{"id":"https://openalex.org/W2105598620","doi":"https://doi.org/10.1128/jb.172.6.3066-3072.1990","title":"Carboxyphosphonoenolpyruvate phosphonomutase, a novel enzyme catalyzing C-P bond formation","display_name":"Carboxyphosphonoenolpyruvate phosphonomutase, a novel enzyme catalyzing C-P bond formation","publication_year":1990,"publication_date":"1990-06-01","ids":{"openalex":"https://openalex.org/W2105598620","doi":"https://doi.org/10.1128/jb.172.6.3066-3072.1990","mag":"2105598620","pmid":"https://pubmed.ncbi.nlm.nih.gov/2160937","pmcid":"https://www.ncbi.nlm.nih.gov/pmc/articles/209109"},"language":"en","primary_location":{"id":"doi:10.1128/jb.172.6.3066-3072.1990","doi":"https://doi.org/10.1128/jb.172.6.3066-3072.1990","is_oa":false,"landing_page_url":"https://doi.org/10.1128/jb.172.6.3066-3072.1990","pdf_url":null,"source":{"id":"https://openalex.org/S133274750","display_name":"Journal of Bacteriology","issn_l":"0021-9193","issn":["0021-9193","1067-8832","1098-5530"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Bacteriology","raw_type":"journal-article"},"volume":null,"type":"article","indexed_in":["crossref","doaj","pubmed"],"open_access":{"is_oa":true,"oa_status":"green","oa_url":"http://europepmc.org/pmc/articles/PMC209109","any_repository_has_fulltext":true},"authorships":[{"author_position":"first","author":{"id":null,"display_name":"T Hidaka","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"T Hidaka","raw_affiliation_strings":["Institute of Applied Microbiology, University of Tokyo, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Applied Microbiology, University of Tokyo, Japan","institution_ids":["https://openalex.org/I74801974"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5016192973","display_name":"Satoshi Imai","orcid":"https://orcid.org/0000-0002-6251-4387","observed_orcids":["https://orcid.org/0000-0002-6251-4387"]},"institutions":[{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"S Imai","raw_affiliation_strings":["Institute of Applied Microbiology, University of Tokyo, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Applied Microbiology, University of Tokyo, Japan","institution_ids":["https://openalex.org/I74801974"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5037142201","display_name":"Osamu Hara","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"O Hara","raw_affiliation_strings":["Institute of Applied Microbiology, University of Tokyo, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Applied Microbiology, University of Tokyo, Japan","institution_ids":["https://openalex.org/I74801974"]}]},{"author_position":"middle","author":{"id":null,"display_name":"H Anzai","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"H Anzai","raw_affiliation_strings":["Institute of Applied Microbiology, University of Tokyo, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Applied Microbiology, University of Tokyo, Japan","institution_ids":["https://openalex.org/I74801974"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5101919013","display_name":"Takeshi Murakami","orcid":"https://orcid.org/0000-0003-0621-327X","observed_orcids":["https://orcid.org/0000-0003-0621-327X","https://orcid.org/0009-0000-4747-317X"]},"institutions":[{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"T Murakami","raw_affiliation_strings":["Institute of Applied Microbiology, University of Tokyo, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Applied Microbiology, University of Tokyo, Japan","institution_ids":["https://openalex.org/I74801974"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5113661180","display_name":"KATSUHIKO NAGAOKA","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"K Nagaoka","raw_affiliation_strings":["Institute of Applied Microbiology, University of Tokyo, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Applied Microbiology, University of Tokyo, Japan","institution_ids":["https://openalex.org/I74801974"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5111698724","display_name":"Haruo Seto","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I74801974","display_name":"The University of Tokyo","ror":"https://ror.org/057zh3y96","country_code":"JP","type":"education","lineage":["https://openalex.org/I74801974"]}],"countries":["JP"],"is_corresponding":false,"raw_author_name":"H Seto","raw_affiliation_strings":["Institute of Applied Microbiology, University of Tokyo, Japan"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Institute of Applied Microbiology, University of Tokyo, Japan","institution_ids":["https://openalex.org/I74801974"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":[],"corresponding_institution_ids":["https://openalex.org/I74801974"],"apc_list":null,"apc_paid":null,"fwci":4.1933,"has_fulltext":false,"cited_by_count":52,"citation_normalized_percentile":{"value":0.94570975,"is_in_top_1_percent":false,"is_in_top_10_percent":true},"cited_by_percentile_year":{"min":88,"max":98},"biblio":{"volume":"172","issue":"6","first_page":"3066","last_page":"3072"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T10252","display_name":"Microbial Natural Products and Biosynthesis","score":0.913100004196167,"subfield":{"id":"https://openalex.org/subfields/2736","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},"topics":[{"id":"https://openalex.org/T10252","display_name":"Microbial Natural Products and Biosynthesis","score":0.913100004196167,"subfield":{"id":"https://openalex.org/subfields/2736","display_name":"Pharmacology"},"field":{"id":"https://openalex.org/fields/27","display_name":"Medicine"},"domain":{"id":"https://openalex.org/domains/4","display_name":"Health Sciences"}},{"id":"https://openalex.org/T11748","display_name":"Organophosphorus compounds synthesis","score":0.0737999975681305,"subfield":{"id":"https://openalex.org/subfields/1605","display_name":"Organic Chemistry"},"field":{"id":"https://openalex.org/fields/16","display_name":"Chemistry"},"domain":{"id":"https://openalex.org/domains/3","display_name":"Physical Sciences"}},{"id":"https://openalex.org/T10120","display_name":"Bacterial Genetics and Biotechnology","score":0.00279999990016222,"subfield":{"id":"https://openalex.org/subfields/1311","display_name":"Genetics"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/c-p-bond-formation","display_name":"C\u2013P bond formation","score":0.9660000205039978},{"id":"https://openalex.org/keywords/streptomyces-hygroscopicus","display_name":"Streptomyces hygroscopicus","score":0.9800000190734863},{"id":"https://openalex.org/keywords/phosphoenolpyruvate","display_name":"phosphoenolpyruvate","score":0.8849999904632568},{"id":"https://openalex.org/keywords/biosynthetic-intermediates","display_name":"biosynthetic intermediates","score":0.7770000100135803},{"id":"https://openalex.org/keywords/antibiotic-biosynthesis","display_name":"antibiotic biosynthesis","score":0.6629999876022339},{"id":"https://openalex.org/keywords/enzyme-catalysis","display_name":"enzyme catalysis","score":0.4620000123977661}],"concepts":[{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.7844430804252625},{"id":"https://openalex.org/C2778023031","wikidata":"https://www.wikidata.org/wiki/Q3975996","display_name":"Streptomyces hygroscopicus","level":4,"score":0.6733382940292358},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.6304898262023926},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.6072999835014343},{"id":"https://openalex.org/C71240020","wikidata":"https://www.wikidata.org/wiki/Q186011","display_name":"Stereochemistry","level":1,"score":0.5544890761375427},{"id":"https://openalex.org/C143065580","wikidata":"https://www.wikidata.org/wiki/Q3285695","display_name":"Mutant","level":3,"score":0.5202856659889221},{"id":"https://openalex.org/C553450214","wikidata":"https://www.wikidata.org/wiki/Q851162","display_name":"Biosynthesis","level":3,"score":0.4860153794288635},{"id":"https://openalex.org/C512185932","wikidata":"https://www.wikidata.org/wiki/Q28745","display_name":"Nucleotide","level":3,"score":0.45180973410606384},{"id":"https://openalex.org/C515207424","wikidata":"https://www.wikidata.org/wiki/Q8066","display_name":"Amino acid","level":2,"score":0.43133246898651123},{"id":"https://openalex.org/C2779920670","wikidata":"https://www.wikidata.org/wiki/Q1144013","display_name":"Streptomyces","level":3,"score":0.39836394786834717},{"id":"https://openalex.org/C104317684","wikidata":"https://www.wikidata.org/wiki/Q7187","display_name":"Gene","level":2,"score":0.2425609529018402},{"id":"https://openalex.org/C523546767","wikidata":"https://www.wikidata.org/wiki/Q10876","display_name":"Bacteria","level":2,"score":0.210421621799469},{"id":"https://openalex.org/C54355233","wikidata":"https://www.wikidata.org/wiki/Q7162","display_name":"Genetics","level":1,"score":0.11708343029022217},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.09707257151603699}],"mesh":[{"descriptor_ui":"D000900","descriptor_name":"Anti-Bacterial Agents","qualifier_ui":"Q000096","qualifier_name":"biosynthesis","is_major_topic":true},{"descriptor_ui":"D004591","descriptor_name":"Electrophoresis, Polyacrylamide Gel","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009154","descriptor_name":"Mutation","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D009943","descriptor_name":"Organophosphorus Compounds","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010728","descriptor_name":"Phosphoenolpyruvate","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":false},{"descriptor_ui":"D010770","descriptor_name":"Phosphotransferases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D013302","descriptor_name":"Streptomyces","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D017875","descriptor_name":"Phosphotransferases (Phosphomutases)","qualifier_ui":null,"qualifier_name":null,"is_major_topic":true}],"locations_count":4,"locations":[{"id":"doi:10.1128/jb.172.6.3066-3072.1990","doi":"https://doi.org/10.1128/jb.172.6.3066-3072.1990","is_oa":false,"landing_page_url":"https://doi.org/10.1128/jb.172.6.3066-3072.1990","pdf_url":null,"source":{"id":"https://openalex.org/S133274750","display_name":"Journal of Bacteriology","issn_l":"0021-9193","issn":["0021-9193","1067-8832","1098-5530"],"is_oa":false,"is_in_doaj":true,"is_core":true,"listed_in":["cwts-core","doaj","dongbi-b","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-100","russia-white-list-2"],"host_organization":"https://openalex.org/P4310320263","host_organization_name":"American Society for Microbiology","host_organization_lineage":["https://openalex.org/P4310320263"],"host_organization_lineage_names":["American Society for Microbiology"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of Bacteriology","raw_type":"journal-article"},{"id":"pmh:oai:europepmc.org:69279","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC209109","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},{"id":"pmh:oai:pubmedcentral.nih.gov:209109","doi":null,"is_oa":true,"landing_page_url":"https://www.ncbi.nlm.nih.gov/pmc/articles/209109","pdf_url":null,"source":{"id":"https://openalex.org/S2764455111","display_name":"PubMed Central","issn_l":null,"issn":null,"is_oa":true,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"J Bacteriol","raw_type":"Text"},{"id":"pmid:2160937","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/2160937","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Journal of bacteriology","raw_type":"Journal Article"}],"best_oa_location":{"id":"pmh:oai:europepmc.org:69279","doi":null,"is_oa":true,"landing_page_url":"http://europepmc.org/pmc/articles/PMC209109","pdf_url":null,"source":{"id":"https://openalex.org/S4306400806","display_name":"Europe PMC (PubMed Central)","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1303153112","host_organization_name":"European Bioinformatics Institute","host_organization_lineage":["https://openalex.org/I1303153112"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"","raw_type":"Text"},"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"pdf":false,"grobid_xml":false},"content_urls":null,"referenced_works_count":18,"referenced_works":["https://openalex.org/W1986657244","https://openalex.org/W1997672726","https://openalex.org/W2013789049","https://openalex.org/W2018289835","https://openalex.org/W2021354078","https://openalex.org/W2027026862","https://openalex.org/W2037111948","https://openalex.org/W2041731378","https://openalex.org/W2054146826","https://openalex.org/W2058803779","https://openalex.org/W2060336559","https://openalex.org/W2070446713","https://openalex.org/W2075738852","https://openalex.org/W2081054712","https://openalex.org/W2100837269","https://openalex.org/W2136543379","https://openalex.org/W2326519151","https://openalex.org/W4237567863"],"related_works":["https://openalex.org/W2020418559","https://openalex.org/W2081368230","https://openalex.org/W1987267808","https://openalex.org/W2062877427","https://openalex.org/W2003130713","https://openalex.org/W2020153436","https://openalex.org/W2075738852","https://openalex.org/W2012725510","https://openalex.org/W1775557514","https://openalex.org/W1987155874"],"abstract_inverted_index":{"An":[0],"enzyme":[1],"catalyzing":[2],"the":[3,14,17,24,55,58,67,79,83,94,107,122,125,132],"formation":[4],"of":[5,16,27,31,54,66,70,82,93,99,110,117,124,128,135],"an":[6],"unusual":[7],"C-P":[8,133],"bond":[9,134],"that":[10,85],"is":[11],"involved":[12],"in":[13,47,52,57],"biosynthesis":[15],"antibiotic":[18],"bialaphos":[19],"(BA)":[20],"was":[21,40,103],"isolated":[22,105],"from":[23,106],"cell":[25],"extract":[26],"a":[28,44,48,96,113],"mutant":[29,49],"(NP71)":[30],"Streptomyces":[32],"hygroscopicus":[33],"SF1293.":[34],"This":[35],"enzyme,":[36,95],"carboxyphosphonoenolpyruvate":[37],"(CPEP)":[38],"phosphonomutase,":[39],"first":[41,63],"identified":[42],"as":[43,112],"protein":[45,72],"lacking":[46],"(NP213)":[50],"defective":[51],"one":[53],"steps":[56],"pathway":[59],"to":[60,75,89,130],"BA.":[61,118],"The":[62,91],"30":[64],"residues":[65],"amino":[68],"terminus":[69],"this":[71],"were":[73],"identical":[74],"those":[76],"predicted":[77],"by":[78],"nucleotide":[80],"sequence":[81],"gene":[84],"restored":[86],"BA":[87],"production":[88],"NP213.":[90],"substrate":[92],"P-carboxylated":[97],"derivative":[98],"phosphoenolpyruvate":[100],"named":[101],"CPEP,":[102],"also":[104],"broth":[108],"filtrate":[109],"NP213":[111],"new":[114],"biosynthetic":[115],"intermediate":[116],"CPEP":[119,129],"phosphonomutase":[120],"catalyzes":[121],"rearrangement":[123],"carboxyphosphono":[126],"group":[127],"form":[131],"phosphinopyruvate.":[136]},"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2025,"cited_by_count":2},{"year":2023,"cited_by_count":1},{"year":2021,"cited_by_count":2},{"year":2020,"cited_by_count":1},{"year":2016,"cited_by_count":3},{"year":2015,"cited_by_count":1},{"year":2013,"cited_by_count":5}],"updated_date":"2026-10-31T12:09:11.096373","created_date":"2016-06-24T00:00:00"}