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D003460","descriptor_name":"Crystallization","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D004926","descriptor_name":"Escherichia coli","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D005982","descriptor_name":"Glutathione Transferase","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D005982","descriptor_name":"Glutathione Transferase","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D005982","descriptor_name":"Glutathione Transferase","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":true},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":false},{"descriptor_ui":"D011994","descriptor_name":"Recombinant Proteins","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D012547","descriptor_name":"Schistosoma","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D012547","descriptor_name":"Schistosoma","qualifier_ui":"Q000235","qualifier_name":"genetics","is_major_topic":false},{"descriptor_ui":"D018360","descriptor_name":"Crystallography, X-Ray","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1002/prot.340220108","doi":"https://doi.org/10.1002/prot.340220108","is_oa":false,"landing_page_url":"https://doi.org/10.1002/prot.340220108","pdf_url":null,"source":{"id":"https://openalex.org/S121161810","display_name":"Proteins Structure Function and Bioinformatics","issn_l":"0887-3585","issn":["0887-3585","1097-0134"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-c","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320595","host_organization_name":"Wiley","host_organization_lineage":["https://openalex.org/P4310320595"],"host_organization_lineage_names":["Wiley"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proteins: Structure, Function, and Bioinformatics","raw_type":"journal-article"},{"id":"pmid:7675787","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/7675787","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Proteins","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[{"id":"https://openalex.org/G191269165","display_name":"N GONORRHOEAE FERRIC BINDING PROTEIN STRUCTURE","funder_award_id":"F32AI008803","funder_id":"https://openalex.org/F4320337355","funder_display_name":"National Institute of Allergy and Infectious Diseases"},{"id":"https://openalex.org/G6442570698","display_name":"Superoxide Dismutase Structures and Lou Gehrigs Disease","funder_award_id":"R01GM039345","funder_id":"https://openalex.org/F4320337354","funder_display_name":"National Institute of General Medical Sciences"}],"funders":[{"id":"https://openalex.org/F4320332161","display_name":"National Institutes of Health","ror":"https://ror.org/01cwqze88"},{"id":"https://openalex.org/F4320337354","display_name":"National Institute of General Medical Sciences","ror":"https://ror.org/04q48ey07"},{"id":"https://openalex.org/F4320337355","display_name":"National Institute of Allergy and Infectious Diseases","ror":"https://ror.org/043z4tv69"}],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":11,"referenced_works":["https://openalex.org/W337892304","https://openalex.org/W1489526459","https://openalex.org/W2014694459","https://openalex.org/W2017594050","https://openalex.org/W2022708000","https://openalex.org/W2040758860","https://openalex.org/W2098539405","https://openalex.org/W2144860058","https://openalex.org/W2154011403","https://openalex.org/W4210991909","https://openalex.org/W4250682311"],"related_works":["https://openalex.org/W1978665392","https://openalex.org/W1991466537","https://openalex.org/W2055468432","https://openalex.org/W2075356247","https://openalex.org/W2059499224","https://openalex.org/W2356524603","https://openalex.org/W1974831205","https://openalex.org/W2016050407","https://openalex.org/W2362660221","https://openalex.org/W4388686904"],"abstract_inverted_index":{"The":[0,39,77],"26-kDa":[1],"glutathione":[2,30],"S-transferase":[3],"from":[4],"Schistosoma":[5],"japonica":[6],"(Sj26),":[7],"a":[8,29,90],"potential":[9],"antischistosomal":[10],"vaccine":[11],"antigen,":[12],"has":[13,115],"been":[14,116],"crystallized":[15],"in":[16,24],"an":[17,49],"unligated":[18,37],"form.":[19],"Sj26":[20,47,60,104],"was":[21],"recombinantly":[22],"produced":[23],"E.":[25],"coli":[26],"without":[27],"using":[28,68],"affinity":[31],"column":[32],"to":[33,54,80,118],"facilitate":[34],"preparation":[35],"of":[36,46,58],"enzyme.":[38],"recombinant":[40,59],"protein":[41],"contains":[42],"all":[43],"218":[44],"residues":[45,52],"and":[48,96,101],"additional":[50],"13":[51],"linked":[53],"the":[55,64,72,81],"C-terminus.":[56],"Crystals":[57],"were":[61],"obtained":[62,117],"by":[63],"vapor":[65],"diffusion":[66],"method":[67],"ammonium":[69],"sulfate":[70],"as":[71],"precipitant":[73],"at":[74],"pH":[75],"5.6.":[76],"crystals":[78],"belong":[79],"hexagonal":[82],"space":[83],"group":[84],"P6(3)22":[85],"with":[86],"unit":[87],"cell":[88],"dimensions":[89],"=":[91,93,98],"b":[92],"125.2":[94],"A":[95,100,109,120],"c":[97],"72.0":[99],"contain":[102],"one":[103],"monomer":[105],"per":[106],"asymmetric":[107],"unit.":[108],"complete":[110],"native":[111],"diffraction":[112],"data":[113],"set":[114],"2.4":[119],"resolution.":[121]},"counts_by_year":[{"year":2019,"cited_by_count":1},{"year":2012,"cited_by_count":1}],"updated_date":"2026-11-06T12:05:46.655746","created_date":"2016-06-24T00:00:00"}