{"id":"https://openalex.org/W2105552913","doi":"https://doi.org/10.1007/s12010-012-9940-0","title":"Purification and Biochemical Characterization of a Novel Alkaline (Phospho)lipase from a Newly Isolated Fusarium solani Strain","display_name":"Purification and Biochemical Characterization of a Novel Alkaline (Phospho)lipase from a Newly Isolated Fusarium solani Strain","publication_year":2012,"publication_date":"2012-11-14","ids":{"openalex":"https://openalex.org/W2105552913","doi":"https://doi.org/10.1007/s12010-012-9940-0","mag":"2105552913","pmid":"https://pubmed.ncbi.nlm.nih.gov/23151966"},"language":"en","primary_location":{"id":"doi:10.1007/s12010-012-9940-0","doi":"https://doi.org/10.1007/s12010-012-9940-0","is_oa":false,"landing_page_url":"https://doi.org/10.1007/s12010-012-9940-0","pdf_url":null,"source":{"id":"https://openalex.org/S153285774","display_name":"Applied Biochemistry and Biotechnology","issn_l":"0273-2289","issn":["0273-2289","1559-0291"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied Biochemistry and Biotechnology","raw_type":"journal-article"},"volume":null,"type":"article","is_traditional":true,"indexed_in":["crossref","pubmed"],"open_access":{"is_oa":false,"oa_status":"closed","oa_url":null,"any_repository_has_fulltext":false},"authorships":[{"author_position":"first","author":{"id":"https://openalex.org/A5060912367","display_name":"Raida Jallouli","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210111777","display_name":"Laboratoire de Biochimie","ror":"https://ror.org/01thw2g46","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I190752583","https://openalex.org/I2746051580","https://openalex.org/I4210111777","https://openalex.org/I4210139971","https://openalex.org/I4210161506","https://openalex.org/I98910050"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Raida Jallouli","raw_affiliation_strings":["Laboratoire de Biochimie et de G\u00e9nie Enzymatique des Lipases, ENIS route de Soukra, BPW 3038, Sfax, Tunisia","Laboratoire de Biochimie et de G\u00e9nie Enzymatique des Lipases"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratoire de Biochimie et de G\u00e9nie Enzymatique des Lipases, ENIS route de Soukra, BPW 3038, Sfax, Tunisia","institution_ids":[]},{"raw_affiliation_string":"Laboratoire de Biochimie et de G\u00e9nie Enzymatique des Lipases","institution_ids":["https://openalex.org/I4210111777"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5046706678","display_name":"Fatma Khrouf","orcid":"https://orcid.org/0009-0002-0442-0494","observed_orcids":["https://orcid.org/0009-0002-0442-0494"]},"institutions":[{"id":"https://openalex.org/I4210111777","display_name":"Laboratoire de Biochimie","ror":"https://ror.org/01thw2g46","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I190752583","https://openalex.org/I2746051580","https://openalex.org/I4210111777","https://openalex.org/I4210139971","https://openalex.org/I4210161506","https://openalex.org/I98910050"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Fatma Khrouf","raw_affiliation_strings":["Laboratoire de Biochimie et de G\u00e9nie Enzymatique des Lipases, Sfax, Tunisia","Laboratoire de Biochimie et de G\u00e9nie Enzymatique des Lipases"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratoire de Biochimie et de G\u00e9nie Enzymatique des Lipases, Sfax, Tunisia","institution_ids":[]},{"raw_affiliation_string":"Laboratoire de Biochimie et de G\u00e9nie Enzymatique des Lipases","institution_ids":["https://openalex.org/I4210111777"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5065291278","display_name":"Ahmed Fendri","orcid":"https://orcid.org/0000-0001-5199-1893","observed_orcids":["https://orcid.org/0000-0001-5199-1893"]},"institutions":[{"id":"https://openalex.org/I4210111777","display_name":"Laboratoire de Biochimie","ror":"https://ror.org/01thw2g46","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I190752583","https://openalex.org/I2746051580","https://openalex.org/I4210111777","https://openalex.org/I4210139971","https://openalex.org/I4210161506","https://openalex.org/I98910050"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Ahmed Fendri","raw_affiliation_strings":["Laboratoire de Biochimie et de G\u00e9nie Enzymatique des Lipases, Sfax, Tunisia","Laboratoire de Biochimie et de G\u00e9nie Enzymatique des Lipases"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratoire de Biochimie et de G\u00e9nie Enzymatique des Lipases, Sfax, Tunisia","institution_ids":[]},{"raw_affiliation_string":"Laboratoire de Biochimie et de G\u00e9nie Enzymatique des Lipases","institution_ids":["https://openalex.org/I4210111777"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5022065638","display_name":"Tahar Mechichi","orcid":"https://orcid.org/0000-0001-5602-4528","observed_orcids":["https://orcid.org/0000-0001-5602-4528"]},"institutions":[{"id":"https://openalex.org/I4210111777","display_name":"Laboratoire de Biochimie","ror":"https://ror.org/01thw2g46","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I190752583","https://openalex.org/I2746051580","https://openalex.org/I4210111777","https://openalex.org/I4210139971","https://openalex.org/I4210161506","https://openalex.org/I98910050"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Tahar Mechichi","raw_affiliation_strings":["Laboratoire de Biochimie et de G\u00e9nie Enzymatique des Lipases, Sfax, Tunisia","Laboratoire de Biochimie et de G\u00e9nie Enzymatique des Lipases"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratoire de Biochimie et de G\u00e9nie Enzymatique des Lipases, Sfax, Tunisia","institution_ids":[]},{"raw_affiliation_string":"Laboratoire de Biochimie et de G\u00e9nie Enzymatique des Lipases","institution_ids":["https://openalex.org/I4210111777"]}]},{"author_position":"middle","author":{"id":"https://openalex.org/A5106082251","display_name":"Youssef Gargouri","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210111777","display_name":"Laboratoire de Biochimie","ror":"https://ror.org/01thw2g46","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I190752583","https://openalex.org/I2746051580","https://openalex.org/I4210111777","https://openalex.org/I4210139971","https://openalex.org/I4210161506","https://openalex.org/I98910050"]}],"countries":["FR"],"is_corresponding":false,"raw_author_name":"Youssef Gargouri","raw_affiliation_strings":["Laboratoire de Biochimie et de G\u00e9nie Enzymatique des Lipases, Sfax, Tunisia","Laboratoire de Biochimie et de G\u00e9nie Enzymatique des Lipases"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratoire de Biochimie et de G\u00e9nie Enzymatique des Lipases, Sfax, Tunisia","institution_ids":[]},{"raw_affiliation_string":"Laboratoire de Biochimie et de G\u00e9nie Enzymatique des Lipases","institution_ids":["https://openalex.org/I4210111777"]}]},{"author_position":"last","author":{"id":"https://openalex.org/A5040786260","display_name":"Sofiane Bezzine","orcid":null,"observed_orcids":[]},"institutions":[{"id":"https://openalex.org/I4210111777","display_name":"Laboratoire de Biochimie","ror":"https://ror.org/01thw2g46","country_code":"FR","type":"facility","lineage":["https://openalex.org/I1294671590","https://openalex.org/I190752583","https://openalex.org/I2746051580","https://openalex.org/I4210111777","https://openalex.org/I4210139971","https://openalex.org/I4210161506","https://openalex.org/I98910050"]}],"countries":["FR"],"is_corresponding":true,"raw_author_name":"Sofiane Bezzine","raw_affiliation_strings":["Laboratoire de Biochimie et de G\u00e9nie Enzymatique des Lipases, Sfax, Tunisia","Laboratoire de Biochimie et de G\u00e9nie Enzymatique des Lipases"],"raw_orcid":null,"affiliations":[{"raw_affiliation_string":"Laboratoire de Biochimie et de G\u00e9nie Enzymatique des Lipases, Sfax, Tunisia","institution_ids":[]},{"raw_affiliation_string":"Laboratoire de Biochimie et de G\u00e9nie Enzymatique des Lipases","institution_ids":["https://openalex.org/I4210111777"]}]}],"institutions":[],"countries_distinct_count":1,"institutions_distinct_count":1,"corresponding_author_ids":["https://openalex.org/A5040786260"],"corresponding_institution_ids":["https://openalex.org/I4210111777"],"apc_list":{"value":3890,"currency":"USD","value_usd":3890},"apc_paid":null,"fwci":1.0988,"has_fulltext":false,"cited_by_count":25,"citation_normalized_percentile":{"value":0.68568813,"is_in_top_1_percent":false,"is_in_top_10_percent":false},"cited_by_percentile_year":{"min":87,"max":98},"biblio":{"volume":"168","issue":"8","first_page":"2330","last_page":"2343"},"is_retracted":false,"is_paratext":false,"is_xpac":false,"primary_topic":{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.923799991607666,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},"topics":[{"id":"https://openalex.org/T11617","display_name":"Enzyme Production and Characterization","score":0.923799991607666,"subfield":{"id":"https://openalex.org/subfields/1305","display_name":"Biotechnology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T10404","display_name":"Enzyme Catalysis and Immobilization","score":0.061900001019239426,"subfield":{"id":"https://openalex.org/subfields/1312","display_name":"Molecular Biology"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}},{"id":"https://openalex.org/T11844","display_name":"Lipid metabolism and biosynthesis","score":0.00419999985024333,"subfield":{"id":"https://openalex.org/subfields/1303","display_name":"Biochemistry"},"field":{"id":"https://openalex.org/fields/13","display_name":"Biochemistry, Genetics and Molecular Biology"},"domain":{"id":"https://openalex.org/domains/1","display_name":"Life Sciences"}}],"keywords":[{"id":"https://openalex.org/keywords/fusarium-solani","display_name":"Fusarium solani","score":0.9409999847412109},{"id":"https://openalex.org/keywords/lipase","display_name":"lipase","score":0.703000009059906},{"id":"https://openalex.org/keywords/enzyme-purification","display_name":"enzyme purification","score":0.6589999794960022},{"id":"https://openalex.org/keywords/enzyme-stability","display_name":"enzyme stability","score":0.5149999856948853},{"id":"https://openalex.org/keywords/egg-phosphatidylcholine","display_name":"egg phosphatidylcholine","score":0.8450000286102295},{"id":"https://openalex.org/keywords/olive-oil-emulsion","display_name":"olive oil emulsion","score":0.9620000123977661}],"concepts":[{"id":"https://openalex.org/C2779697368","wikidata":"https://www.wikidata.org/wiki/Q380967","display_name":"Lipase","level":3,"score":0.8695498704910278},{"id":"https://openalex.org/C2778342517","wikidata":"https://www.wikidata.org/wiki/Q5509914","display_name":"Fusarium solani","level":2,"score":0.8478670716285706},{"id":"https://openalex.org/C43617362","wikidata":"https://www.wikidata.org/wiki/Q170050","display_name":"Chromatography","level":1,"score":0.6047320365905762},{"id":"https://openalex.org/C193363816","wikidata":"https://www.wikidata.org/wiki/Q1617865","display_name":"Size-exclusion chromatography","level":3,"score":0.5944517254829407},{"id":"https://openalex.org/C55493867","wikidata":"https://www.wikidata.org/wiki/Q7094","display_name":"Biochemistry","level":1,"score":0.5438249111175537},{"id":"https://openalex.org/C2776428949","wikidata":"https://www.wikidata.org/wiki/Q18814659","display_name":"Ammonium sulfate precipitation","level":4,"score":0.5384407639503479},{"id":"https://openalex.org/C185592680","wikidata":"https://www.wikidata.org/wiki/Q2329","display_name":"Chemistry","level":0,"score":0.5087118744850159},{"id":"https://openalex.org/C58121356","wikidata":"https://www.wikidata.org/wiki/Q182854","display_name":"Molecular mass","level":3,"score":0.49590834975242615},{"id":"https://openalex.org/C2776330855","wikidata":"https://www.wikidata.org/wiki/Q650187","display_name":"Phosphatidylcholine","level":4,"score":0.47726744413375854},{"id":"https://openalex.org/C101660505","wikidata":"https://www.wikidata.org/wiki/Q898301","display_name":"Polyacrylamide gel electrophoresis","level":3,"score":0.46028628945350647},{"id":"https://openalex.org/C181199279","wikidata":"https://www.wikidata.org/wiki/Q8047","display_name":"Enzyme","level":2,"score":0.45885613560676575},{"id":"https://openalex.org/C12927208","wikidata":"https://www.wikidata.org/wiki/Q210430","display_name":"Phospholipase","level":3,"score":0.45554888248443604},{"id":"https://openalex.org/C2780786110","wikidata":"https://www.wikidata.org/wiki/Q7839871","display_name":"Triacylglycerol lipase","level":4,"score":0.451894074678421},{"id":"https://openalex.org/C53941386","wikidata":"https://www.wikidata.org/wiki/Q2571416","display_name":"Ethanol precipitation","level":3,"score":0.4349084496498108},{"id":"https://openalex.org/C116084860","wikidata":"https://www.wikidata.org/wiki/Q48255","display_name":"Gel electrophoresis","level":2,"score":0.4259936809539795},{"id":"https://openalex.org/C537181965","wikidata":"https://www.wikidata.org/wiki/Q658","display_name":"Sodium","level":2,"score":0.411062628030777},{"id":"https://openalex.org/C86803240","wikidata":"https://www.wikidata.org/wiki/Q420","display_name":"Biology","level":0,"score":0.3210303783416748},{"id":"https://openalex.org/C2778918659","wikidata":"https://www.wikidata.org/wiki/Q186915","display_name":"Phospholipid","level":3,"score":0.1946375072002411},{"id":"https://openalex.org/C89423630","wikidata":"https://www.wikidata.org/wiki/Q7193","display_name":"Microbiology","level":1,"score":0.17075008153915405},{"id":"https://openalex.org/C100817775","wikidata":"https://www.wikidata.org/wiki/Q134219","display_name":"Polysaccharide","level":2,"score":0.11375004053115845},{"id":"https://openalex.org/C178790620","wikidata":"https://www.wikidata.org/wiki/Q11351","display_name":"Organic chemistry","level":1,"score":0.08305448293685913},{"id":"https://openalex.org/C41625074","wikidata":"https://www.wikidata.org/wiki/Q176088","display_name":"Membrane","level":2,"score":0.07168522477149963}],"mesh":[{"descriptor_ui":"D000077403","descriptor_name":"Orlistat","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D000595","descriptor_name":"Amino Acid Sequence","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D001647","descriptor_name":"Bile Acids and Salts","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D002118","descriptor_name":"Calcium","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D004795","descriptor_name":"Enzyme Stability","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D005670","descriptor_name":"Fusarium","qualifier_ui":"Q000201","qualifier_name":"enzymology","is_major_topic":true},{"descriptor_ui":"D005670","descriptor_name":"Fusarium","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D006863","descriptor_name":"Hydrogen-Ion Concentration","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D007783","descriptor_name":"Lactones","qualifier_ui":"Q000494","qualifier_name":"pharmacology","is_major_topic":false},{"descriptor_ui":"D010740","descriptor_name":"Phospholipases","qualifier_ui":"Q000302","qualifier_name":"isolation & purification","is_major_topic":true},{"descriptor_ui":"D010740","descriptor_name":"Phospholipases","qualifier_ui":"Q000378","qualifier_name":"metabolism","is_major_topic":true},{"descriptor_ui":"D010740","descriptor_name":"Phospholipases","qualifier_ui":"Q000737","qualifier_name":"chemistry","is_major_topic":false},{"descriptor_ui":"D013696","descriptor_name":"Temperature","qualifier_ui":null,"qualifier_name":null,"is_major_topic":false},{"descriptor_ui":"D014197","descriptor_name":"Trees","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":false},{"descriptor_ui":"D014934","descriptor_name":"Wood","qualifier_ui":"Q000382","qualifier_name":"microbiology","is_major_topic":false},{"descriptor_ui":"D055162","descriptor_name":"Biocatalysis","qualifier_ui":"Q000187","qualifier_name":"drug effects","is_major_topic":false}],"locations_count":2,"locations":[{"id":"doi:10.1007/s12010-012-9940-0","doi":"https://doi.org/10.1007/s12010-012-9940-0","is_oa":false,"landing_page_url":"https://doi.org/10.1007/s12010-012-9940-0","pdf_url":null,"source":{"id":"https://openalex.org/S153285774","display_name":"Applied Biochemistry and Biotechnology","issn_l":"0273-2289","issn":["0273-2289","1559-0291"],"is_oa":false,"is_in_doaj":false,"is_core":true,"listed_in":["cwts-core","dongbi-d","doyens","jufo-1","ki-jl-1","medline","norway-1","poland-70","russia-white-list-2"],"host_organization":"https://openalex.org/P4310319900","host_organization_name":"Springer Science+Business Media","host_organization_lineage":["https://openalex.org/P4310319900","https://openalex.org/P4310319965"],"host_organization_lineage_names":["Springer Science+Business Media","Springer Nature"],"type":"journal"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied Biochemistry and Biotechnology","raw_type":"journal-article"},{"id":"pmid:23151966","doi":null,"is_oa":false,"landing_page_url":"https://pubmed.ncbi.nlm.nih.gov/23151966","pdf_url":null,"source":{"id":"https://openalex.org/S4306525036","display_name":"PubMed","issn_l":null,"issn":null,"is_oa":false,"is_in_doaj":false,"is_core":false,"listed_in":[],"host_organization":"https://openalex.org/I1299303238","host_organization_name":"National Institutes of Health","host_organization_lineage":["https://openalex.org/I1299303238"],"host_organization_lineage_names":[],"type":"repository"},"license":null,"license_id":null,"version":"publishedVersion","is_accepted":true,"is_published":true,"raw_source_name":"Applied biochemistry and biotechnology","raw_type":"Journal Article"}],"best_oa_location":null,"sustainable_development_goals":[],"study_designs":[],"awards":[],"funders":[],"has_content":{"grobid_xml":false,"pdf":false},"content_urls":null,"referenced_works_count":53,"referenced_works":["https://openalex.org/W68494142","https://openalex.org/W94268851","https://openalex.org/W1495142109","https://openalex.org/W1519862364","https://openalex.org/W1600871831","https://openalex.org/W1762307288","https://openalex.org/W1780200189","https://openalex.org/W1902233018","https://openalex.org/W1963722789","https://openalex.org/W1974965654","https://openalex.org/W1977878334","https://openalex.org/W1979546097","https://openalex.org/W1981508058","https://openalex.org/W1983547248","https://openalex.org/W1994353696","https://openalex.org/W2001783660","https://openalex.org/W2004552785","https://openalex.org/W2009009132","https://openalex.org/W2013377060","https://openalex.org/W2013812826","https://openalex.org/W2016932522","https://openalex.org/W2019235724","https://openalex.org/W2028875959","https://openalex.org/W2032087664","https://openalex.org/W2037938119","https://openalex.org/W2042573385","https://openalex.org/W2059961518","https://openalex.org/W2061498308","https://openalex.org/W2063002060","https://openalex.org/W2064696937","https://openalex.org/W2067146729","https://openalex.org/W2067821570","https://openalex.org/W2067975797","https://openalex.org/W2068949242","https://openalex.org/W2077416030","https://openalex.org/W2079186398","https://openalex.org/W2080678749","https://openalex.org/W2082806225","https://openalex.org/W2083598389","https://openalex.org/W2100837269","https://openalex.org/W2110531064","https://openalex.org/W2114038147","https://openalex.org/W2115137329","https://openalex.org/W2121517534","https://openalex.org/W2128635872","https://openalex.org/W2149687154","https://openalex.org/W2156713103","https://openalex.org/W2158086217","https://openalex.org/W2323743941","https://openalex.org/W2397747554","https://openalex.org/W2510236000","https://openalex.org/W6638295461","https://openalex.org/W7045828069"],"related_works":["https://openalex.org/W4377089376","https://openalex.org/W1989735158","https://openalex.org/W2061371058","https://openalex.org/W2519613189","https://openalex.org/W7214253231","https://openalex.org/W7151907788","https://openalex.org/W2072646078","https://openalex.org/W2138911915","https://openalex.org/W2129848331","https://openalex.org/W2586153482"],"abstract_inverted_index":null,"counts_by_year":[{"year":2026,"cited_by_count":1},{"year":2024,"cited_by_count":1},{"year":2023,"cited_by_count":4},{"year":2022,"cited_by_count":2},{"year":2021,"cited_by_count":1},{"year":2020,"cited_by_count":3},{"year":2019,"cited_by_count":2},{"year":2018,"cited_by_count":2},{"year":2016,"cited_by_count":5},{"year":2015,"cited_by_count":3},{"year":2014,"cited_by_count":1}],"updated_date":"2026-11-07T11:25:07.637180","created_date":"2016-06-24T00:00:00"}